Restriction map for
pFN21AE3622
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsgI BsiWI BsmI Bsp1407I
BspDI BspLU11I BspMI BsrFI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MfeI MluI
MseI MspA1I MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
SexAI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 771 TCCGGA T^CCGGA
ApaI 1095 GGGCCC GGGCC^C
AspHI 298 GAGCAC G[AT]GC[AT]^C
AspI 469 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1095 GGGCCC G[GA]GC[CT]^C
BglI 403 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 251 AGATCT A^GATCT
BsaBI 978 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 771 TCCGGA T^CCGGA
BsiEI 608 CGGCCG CG[GA][CT]^CG
BsiHKAI 298 GAGCAC G[AT]GC[AT]^C
BsmBI 467 GAGACG CGTCTC(1/5)
BspEI 771 TCCGGA T^CCGGA
BssSI 823 CACGAG CACGAG(-5/-1)
BstAPI 300 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 474 GGTCACC G^GT[ACGT]ACC
BstPI 474 GGTCACC G^GT[ACGT]ACC
BstXI 392 CCATGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 605 CCACGG C^C[GA][CT]GG
EagI 608 CGGCCG C^GGCCG
EclXI 608 CGGCCG C^GGCCG
Eco52I 608 CGGCCG C^GGCCG
EcoO65I 474 GGTCACC G^GT[ACGT]ACC
EcoT14I 335 CCAAGG C^C[AT][AT]GG
FauI 615 GCGGG CCCGC(4/6)
MamI 978 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 771 TCCGGA T^CCGGA
NspI 44 GCATGT [GA]CATG^[CT]
PflFI 469 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 548 GGGACCT [GA]G^G[AT]CC[CT]
SapI 711 GAAGAGC GCTCTTC(1/4)
ScaI 1007 AGTACT AGT^ACT
SfcI 591 CTACAG C^T[GA][CT]AG
SfiI 402 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 918 CCCGGG CCC^GGG
StyI 335 CCAAGG C^C[AT][AT]GG
TaqI 668 TCGA T^CGA
TfiI 808 GAATC G^A[AT]TC
Tth111I 469 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 668 TCGA T^CGA
XmaCI 918 CCCGGG C^CCGGG
XmaI 918 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 573 GATCC GGATC(4/5)
1019 GGATC
AvaI 80 CCCGAG C^[CT]CG[GA]G
918 CCCGGG
AvaII 534 GGACC G^G[AT]CC
549 GGACC
BbeI 107 GGCGCC GGCGC^C
960 GGCGCC
BpmI 464 CTGGAG CTGGAG(16/14)
706 CTGGAG
BsaAI 157 TACGTG [CT]AC^GT[GA]
397 TACGTG
BsaI 373 GAGACC GGTCTC(1/5)
826 GAGACC
BsaWI 764 TCCGGT [AT]^CCGG[AT]
771 TCCGGA
BseRI 696 CTCCTC GAGGAG(10/8)
295 GAGGAG
BsoBI 80 CCCGAG C^[CT]CG[GA]G
918 CCCGGG
BspHI 566 TCATGA T^CATGA
845 TCATGA
BsrBI 613 GAGCGG CCGCTC(-3/-3)
757 GAGCGG
BsrDI 924 CATTGC GCAATG(2/0)
752 GCAATG
BstYI 251 AGATCT [GA]^GATC[CT]
572 AGATCC
DdeI 359 CTCAG C^T[ACGT]AG
581 CTGAG
DraIII 301 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
481 CACACGGTG
KasI 107 GGCGCC G^GCGCC
960 GGCGCC
MflI 251 AGATCT [GA]^GATC[CT]
572 AGATCC
NarI 107 GGCGCC GG^CGCC
960 GGCGCC
RcaI 566 TCATGA T^CATGA
845 TCATGA
SfoI 107 GGCGCC GGC^GCC
960 GGCGCC
XcmI 174 CCAGAGCAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
521 CCATCCTGCGTCTGG
XhoII 251 AGATCT [GA]^GATC[CT]
572 AGATCC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
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AccII AciI AcyI AfaI AluI
BalI BanI BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DraII EaeI EarI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI