Restriction map for pFN21AE1452
 (Circular) (Six-base) MAPSORT of: pFN21AE1452.seq  Check: 9496  from: 1  to: 5452



pFN21AE1452  5452 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:53  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3262     278 
   Size:         53      83     186     936    1726    2468

  Fragments arranged by size: 

                2468    1726     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2795    1937 
   Size:        858    4594
 
Acc65I G'GTAC_C

Cuts at:   2773    2773 
   Size:       5452
 
AclI AA'CG_TT

Cuts at:   1563    3579    3952    1563 
   Size:       2016     373    3063

  Fragments arranged by size: 

                3063    2016     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2195    2717    3515    4899    5167    1632 
   Size:        563     522     798    1384     268    1917

  Fragments arranged by size: 

                1917    1384     798     563     522     268
 
AfeI AGC'GCT

Cuts at:   2351    2351 
   Size:       5452
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5255
 
AflIII A'CryG_T

Cuts at:   1203    4351    5328    1203 
   Size:       3148     977    1327

  Fragments arranged by size: 

                3148    1327     977
 
AgeI A'CCGG_T

Cuts at:   4283    4283 
   Size:       5452
 
AhdI GACnn_n'nnGTC

Cuts at:   4181    4181 
   Size:       5452
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5452
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5452
 
AlwNI CAG_nnn'CTG

Cuts at:   2201    3195    4767    2201 
   Size:        994    1572    2886

  Fragments arranged by size: 

                2886    1572     994
 
ApaLI G'TGCA_C

Cuts at:   2606    3509    4665    2606 
   Size:        903    1156    3393

  Fragments arranged by size: 

                3393    1156     903
 
ApoI r'AATT_y

Cuts at:   1493    2761    2987    3139    5248    1493 
   Size:       1268     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1268     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4006     160 
   Size:        872    2974    1606

  Fragments arranged by size: 

                2974    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5452
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2027    2090    2777    1870 
   Size:         73      84      63     687    4545

  Fragments arranged by size: 

                4545     687      84      73      63
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2053    2086    2053 
   Size:         33    5419
 
BamHI G'GATC_C

Cuts at:   2782    3149    2782 
   Size:        367    5085
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2773    4228     618 
   Size:        325     217     627     986    1455    1842

  Fragments arranged by size: 

                1842    1455     986     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2771    1391 
   Size:       1380    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2067    2262    2295     928 
   Size:        971     168     195      33    4085

  Fragments arranged by size: 

                4085     971     195     168      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2405    2439    3643    3677    1903 
   Size:         34     468      34    1204      34    3678

  Fragments arranged by size: 

                3678    1204     468      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3344    4554    3344 
   Size:       1210    4242
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5452
 
BfrBI ATG'CAT

Cuts at:   2129    2129 
   Size:       5452
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4063     136 
   Size:        107     122      71     631     605    2391    1525

  Fragments arranged by size: 

                2391    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5447    5447 
   Size:       5452
 
BlpI GC'TnA_GC

Cuts at:   2869    2869 
   Size:       5452
 
Bme1580I G_kGCm'C

Cuts at:   1790    2610    3513    4669    1790 
   Size:        820     903    1156    2573

  Fragments arranged by size: 

                2573    1156     903     820
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5452
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2189    2306    3183 
   Size:        416      53      83     399      24     772     117     877

Cuts at:   3183    4141     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753     958     877     772     416     399     117      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5452
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4112    1239 
   Size:       2873    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2015    2015 
   Size:       5452
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2185    2699    3577    4442    4740    2185 
   Size:        514     878     865     298    2897

  Fragments arranged by size: 

                2897     878     865     514     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4115     882 
   Size:        617    2616    2219

  Fragments arranged by size: 

                2616    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3243     493 
   Size:        711    2039    2702

  Fragments arranged by size: 

                2702    2039     711
 
BsaBI GATnn'nnATC

Cuts at:   5446    5446 
   Size:       5452
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3259    3641    4276     275 
   Size:         53      83     186     936    1726     382     635    1451

  Fragments arranged by size: 

                1726    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3885    4283    4557    4704    5115    3885 
   Size:        398     274     147     411    4222

  Fragments arranged by size: 

                4222     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2442    2456    2472    2486    1178 
   Size:         30     165      30    1039      14      16      14    4144

  Fragments arranged by size: 

                4144    1039     165      30      30      16      14      14
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2243    1186 
   Size:       1057    4395
 
BseYI C'CCAG_C

Cuts at:   4655    5151    4655 
   Size:        496    4956
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4879
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2902    3663    4691    1540 
   Size:        275     186     901     761    1028    2301

  Fragments arranged by size: 

                2301    1028     901     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2610    3513    3598    4669    2610 
   Size:        903      85    1071    3393

  Fragments arranged by size: 

                3393    1071     903      85
 
BsmI GAATG_Cn'

Cuts at:   2963    3056    2963 
   Size:         93    5359
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2610    2771    3513    3598    4669    1391 
   Size:        399     820     161     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     820     742     399     161      85
 
BspEI T'CCGG_A

Cuts at:   5115    5115 
   Size:       5452
 
BspHI T'CATG_A

Cuts at:   3341    5071    3341 
   Size:       1730    3722
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2094    2807     844 
   Size:       1250     713    3489

  Fragments arranged by size: 

                3489    1250     713
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2233    3339    1394 
   Size:        429     410    1106    3507

  Fragments arranged by size: 

                3507    1106     429     410
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2329    2671    3947    4121      65 
   Size:       1183    1081     342    1276     174    1396

  Fragments arranged by size: 

                1396    1276    1183    1081     342     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4096    4283    1700 
   Size:        110    2286     187    2869

  Fragments arranged by size: 

                2869    2286     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5452
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5452
 
BssSI C'ACGA_G

Cuts at:   2609    3512    4524    2609 
   Size:        903    1012    3537

  Fragments arranged by size: 

                3537    1012     903
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2605     794 
   Size:       1811    3641
 
BstBI TT'CG_AA

Cuts at:   4256    4256 
   Size:       5452
 
BstEII G'GTnAC_C

Cuts at:   1184    2395    1184 
   Size:       1211    4241
 
BstYI r'GATC_y

Cuts at:   1972    2658    2782    3149    3534    3551    5003    5089    5101 
   Size:        686     124     367     385      17    1452      86      12

Cuts at:   5101    5447    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     686     385     367     346     124      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3961

  Fragments arranged by size: 

                3961     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4713

  Fragments arranged by size: 

                4713     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2562    2968    3762    3782    2562 
   Size:        406     794      20    4232

  Fragments arranged by size: 

                4232     794     406      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5452
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5417
 
DraI TTT'AAA

Cuts at:   2136    2756    2136 
   Size:        620    4832
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4459     809 
   Size:        507    3143    1802

  Fragments arranged by size: 

                3143    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2007    2899    3788 
   Size:         54    1425      50     275      18     177     892     889

Cuts at:   3788    5417       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     892     889     275     177      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2899    1537 
   Size:        275    1087    4090

  Fragments arranged by size: 

                4090    1087     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3382    1353 
   Size:       2029    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4039    4413    4559     387 
   Size:       1064     138    2450     374     146    1280

  Fragments arranged by size: 

                2450    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2195    2717    3515    4112    4899    5167    1239 
   Size:        393     563     522     798     597     787     268    1524

  Fragments arranged by size: 

                1524     798     787     597     563     522     393     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2179    2297    2179 
   Size:        118    5334
 
EcoRI G'AATT_C

Cuts at:   2761    3139    2761 
   Size:        378    5074
 
FspI TGC'GCA

Cuts at:   3958    3958 
   Size:       5452
 
HaeII r_GCGC'y

Cuts at:   1678    2353    4599    1678 
   Size:        675    2246    2531

  Fragments arranged by size: 

                2531    2246     675
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2442    2474    4093    4125    4167 
   Size:         32     135      32     870      32    1619      32      42

Cuts at:   4167    4199    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1619     870     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2796    3042     669 
   Size:        988     281     858     246    3079

  Fragments arranged by size: 

                3079     988     858     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5452
 
HpaI GTT'AAC

Cuts at:   3042    3042 
   Size:       5452
 
KpnI G_GTAC'C

Cuts at:   2777    2777 
   Size:       5452
 
MfeI C'AATT_G

Cuts at:   3051    3051 
   Size:       5452
 
MluI A'CGCG_T

Cuts at:   5328    5328 
   Size:       5452
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4566    4750    1305 
   Size:       3261     184    2007

  Fragments arranged by size: 

                3261    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2009      10 
   Size:         54    1425     343     177    3453

  Fragments arranged by size: 

                3453    1425     343     177      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    2195    2586    3410    3769    3928     518 
   Size:        648     843     186     391     824     359     159    2042

  Fragments arranged by size: 

                2042     843     824     648     391     359     186     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    2413    2602    2868    3545    4693    4938    1606 
   Size:        807     189     266     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     807     677     266     245     189
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3961

  Fragments arranged by size: 

                3961     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5452
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5452
 
NotI GC'GGCC_GC

Cuts at:   2899    2899 
   Size:       5452
 
NsiI A_TGCA'T

Cuts at:   2131    2131 
   Size:       5452
 
NspI r_CATG'y

Cuts at:   1132    1229    2810    4355    1132 
   Size:         97    1581    1545    2229

  Fragments arranged by size: 

                2229    1581    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2724    1348 
   Size:       1376    4076
 
PciI A'CATG_T

Cuts at:   4351    4351 
   Size:       5452
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5452
 
PfoI T'CCnGG_A

Cuts at:   2460    2460 
   Size:       5452
 
PmeI GTTT'AAAC

Cuts at:   2756    2756 
   Size:       5452
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3521    3553    5034    5066    3521 
   Size:         32    1481      32    3907

  Fragments arranged by size: 

                3907    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2297    2297 
   Size:       5452
 
PshAI GACnn'nnGTC

Cuts at:   1613    2295    1613 
   Size:        682    4770
 
PsiI TTA'TAA

Cuts at:   3022    3022 
   Size:       5452
 
PstI C_TGCA'G

Cuts at:    830    2349    2418    2804     830 
   Size:       1519      69     386    3478

  Fragments arranged by size: 

                3478    1519     386      69
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5452
 
PvuII CAG'CTG

Cuts at:   2413    2602    2413 
   Size:        189    5263
 
SalI G'TCGA_C

Cuts at:   1936    2794    1936 
   Size:        858    4594
 
SbfI CC_TGCA'GG

Cuts at:   2804    2804 
   Size:       5452
 
ScaI AGT'ACT

Cuts at:   3700    5412    3700 
   Size:       1712    3740
 
SexAI A'CCwGG_T

Cuts at:   2391    2402    2391 
   Size:         11    5441
 
SfcI C'TryA_G

Cuts at:    826    1045    2345    2414    2800    3935    4616    4807     826 
   Size:        219    1300      69     386    1135     681     191    1471

  Fragments arranged by size: 

                1471    1300    1135     681     386     219     191      69
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5452
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5452
 
SmaI CCC'GGG

Cuts at:   1872    2779    1872 
   Size:        907    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2164    2714    3556    4457    4719     820 
   Size:        197     926     221     550     842     901     262    1553

  Fragments arranged by size: 

                1553     926     901     842     550     262     221     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3243     493 
   Size:       2750    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5452
 
SphI G_CATG'C

Cuts at:   1132    2810    1132 
   Size:       1678    3774
 
SspI AAT'ATT

Cuts at:      5      52    3376       5 
   Size:         47    3324    2081

  Fragments arranged by size: 

                3324    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5452
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2040    2891     513 
   Size:        552     939      36     851    3074

  Fragments arranged by size: 

                3074     939     851     552      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2576    3479    3496    3649    3834    4279     325 
   Size:       1255     996     903      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     996     903     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3698    5410      96 
   Size:        274      80      33      51    1443    1721    1712     138

  Fragments arranged by size: 

                1721    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5412    5444    5412 
   Size:         32    5420
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5452
 
XbaI T'CTAG_A

Cuts at:   2788    2788 
   Size:       5452
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5452
 
XmaI C'CCGG_G

Cuts at:   1870    2777    1870 
   Size:        907    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2702    3581    2702 
   Size:        879    4573
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3260     276 
   Size:         53      83     186     936    1726    2468

  Fragments arranged by size: 

                2468    1726     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII     SalI     SbfI     ScaI    SexAI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI     BplI
    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI     SfiI     SfoI     SrfI     SwaI     XcmI
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