(Linear) (Six-base) MAPSORT of: KIEE6244.seq Check: 3572 from: 1 to: 834 KIEE6244.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 666 748 834 Size: 666 82 86 Fragments arranged by size: 666 86 82 AatII G_ACGT'C Cuts at: 0 722 834 Size: 722 112 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 641 834 Size: 641 193 AfeI AGC'GCT Cuts at: 0 340 433 834 Size: 340 93 401 Fragments arranged by size: 401 340 93 AlwNI CAG_nnn'CTG Cuts at: 0 681 834 Size: 681 153 ApaI G_GGCC'C Cuts at: 0 383 834 Size: 383 451 AvaI C'yCGr_G Cuts at: 0 403 511 834 Size: 403 108 323 Fragments arranged by size: 403 323 108 BanI G'GyrC_C Cuts at: 0 559 588 834 Size: 559 29 246 Fragments arranged by size: 559 246 29 BanII G_rGCy'C Cuts at: 0 227 383 834 Size: 227 156 451 Fragments arranged by size: 451 227 156 BbeI G_GCGC'C Cuts at: 0 563 834 Size: 563 271 BbsI GAAGACnn'nnnn_ Cuts at: 0 405 834 Size: 405 429 BglI GCCn_nnn'nGGC Cuts at: 0 158 834 Size: 158 676 BlpI GC'TnA_GC Cuts at: 0 58 834 Size: 58 776 Bme1580I G_kGCm'C Cuts at: 0 383 593 834 Size: 383 210 241 Fragments arranged by size: 383 241 210 BsaHI Gr'CG_yC Cuts at: 0 410 560 719 834 Size: 410 150 159 115 Fragments arranged by size: 410 159 150 115 BsaWI w'CCGG_w Cuts at: 0 333 834 Size: 333 501 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 612 834 Size: 612 222 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 367 694 834 Size: 73 294 327 140 Fragments arranged by size: 327 294 140 73 BsiEI CG_ry'CG Cuts at: 0 71 162 834 Size: 71 91 672 Fragments arranged by size: 672 91 71 BsmBI CGTCTCn'nnnn_ Cuts at: 0 288 834 Size: 288 546 Bsp1286I G_dGCh'C Cuts at: 0 227 383 593 834 Size: 227 156 210 241 Fragments arranged by size: 241 227 210 156 BspEI T'CCGG_A Cuts at: 0 333 834 Size: 333 501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 548 666 748 834 Size: 548 118 82 86 Fragments arranged by size: 548 118 86 82 BsrFI r'CCGG_y Cuts at: 0 425 825 834 Size: 425 400 9 Fragments arranged by size: 425 400 9 BssHII G'CGCG_C Cuts at: 0 101 834 Size: 101 733 BssSI C'ACGA_G Cuts at: 0 730 834 Size: 730 104 BstYI r'GATC_y Cuts at: 0 503 834 Size: 503 331 BtgI C'CryG_G Cuts at: 0 23 125 153 383 602 834 Size: 23 102 28 230 219 232 Fragments arranged by size: 232 230 219 102 28 23 BtsI GCAGTG_nn' Cuts at: 0 440 707 803 834 Size: 440 267 96 31 Fragments arranged by size: 440 267 96 31 EaeI y'GGCC_r Cuts at: 0 10 20 68 159 458 834 Size: 10 10 48 91 299 376 Fragments arranged by size: 376 299 91 48 10 10 EagI C'GGCC_G Cuts at: 0 68 159 834 Size: 68 91 675 Fragments arranged by size: 675 91 68 EarI CTCTTCn'nnn_ Cuts at: 0 376 618 834 Size: 376 242 216 Fragments arranged by size: 376 242 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 111 730 834 Size: 111 619 104 Fragments arranged by size: 619 111 104 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 641 834 Size: 641 193 EcoNI CCTnn'n_nnAGG Cuts at: 0 322 834 Size: 322 512 EcoO109I rG'GnC_Cy Cuts at: 0 243 327 379 537 834 Size: 243 84 52 158 297 Fragments arranged by size: 297 243 158 84 52 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 579 666 834 Size: 579 87 168 Fragments arranged by size: 579 168 87 HaeII r_GCGC'y Cuts at: 0 31 342 435 563 834 Size: 31 311 93 128 271 Fragments arranged by size: 311 271 128 93 31 HindIII A'AGCT_T Cuts at: 0 204 834 Size: 204 630 KasI G'GCGC_C Cuts at: 0 559 834 Size: 559 275 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 311 834 Size: 311 523 MslI CAynn'nnrTG Cuts at: 0 445 451 834 Size: 445 6 383 Fragments arranged by size: 445 383 6 MspA1I CmG'CkG Cuts at: 0 25 57 701 834 Size: 25 32 644 133 Fragments arranged by size: 644 133 32 25 NaeI GCC'GGC Cuts at: 0 827 834 Size: 827 7 NarI GG'CG_CC Cuts at: 0 560 834 Size: 560 274 NgoMIV G'CCGG_C Cuts at: 0 825 834 Size: 825 9 PfoI T'CCnGG_A Cuts at: 0 302 834 Size: 302 532 PpuMI rG'GwC_Cy Cuts at: 0 243 327 537 834 Size: 243 84 210 297 Fragments arranged by size: 297 243 210 84 PspOMI G'GGCC_C Cuts at: 0 379 834 Size: 379 455 PstI C_TGCA'G Cuts at: 0 260 834 Size: 260 574 PvuII CAG'CTG Cuts at: 0 57 834 Size: 57 777 SacII CC_GC'GG Cuts at: 0 26 834 Size: 26 808 SanDI GG'GwC_CC Cuts at: 0 243 834 Size: 243 591 SapI GCTCTTCn'nnn_ Cuts at: 0 376 834 Size: 376 458 ScaI AGT'ACT Cuts at: 0 214 834 Size: 214 620 SfcI C'TryA_G Cuts at: 0 256 834 Size: 256 578 SfoI GGC'GCC Cuts at: 0 561 834 Size: 561 273 StyI C'CwwG_G Cuts at: 0 83 834 Size: 83 751 TatI w'GTAC_w Cuts at: 0 95 212 608 834 Size: 95 117 396 226 Fragments arranged by size: 396 226 117 95 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 453 600 834 Size: 453 147 234 Fragments arranged by size: 453 234 147 XmnI GAAnn'nnTTC Cuts at: 0 646 649 834 Size: 646 3 185 Fragments arranged by size: 646 185 3 ZraI GAC'GTC Cuts at: 0 720 834 Size: 720 114 Enzymes that do cut: AarI AatII AcuI AfeI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BsaHI BsaWI BseRI BsgI BsiEI BsmBI Bsp1286I BspEI BspMI BsrFI BssHII BssSI BstYI BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII HindIII KasI MmeI MslI MspA1I NaeI NarI NgoMIV PfoI PpuMI PspOMI PstI PvuII SacII SanDI SapI ScaI SfcI SfoI StyI TatI XcmI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsiHKAI BsiWI BsmI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI