(Circular) (Six-base) MAPSORT of: pFN21AB3282.seq Check: 8686 from: 1 to: 6925 pFN21AB3282 6925 bp With 182 enzymes: SgfI * February 27, 2009 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4735 278 Size: 53 83 186 936 3199 2468 Fragments arranged by size: 3199 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2544 4268 1937 Size: 607 1724 4594 Fragments arranged by size: 4594 1724 607 Acc65I G'GTAC_C Cuts at: 2499 4246 2499 Size: 1747 5178 AclI AA'CG_TT Cuts at: 1563 3013 5052 5425 1563 Size: 1450 2039 373 3063 Fragments arranged by size: 3063 2039 1450 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2512 2945 3550 4988 6372 6640 1632 Size: 880 433 605 1438 1384 268 1917 Fragments arranged by size: 1917 1438 1384 880 605 433 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6728 AflIII A'CryG_T Cuts at: 1203 5824 6801 1203 Size: 4621 977 1327 Fragments arranged by size: 4621 1327 977 AgeI A'CCGG_T Cuts at: 5756 5756 Size: 6925 AhdI GACnn_n'nnGTC Cuts at: 5654 5654 Size: 6925 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6925 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6925 AlwNI CAG_nnn'CTG Cuts at: 2030 3043 4668 6240 2030 Size: 1013 1625 1572 2715 Fragments arranged by size: 2715 1625 1572 1013 ApaI G_GGCC'C Cuts at: 2772 2772 Size: 6925 ApaLI G'TGCA_C Cuts at: 2888 4982 6138 2888 Size: 2094 1156 3675 Fragments arranged by size: 3675 2094 1156 ApoI r'AATT_y Cuts at: 1493 2283 2834 3184 3570 3969 4131 4170 4234 Size: 790 551 350 386 399 162 39 64 Cuts at: 4234 4460 4612 6721 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 790 551 399 386 350 226 162 152 64 39 AseI AT'TA_AT Cuts at: 160 1032 2444 5479 160 Size: 872 1412 3035 1606 Fragments arranged by size: 3035 1606 1412 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 AvaI C'yCGr_G Cuts at: 1870 1943 2893 3267 4250 1870 Size: 73 950 374 983 4545 Fragments arranged by size: 4545 983 950 374 73 BamHI G'GATC_C Cuts at: 4255 4622 4255 Size: 367 6558 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2499 4246 5701 618 Size: 325 217 627 712 1747 1455 1842 Fragments arranged by size: 1842 1747 1455 712 627 325 217 BanII G_rGCy'C Cuts at: 1391 2772 3585 4244 1391 Size: 1381 813 659 4072 Fragments arranged by size: 4072 1381 813 659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3219 3665 4188 928 Size: 971 1320 446 523 3665 Fragments arranged by size: 3665 1320 971 523 446 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 5116 5150 1903 Size: 34 1976 34 1169 34 3678 Fragments arranged by size: 3678 1976 1169 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 4063 4817 6027 3761 Size: 302 754 1210 4659 Fragments arranged by size: 4659 1210 754 302 BclI T'GATC_A Cuts at: 1548 2592 1548 Size: 1044 5881 BfrBI ATG'CAT Cuts at: 3520 3715 3520 Size: 195 6730 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5536 136 Size: 107 122 71 631 605 3864 1525 Fragments arranged by size: 3864 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3739 6920 3739 Size: 3181 3744 BlpI GC'TnA_GC Cuts at: 4342 4342 Size: 6925 Bme1580I G_kGCm'C Cuts at: 1790 2772 2892 4986 6142 1790 Size: 982 120 2094 1156 2573 Fragments arranged by size: 2573 2094 1156 982 120 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6925 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4656 5614 442 Size: 416 53 83 399 24 3239 958 1753 Fragments arranged by size: 3239 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4127 1056 Size: 3071 3854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2259 3219 5585 1239 Size: 1020 960 2366 2579 Fragments arranged by size: 2579 2366 1020 960 Bpu10I CC'TnA_GC Cuts at: 3369 3369 Size: 6925 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2335 5050 5915 6213 2335 Size: 2715 865 298 3047 Fragments arranged by size: 3047 2715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2207 5588 882 Size: 617 708 3381 2219 Fragments arranged by size: 3381 2219 708 617 BsaAI yAC'GTr Cuts at: 493 1204 4716 493 Size: 711 3512 2702 Fragments arranged by size: 3512 2702 711 BsaBI GATnn'nnATC Cuts at: 2465 3426 6919 2465 Size: 961 3493 2471 Fragments arranged by size: 3493 2471 961 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4732 5114 5749 275 Size: 53 83 186 936 3199 382 635 1451 Fragments arranged by size: 3199 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5358 5756 6030 6177 6588 5358 Size: 398 274 147 411 5695 Fragments arranged by size: 5695 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3052 3082 3357 3387 3465 Size: 30 165 30 1649 30 275 30 78 Cuts at: 3465 3495 1178 Size: 30 4608 Fragments arranged by size: 4608 1649 275 165 78 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2766 3612 1186 Size: 1580 846 4499 Fragments arranged by size: 4499 1580 846 BseYI C'CCAG_C Cuts at: 6128 6624 6128 Size: 496 6429 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 1163 Size: 573 579 5773 Fragments arranged by size: 5773 579 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4375 5136 6164 1540 Size: 275 186 2374 761 1028 2301 Fragments arranged by size: 2374 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2892 4986 5071 6142 2892 Size: 2094 85 1071 3675 Fragments arranged by size: 3675 2094 1071 85 BsmI GAATG_Cn' Cuts at: 2251 2513 3522 4436 4529 2251 Size: 262 1009 914 93 4647 Fragments arranged by size: 4647 1009 914 262 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2772 2892 3585 4244 4986 5071 6142 Size: 399 982 120 693 659 742 85 1071 Cuts at: 6142 1391 Size: 2174 Fragments arranged by size: 2174 1071 982 742 693 659 399 120 85 BspEI T'CCGG_A Cuts at: 6588 6588 Size: 6925 BspHI T'CATG_A Cuts at: 3027 4814 6544 3027 Size: 1787 1730 3408 Fragments arranged by size: 3408 1787 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4280 844 Size: 3436 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4812 1394 Size: 429 2989 3507 Fragments arranged by size: 3507 2989 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2203 5420 5594 65 Size: 1183 955 3217 174 1396 Fragments arranged by size: 3217 1396 1183 955 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5569 5756 1700 Size: 110 3759 187 2869 Fragments arranged by size: 3759 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3273 96 Size: 3177 3748 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6925 BssSI C'ACGA_G Cuts at: 4985 5997 4985 Size: 1012 5913 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6925 BstBI TT'CG_AA Cuts at: 5729 5729 Size: 6925 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6925 BstYI r'GATC_y Cuts at: 1972 3739 4255 4622 5007 5024 6476 6562 6574 Size: 1767 516 367 385 17 1452 86 12 Cuts at: 6574 6920 1972 Size: 346 1977 Fragments arranged by size: 1977 1767 1452 516 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2149 513 Size: 552 1084 5289 Fragments arranged by size: 5289 1084 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6186 Fragments arranged by size: 6186 683 56 BtsI GCAGTG_nn' Cuts at: 2802 3073 3099 3174 3261 4441 5235 5255 2802 Size: 271 26 75 87 1180 794 20 4472 Fragments arranged by size: 4472 1180 794 271 87 75 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6925 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3529 3564 529 Size: 35 2965 35 3890 Fragments arranged by size: 3890 2965 35 35 DraI TTT'AAA Cuts at: 2136 4229 2136 Size: 2093 4832 DraIII CAC_nnn'GTG Cuts at: 2338 2338 Size: 6925 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5932 809 Size: 507 4616 1802 Fragments arranged by size: 4616 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2146 3130 4372 Size: 54 1425 50 275 18 316 984 1242 Cuts at: 4372 5261 6890 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1242 984 889 316 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4372 1537 Size: 275 2560 4090 Fragments arranged by size: 4090 2560 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3569 4855 1353 Size: 2216 1286 3423 Fragments arranged by size: 3423 2216 1286 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5512 5886 6032 387 Size: 1064 138 3923 374 146 1280 Fragments arranged by size: 3923 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2259 2512 2945 3219 3550 4988 5585 Size: 393 627 253 433 274 331 1438 597 Cuts at: 5585 6372 6640 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1438 787 627 597 433 393 331 274 268 253 EcoRI G'AATT_C Cuts at: 3570 4131 4234 4612 3570 Size: 561 103 378 5883 Fragments arranged by size: 5883 561 378 103 FspI TGC'GCA Cuts at: 5431 5431 Size: 6925 HaeII r_GCGC'y Cuts at: 1678 4057 6072 1678 Size: 2379 2015 2531 Fragments arranged by size: 2531 2379 2015 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3520 3552 3815 3847 4205 Size: 32 135 32 1948 32 263 32 358 Cuts at: 4205 4237 5566 5598 5640 5672 1373 Size: 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1948 1329 358 263 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2485 4269 4515 669 Size: 988 281 547 1784 246 3079 Fragments arranged by size: 3079 1784 988 547 281 246 HindIII A'AGCT_T Cuts at: 748 2976 748 Size: 2228 4697 HpaI GTT'AAC Cuts at: 4515 4515 Size: 6925 KpnI G_GTAC'C Cuts at: 2503 4250 2503 Size: 1747 5178 MfeI C'AATT_G Cuts at: 2763 4064 4524 2763 Size: 1301 460 5164 Fragments arranged by size: 5164 1301 460 MluI A'CGCG_T Cuts at: 6801 6801 Size: 6925 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6039 6223 1305 Size: 4734 184 2007 Fragments arranged by size: 4734 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2148 3132 10 Size: 54 1425 343 316 984 3803 Fragments arranged by size: 3803 1425 984 343 316 54 MslI CAynn'nnrTG Cuts at: 518 1166 2553 4883 5242 5401 518 Size: 648 1387 2330 359 159 2042 Fragments arranged by size: 2330 2042 1387 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 4341 5018 6166 6411 1606 Size: 424 2311 677 1148 245 2120 Fragments arranged by size: 2311 2120 1148 677 424 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6373 NdeI CA'TA_TG Cuts at: 387 2013 2095 3630 387 Size: 1626 82 1535 3682 Fragments arranged by size: 3682 1626 1535 82 NheI G'CTAG_C Cuts at: 1052 4123 1052 Size: 3071 3854 NotI GC'GGCC_GC Cuts at: 4372 4372 Size: 6925 NsiI A_TGCA'T Cuts at: 3522 3717 3522 Size: 195 6730 NspI r_CATG'y Cuts at: 1132 1229 4283 5828 1132 Size: 97 3054 1545 2229 Fragments arranged by size: 3054 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6925 PciI A'CATG_T Cuts at: 5824 5824 Size: 6925 PflMI CCAn_nnn'nTGG Cuts at: 1108 2665 3742 1108 Size: 1557 1077 4291 Fragments arranged by size: 4291 1557 1077 PmeI GTTT'AAAC Cuts at: 4229 4229 Size: 6925 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4994 5026 6507 6539 4994 Size: 32 1481 32 5380 Fragments arranged by size: 5380 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6925 PsiI TTA'TAA Cuts at: 2586 3318 4495 2586 Size: 732 1177 5016 Fragments arranged by size: 5016 1177 732 PspOMI G'GGCC_C Cuts at: 2768 2768 Size: 6925 PspXI vC'TCGA_Gb Cuts at: 3267 3267 Size: 6925 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3738 3770 3738 Size: 32 6893 PstI C_TGCA'G Cuts at: 830 2185 4277 830 Size: 1355 2092 3478 Fragments arranged by size: 3478 2092 1355 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6925 PvuII CAG'CTG Cuts at: 2030 2030 Size: 6925 SalI G'TCGA_C Cuts at: 1936 4267 1936 Size: 2331 4594 SbfI CC_TGCA'GG Cuts at: 4277 4277 Size: 6925 ScaI AGT'ACT Cuts at: 5173 6885 5173 Size: 1712 5213 SfcI C'TryA_G Cuts at: 826 1045 2181 4273 5408 6089 6280 826 Size: 219 1136 2092 1135 681 191 1471 Fragments arranged by size: 2092 1471 1136 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6925 SmaI CCC'GGG Cuts at: 1872 4252 1872 Size: 2380 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2350 2893 3267 5029 5930 6192 Size: 197 926 407 543 374 1762 901 262 Cuts at: 6192 820 Size: 1553 Fragments arranged by size: 1762 1553 926 901 543 407 374 262 197 SnaBI TAC'GTA Cuts at: 493 4716 493 Size: 4223 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6925 SphI G_CATG'C Cuts at: 1132 4283 1132 Size: 3151 3774 SspI AAT'ATT Cuts at: 5 52 4849 5 Size: 47 4797 2081 Fragments arranged by size: 4797 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6925 StyI C'CwwG_G Cuts at: 513 1065 4364 513 Size: 552 3299 3074 Fragments arranged by size: 3299 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4952 4969 5122 5307 5752 325 Size: 1255 3372 17 153 185 445 1498 Fragments arranged by size: 3372 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3273 5171 6883 Size: 274 80 33 51 1443 1296 1898 1712 Cuts at: 6883 96 Size: 138 Fragments arranged by size: 1898 1712 1443 1296 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2382 2414 2663 2695 2778 2810 3052 3084 3465 Size: 32 249 32 83 32 242 32 381 Cuts at: 3465 3497 6885 6917 2382 Size: 32 3388 32 2390 Fragments arranged by size: 3388 2390 381 249 242 83 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6925 XbaI T'CTAG_A Cuts at: 4261 4261 Size: 6925 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3359 3359 Size: 6925 XhoI C'TCGA_G Cuts at: 1943 2893 3267 1943 Size: 950 374 5601 Fragments arranged by size: 5601 950 374 XmaI C'CCGG_G Cuts at: 1870 4250 1870 Size: 2380 4545 XmnI GAAnn'nnTTC Cuts at: 3450 5054 3450 Size: 1604 5321 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4733 276 Size: 53 83 186 936 3199 2468 Fragments arranged by size: 3199 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI