##################################
# NO CUT ENZYMES (  220 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FseI        
FspI        HaeII       HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MboII       MfeI        MflI        MluI        MroI        
MseI        MslI        MspA1I      MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AcyI           50  GGCGTC               G[GA]^CG[CT]C
AluI          102  AGCT                 AG^CT
BalI           44  TGGCCA               TGG^CCA
BbvI          100  GCAGC                GCAGC(8/12)
BclI          126  TGATCA               T^GATCA
BsaHI          50  GGCGTC               G[GA]^CG[CT]C
BseRI         187  GAGGAG               GAGGAG(10/8)
BsmI           76  GCATTC               GAATGC(1/-1)
BspHI         173  TCATGA               T^CATGA
BstF5I         29  GGATG                GGATG(2/0)
DpnII         127  GATC                 ^GATC
EaeI           44  TGGCCA               [CT]^GGCC[GA]
FbaI          126  TGATCA               T^GATCA
Fnu4HI        100  GCAGC                GC^[ACGT]GC
FokI           29  GGATG                GGATG(9/13)
HgaI           51  GCGTC                GACGC(5/10)
Hin1I          50  GGCGTC               G[GA]^CG[CT]C
ItaI          100  GCAGC                GC^[ACGT]GC
MboI          127  GATC                 ^GATC
MluNI          44  TGGCCA               TGG^CCA
MscI           44  TGGCCA               TGG^CCA
NdeI           64  CATATG               CA^TATG
NdeII         127  GATC                 ^GATC
NlaIII        174  CATG                 CATG^
NlaIV           3  GGCTCC               GG[ACGT]^[ACGT]CC
RcaI          173  TCATGA               T^CATGA
Sau3AI        127  GATC                 ^GATC
SfaNI          30  GATGC                GCATC(5/9)
TseI          100  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BssKI          47  CCAGG                ^CC[ACGT]GG
               90  CCTGG               
BstNI          47  CCAGG                CC^[AT]GG
               90  CCTGG               
EcoRII         47  CCAGG                ^CC[AT]GG
               90  CCTGG               
HaeIII         37  GGCC                 GG^CC
               45  GGCC                
MvaI           47  CCAGG                CC^[AT]GG
               90  CCTGG               
ScrFI          47  CCAGG                CC^[ACGT]GG
               90  CCTGG               
SfcI           71  CTGTAG               C^T[GA][CT]AG
              156  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

BsaJI       MnlI