Restriction map for
pFN21AE0101
##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BlnI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoT22I FauI FbaI Fnu4HI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
ItaI KasI KpnI Ksp632I KspI
MaeI MaeII MboII MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TseI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AgeI 38 ACCGGT A^CCGGT
AlwI 252 GATCC GGATC(4/5)
AlwNI 395 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 388 ATTAAT AT^TAAT
AsnI 388 ATTAAT AT^TAAT
AvaI 336 CTCGGG C^[CT]CG[GA]G
BalI 287 TGGCCA TGG^CCA
BanI 78 GGTGCC G^G[CT][GA]CC
BglI 403 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 21 AGATCT A^GATCT
BlpI 293 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 293 GCTTAGC GC^T[ACGT]AGC
BsaWI 38 ACCGGT [AT]^CCGG[AT]
BseRI 179 GAGGAG GAGGAG(10/8)
BsmAI 182 GAGAC GTCTC(1/5)
BsmFI 339 GGGAC GGGAC(10/14)
BsoBI 336 CTCGGG C^[CT]CG[GA]G
BsrFI 38 ACCGGT [GA]^CCGG[CT]
BsrI 406 ACTGG ACTGG(1/-1)
BstAPI 412 GCATATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 480 GGTCACC G^GT[ACGT]ACC
BstF5I 30 CATCC GGATG(2/0)
BstPI 480 GGTCACC G^GT[ACGT]ACC
BstYI 21 AGATCT [GA]^GATC[CT]
CelII 293 GCTTAGC GC^T[ACGT]AGC
Cfr10I 38 ACCGGT [GA]^CCGG[CT]
EaeI 287 TGGCCA [CT]^GGCC[GA]
EcoO65I 480 GGTCACC G^GT[ACGT]ACC
EcoRV 460 GATATC GAT^ATC
FokI 30 CATCC GGATG(9/13)
HaeIII 288 GGCC GG^CC
HapII 39 CCGG C^CGG
HpaII 39 CCGG C^CGG
MflI 21 AGATCT [GA]^GATC[CT]
MluNI 287 TGGCCA TGG^CCA
MscI 287 TGGCCA TGG^CCA
MseI 389 TTAA T^TAA
MspI 39 CCGG C^CGG
NlaIV 78 GGTGCC GG[ACGT]^[ACGT]CC
PinAI 38 ACCGGT A^CCGGT
PleI 150 GACTC GAGTC(4/5)
PshBI 388 ATTAAT AT^TAAT
SfcI 473 CTACAG C^T[GA][CT]AG
SgrAI 37 CACCGGTG C[GA]^CCGG[CT]G
SmlI 145 CTCAAG C^T[CT][GA]AG
Tru9I 389 TTAA T^TAA
XhoII 21 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BsaBI 190 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
456 GATGGATATC
BsgI 25 CTGCAC GTGCAG(16/14)
105 CTGCAC
Cac8I 54 GCCAGC GC[ACGT]^[ACGT]GC
289 GCCAGC
DdeI 294 CTTAG C^T[ACGT]AG
356 CTGAG
DpnII 22 GATC ^GATC
252 GATC
DsaI 255 CCATGG C^C[GA][CT]GG
274 CCATGG
HphI 160 GGTGA GGTGA(8/7)
482 TCACC
MaeIII 223 GTGAC ^GT[ACGT]AC
481 GTCAC
MamI 190 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
456 GATGGATATC
MboI 22 GATC ^GATC
252 GATC
MslI 35 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
483 CACCTATGTG
NcoI 255 CCATGG C^CATGG
274 CCATGG
NdeII 22 GATC ^GATC
252 GATC
Sau3AI 22 GATC ^GATC
252 GATC
TaqI 188 TCGA T^CGA
530 TCGA
TfiI 195 GAATC G^A[AT]TC
527 GAATC
Tsp45I 223 GTGAC ^GT[GC]AC
481 GTCAC
TspRI 405 CACTG CA[GC]TG[ACGT][ACGT]^
476 CAGTG
TthHB8I 188 TCGA T^CGA
530 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BssKI
BstNI EcoRII EcoT14I HinfI MnlI
MvaI MwoI NlaIII ScrFI StyI
Tsp509I