Restriction map for
pFN21AE0419
##################################
# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanII BbeI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MboI MboII MfeI
MflI MluI MroI MseI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 147 GTAC GT^AC
Alw44I 311 GTGCAC G^TGCAC
ApaLI 311 GTGCAC G^TGCAC
AvaI 50 CCCGAG C^[CT]CG[GA]G
BpmI 457 CTCCAG CTGGAG(16/14)
BsaI 45 GGTCTC GGTCTC(1/5)
BsmFI 477 GTCCC GGGAC(10/14)
BsoBI 50 CCCGAG C^[CT]CG[GA]G
BspMI 42 GCAGGT ACCTGC(4/8)
BsrBI 222 CCGCTC CCGCTC(-3/-3)
BssSI 420 CTCGTG CACGAG(-5/-1)
BstEII 63 GGTGACC G^GT[ACGT]ACC
BstF5I 141 CATCC GGATG(2/0)
BstPI 63 GGTGACC G^GT[ACGT]ACC
BstXI 318 CCACCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 260 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 417 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 373 GACAAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 464 CCACGG C^C[GA][CT]GG
EcoNI 453 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 260 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 63 GGTGACC G^GT[ACGT]ACC
FokI 141 CATCC GGATG(9/13)
PpuMI 260 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 147 GTAC GT^AC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 305 ACACGT A^C[GA][CT]GT
307 ACGTGT
AvaII 90 GGACC G^G[AT]CC
261 GGTCC
BalI 8 TGGCCA TGG^CCA
327 TGGCCA
BanI 204 GGTGCC G^G[CT][GA]CC
298 GGCACC
BbrPI 181 CACGTG CAC^GTG
306 CACGTG
BfaI 85 CTAG C^TAG
284 CTAG
BsaAI 181 CACGTG [CT]AC^GT[GA]
306 CACGTG
BsaJI 51 CCGAGG C^C[ACGT][ACGT]GG
464 CCACGG
BseRI 345 CTCCTC GAGGAG(10/8)
454 CTCCTC
BsrI 378 ACTGG ACTGG(1/-1)
144 CCAGT
BssKI 6 CCTGG ^CC[ACGT]GG
459 CCAGG
BstNI 6 CCTGG CC^[AT]GG
459 CCAGG
Cfr13I 90 GGACC G^G[ACGT]CC
261 GGTCC
EaeI 8 TGGCCA [CT]^GGCC[GA]
327 TGGCCA
EcoRII 6 CCTGG ^CC[AT]GG
459 CCAGG
MaeI 85 CTAG C^TAG
284 CTAG
MaeIII 64 GTGAC ^GT[ACGT]AC
358 GTCAC
MluNI 8 TGGCCA TGG^CCA
327 TGGCCA
MscI 8 TGGCCA TGG^CCA
327 TGGCCA
MvaI 6 CCTGG CC^[AT]GG
459 CCAGG
MwoI 426 GCTGCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
432 GCTGCTCCTGC
NlaIV 204 GGTGCC GG[ACGT]^[ACGT]CC
298 GGCACC
PmaCI 181 CACGTG CAC^GTG
306 CACGTG
PmlI 181 CACGTG CAC^GTG
306 CACGTG
Sau96I 90 GGACC G^G[ACGT]CC
261 GGTCC
ScrFI 6 CCTGG CC^[ACGT]GG
459 CCAGG
SfaNI 17 GCATC GCATC(5/9)
395 GCATC
Tsp45I 64 GTGAC ^GT[GC]AC
358 GTCAC
TspRI 195 CACTG CA[GC]TG[ACGT][ACGT]^
362 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AspHI BbvI BmyI BsiHKAI
BsiYI BslI BsmAI Bsp1286I Cac8I
DdeI Fnu4HI HaeIII HphI ItaI
MaeII MnlI NlaIII TseI