################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpmI BsaAI BsaBI BsaI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HinfI HpaI HphI KpnI MamI MfeI MluI MluNI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 718 GACGTC GACGT^C AccIII 333 TCCGGA T^CCGGA AlwI 503 GGATC GGATC(4/5) AlwNI 676 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 379 GGGCCC GGGCC^C BbeI 559 GGCGCC GGCGC^C BbsI 412 GTCTTC GAAGAC(2/6) BcnI 747 CCGGG CC^[GC]GG BfaI 437 CTAG C^TAG BglI 152 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 57 GCTGAGC GC^T[ACGT]AGC Bpu1102I 57 GCTGAGC GC^T[ACGT]AGC BpuAI 412 GTCTTC GAAGAC(2/6) BsaWI 333 TCCGGA [AT]^CCGG[AT] BseAI 333 TCCGGA T^CCGGA BseRI 621 CTCCTC GAGGAG(10/8) BsmAI 283 GTCTC GTCTC(1/5) BsmBI 282 CGTCTC CGTCTC(1/5) BspEI 333 TCCGGA T^CCGGA BssHII 101 GCGCGC G^CGCGC BssSI 730 CTCGTG CACGAG(-5/-1) BstYI 503 GGATCT [GA]^GATC[CT] CelII 57 GCTGAGC GC^T[ACGT]AGC DdeI 58 CTGAG C^T[ACGT]AG EcoNI 318 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 83 CCAAGG C^C[AT][AT]GG HgaI 410 GCGTC GACGC(5/10) HindIII 204 AAGCTT A^AGCTT KasI 559 GGCGCC G^GCGCC KspI 23 CCGCGG CCGC^GG MaeI 437 CTAG C^TAG MaeII 719 ACGT A^CGT MaeIII 667 GTCAC ^GT[ACGT]AC MflI 503 GGATCT [GA]^GATC[CT] MroI 333 TCCGGA T^CCGGA MseI 551 TTAA T^TAA NaeI 825 GCCGGC GCC^GGC NarI 559 GGCGCC GG^CGCC NciI 747 CCGGG CC^[GC]GG NgoMIV 825 GCCGGC G^CCGGC PstI 256 CTGCAG CTGCA^G PvuII 55 CAGCTG CAG^CTG SacII 23 CCGCGG CCGC^GG SapI 369 GCTCTTC GCTCTTC(1/4) ScaI 212 AGTACT AGT^ACT SfcI 256 CTGCAG C^T[GA][CT]AG SfoI 559 GGCGCC GGC^GCC StyI 83 CCAAGG C^C[AT][AT]GG TaqI 233 TCGA T^CGA Tru9I 551 TTAA T^TAA Tsp45I 667 GTCAC ^GT[GC]AC TthHB8I 233 TCGA T^CGA XhoII 503 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Aor51HI 338 AGCGCT AGC^GCT 431 AGCGCT Asp700I 642 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 645 GAAGTTCTTC AvaI 403 CTCGGG C^[CT]CG[GA]G 511 CCCGAG BanI 559 GGCGCC G^G[CT][GA]CC 588 GGTGCC BanII 223 GGGCTC G[GA]GC[CT]^C 379 GGGCCC BsiEI 68 CGGCCG CG[GA][CT]^CG 159 CGGCCG BsoBI 403 CTCGGG C^[CT]CG[GA]G 511 CCCGAG BsrFI 425 ACCGGC [GA]^CCGG[CT] 825 GCCGGC BstF5I 300 CATCC GGATG(2/0) 690 CATCC Cfr10I 425 ACCGGC [GA]^CCGG[CT] 825 GCCGGC DpnII 351 GATC ^GATC 504 GATC EagI 68 CGGCCG C^GGCCG 159 CGGCCG EarI 370 CTCTTC CTCTTC(1/4) 612 CTCTTC EclXI 68 CGGCCG C^GGCCG 159 CGGCCG Eco47III 338 AGCGCT AGC^GCT 431 AGCGCT Eco52I 68 CGGCCG C^GGCCG 159 CGGCCG FokI 300 CATCC GGATG(9/13) 690 CATCC Ksp632I 370 CTCTTC CTCTTC(1/4) 612 CTCTTC MboI 351 GATC ^GATC 504 GATC MslI 441 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 447 CATGACAGTG NdeII 351 GATC ^GATC 504 GATC Sau3AI 351 GATC ^GATC 504 GATC SfaNI 182 GCATC GCATC(5/9) 299 GCATC XcmI 446 CCATGACAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 593 CCATCTTCCCCGTGG XmnI 642 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 645 GAAGTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BbvI BmyI BsaHI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV PpuMI RsaI Sau96I ScrFI TseI TspRI