(Circular) (Six-base) MAPSORT of: pFN21AE3622.seq Check: 7275 from: 1 to: 5830 pFN21AE3622 5830 bp With 182 enzymes: SgfI * July 6, 2012 17:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2225 3893 5277 5545 1632 Size: 593 1668 1384 268 1917 Fragments arranged by size: 1917 1668 1384 593 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2771 2803 2771 Size: 32 5798 AlwNI CAG_nnn'CTG Cuts at: 3573 5145 3573 Size: 1572 4258 ApaI G_GGCC'C Cuts at: 3104 3104 Size: 5830 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 3139 3365 3517 5626 1493 Size: 1646 226 152 2109 1697 Fragments arranged by size: 2109 1697 1646 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 2085 2923 3155 1870 Size: 73 142 838 232 4545 Fragments arranged by size: 4545 838 232 142 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 2262 2491 2909 2965 Size: 325 217 627 325 150 229 418 56 Cuts at: 2965 3151 4606 618 Size: 186 1455 1842 Fragments arranged by size: 1842 1455 627 418 325 325 229 217 186 150 56 BanII G_rGCy'C Cuts at: 1391 3104 3149 1391 Size: 1713 45 4072 Fragments arranged by size: 4072 1713 45 BbeI G_GCGC'C Cuts at: 2116 2969 2116 Size: 853 4977 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4859 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3109 3143 4021 4055 1903 Size: 34 1172 34 878 34 3678 Fragments arranged by size: 3678 1172 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2414 4441 136 Size: 107 122 71 631 605 742 2027 1525 Fragments arranged by size: 2027 1525 742 631 605 122 107 71 BglII A'GATC_T Cuts at: 2256 5825 2256 Size: 3569 2261 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2496 3104 3891 5047 1790 Size: 706 608 787 1156 2573 Fragments arranged by size: 2573 1156 787 706 608 BmgBI CAC'GTC Cuts at: 1318 2863 1318 Size: 1545 4285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2248 2303 3561 Size: 416 53 83 399 24 831 55 1258 Cuts at: 3561 4519 442 Size: 958 1753 Fragments arranged by size: 1753 1258 958 831 416 399 83 55 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2490 2732 4490 1239 Size: 1251 242 1758 2579 Fragments arranged by size: 2579 1758 1251 242 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3955 4820 5118 3955 Size: 865 298 4667 Fragments arranged by size: 4667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2372 2825 4493 882 Size: 617 873 453 1668 2219 Fragments arranged by size: 2219 1668 873 617 453 BsaAI yAC'GTr Cuts at: 493 1204 2164 2404 3621 493 Size: 711 960 240 1217 2702 Fragments arranged by size: 2702 1217 960 711 240 BsaBI GATnn'nnATC Cuts at: 2987 5824 2987 Size: 2837 2993 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2113 2130 2966 3637 Size: 53 83 186 936 580 17 836 671 Cuts at: 3637 4019 4654 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 836 671 635 580 382 186 83 53 17 BsaWI w'CCGG_w Cuts at: 2769 2776 4263 4661 4935 5082 5493 2769 Size: 7 1487 398 274 147 411 3106 Fragments arranged by size: 3106 1487 411 398 274 147 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2463 2493 2636 Size: 30 165 30 624 30 406 30 143 Cuts at: 2636 2666 2771 2801 1178 Size: 30 105 30 4207 Fragments arranged by size: 4207 624 406 165 143 105 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2315 2692 1186 Size: 1129 377 4324 Fragments arranged by size: 4324 1129 377 BseYI C'CCAG_C Cuts at: 2069 2969 5033 5529 2069 Size: 900 2064 496 2370 Fragments arranged by size: 2370 2064 900 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2616 3280 4041 5069 1540 Size: 275 186 615 664 761 1028 2301 Fragments arranged by size: 2301 1028 761 664 615 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 3891 3976 5047 2307 Size: 1584 85 1071 3090 Fragments arranged by size: 3090 1584 1071 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 BsmBI CGTCTCn'nnnn_ Cuts at: 2466 2466 Size: 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2307 2496 3104 3149 3891 3976 5047 Size: 399 517 189 608 45 742 85 1071 Cuts at: 5047 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 608 517 399 189 85 45 BspEI T'CCGG_A Cuts at: 2776 5493 2776 Size: 2717 3113 BspHI T'CATG_A Cuts at: 2571 2850 3719 5449 2571 Size: 279 869 1730 2952 Fragments arranged by size: 2952 1730 869 279 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3185 844 Size: 2341 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2620 2764 3717 1394 Size: 429 797 144 953 3507 Fragments arranged by size: 3507 953 797 429 144 BsrDI GCAATG_nn' Cuts at: 65 1248 2764 2928 4325 4499 65 Size: 1183 1516 164 1397 174 1396 Fragments arranged by size: 1516 1397 1396 1183 174 164 BsrFI r'CCGG_y Cuts at: 1700 1810 4474 4661 1700 Size: 110 2664 187 2869 Fragments arranged by size: 2869 2664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 2828 3890 4902 2828 Size: 1062 1012 3756 Fragments arranged by size: 3756 1062 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2311 794 Size: 1517 4313 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 2479 1184 Size: 1295 4535 BstXI CCAn_nnnn'nTGG Cuts at: 2404 2404 Size: 5830 BstYI r'GATC_y Cuts at: 1972 2256 2577 3160 3527 3912 3929 5381 5467 Size: 284 321 583 367 385 17 1452 86 Cuts at: 5467 5479 5825 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 583 385 367 346 321 284 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2610 513 Size: 552 939 606 3733 Fragments arranged by size: 3733 939 606 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2977 487 Size: 683 56 1751 3340 Fragments arranged by size: 3340 1751 683 56 BtsI GCAGTG_nn' Cuts at: 3346 4140 4160 3346 Size: 794 20 5016 Fragments arranged by size: 5016 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2437 2472 529 Size: 35 1873 35 3887 Fragments arranged by size: 3887 1873 35 35 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DraIII CAC_nnn'GTG Cuts at: 2311 2491 2311 Size: 180 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2394 2406 2548 Size: 54 1425 50 275 18 564 12 142 Cuts at: 2548 2613 2685 2737 3277 4166 5795 8 Size: 65 72 52 540 889 1629 43 Fragments arranged by size: 1629 1425 889 564 540 275 142 72 65 54 52 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2613 3277 1537 Size: 275 801 664 4090 Fragments arranged by size: 4090 801 664 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 2710 2711 3760 1353 Size: 654 703 1 1049 3423 Fragments arranged by size: 3423 1049 703 654 1 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2490 2732 3893 4490 5277 5545 Size: 393 593 265 242 1161 597 787 268 Cuts at: 5545 1239 Size: 1524 Fragments arranged by size: 1524 1161 787 597 593 393 268 265 242 EcoO109I rG'GnC_Cy Cuts at: 2326 2554 3101 2326 Size: 228 547 5055 Fragments arranged by size: 5055 547 228 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 2116 2786 2969 4977 1678 Size: 438 670 183 2008 2531 Fragments arranged by size: 2531 2008 670 438 183 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2463 2495 4471 Size: 32 135 32 455 32 404 32 1976 Cuts at: 4471 4503 4545 4577 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1976 455 404 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KasI G'GCGC_C Cuts at: 2112 2965 2112 Size: 853 4977 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 2687 2739 10 Size: 54 1425 343 576 279 52 3101 Fragments arranged by size: 3101 1425 576 343 279 54 52 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2459 2915 3788 4147 4306 518 Size: 648 1236 57 456 873 359 159 2042 Fragments arranged by size: 2042 1236 873 648 456 359 159 57 MspA1I CmG'CkG Cuts at: 1606 3246 3923 5071 5316 1606 Size: 1640 677 1148 245 2120 Fragments arranged by size: 2120 1640 1148 677 245 NarI GG'CG_CC Cuts at: 2113 2966 2113 Size: 853 4977 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4339 Fragments arranged by size: 4339 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2053 3188 4733 1132 Size: 97 824 1135 1545 2229 Fragments arranged by size: 2229 1545 1135 824 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2771 2803 3899 3931 5412 5444 2771 Size: 32 1096 32 1481 32 3157 Fragments arranged by size: 3157 1481 1096 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2554 2554 Size: 5830 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspOMI G'GGCC_C Cuts at: 3100 3100 Size: 5830 PstI C_TGCA'G Cuts at: 830 3182 830 Size: 2352 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2711 2711 Size: 5830 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 3014 4078 5790 3014 Size: 1064 1712 3054 Fragments arranged by size: 3054 1712 1064 SfcI C'TryA_G Cuts at: 826 1045 2596 3178 4313 4994 5185 826 Size: 219 1551 582 1135 681 191 1471 Fragments arranged by size: 1551 1471 1135 681 582 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2414 2414 Size: 5830 SfoI GGC'GCC Cuts at: 2114 2967 2114 Size: 853 4977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 2925 3157 1872 Size: 1053 232 4545 Fragments arranged by size: 4545 1053 232 SmlI C'TyrA_G Cuts at: 820 1017 1943 3934 4835 5097 820 Size: 197 926 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 2004 2340 3269 513 Size: 552 939 336 929 3074 Fragments arranged by size: 3074 939 929 552 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2322 2336 2498 2576 3857 3874 4027 Size: 1255 742 14 162 78 1281 17 153 Cuts at: 4027 4212 4657 325 Size: 185 445 1498 Fragments arranged by size: 1498 1281 1255 742 445 185 162 153 78 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3012 4076 5788 Size: 274 80 33 51 1443 1035 1064 1712 Cuts at: 5788 96 Size: 138 Fragments arranged by size: 1712 1443 1064 1035 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2636 2668 5790 5822 2636 Size: 32 3122 32 2644 Fragments arranged by size: 3122 2644 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2477 1316 Size: 1161 4669 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2186 2533 2186 Size: 347 5483 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 2923 3155 1870 Size: 1053 232 4545 Fragments arranged by size: 4545 1053 232 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SrfI SwaI