Restriction map for
pFN21AE1035
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BamHI BbeI BbrPI
BbsI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RsrII SacII
SapI SbfI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 181 GTCGAC GT^[AC][GT]AC
AfaI 792 GTAC GT^AC
Aor51HI 401 AGCGCT AGC^GCT
Asp700I 637 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 25 GAGCTC G[AT]GC[AT]^C
AvaI 365 CTCGGG C^[CT]CG[GA]G
BanI 1144 GGCACC G^G[CT][GA]CC
BbvCI 79 GCTGAGG CCTCAGC(-5/-2)
BcgI 1049 GCATGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 524 GTATCC GTATCC(6/5)
BcnI 171 CCGGG CC^[GC]GG
BglI 1136 GCCGGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 743 AGATCT A^GATCT
BsaAI 796 TACGTG [CT]AC^GT[GA]
BsaBI 1251 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 824 ACCGGA [AT]^CCGG[AT]
BsiHKAI 25 GAGCTC G[AT]GC[AT]^C
BsmBI 760 GAGACG CGTCTC(1/5)
BsmFI 511 GGGAC GGGAC(10/14)
BsoBI 365 CTCGGG C^[CT]CG[GA]G
BspHI 671 TCATGA T^CATGA
BsrBI 1060 GAGCGG CCGCTC(-3/-3)
BsrFI 1136 GCCGGT [GA]^CCGG[CT]
BssSI 658 CACGAG CACGAG(-5/-1)
BstAPI 777 GCATGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 402 GCGC GCG^C
Cfr10I 1136 GCCGGT [GA]^CCGG[CT]
DraI 997 TTTAAA TTT^AAA
DraII 83 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 954 CCGTGG C^C[GA][CT]GG
EarI 472 GAAGAG CTCTTC(1/4)
Eco47III 401 AGCGCT AGC^GCT
EcoNI 255 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 83 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1016 GAATTC G^AATTC
HaeII 401 AGCGCT [GA]GCGC^[CT]
HhaI 402 GCGC GCG^C
HinP1I 402 GCGC G^CGC
Ksp632I 472 GAAGAG CTCTTC(1/4)
MaeII 797 ACGT A^CGT
MamI 1251 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1164 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 171 CCGGG CC^[GC]GG
PflMI 325 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 513 GACTC GAGTC(4/5)
PshAI 616 GACATGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 671 TCATGA T^CATGA
RsaI 792 GTAC GT^AC
SacI 25 GAGCTC GAGCT^C
SalI 181 GTCGAC G^TCGAC
ScaI 791 AGTACT AGT^ACT
SexAI 863 ACCAGGT A^CC[AT]GGT
SpeI 918 ACTAGT A^CTAGT
SspI 1228 AATATT AAT^ATT
TfiI 1099 GATTC G^A[AT]TC
Van91I 325 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 637 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 638 AAATTT [GA]^AATT[CT]
1016 GAATTC
AlwNI 59 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
326 CAGTTACTG
AocI 229 CCTCAGG CC^T[ACGT]AGG
1093 CCTGAGG
ApoI 638 AAATTT [GA]^AATT[CT]
1016 GAATTC
BalI 302 TGGCCA TGG^CCA
1124 TGGCCA
BanII 25 GAGCTC G[GA]GC[CT]^C
108 GGGCTC
BfaI 529 CTAG C^TAG
919 CTAG
BmyI 25 GAGCTC G[AGT]GC[ACT]^C
108 GGGCTC
BpmI 515 CTCCAG CTGGAG(16/14)
319 CTGGAG
BseRI 1260 CTCCTC GAGGAG(10/8)
970 GAGGAG
BsgI 889 GTGCAG GTGCAG(16/14)
1207 GTGCAG
Bsp1286I 25 GAGCTC G[AGT]GC[ACT]^C
108 GGGCTC
BstXI 126 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1148 CCAAGGGCTTGG
Bsu36I 229 CCTCAGG CC^T[ACGT]AGG
1093 CCTGAGG
Cac8I 494 GCCCGC GC[ACGT]^[ACGT]GC
1132 GCAGGC
Eco81I 229 CCTCAGG CC^T[ACGT]AGG
1093 CCTGAGG
EcoT14I 905 CCTTGG C^C[AT][AT]GG
1148 CCAAGG
FauI 495 CCCGC CCCGC(4/6)
1062 GCGGG
HgaI 771 GACGC GACGC(5/10)
621 GCGTC
HincII 181 GTCGAC GT[CT]^[GA]AC
768 GTTGAC
HindII 181 GTCGAC GT[CT]^[GA]AC
768 GTTGAC
HinfI 513 GACTC G^A[ACGT]TC
1099 GATTC
MaeI 529 CTAG C^TAG
919 CTAG
MluNI 302 TGGCCA TGG^CCA
1124 TGGCCA
MscI 302 TGGCCA TGG^CCA
1124 TGGCCA
PvuII 62 CAGCTG CAG^CTG
316 CAGCTG
StyI 905 CCTTGG C^C[AT][AT]GG
1148 CCAAGG
Tsp45I 143 GTGAC ^GT[GC]AC
359 GTCAC
XcmI 236 CCATTCTGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
266 CCAAGTCGGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BsmAI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
SmlI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII