Restriction map for
pFN21AE2547
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsgI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI DsaI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HpaI KasI KpnI KspI MamI
MluI MluNI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 739 CGCG CG^CG
AccIII 1099 TCCGGA T^CCGGA
AfaI 206 GTAC GT^AC
Alw44I 662 GTGCAC G^TGCAC
ApaLI 662 GTGCAC G^TGCAC
AvaI 250 CCCGAG C^[CT]CG[GA]G
BamHI 1285 GGATCC G^GATCC
BbvI 1350 GCTGC GCAGC(8/12)
BcgI 375 CGAATCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 103 AGATCT A^GATCT
BpmI 986 CTGGAG CTGGAG(16/14)
BsaWI 1099 TCCGGA [AT]^CCGG[AT]
BseAI 1099 TCCGGA T^CCGGA
BseRI 875 CTCCTC GAGGAG(10/8)
BsiEI 891 CGGCCG CG[GA][CT]^CG
BsoBI 250 CCCGAG C^[CT]CG[GA]G
BspEI 1099 TCCGGA T^CCGGA
BspHI 1242 TCATGA T^CATGA
BsrBI 370 CCGCTC CCGCTC(-3/-3)
BstUI 739 CGCG CG^CG
CfoI 255 GCGC GCG^C
DdeI 641 CTGAG C^T[ACGT]AG
DraI 97 TTTAAA TTT^AAA
DraII 329 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1088 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 891 CGGCCG [CT]^GGCC[GA]
EagI 891 CGGCCG C^GGCCG
EclXI 891 CGGCCG C^GGCCG
Eco52I 891 CGGCCG C^GGCCG
EcoO109I 329 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 320 CCTTGG C^C[AT][AT]GG
HhaI 255 GCGC GCG^C
HinP1I 255 GCGC G^CGC
HindIII 535 AAGCTT A^AGCTT
MaeII 660 ACGT A^CGT
MfeI 287 CAATTG C^AATTG
MroI 1099 TCCGGA T^CCGGA
MslI 1061 CATCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1222 CAGCGG C[AC]G^C[GT]G
MunI 287 CAATTG C^AATTG
MvnI 739 CGCG CG^CG
NdeI 1136 CATATG CA^TATG
PflMI 1087 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1242 TCATGA T^CATGA
RsaI 206 GTAC GT^AC
SacI 908 GAGCTC GAGCT^C
SfaNI 382 GATGC GCATC(5/9)
SspI 190 AATATT AAT^ATT
StyI 320 CCTTGG C^C[AT][AT]GG
TseI 1350 GCTGC G^C[AT]GC
Tsp45I 422 GTGAC ^GT[GC]AC
Van91I 1087 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 52 GAATTT [GA]^AATT[CT]
296 AAATTC
AcyI 743 GACGCC G[GA]^CG[CT]C
809 GGCGTC
ApoI 52 GAATTT [GA]^AATT[CT]
296 AAATTC
AspHI 662 GTGCAC G[AT]GC[AT]^C
908 GAGCTC
BcnI 880 CCCGG CC^[GC]GG
894 CCGGG
BsaHI 743 GACGCC G[GA]^CG[CT]C
809 GGCGTC
BsaJI 320 CCTTGG C^C[ACGT][ACGT]GG
894 CCGGGG
BsiHKAI 662 GTGCAC G[AT]GC[AT]^C
908 GAGCTC
BsrI 221 CCAGT ACTGG(1/-1)
748 CCAGT
BstF5I 312 CATCC GGATG(2/0)
624 CATCC
BstXI 1060 CCATCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1184 CCAAAGATTTGG
Cac8I 332 GCCTGC GC[ACGT]^[ACGT]GC
362 GCCAGC
EcoNI 853 CCTCGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1276 CCTTTAGAAGG
FauI 369 CCCGC CCCGC(4/6)
765 GCGGG
Fnu4HI 890 GCGGC GC^[ACGT]GC
1350 GCTGC
FokI 312 CATCC GGATG(9/13)
624 CATCC
HgaI 743 GACGC GACGC(5/10)
810 GCGTC
Hin1I 743 GACGCC G[GA]^CG[CT]C
809 GGCGTC
ItaI 890 GCGGC GC^[ACGT]GC
1350 GCTGC
MwoI 248 GCCCCGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
810 GCGTCCCATGC
NciI 880 CCCGG CC^[GC]GG
894 CCGGG
XbaI 106 TCTAGA T^CTAGA
826 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BfaI BmyI BsaI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BssKI
BstNI BstYI Cfr13I DpnII EarI
EcoRII HaeIII HapII HinfI HpaII
HphI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfcI
TaqI TfiI Tru9I Tsp509I TspRI
TthHB8I XhoII