Restriction map for
pFN21AB3282
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
AscI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I FauI FseI FspI HapII
HgaI Hin1I HpaI HpaII KasI
KspI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 494 GGTACC G^GTACC
AccI 538 GTAGAC GT^[AC][GT]AC
AclI 1007 AACGTT AA^CGTT
Alw44I 883 GTGCAC G^TGCAC
ApaI 763 GGGCCC GGGCC^C
ApaLI 883 GTGCAC G^TGCAC
AseI 438 ATTAAT AT^TAAT
AsnI 438 ATTAAT AT^TAAT
Asp700I 1441 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 494 GGTACC G^GTACC
AspHI 883 GTGCAC G[AT]GC[AT]^C
BanI 494 GGTACC G^G[CT][GA]CC
BcgI 1919 GCATTGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 587 TGATCA T^GATCA
BglII 1734 AGATCT A^GATCT
BsaI 208 GAGACC GGTCTC(1/5)
BsgI 325 CTGCAC GTGCAG(16/14)
BsiHKAI 883 GTGCAC G[AT]GC[AT]^C
BsmFI 1532 GTCCC GGGAC(10/14)
Bsp1407I 1268 TGTACA T^GTACA
BspHI 1022 TCATGA T^CATGA
BsrDI 199 CATTGC GCAATG(2/0)
BsrGI 1268 TGTACA T^GTACA
BstYI 1734 AGATCT [GA]^GATC[CT]
CfoI 2049 GCGC GCG^C
DraI 129 TTTAAA TTT^AAA
DraIII 328 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 144 CCACGG C^C[GA][CT]GG
EarI 1558 CTCTTC CTCTTC(1/4)
FbaI 587 TGATCA T^GATCA
HaeII 2048 GGCGCT [GA]GCGC^[CT]
HhaI 2049 GCGC GCG^C
HinP1I 2049 GCGC G^CGC
HincII 478 GTCAAC GT[CT]^[GA]AC
HindII 478 GTCAAC GT[CT]^[GA]AC
HindIII 971 AAGCTT A^AGCTT
KpnI 494 GGTACC GGTAC^C
Ksp632I 1558 CTCTTC CTCTTC(1/4)
MflI 1734 AGATCT [GA]^GATC[CT]
MslI 544 CACGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 23 CAGCTG C[AC]G^C[GT]G
NheI 2118 GCTAGC G^CTAGC
PshBI 438 ATTAAT AT^TAAT
Psp1406I 1007 AACGTT AA^CGTT
PstI 176 CTGCAG CTGCA^G
PvuII 23 CAGCTG CAG^CTG
RcaI 1022 TCATGA T^CATGA
SfcI 176 CTGCAG C^T[GA][CT]AG
SspBI 1268 TGTACA T^GTACA
XcmI 1347 CCATAGCAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1734 AGATCT [GA]^GATC[CT]
XmnI 1441 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 85 GCGG CCGC(-3/-1)
876 GCGG
AlwI 1422 GGATC GGATC(4/5)
1674 GGATC
AlwNI 20 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1033 CAGTTCCTG
AvaI 888 CTCGAG C^[CT]CG[GA]G
1262 CTCGAG
AvaII 41 GGACC G^G[AT]CC
1531 GGTCC
BalI 141 TGGCCA TGG^CCA
1125 TGGCCA
BanII 763 GGGCCC G[GA]GC[CT]^C
1576 GGGCTC
BciVI 2064 GGATAC GTATCC(6/5)
1745 GTATCC
BpmI 233 CTGGAG CTGGAG(16/14)
1193 CTGGAG
BsaBI 456 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1417 GATGTGGATC
BsaJI 144 CCACGG C^C[ACGT][ACGT]GG
418 CCTCGG
BseRI 1616 CTCCTC GAGGAG(10/8)
746 GAGGAG
BsoBI 888 CTCGAG C^[CT]CG[GA]G
1262 CTCGAG
EaeI 141 TGGCCA [CT]^GGCC[GA]
1125 TGGCCA
EcoRI 1565 GAATTC G^AATTC
2126 GAATTC
EcoT22I 1513 ATGCAT ATGCA^T
1708 ATGCAT
HphI 1117 GGTGA GGTGA(8/7)
2035 GGTGA
MaeII 1008 ACGT A^CGT
1743 ACGT
MamI 456 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1417 GATGTGGATC
MfeI 758 CAATTG C^AATTG
2059 CAATTG
MluNI 141 TGGCCA TGG^CCA
1125 TGGCCA
MscI 141 TGGCCA TGG^CCA
1125 TGGCCA
MunI 758 CAATTG C^AATTG
2059 CAATTG
NsiI 1513 ATGCAT ATGCA^T
1708 ATGCAT
PaeR7I 888 CTCGAG C^TCGAG
1262 CTCGAG
PflMI 654 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1731 CCAAGATCTGG
Van91I 654 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1731 CCAAGATCTGG
XhoI 888 CTCGAG C^TCGAG
1262 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BbvI BfaI BmyI BpuAI BsiYI
BslI BsmAI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I