(Linear) (Six-base) MAPSORT of: KIEE3622.seq Check: 7955 from: 1 to: 1125 KIEE3622.orf With 182 enzymes: SgfI * July 3, 2012 18:13 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 1125 Size: 220 905 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 766 798 1125 Size: 766 32 327 Fragments arranged by size: 766 327 32 ApaI G_GGCC'C Cuts at: 0 1099 1125 Size: 1099 26 AvaI C'yCGr_G Cuts at: 0 80 918 1125 Size: 80 838 207 Fragments arranged by size: 838 207 80 BanI G'GyrC_C Cuts at: 0 107 257 486 904 960 1125 Size: 107 150 229 418 56 165 Fragments arranged by size: 418 229 165 150 107 56 BanII G_rGCy'C Cuts at: 0 1099 1125 Size: 1099 26 BbeI G_GCGC'C Cuts at: 0 111 964 1125 Size: 111 853 161 Fragments arranged by size: 853 161 111 BglI GCCn_nnn'nGGC Cuts at: 0 409 1125 Size: 409 716 BglII A'GATC_T Cuts at: 0 251 1125 Size: 251 874 Bme1580I G_kGCm'C Cuts at: 0 491 1099 1125 Size: 491 608 26 Fragments arranged by size: 608 491 26 BmgBI CAC'GTC Cuts at: 0 858 1125 Size: 858 267 BmrI ACTGGGnnnn_n' Cuts at: 0 243 298 1125 Size: 243 55 827 Fragments arranged by size: 827 243 55 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 485 727 1125 Size: 485 242 398 Fragments arranged by size: 485 398 242 BsaI GGTCTCn'nnnn_ Cuts at: 0 367 820 1125 Size: 367 453 305 Fragments arranged by size: 453 367 305 BsaAI yAC'GTr Cuts at: 0 159 399 1125 Size: 159 240 726 Fragments arranged by size: 726 240 159 BsaBI GATnn'nnATC Cuts at: 0 982 1125 Size: 982 143 BsaHI Gr'CG_yC Cuts at: 0 108 125 961 1125 Size: 108 17 836 164 Fragments arranged by size: 836 164 108 17 BsaWI w'CCGG_w Cuts at: 0 764 771 1125 Size: 764 7 354 Fragments arranged by size: 764 354 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 22 52 458 488 631 661 766 796 Size: 22 30 406 30 143 30 105 30 Cuts at: 796 1125 Size: 329 Fragments arranged by size: 406 329 143 105 30 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 310 687 1125 Size: 310 377 438 Fragments arranged by size: 438 377 310 BseYI C'CCAG_C Cuts at: 0 64 964 1125 Size: 64 900 161 Fragments arranged by size: 900 161 64 BsiEI CG_ry'CG Cuts at: 0 611 1125 Size: 611 514 BsiHKAI G_wGCw'C Cuts at: 0 302 1125 Size: 302 823 BsmBI CGTCTCn'nnnn_ Cuts at: 0 461 1125 Size: 461 664 Bsp1286I G_dGCh'C Cuts at: 0 302 491 1099 1125 Size: 302 189 608 26 Fragments arranged by size: 608 302 189 26 BspEI T'CCGG_A Cuts at: 0 771 1125 Size: 771 354 BspHI T'CATG_A Cuts at: 0 566 845 1125 Size: 566 279 280 Fragments arranged by size: 566 280 279 BsrBI CCG'CTC Cuts at: 0 615 759 1125 Size: 615 144 366 Fragments arranged by size: 615 366 144 BsrDI GCAATG_nn' Cuts at: 0 759 923 1125 Size: 759 164 202 Fragments arranged by size: 759 202 164 BssSI C'ACGA_G Cuts at: 0 823 1125 Size: 823 302 BstAPI GCAn_nnn'nTGC Cuts at: 0 306 1125 Size: 306 819 BstEII G'GTnAC_C Cuts at: 0 474 1125 Size: 474 651 BstXI CCAn_nnnn'nTGG Cuts at: 0 399 1125 Size: 399 726 BstYI r'GATC_y Cuts at: 0 251 572 1125 Size: 251 321 553 Fragments arranged by size: 553 321 251 BtgI C'CryG_G Cuts at: 0 605 1125 Size: 605 520 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 972 1125 Size: 972 153 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 432 467 1125 Size: 432 35 658 Fragments arranged by size: 658 432 35 DraIII CAC_nnn'GTG Cuts at: 0 306 486 1125 Size: 306 180 639 Fragments arranged by size: 639 306 180 EaeI y'GGCC_r Cuts at: 0 389 401 543 608 680 732 1125 Size: 389 12 142 65 72 52 393 Fragments arranged by size: 393 389 142 72 65 52 12 EagI C'GGCC_G Cuts at: 0 608 1125 Size: 608 517 EarI CTCTTCn'nnn_ Cuts at: 0 2 705 706 1125 Size: 2 703 1 419 Fragments arranged by size: 703 419 2 1 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 485 727 1125 Size: 220 265 242 398 Fragments arranged by size: 398 265 242 220 EcoO109I rG'GnC_Cy Cuts at: 0 321 549 1096 1125 Size: 321 228 547 29 Fragments arranged by size: 547 321 228 29 HaeII r_GCGC'y Cuts at: 0 111 781 964 1125 Size: 111 670 183 161 Fragments arranged by size: 670 183 161 111 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 22 54 458 490 1125 Size: 22 32 404 32 635 Fragments arranged by size: 635 404 32 32 22 KasI G'GCGC_C Cuts at: 0 107 960 1125 Size: 107 853 165 Fragments arranged by size: 853 165 107 MscI TGG'CCA Cuts at: 0 403 682 734 1125 Size: 403 279 52 391 Fragments arranged by size: 403 391 279 52 MslI CAynn'nnrTG Cuts at: 0 397 454 910 1125 Size: 397 57 456 215 Fragments arranged by size: 456 397 215 57 NarI GG'CG_CC Cuts at: 0 108 961 1125 Size: 108 853 164 Fragments arranged by size: 853 164 108 NspI r_CATG'y Cuts at: 0 48 1125 Size: 48 1077 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 766 798 1125 Size: 766 32 327 Fragments arranged by size: 766 327 32 PpuMI rG'GwC_Cy Cuts at: 0 549 1125 Size: 549 576 PspOMI G'GGCC_C Cuts at: 0 1095 1125 Size: 1095 30 SapI GCTCTTCn'nnn_ Cuts at: 0 706 1125 Size: 706 419 ScaI AGT'ACT Cuts at: 0 1009 1125 Size: 1009 116 SfcI C'TryA_G Cuts at: 0 591 1125 Size: 591 534 SfiI GGCCn_nnn'nGGCC Cuts at: 0 409 1125 Size: 409 716 SfoI GGC'GCC Cuts at: 0 109 962 1125 Size: 109 853 163 Fragments arranged by size: 853 163 109 SmaI CCC'GGG Cuts at: 0 920 1125 Size: 920 205 StyI C'CwwG_G Cuts at: 0 335 1125 Size: 335 790 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 317 331 493 571 1125 Size: 317 14 162 78 554 Fragments arranged by size: 554 317 162 78 14 TatI w'GTAC_w Cuts at: 0 1007 1125 Size: 1007 118 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 631 663 1125 Size: 631 32 462 Fragments arranged by size: 631 462 32 Tth111I GACn'n_nGTC Cuts at: 0 472 1125 Size: 472 653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 181 528 1125 Size: 181 347 597 Fragments arranged by size: 597 347 181 XmaI C'CCGG_G Cuts at: 0 918 1125 Size: 918 207 Enzymes that do cut: AcuI AloI ApaI AvaI BanI BanII BbeI BglI BglII Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI CspCI DraIII EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I KasI MscI MslI NarI NspI PpiI PpuMI PspOMI SapI ScaI SfcI SfiI SfoI SmaI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmtI BplI Bpu10I BpuEI BsgI BsiWI BsmI BspMI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I BtsI ClaI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmnI ZraI