Restriction map for
pFN21AB3282
(Linear) (Six-base) MAPSORT of: KIBB3282.seq Check: 3202 from: 1 to: 2220
KIBB3282.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 0 539 2220
Size: 539 1681
Acc65I G'GTAC_C
Cuts at: 0 494 2220
Size: 494 1726
AclI AA'CG_TT
Cuts at: 0 1008 2220
Size: 1008 1212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 940 1545 2220
Size: 507 433 605 675
Fragments arranged by size:
675 605 507 433
AlwNI CAG_nnn'CTG
Cuts at: 0 25 1038 2220
Size: 25 1013 1182
Fragments arranged by size:
1182 1013 25
ApaI G_GGCC'C
Cuts at: 0 767 2220
Size: 767 1453
ApaLI G'TGCA_C
Cuts at: 0 883 2220
Size: 883 1337
ApoI r'AATT_y
Cuts at: 0 278 829 1179 1565 1964 2126 2165 2220
Size: 278 551 350 386 399 162 39 55
Fragments arranged by size:
551 399 386 350 278 162 55 39
AseI AT'TA_AT
Cuts at: 0 439 2220
Size: 439 1781
AvaI C'yCGr_G
Cuts at: 0 888 1262 2220
Size: 888 374 958
Fragments arranged by size:
958 888 374
BanI G'GyrC_C
Cuts at: 0 494 2220
Size: 494 1726
BanII G_rGCy'C
Cuts at: 0 767 1580 2220
Size: 767 813 640
Fragments arranged by size:
813 767 640
BbsI GAAGACnn'nnnn_
Cuts at: 0 1214 1660 2183 2220
Size: 1214 446 523 37
Fragments arranged by size:
1214 523 446 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1908 1942 2220
Size: 1908 34 278
Fragments arranged by size:
1908 278 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1756 2058 2220
Size: 1756 302 162
Fragments arranged by size:
1756 302 162
BclI T'GATC_A
Cuts at: 0 587 2220
Size: 587 1633
BfrBI ATG'CAT
Cuts at: 0 1515 1710 2220
Size: 1515 195 510
Fragments arranged by size:
1515 510 195
BglII A'GATC_T
Cuts at: 0 1734 2220
Size: 1734 486
Bme1580I G_kGCm'C
Cuts at: 0 767 887 2220
Size: 767 120 1333
Fragments arranged by size:
1333 767 120
BmtI G_CTAG'C
Cuts at: 0 2122 2220
Size: 2122 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1214 2220
Size: 254 960 1006
Fragments arranged by size:
1006 960 254
Bpu10I CC'TnA_GC
Cuts at: 0 1364 2220
Size: 1364 856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 2220
Size: 330 1890
BsaI GGTCTCn'nnnn_
Cuts at: 0 202 2220
Size: 202 2018
BsaBI GATnn'nnATC
Cuts at: 0 460 1421 2220
Size: 460 961 799
Fragments arranged by size:
961 799 460
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1047 1077 1352 1382 1460 1490 2220
Size: 1047 30 275 30 78 30 730
Fragments arranged by size:
1047 730 275 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 761 1607 2220
Size: 761 846 613
Fragments arranged by size:
846 761 613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 2220
Size: 310 1910
BsiHKAI G_wGCw'C
Cuts at: 0 887 2220
Size: 887 1333
BsmI GAATG_Cn'
Cuts at: 0 246 508 1517 2220
Size: 246 262 1009 703
Fragments arranged by size:
1009 703 262 246
Bsp1286I G_dGCh'C
Cuts at: 0 767 887 1580 2220
Size: 767 120 693 640
Fragments arranged by size:
767 693 640 120
BspHI T'CATG_A
Cuts at: 0 1022 2220
Size: 1022 1198
BsrDI GCAATG_nn'
Cuts at: 0 198 2220
Size: 198 2022
BsrGI T'GTAC_A
Cuts at: 0 1268 2220
Size: 1268 952
BstYI r'GATC_y
Cuts at: 0 1734 2220
Size: 1734 486
BtgI C'CryG_G
Cuts at: 0 144 2220
Size: 144 2076
BtsI GCAGTG_nn'
Cuts at: 0 797 1068 1094 1169 1256 2220
Size: 797 271 26 75 87 964
Fragments arranged by size:
964 797 271 87 75 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1524 1559 2220
Size: 1524 35 661
Fragments arranged by size:
1524 661 35
DraI TTT'AAA
Cuts at: 0 131 2220
Size: 131 2089
DraIII CAC_nnn'GTG
Cuts at: 0 333 2220
Size: 333 1887
EaeI y'GGCC_r
Cuts at: 0 141 1125 2220
Size: 141 984 1095
Fragments arranged by size:
1095 984 141
EarI CTCTTCn'nnn_
Cuts at: 0 1564 2220
Size: 1564 656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 507 940 1214 1545 2220
Size: 254 253 433 274 331 675
Fragments arranged by size:
675 433 331 274 254 253
EcoRI G'AATT_C
Cuts at: 0 1565 2126 2220
Size: 1565 561 94
Fragments arranged by size:
1565 561 94
HaeII r_GCGC'y
Cuts at: 0 2052 2220
Size: 2052 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1515 1547 1810 1842 2200 2220
Size: 1515 32 263 32 358 20
Fragments arranged by size:
1515 358 263 32 32 20
HincII GTy'rAC
Cuts at: 0 480 2220
Size: 480 1740
HindIII A'AGCT_T
Cuts at: 0 971 2220
Size: 971 1249
KpnI G_GTAC'C
Cuts at: 0 498 2220
Size: 498 1722
MfeI C'AATT_G
Cuts at: 0 758 2059 2220
Size: 758 1301 161
Fragments arranged by size:
1301 758 161
MscI TGG'CCA
Cuts at: 0 143 1127 2220
Size: 143 984 1093
Fragments arranged by size:
1093 984 143
MslI CAynn'nnrTG
Cuts at: 0 548 2220
Size: 548 1672
MspA1I CmG'CkG
Cuts at: 0 25 2220
Size: 25 2195
NdeI CA'TA_TG
Cuts at: 0 8 90 1625 2220
Size: 8 82 1535 595
Fragments arranged by size:
1535 595 82 8
NheI G'CTAG_C
Cuts at: 0 2118 2220
Size: 2118 102
NsiI A_TGCA'T
Cuts at: 0 1517 1712 2220
Size: 1517 195 508
Fragments arranged by size:
1517 508 195
PflMI CCAn_nnn'nTGG
Cuts at: 0 660 1737 2220
Size: 660 1077 483
Fragments arranged by size:
1077 660 483
PsiI TTA'TAA
Cuts at: 0 581 1313 2220
Size: 581 732 907
Fragments arranged by size:
907 732 581
PspOMI G'GGCC_C
Cuts at: 0 763 2220
Size: 763 1457
PspXI vC'TCGA_Gb
Cuts at: 0 1262 2220
Size: 1262 958
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1733 1765 2220
Size: 1733 32 455
Fragments arranged by size:
1733 455 32
PstI C_TGCA'G
Cuts at: 0 180 2220
Size: 180 2040
PvuII CAG'CTG
Cuts at: 0 25 2220
Size: 25 2195
SfcI C'TryA_G
Cuts at: 0 176 2220
Size: 176 2044
SmlI C'TyrA_G
Cuts at: 0 345 888 1262 2220
Size: 345 543 374 958
Fragments arranged by size:
958 543 374 345
TatI w'GTAC_w
Cuts at: 0 1268 2220
Size: 1268 952
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 377 409 658 690 773 805 1047 1079
Size: 377 32 249 32 83 32 242 32
Cuts at: 1079 1460 1492 2220
Size: 381 32 728
Fragments arranged by size:
728 381 377 249 242 83 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1354 2220
Size: 1354 866
XhoI C'TCGA_G
Cuts at: 0 888 1262 2220
Size: 888 374 958
Fragments arranged by size:
958 888 374
XmnI GAAnn'nnTTC
Cuts at: 0 1445 2220
Size: 1445 775
Enzymes that do cut:
AccI Acc65I AclI AcuI AlwNI ApaI ApaLI ApoI
AseI AvaI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglII Bme1580I BmtI BpmI Bpu10I BpuEI BsaI
BsaBI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrDI BsrGI BstYI BtgI BtsI CspCI DraI DraIII
EaeI EarI Eco57MI EcoRI HaeII Hin4I HincII HindIII
KpnI MfeI MscI MslI MspA1I NdeI NheI NsiI
PflMI PsiI PspOMI PspXI PsrI PstI PvuII SfcI
SmlI TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI AscI AsiSI AvrII BaeI BamHI BbeI
BbvCI BglI BlpI BmgBI BmrI BplI BsaAI BsaHI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HpaI KasI
MluI MmeI NaeI NarI NcoI NgoMIV NotI NruI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII Tth111I XbaI XmaI ZraI