Restriction map for
pFN21AB5207
(Linear) (Six-base) MAPSORT of: KIBB5207.seq Check: 6259 from: 1 to: 1587
KIBB5207.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1477 1587
Size: 1477 110
AatII G_ACGT'C
Cuts at: 0 218 1587
Size: 218 1369
Acc65I G'GTAC_C
Cuts at: 0 1443 1587
Size: 1443 144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 861 1471 1587
Size: 50 811 610 116
Fragments arranged by size:
811 610 116 50
AfeI AGC'GCT
Cuts at: 0 797 1587
Size: 797 790
ApaLI G'TGCA_C
Cuts at: 0 386 1367 1587
Size: 386 981 220
Fragments arranged by size:
981 386 220
ApoI r'AATT_y
Cuts at: 0 398 542 1587
Size: 398 144 1045
Fragments arranged by size:
1045 398 144
AvaI C'yCGr_G
Cuts at: 0 839 1587
Size: 839 748
BamHI G'GATC_C
Cuts at: 0 1194 1224 1587
Size: 1194 30 363
Fragments arranged by size:
1194 363 30
BanI G'GyrC_C
Cuts at: 0 169 1443 1587
Size: 169 1274 144
Fragments arranged by size:
1274 169 144
BbeI G_GCGC'C
Cuts at: 0 173 1587
Size: 173 1414
BbsI GAAGACnn'nnnn_
Cuts at: 0 1352 1410 1587
Size: 1352 58 177
Fragments arranged by size:
1352 177 58
BbvCI CC'TCA_GC
Cuts at: 0 503 1412 1587
Size: 503 909 175
Fragments arranged by size:
909 503 175
BclI T'GATC_A
Cuts at: 0 644 1587
Size: 644 943
BglI GCCn_nnn'nGGC
Cuts at: 0 43 1587
Size: 43 1544
BlpI GC'TnA_GC
Cuts at: 0 86 1169 1587
Size: 86 1083 418
Fragments arranged by size:
1083 418 86
Bme1580I G_kGCm'C
Cuts at: 0 390 840 1371 1587
Size: 390 450 531 216
Fragments arranged by size:
531 450 390 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 863 1587
Size: 863 724
BmtI G_CTAG'C
Cuts at: 0 580 1587
Size: 580 1007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 644 1285 1587
Size: 494 150 641 302
Fragments arranged by size:
641 494 302 150
Bpu10I CC'TnA_GC
Cuts at: 0 503 1412 1587
Size: 503 909 175
Fragments arranged by size:
909 503 175
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 875 1450 1587
Size: 268 607 575 137
Fragments arranged by size:
607 575 268 137
BsaHI Gr'CG_yC
Cuts at: 0 170 215 1587
Size: 170 45 1372
Fragments arranged by size:
1372 170 45
BseYI C'CCAG_C
Cuts at: 0 81 299 1173 1587
Size: 81 218 874 414
Fragments arranged by size:
874 414 218 81
BsiHKAI G_wGCw'C
Cuts at: 0 319 390 834 1371 1587
Size: 319 71 444 537 216
Fragments arranged by size:
537 444 319 216 71
BsmI GAATG_Cn'
Cuts at: 0 1578 1587
Size: 1578 9
Bsp1286I G_dGCh'C
Cuts at: 0 319 390 834 840 1371 1587
Size: 319 71 444 6 531 216
Fragments arranged by size:
531 444 319 216 71 6
BspHI T'CATG_A
Cuts at: 0 431 1587
Size: 431 1156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1477 1587
Size: 1477 110
BsrBI CCG'CTC
Cuts at: 0 106 1587
Size: 106 1481
BsrDI GCAATG_nn'
Cuts at: 0 735 1163 1310 1587
Size: 735 428 147 277
Fragments arranged by size:
735 428 277 147
BsrGI T'GTAC_A
Cuts at: 0 1068 1587
Size: 1068 519
BssSI C'ACGA_G
Cuts at: 0 833 1587
Size: 833 754
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1014 1587
Size: 1014 573
BstYI r'GATC_y
Cuts at: 0 1054 1194 1224 1277 1587
Size: 1054 140 30 53 310
Fragments arranged by size:
1054 310 140 53 30
BtgI C'CryG_G
Cuts at: 0 678 1263 1587
Size: 678 585 324
Fragments arranged by size:
678 585 324
BtsI GCAGTG_nn'
Cuts at: 0 212 1248 1587
Size: 212 1036 339
Fragments arranged by size:
1036 339 212
DraIII CAC_nnn'GTG
Cuts at: 0 829 1587
Size: 829 758
EaeI y'GGCC_r
Cuts at: 0 23 1004 1587
Size: 23 981 583
Fragments arranged by size:
981 583 23
EarI CTCTTCn'nnn_
Cuts at: 0 1568 1587
Size: 1568 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 494 644 861 1285 1471 1587
Size: 50 444 150 217 424 186 116
Fragments arranged by size:
444 424 217 186 150 116 50
EcoO109I rG'GnC_Cy
Cuts at: 0 44 466 716 1531 1587
Size: 44 422 250 815 56
Fragments arranged by size:
815 422 250 56 44
EcoRV GAT'ATC
Cuts at: 0 1308 1587
Size: 1308 279
HaeII r_GCGC'y
Cuts at: 0 173 799 1587
Size: 173 626 788
Fragments arranged by size:
788 626 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 455 487 769 801 1195 1227 1279 1311
Size: 455 32 282 32 394 32 52 32
Cuts at: 1311 1567 1587
Size: 256 20
Fragments arranged by size:
455 394 282 256 52 32 32 32
32 20
HincII GTy'rAC
Cuts at: 0 1355 1587
Size: 1355 232
KasI G'GCGC_C
Cuts at: 0 169 1587
Size: 169 1418
KpnI G_GTAC'C
Cuts at: 0 1447 1587
Size: 1447 140
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1587
Size: 133 1454
MscI TGG'CCA
Cuts at: 0 1006 1587
Size: 1006 581
MslI CAynn'nnrTG
Cuts at: 0 308 523 1012 1136 1253 1587
Size: 308 215 489 124 117 334
Fragments arranged by size:
489 334 308 215 124 117
MspA1I CmG'CkG
Cuts at: 0 28 1189 1587
Size: 28 1161 398
Fragments arranged by size:
1161 398 28
NarI GG'CG_CC
Cuts at: 0 170 1587
Size: 170 1417
NcoI C'CATG_G
Cuts at: 0 678 1587
Size: 678 909
NdeI CA'TA_TG
Cuts at: 0 1430 1587
Size: 1430 157
NheI G'CTAG_C
Cuts at: 0 576 1587
Size: 576 1011
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 1587
Size: 306 1281
PfoI T'CCnGG_A
Cuts at: 0 1301 1587
Size: 1301 286
PpuMI rG'GwC_Cy
Cuts at: 0 466 716 1531 1587
Size: 466 250 815 56
Fragments arranged by size:
815 466 250 56
PshAI GACnn'nnGTC
Cuts at: 0 115 1587
Size: 115 1472
PstI C_TGCA'G
Cuts at: 0 1286 1587
Size: 1286 301
PvuII CAG'CTG
Cuts at: 0 1189 1587
Size: 1189 398
SanDI GG'GwC_CC
Cuts at: 0 1531 1587
Size: 1531 56
SapI GCTCTTCn'nnn_
Cuts at: 0 1568 1587
Size: 1568 19
SbfI CC_TGCA'GG
Cuts at: 0 1286 1587
Size: 1286 301
ScaI AGT'ACT
Cuts at: 0 374 1587
Size: 374 1213
SexAI A'CCwGG_T
Cuts at: 0 825 1587
Size: 825 762
SfcI C'TryA_G
Cuts at: 0 1282 1587
Size: 1282 305
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 43 1587
Size: 43 1544
SfoI GGC'GCC
Cuts at: 0 171 1587
Size: 171 1416
SmaI CCC'GGG
Cuts at: 0 841 1587
Size: 841 746
StuI AGG'CCT
Cuts at: 0 1516 1587
Size: 1516 71
StyI C'CwwG_G
Cuts at: 0 590 678 1587
Size: 590 88 909
Fragments arranged by size:
909 590 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 949 1587
Size: 298 651 638
Fragments arranged by size:
651 638 298
TatI w'GTAC_w
Cuts at: 0 372 639 900 1068 1149 1587
Size: 372 267 261 168 81 438
Fragments arranged by size:
438 372 267 261 168 81
Tth111I GACn'n_nGTC
Cuts at: 0 1359 1587
Size: 1359 228
XbaI T'CTAG_A
Cuts at: 0 1200 1587
Size: 1200 387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 1170 1323 1587
Size: 285 885 153 264
Fragments arranged by size:
885 285 264 153
XmaI C'CCGG_G
Cuts at: 0 839 1587
Size: 839 748
XmnI GAAnn'nnTTC
Cuts at: 0 975 1587
Size: 975 612
ZraI GAC'GTC
Cuts at: 0 216 1587
Size: 216 1371
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI ApaLI ApoI AvaI
BamHI BanI BbeI BbsI BbvCI BclI BglI BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaHI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI
BssSI BstXI BstYI BtgI BtsI DraIII EaeI EarI
Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
PflMI PfoI PpuMI PshAI PstI PvuII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SmaI StuI
StyI TaqII TatI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BanII BcgI BciVI BfrBI BglII BmgBI BplI BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmBI BspEI BsrFI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HindIII
HpaI MfeI MluI NaeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI XhoI