Restriction map for pFN21AE6244
 (Linear) (Six-base) MAPSORT of: KIEE6244.seq  Check: 3572  from: 1  to: 834



KIEE6244.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     666     748     834 
   Size:        666      82      86

  Fragments arranged by size: 

                 666      86      82
 
AatII G_ACGT'C

Cuts at:      0     722     834 
   Size:        722     112
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     834 
   Size:        641     193
 
AfeI AGC'GCT

Cuts at:      0     340     433     834 
   Size:        340      93     401

  Fragments arranged by size: 

                 401     340      93
 
AlwNI CAG_nnn'CTG

Cuts at:      0     681     834 
   Size:        681     153
 
ApaI G_GGCC'C

Cuts at:      0     383     834 
   Size:        383     451
 
AvaI C'yCGr_G

Cuts at:      0     403     511     834 
   Size:        403     108     323

  Fragments arranged by size: 

                 403     323     108
 
BanI G'GyrC_C

Cuts at:      0     559     588     834 
   Size:        559      29     246

  Fragments arranged by size: 

                 559     246      29
 
BanII G_rGCy'C

Cuts at:      0     227     383     834 
   Size:        227     156     451

  Fragments arranged by size: 

                 451     227     156
 
BbeI G_GCGC'C

Cuts at:      0     563     834 
   Size:        563     271
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     405     834 
   Size:        405     429
 
BglI GCCn_nnn'nGGC

Cuts at:      0     158     834 
   Size:        158     676
 
BlpI GC'TnA_GC

Cuts at:      0      58     834 
   Size:         58     776
 
Bme1580I G_kGCm'C

Cuts at:      0     383     593     834 
   Size:        383     210     241

  Fragments arranged by size: 

                 383     241     210
 
BsaHI Gr'CG_yC

Cuts at:      0     410     560     719     834 
   Size:        410     150     159     115

  Fragments arranged by size: 

                 410     159     150     115
 
BsaWI w'CCGG_w

Cuts at:      0     333     834 
   Size:        333     501
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     612     834 
   Size:        612     222
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     367     694     834 
   Size:         73     294     327     140

  Fragments arranged by size: 

                 327     294     140      73
 
BsiEI CG_ry'CG

Cuts at:      0      71     162     834 
   Size:         71      91     672

  Fragments arranged by size: 

                 672      91      71
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     288     834 
   Size:        288     546
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     383     593     834 
   Size:        227     156     210     241

  Fragments arranged by size: 

                 241     227     210     156
 
BspEI T'CCGG_A

Cuts at:      0     333     834 
   Size:        333     501
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     548     666     748     834 
   Size:        548     118      82      86

  Fragments arranged by size: 

                 548     118      86      82
 
BsrFI r'CCGG_y

Cuts at:      0     425     825     834 
   Size:        425     400       9

  Fragments arranged by size: 

                 425     400       9
 
BssHII G'CGCG_C

Cuts at:      0     101     834 
   Size:        101     733
 
BssSI C'ACGA_G

Cuts at:      0     730     834 
   Size:        730     104
 
BstYI r'GATC_y

Cuts at:      0     503     834 
   Size:        503     331
 
BtgI C'CryG_G

Cuts at:      0      23     125     153     383     602     834 
   Size:         23     102      28     230     219     232

  Fragments arranged by size: 

                 232     230     219     102      28      23
 
BtsI GCAGTG_nn'

Cuts at:      0     440     707     803     834 
   Size:        440     267      96      31

  Fragments arranged by size: 

                 440     267      96      31
 
EaeI y'GGCC_r

Cuts at:      0      10      20      68     159     458     834 
   Size:         10      10      48      91     299     376

  Fragments arranged by size: 

                 376     299      91      48      10      10
 
EagI C'GGCC_G

Cuts at:      0      68     159     834 
   Size:         68      91     675

  Fragments arranged by size: 

                 675      91      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     376     618     834 
   Size:        376     242     216

  Fragments arranged by size: 

                 376     242     216
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     111     730     834 
   Size:        111     619     104

  Fragments arranged by size: 

                 619     111     104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     834 
   Size:        641     193
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     322     834 
   Size:        322     512
 
EcoO109I rG'GnC_Cy

Cuts at:      0     243     327     379     537     834 
   Size:        243      84      52     158     297

  Fragments arranged by size: 

                 297     243     158      84      52
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     579     666     834 
   Size:        579      87     168

  Fragments arranged by size: 

                 579     168      87
 
HaeII r_GCGC'y

Cuts at:      0      31     342     435     563     834 
   Size:         31     311      93     128     271

  Fragments arranged by size: 

                 311     271     128      93      31
 
HindIII A'AGCT_T

Cuts at:      0     204     834 
   Size:        204     630
 
KasI G'GCGC_C

Cuts at:      0     559     834 
   Size:        559     275
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     834 
   Size:        311     523
 
MslI CAynn'nnrTG

Cuts at:      0     445     451     834 
   Size:        445       6     383

  Fragments arranged by size: 

                 445     383       6
 
MspA1I CmG'CkG

Cuts at:      0      25      57     701     834 
   Size:         25      32     644     133

  Fragments arranged by size: 

                 644     133      32      25
 
NaeI GCC'GGC

Cuts at:      0     827     834 
   Size:        827       7
 
NarI GG'CG_CC

Cuts at:      0     560     834 
   Size:        560     274
 
NgoMIV G'CCGG_C

Cuts at:      0     825     834 
   Size:        825       9
 
PfoI T'CCnGG_A

Cuts at:      0     302     834 
   Size:        302     532
 
PpuMI rG'GwC_Cy

Cuts at:      0     243     327     537     834 
   Size:        243      84     210     297

  Fragments arranged by size: 

                 297     243     210      84
 
PspOMI G'GGCC_C

Cuts at:      0     379     834 
   Size:        379     455
 
PstI C_TGCA'G

Cuts at:      0     260     834 
   Size:        260     574
 
PvuII CAG'CTG

Cuts at:      0      57     834 
   Size:         57     777
 
SacII CC_GC'GG

Cuts at:      0      26     834 
   Size:         26     808
 
SanDI GG'GwC_CC

Cuts at:      0     243     834 
   Size:        243     591
 
SapI GCTCTTCn'nnn_

Cuts at:      0     376     834 
   Size:        376     458
 
ScaI AGT'ACT

Cuts at:      0     214     834 
   Size:        214     620
 
SfcI C'TryA_G

Cuts at:      0     256     834 
   Size:        256     578
 
SfoI GGC'GCC

Cuts at:      0     561     834 
   Size:        561     273
 
StyI C'CwwG_G

Cuts at:      0      83     834 
   Size:         83     751
 
TatI w'GTAC_w

Cuts at:      0      95     212     608     834 
   Size:         95     117     396     226

  Fragments arranged by size: 

                 396     226     117      95
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     453     600     834 
   Size:        453     147     234

  Fragments arranged by size: 

                 453     234     147
 
XmnI GAAnn'nnTTC

Cuts at:      0     646     649     834 
   Size:        646       3     185

  Fragments arranged by size: 

                 646     185       3
 
ZraI GAC'GTC

Cuts at:      0     720     834 
   Size:        720     114
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI     BbsI     BglI     BlpI Bme1580I    BsaHI    BsaWI
    BseRI     BsgI    BsiEI    BsmBI Bsp1286I    BspEI    BspMI    BsrFI
   BssHII    BssSI    BstYI     BtgI     BtsI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII  HindIII     KasI
     MmeI     MslI   MspA1I     NaeI     NarI   NgoMIV     PfoI    PpuMI
   PspOMI     PstI    PvuII    SacII    SanDI     SapI     ScaI     SfcI
     SfoI     StyI     TatI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BseYI  BsiHKAI    BsiWI     BsmI    BspHI
    BsrBI    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII     HpaI
     KpnI     MfeI     MluI     MscI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
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