################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Alw44I 601 GTGCAC G^TGCAC AlwNI 191 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 344 AGCGCT AGC^GCT ApaLI 601 GTGCAC G^TGCAC Asp700I 693 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 601 GTGCAC G[AT]GC[AT]^C BalI 2 TGGCCA TGG^CCA BcgI 411 GCAGATACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 627 CCGGG CC^[GC]GG BfaI 499 CTAG C^TAG BmyI 601 GTGCAC G[AGT]GC[ACT]^C BseRI 223 GAGGAG GAGGAG(10/8) BsiHKAI 601 GTGCAC G[AT]GC[AT]^C BsmFI 629 GGGAC GGGAC(10/14) Bsp1286I 601 GTGCAC G[AGT]GC[ACT]^C BspMI 80 ACCTGC ACCTGC(4/8) BsrBI 226 GAGCGG CCGCTC(-3/-3) BssSI 604 CACGAG CACGAG(-5/-1) BstAPI 594 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 390 GGTCACC G^GT[ACGT]ACC BstPI 390 GGTCACC G^GT[ACGT]ACC BstYI 653 AGATCC [GA]^GATC[CT] Cac8I 138 GCCAGC GC[ACGT]^[ACGT]GC CfoI 345 GCGC GCG^C DraI 129 TTTAAA TTT^AAA EaeI 2 TGGCCA [CT]^GGCC[GA] Eco47III 344 AGCGCT AGC^GCT EcoO65I 390 GGTCACC G^GT[ACGT]ACC EcoT14I 35 CCAAGG C^C[AT][AT]GG EcoT22I 122 ATGCAT ATGCA^T HaeII 344 AGCGCT [GA]GCGC^[CT] HhaI 345 GCGC GCG^C HinP1I 345 GCGC G^CGC MaeI 499 CTAG C^TAG MflI 653 AGATCC [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MseI 130 TTAA T^TAA NciI 627 CCGGG CC^[GC]GG NsiI 122 ATGCAT ATGCA^T PpuMI 291 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 286 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC StyI 35 CCAAGG C^C[AT][AT]GG TaqI 701 TCGA T^CGA Tru9I 130 TTAA T^TAA Tsp509I 439 AATT ^AATT TthHB8I 701 TCGA T^CGA XhoII 653 AGATCC [GA]^GATC[CT] XmnI 693 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 657 CCGC CCGC(-3/-1) 228 GCGG AfaI 65 GTAC GT^AC 624 GTAC AlwI 199 GATCC GGATC(4/5) 654 GATCC AvaI 22 CCCGAG C^[CT]CG[GA]G 85 CTCGGG BsoBI 22 CCCGAG C^[CT]CG[GA]G 85 CTCGGG BsrDI 317 GCAATG GCAATG(2/0) 659 GCAATG DraII 173 AGGCCCC [GA]G^G[ACGT]CC[CT] 291 AGGTCCT EcoO109I 173 AGGCCCC [GA]G^G[ACGT]CC[CT] 291 AGGTCCT HapII 627 CCGG C^CGG 636 CCGG HpaII 627 CCGG C^CGG 636 CCGG MslI 186 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 577 CATGTGGGTG MspA1I 406 CAGCTG C[AC]G^C[GT]G 595 CAGCTG MspI 627 CCGG C^CGG 636 CCGG PstI 340 CTGCAG CTGCA^G 409 CTGCAG PvuII 406 CAGCTG CAG^CTG 595 CAGCTG RsaI 65 GTAC GT^AC 624 GTAC SexAI 386 ACCTGGT A^CC[AT]GGT 397 ACCTGGT SfaNI 121 GATGC GCATC(5/9) 547 GATGC SfcI 340 CTGCAG C^T[GA][CT]AG 409 CTGCAG SmlI 159 CTTGAG C^T[CT][GA]AG 709 CTCAAG Tsp45I 391 GTCAC ^GT[GC]AC 553 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AvaII BbsI BbvI BpuAI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI TspRI