Restriction map for pFN21AE6244
 (Circular) (Six-base) MAPSORT of: pFN21AE6244.seq  Check: 2523  from: 1  to: 5539



pFN21AE6244  5539 bp

 With 182 enzymes: SgfI * 

                        March 18, 2015 14:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2671    2753    2671 
   Size:         82    5457
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2727    3349     278 
   Size:         53      83     186     936    1191     622    2468

  Fragments arranged by size: 

                2468    1191     936     622     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2882    1937 
   Size:        945    4594
 
Acc65I G'GTAC_C

Cuts at:   2860    2860 
   Size:       5539
 
AclI AA'CG_TT

Cuts at:   1563    3666    4039    1563 
   Size:       2103     373    3063

  Fragments arranged by size: 

                3063    2103     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2646    3602    4986    5254    1632 
   Size:       1014     956    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1014     956     268
 
AfeI AGC'GCT

Cuts at:   2345    2438    2345 
   Size:         93    5446
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5342
 
AflIII A'CryG_T

Cuts at:   1203    4438    5415    1203 
   Size:       3235     977    1327

  Fragments arranged by size: 

                3235    1327     977
 
AgeI A'CCGG_T

Cuts at:   4370    4370 
   Size:       5539
 
AhdI GACnn_n'nnGTC

Cuts at:   4268    4268 
   Size:       5539
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5539
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5539
 
AlwNI CAG_nnn'CTG

Cuts at:   2686    3282    4854    2686 
   Size:        596    1572    3371

  Fragments arranged by size: 

                3371    1572     596
 
ApaI G_GGCC'C

Cuts at:   2388    2388 
   Size:       5539
 
ApaLI G'TGCA_C

Cuts at:   3596    4752    3596 
   Size:       1156    4383
 
ApoI r'AATT_y

Cuts at:   1493    2848    3074    3226    5335    1493 
   Size:       1355     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1355     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4093     160 
   Size:        872    3061    1606

  Fragments arranged by size: 

                3061    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5539
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2408    2516    2864    1870 
   Size:         73     465     108     348    4545

  Fragments arranged by size: 

                4545     465     348     108      73
 
BamHI G'GATC_C

Cuts at:   2869    3236    2869 
   Size:        367    5172
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2564    2593    2860    4315     618 
   Size:        325     217     627     777      29     267    1455    1842

  Fragments arranged by size: 

                1842    1455     777     627     325     267     217      29
            
 
BanII G_rGCy'C

Cuts at:   1391    2232    2388    2858    1391 
   Size:        841     156     470    4072

  Fragments arranged by size: 

                4072     841     470     156
 
BbeI G_GCGC'C

Cuts at:   2568    2568 
   Size:       5539
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2410     928 
   Size:        971     511    4057

  Fragments arranged by size: 

                4057     971     511
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3730    3764    1903 
   Size:         34    1793      34    3678

  Fragments arranged by size: 

                3678    1793      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3431    4641    3431 
   Size:       1210    4329
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5539
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2163    4150     136 
   Size:        107     122      71     631     605     491    1987    1525

  Fragments arranged by size: 

                1987    1525     631     605     491     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5534    5534 
   Size:       5539
 
BlpI GC'TnA_GC

Cuts at:   2063    2956    2063 
   Size:        893    4646
 
Bme1580I G_kGCm'C

Cuts at:   1790    2388    2598    3600    4756    1790 
   Size:        598     210    1002    1156    2573

  Fragments arranged by size: 

                2573    1156    1002     598     210
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5539
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3270    4228     442 
   Size:        416      53      83     399      24    1853     958    1753

  Fragments arranged by size: 

                1853    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5539
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4199    1239 
   Size:       2960    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3664    4529    4827    3664 
   Size:        865     298    4376

  Fragments arranged by size: 

                4376     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4202     882 
   Size:        617    2703    2219

  Fragments arranged by size: 

                2703    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3330     493 
   Size:        711    2126    2702

  Fragments arranged by size: 

                2702    2126     711
 
BsaBI GATnn'nnATC

Cuts at:   5533    5533 
   Size:       5539
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2415    2565    2724    3346 
   Size:         53      83     186     936     882     150     159     622

Cuts at:   3346    3728    4363     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                1451     936     882     635     622     382     186     159
                 150      83      53
 
BsaWI w'CCGG_w

Cuts at:   2338    3972    4370    4644    4791    5202    2338 
   Size:       1634     398     274     147     411    2675

  Fragments arranged by size: 

                2675    1634     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5314

  Fragments arranged by size: 

                5314     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2617    1186 
   Size:       1431    4108
 
BseYI C'CCAG_C

Cuts at:   4742    5238    4742 
   Size:        496    5043
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2078    2372    2699    2846    1163 
   Size:        573     342     294     327     147    3856

  Fragments arranged by size: 

                3856     573     342     327     294     147
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2076    2167    2989    3750    4778    1540 
   Size:        275     186      75      91     822     761    1028    2301

  Fragments arranged by size: 

                2301    1028     822     761     275     186      91      75
            
 
BsiHKAI G_wGCw'C

Cuts at:   3600    3685    4756    3600 
   Size:         85    1071    4383

  Fragments arranged by size: 

                4383    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3050    3143    3050 
   Size:         93    5446
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2293    2293 
   Size:       5539
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2232    2388    2598    2858    3600    3685    4756 
   Size:        399     442     156     210     260     742      85    1071

Cuts at:   4756    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     442     399     260     210     156
                  85
 
BspEI T'CCGG_A

Cuts at:   2338    5202    2338 
   Size:       2864    2675
 
BspHI T'CATG_A

Cuts at:   3428    5158    3428 
   Size:       1730    3809
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2553    2671    2753    2894     844 
   Size:       1709     118      82     141    3489

  Fragments arranged by size: 

                3489    1709     141     118      82
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3426    1394 
   Size:        429    1603    3507

  Fragments arranged by size: 

                3507    1603     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4034    4208      65 
   Size:       1183    2786     174    1396

  Fragments arranged by size: 

                2786    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2430    2830    4183    4370    1700 
   Size:        110     620     400    1353     187    2869

  Fragments arranged by size: 

                2869    1353     620     400     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5539
 
BssHII G'CGCG_C

Cuts at:   1925    2106    1925 
   Size:        181    5358
 
BssSI C'ACGA_G

Cuts at:   2735    3599    4611    2735 
   Size:        864    1012    3663

  Fragments arranged by size: 

                3663    1012     864
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5539
 
BstBI TT'CG_AA

Cuts at:   4343    4343 
   Size:       5539
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5539
 
BstYI r'GATC_y

Cuts at:   1972    2508    2869    3236    3621    3638    5090    5176    5188 
   Size:        536     361     367     385      17    1452      86      12

Cuts at:   5188    5534    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     536     385     367     361     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2028    2130    2158    2388    2607     513 
   Size:        552     939      24     102      28     230     219    3445

  Fragments arranged by size: 

                3445     939     552     230     219     102      28      24
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4800

  Fragments arranged by size: 

                4800     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2445    2712    2808    3055    3849    3869    2445 
   Size:        267      96     247     794      20    4115

  Fragments arranged by size: 

                4115     794     267     247      96      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5539
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5504
 
DraI TTT'AAA

Cuts at:   2843    2843 
   Size:       5539
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4546     809 
   Size:        507    3230    1802

  Fragments arranged by size: 

                3230    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2015    2025    2073 
   Size:         54    1425      50     275      18     185      10      48

Cuts at:   2073    2164    2463    2986    3875    5504       8 
   Size:         91     299     523     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     523     299     275     185      91
                  54      50      48      43      18      10
 
EagI C'GGCC_G

Cuts at:   1537    1812    2073    2164    2986    1537 
   Size:        275     261      91     822    4090

  Fragments arranged by size: 

                4090     822     275     261      91
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2381    2623    3469    1353 
   Size:       1028     242     846    3423

  Fragments arranged by size: 

                3423    1028     846     242
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2116    2735    4126    4500    4646     387 
   Size:       1064     138     527     619    1391     374     146    1280

  Fragments arranged by size: 

                1391    1280    1064     619     527     374     146     138
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2646    3602    4199    4986    5254    1239 
   Size:        393    1014     956     597     787     268    1524

  Fragments arranged by size: 

                1524    1014     956     787     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2327    2327 
   Size:       5539
 
EcoO109I rG'GnC_Cy

Cuts at:   2248    2332    2384    2542    2248 
   Size:         84      52     158    5245

  Fragments arranged by size: 

                5245     158      84      52
 
EcoRI G'AATT_C

Cuts at:   2848    3226    2848 
   Size:        378    5161
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2584    2671    2584 
   Size:         87    5452
 
FspI TGC'GCA

Cuts at:   4045    4045 
   Size:       5539
 
HaeII r_GCGC'y

Cuts at:   1678    2036    2347    2440    2568    4686    1678 
   Size:        358     311      93     128    2118    2531

  Fragments arranged by size: 

                2531    2118     358     311     128      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4180    4212    4254    4286    1373 
   Size:         32     135      32    2608      32      42      32    2626

  Fragments arranged by size: 

                2626    2608     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2883    3129     669 
   Size:        988     281     945     246    3079

  Fragments arranged by size: 

                3079     988     945     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2209     748 
   Size:       1461    4078
 
HpaI GTT'AAC

Cuts at:   3129    3129 
   Size:       5539
 
KasI G'GCGC_C

Cuts at:   2564    2564 
   Size:       5539
 
KpnI G_GTAC'C

Cuts at:   2864    2864 
   Size:       5539
 
MfeI C'AATT_G

Cuts at:   3138    3138 
   Size:       5539
 
MluI A'CGCG_T

Cuts at:   5415    5415 
   Size:       5539
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2316    4653    4837    1305 
   Size:       1011    2337     184    2007

  Fragments arranged by size: 

                2337    2007    1011     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3717

  Fragments arranged by size: 

                3717    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2450    2456    3497    3856    4015     518 
   Size:        648    1284       6    1041     359     159    2042

  Fragments arranged by size: 

                2042    1284    1041     648     359     159       6
 
MspA1I CmG'CkG

Cuts at:   1606    2030    2062    2706    2955    3632    4780    5025    1606 
   Size:        424      32     644     249     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     644     424     249     245      32
            
 
NaeI GCC'GGC

Cuts at:   2832    2832 
   Size:       5539
 
NarI GG'CG_CC

Cuts at:   2565    2565 
   Size:       5539
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4048

  Fragments arranged by size: 

                4048     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5539
 
NgoMIV G'CCGG_C

Cuts at:   2830    2830 
   Size:       5539
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5539
 
NotI GC'GGCC_GC

Cuts at:   2986    2986 
   Size:       5539
 
NspI r_CATG'y

Cuts at:   1132    1229    2897    4442    1132 
   Size:         97    1668    1545    2229

  Fragments arranged by size: 

                2229    1668    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5539
 
PciI A'CATG_T

Cuts at:   4438    4438 
   Size:       5539
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5539
 
PfoI T'CCnGG_A

Cuts at:   2307    2307 
   Size:       5539
 
PmeI GTTT'AAAC

Cuts at:   2843    2843 
   Size:       5539
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3608    3640    5121    5153    3608 
   Size:         32    1481      32    3994

  Fragments arranged by size: 

                3994    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2248    2332    2542    2248 
   Size:         84     210    5245

  Fragments arranged by size: 

                5245     210      84
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5539
 
PsiI TTA'TAA

Cuts at:   3109    3109 
   Size:       5539
 
PspOMI G'GGCC_C

Cuts at:   2384    2384 
   Size:       5539
 
PstI C_TGCA'G

Cuts at:    830    2265    2891     830 
   Size:       1435     626    3478

  Fragments arranged by size: 

                3478    1435     626
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5539
 
PvuII CAG'CTG

Cuts at:   2062    2062 
   Size:       5539
 
SacII CC_GC'GG

Cuts at:   2031    2031 
   Size:       5539
 
SalI G'TCGA_C

Cuts at:   1936    2881    1936 
   Size:        945    4594
 
SanDI GG'GwC_CC

Cuts at:   2248    2248 
   Size:       5539
 
SapI GCTCTTCn'nnn_

Cuts at:   2381    2381 
   Size:       5539
 
SbfI CC_TGCA'GG

Cuts at:   2891    2891 
   Size:       5539
 
ScaI AGT'ACT

Cuts at:   2219    3787    5499    2219 
   Size:       1568    1712    2259

  Fragments arranged by size: 

                2259    1712    1568
 
SfcI C'TryA_G

Cuts at:    826    1045    2261    2887    4022    4703    4894     826 
   Size:        219    1216     626    1135     681     191    1471

  Fragments arranged by size: 

                1471    1216    1135     681     626     219     191
 
SfoI GGC'GCC

Cuts at:   2566    2566 
   Size:       5539
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5539
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5539
 
SmaI CCC'GGG

Cuts at:   1872    2866    1872 
   Size:        994    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3643    4544    4806     820 
   Size:        197     926    1700     901     262    1553

  Fragments arranged by size: 

                1700    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3330     493 
   Size:       2837    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5539
 
SphI G_CATG'C

Cuts at:   1132    2897    1132 
   Size:       1765    3774
 
SspI AAT'ATT

Cuts at:      5      52    3463       5 
   Size:         47    3411    2081

  Fragments arranged by size: 

                3411    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5539
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2088    2978     513 
   Size:        552     939      84     890    3074

  Fragments arranged by size: 

                3074     939     890     552      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3566    3583    3736    3921    4366     325 
   Size:       1255    1986      17     153     185     445    1498

  Fragments arranged by size: 

                1986    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2100    2217    2613 
   Size:        274      80      33      51    1443     123     117     396

Cuts at:   2613    3785    5497      96 
   Size:       1172    1712     138

  Fragments arranged by size: 

                1712    1443    1172     396     274     138     123     117
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5499    5531    5499 
   Size:         32    5507
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5539
 
XbaI T'CTAG_A

Cuts at:   2875    2875 
   Size:       5539
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2458    2605    2458 
   Size:        147    5392
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5539
 
XmaI C'CCGG_G

Cuts at:   1870    2864    1870 
   Size:        994    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2651    2654    3668    2651 
   Size:          3    1014    4522

  Fragments arranged by size: 

                4522    1014       3
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2725    3347     276 
   Size:         53      83     186     936    1191     622    2468

  Fragments arranged by size: 

                2468    1191     936     622     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PstI     PvuI    PvuII    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII     BaeI    BbvCI    BfrBI     BplI   Bpu10I
    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoRV     FseI
    FspAI     NruI     NsiI     PacI     PmlI    PspXI     PsrI    RsrII
     SacI    SexAI     SfiI     SrfI     SwaI
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