(Circular) (Six-base) MAPSORT of: pFN21AE0419.seq Check: 4007 from: 1 to: 5188 pFN21AE0419 5188 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2998 278 Size: 53 83 186 936 1462 2468 Fragments arranged by size: 2468 1462 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2531 1937 Size: 594 4594 Acc65I G'GTAC_C Cuts at: 2509 2509 Size: 5188 AclI AA'CG_TT Cuts at: 1563 3315 3688 1563 Size: 1752 373 3063 Fragments arranged by size: 3063 1752 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3251 4635 4903 1632 Size: 1619 1384 268 1917 Fragments arranged by size: 1917 1619 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4991 AflIII A'CryG_T Cuts at: 1203 2310 2312 4087 5064 1203 Size: 1107 2 1775 977 1327 Fragments arranged by size: 1775 1327 1107 977 2 AgeI A'CCGG_T Cuts at: 4019 4019 Size: 5188 AhdI GACnn_n'nnGTC Cuts at: 3917 3917 Size: 5188 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5188 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2423 1184 Size: 1239 3949 AlwNI CAG_nnn'CTG Cuts at: 2931 4503 2931 Size: 1572 3616 ApaLI G'TGCA_C Cuts at: 2316 3245 4401 2316 Size: 929 1156 3103 Fragments arranged by size: 3103 1156 929 ApoI r'AATT_y Cuts at: 1493 2497 2723 2875 4984 1493 Size: 1004 226 152 2109 1697 Fragments arranged by size: 2109 1697 1004 226 152 AseI AT'TA_AT Cuts at: 160 1032 3742 160 Size: 872 2710 1606 Fragments arranged by size: 2710 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5188 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2513 1870 Size: 73 112 458 4545 Fragments arranged by size: 4545 458 112 73 BamHI G'GATC_C Cuts at: 2518 2885 2518 Size: 367 4821 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2209 2303 2509 3964 618 Size: 325 217 627 422 94 206 1455 1842 Fragments arranged by size: 1842 1455 627 422 325 217 206 94 BanII G_rGCy'C Cuts at: 1391 2507 1391 Size: 1116 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3379 3413 1903 Size: 34 1442 34 3678 Fragments arranged by size: 3678 1442 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3080 4290 3080 Size: 1210 3978 BclI T'GATC_A Cuts at: 1548 1548 Size: 5188 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3799 136 Size: 107 122 71 631 605 2127 1525 Fragments arranged by size: 2127 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5183 5183 Size: 5188 BlpI GC'TnA_GC Cuts at: 2605 2605 Size: 5188 Bme1580I G_kGCm'C Cuts at: 1790 2306 2320 3249 4405 1790 Size: 516 14 929 1156 2573 Fragments arranged by size: 2573 1156 929 516 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5188 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2919 3877 442 Size: 416 53 83 399 24 1502 958 1753 Fragments arranged by size: 1753 1502 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5188 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2447 3848 1239 Size: 1208 1401 2579 Fragments arranged by size: 2579 1401 1208 Bpu10I CC'TnA_GC Cuts at: 2214 2214 Size: 5188 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3313 4178 4476 3313 Size: 865 298 4025 Fragments arranged by size: 4025 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2056 3851 882 Size: 617 557 1795 2219 Fragments arranged by size: 2219 1795 617 557 BsaAI yAC'GTr Cuts at: 493 1204 2188 2313 2979 493 Size: 711 984 125 666 2702 Fragments arranged by size: 2702 984 711 666 125 BsaBI GATnn'nnATC Cuts at: 5182 5182 Size: 5188 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2995 3377 4012 275 Size: 53 83 186 936 1462 382 635 1451 Fragments arranged by size: 1462 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3621 4019 4293 4440 4851 3621 Size: 398 274 147 411 3958 Fragments arranged by size: 3958 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4963 Fragments arranged by size: 4963 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2341 2450 1186 Size: 1155 109 3924 Fragments arranged by size: 3924 1155 109 BseYI C'CCAG_C Cuts at: 4391 4887 4391 Size: 496 4692 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4615 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2638 3399 4427 1540 Size: 275 186 637 761 1028 2301 Fragments arranged by size: 2301 1028 761 637 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2220 2320 2373 3249 3334 4405 2193 Size: 27 100 53 876 85 1071 2976 Fragments arranged by size: 2976 1071 876 100 85 53 27 BsmI GAATG_Cn' Cuts at: 2699 2792 2699 Size: 93 5095 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2220 2306 2320 2373 2507 3249 Size: 399 403 27 86 14 53 134 742 Cuts at: 3249 3334 4405 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 134 86 85 53 27 14 BspEI T'CCGG_A Cuts at: 4851 4851 Size: 5188 BspHI T'CATG_A Cuts at: 3077 4807 3077 Size: 1730 3458 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2038 2543 844 Size: 1194 505 3489 Fragments arranged by size: 3489 1194 505 BsrBI CCG'CTC Cuts at: 1394 1823 2229 3075 1394 Size: 429 406 846 3507 Fragments arranged by size: 3507 846 429 406 BsrDI GCAATG_nn' Cuts at: 65 1248 3683 3857 65 Size: 1183 2435 174 1396 Fragments arranged by size: 2435 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3832 4019 1700 Size: 110 2022 187 2869 Fragments arranged by size: 2869 2022 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5188 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5188 BssSI C'ACGA_G Cuts at: 2425 3248 4260 2425 Size: 823 1012 3353 Fragments arranged by size: 3353 1012 823 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5188 BstBI TT'CG_AA Cuts at: 3992 3992 Size: 5188 BstEII G'GTnAC_C Cuts at: 1184 2068 1184 Size: 884 4304 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2330 2011 Size: 319 4869 BstYI r'GATC_y Cuts at: 1972 2518 2885 3270 3287 4739 4825 4837 5183 Size: 546 367 385 17 1452 86 12 346 Cuts at: 5183 1972 Size: 1977 Fragments arranged by size: 1977 1452 546 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2469 513 Size: 552 1404 3232 Fragments arranged by size: 3232 1404 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2008 487 Size: 683 56 782 3667 Fragments arranged by size: 3667 782 683 56 BtsI GCAGTG_nn' Cuts at: 2704 3498 3518 2704 Size: 794 20 4374 Fragments arranged by size: 4374 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5153 DraI TTT'AAA Cuts at: 2492 2492 Size: 5188 DraIII CAC_nnn'GTG Cuts at: 2427 2427 Size: 5188 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2384 4195 809 Size: 507 1068 1811 1802 Fragments arranged by size: 1811 1802 1068 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2332 2635 Size: 54 1425 50 275 18 183 319 303 Cuts at: 2635 3524 5153 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 319 303 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2635 1537 Size: 275 823 4090 Fragments arranged by size: 4090 823 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3118 1353 Size: 1765 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2269 3775 4149 4295 387 Size: 1064 138 680 1506 374 146 1280 Fragments arranged by size: 1506 1280 1064 680 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2447 3251 3848 4635 4903 1239 Size: 393 815 804 597 787 268 1524 Fragments arranged by size: 1524 815 804 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2462 Size: 5188 EcoO109I rG'GnC_Cy Cuts at: 2266 2266 Size: 5188 EcoRI G'AATT_C Cuts at: 2497 2875 2497 Size: 378 4810 FspI TGC'GCA Cuts at: 3694 3694 Size: 5188 HaeII r_GCGC'y Cuts at: 1678 4335 1678 Size: 2657 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2092 2124 2465 2497 3829 Size: 32 135 32 520 32 341 32 1332 Cuts at: 3829 3861 3903 3935 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1332 520 341 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2532 2778 669 Size: 988 281 594 246 3079 Fragments arranged by size: 3079 988 594 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5188 HpaI GTT'AAC Cuts at: 2778 2778 Size: 5188 KpnI G_GTAC'C Cuts at: 2513 2513 Size: 5188 MfeI C'AATT_G Cuts at: 2787 2787 Size: 5188 MluI A'CGCG_T Cuts at: 5064 5064 Size: 5188 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4302 4486 1305 Size: 2997 184 2007 Fragments arranged by size: 2997 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2015 2334 10 Size: 54 1425 343 183 319 2864 Fragments arranged by size: 2864 1425 343 319 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 3146 3505 3664 518 Size: 648 1980 359 159 2042 Fragments arranged by size: 2042 1980 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2604 3281 4429 4674 1606 Size: 998 677 1148 245 2120 Fragments arranged by size: 2120 1148 998 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4636 NdeI CA'TA_TG Cuts at: 387 387 Size: 5188 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5188 NotI GC'GGCC_GC Cuts at: 2635 2635 Size: 5188 NspI r_CATG'y Cuts at: 1132 1229 2546 4091 1132 Size: 97 1317 1545 2229 Fragments arranged by size: 2229 1545 1317 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5188 PciI A'CATG_T Cuts at: 4087 4087 Size: 5188 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5188 PmeI GTTT'AAAC Cuts at: 2492 2492 Size: 5188 PmlI CAC'GTG Cuts at: 2188 2313 2188 Size: 125 5063 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3257 3289 4770 4802 3257 Size: 32 1481 32 3643 Fragments arranged by size: 3643 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2266 2266 Size: 5188 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5188 PsiI TTA'TAA Cuts at: 2758 2758 Size: 5188 PstI C_TGCA'G Cuts at: 830 2540 830 Size: 1710 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5188 SalI G'TCGA_C Cuts at: 1936 2530 1936 Size: 594 4594 SbfI CC_TGCA'GG Cuts at: 2540 2540 Size: 5188 ScaI AGT'ACT Cuts at: 3436 5148 3436 Size: 1712 3476 SfcI C'TryA_G Cuts at: 826 1045 2536 3671 4352 4543 826 Size: 219 1491 1135 681 191 1471 Fragments arranged by size: 1491 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5188 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5188 SmaI CCC'GGG Cuts at: 1872 2515 1872 Size: 643 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3292 4193 4455 820 Size: 197 926 1349 901 262 1553 Fragments arranged by size: 1553 1349 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2979 493 Size: 2486 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5188 SphI G_CATG'C Cuts at: 1132 2546 1132 Size: 1414 3774 SspI AAT'ATT Cuts at: 5 52 3112 5 Size: 47 3060 2081 Fragments arranged by size: 3060 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5188 StyI C'CwwG_G Cuts at: 513 1065 2627 513 Size: 552 1562 3074 Fragments arranged by size: 3074 1562 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 2359 3215 3232 3385 3570 4015 Size: 1255 578 201 856 17 153 185 445 Cuts at: 4015 325 Size: 1498 Fragments arranged by size: 1498 1255 856 578 445 201 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3434 5146 96 Size: 274 80 33 51 1443 1457 1712 138 Fragments arranged by size: 1712 1457 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5148 5180 5148 Size: 32 5156 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5188 XbaI T'CTAG_A Cuts at: 2524 2524 Size: 5188 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5188 XmaI C'CCGG_G Cuts at: 1870 2513 1870 Size: 643 4545 XmnI GAAnn'nnTTC Cuts at: 3317 3317 Size: 5188 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2996 276 Size: 53 83 186 936 1462 2468 Fragments arranged by size: 2468 1462 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI