(Circular) (Six-base) MAPSORT of: pFN21AB4148.seq Check: 4184 from: 1 to: 6277 pFN21AB4148 6277 bp With 182 enzymes: SgfI * February 27, 2009 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2362 2704 2362 Size: 342 5935 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2973 3620 1937 Size: 1036 647 4594 Fragments arranged by size: 4594 1036 647 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2145 2189 2459 2760 3189 4340 5724 5992 Size: 513 44 270 301 429 1151 1384 268 Cuts at: 5992 1632 Size: 1917 Fragments arranged by size: 1917 1384 1151 513 429 301 270 268 44 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 5176 6153 1203 Size: 3973 977 1327 Fragments arranged by size: 3973 1327 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AlwNI CAG_nnn'CTG Cuts at: 3361 4020 5592 3361 Size: 659 1572 4046 Fragments arranged by size: 4046 1572 659 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 2831 3228 3586 3812 3964 6073 1493 Size: 1338 397 358 226 152 2109 1697 Fragments arranged by size: 2109 1697 1338 397 358 226 152 AseI AT'TA_AT Cuts at: 160 1032 2936 4831 160 Size: 872 1904 1895 1606 Fragments arranged by size: 1904 1895 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2066 3602 1870 Size: 73 82 41 1536 4545 Fragments arranged by size: 4545 1536 82 73 41 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2953 3598 5053 618 Size: 325 217 627 1166 645 1455 1842 Fragments arranged by size: 1842 1455 1166 645 627 325 217 BanII G_rGCy'C Cuts at: 1391 2872 3596 1391 Size: 1481 724 4072 Fragments arranged by size: 4072 1481 724 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3070 3183 3250 928 Size: 971 1171 113 67 3955 Fragments arranged by size: 3955 1171 971 113 67 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2142 4169 5379 2142 Size: 2027 1210 3040 Fragments arranged by size: 3040 2027 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4888 136 Size: 107 122 71 631 605 3216 1525 Fragments arranged by size: 3216 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 4338 5494 1790 Size: 2548 1156 2573 Fragments arranged by size: 2573 2548 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4008 4966 442 Size: 416 53 83 399 24 2591 958 1753 Fragments arranged by size: 2591 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3366 4937 1239 Size: 2127 1571 2579 Fragments arranged by size: 2579 2127 1571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2865 3284 4402 5267 5565 2865 Size: 419 1118 865 298 3577 Fragments arranged by size: 3577 1118 865 419 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4940 882 Size: 617 3441 2219 Fragments arranged by size: 3441 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3084 4068 493 Size: 711 1880 984 2702 Fragments arranged by size: 2702 1880 984 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4084 4466 5101 275 Size: 53 83 186 936 2551 382 635 1451 Fragments arranged by size: 2551 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4710 5108 5382 5529 5940 4710 Size: 398 274 147 411 5047 Fragments arranged by size: 5047 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3096 3126 1178 Size: 30 165 30 1693 30 4329 Fragments arranged by size: 4329 1693 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3290 1186 Size: 2104 4173 BseYI C'CCAG_C Cuts at: 5480 5976 5480 Size: 496 5781 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2016 2580 1163 Size: 573 280 564 4860 Fragments arranged by size: 4860 573 564 280 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2872 4338 4423 5494 2872 Size: 1466 85 1071 3655 Fragments arranged by size: 3655 1466 1071 85 BsmI GAATG_Cn' Cuts at: 2492 3483 3788 3881 2492 Size: 991 305 93 4888 Fragments arranged by size: 4888 991 305 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2872 3596 4338 4423 5494 1391 Size: 399 1082 724 742 85 1071 2174 Fragments arranged by size: 2174 1082 1071 742 724 399 85 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 2872 4166 5896 2872 Size: 1294 1730 3253 Fragments arranged by size: 3253 1730 1294 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2362 2704 3632 844 Size: 1518 342 928 3489 Fragments arranged by size: 3489 1518 928 342 BsrBI CCG'CTC Cuts at: 1394 1823 4164 1394 Size: 429 2341 3507 Fragments arranged by size: 3507 2341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4772 4946 65 Size: 1183 3524 174 1396 Fragments arranged by size: 3524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 4921 5108 1700 Size: 110 210 2901 187 2869 Fragments arranged by size: 2901 2869 210 187 110 BsrGI T'GTAC_A Cuts at: 96 2901 3499 96 Size: 2805 598 2874 Fragments arranged by size: 2874 2805 598 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 3361 794 Size: 2567 3710 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6277 BstXI CCAn_nnnn'nTGG Cuts at: 3188 3188 Size: 6277 BstYI r'GATC_y Cuts at: 1972 3607 3974 4359 4376 5828 5914 5926 6272 Size: 1635 367 385 17 1452 86 12 346 Cuts at: 6272 1972 Size: 1977 Fragments arranged by size: 1977 1635 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2305 3550 513 Size: 552 939 301 1245 3240 Fragments arranged by size: 3240 1245 939 552 301 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2096 487 Size: 683 56 870 4668 Fragments arranged by size: 4668 870 683 56 BtsI GCAGTG_nn' Cuts at: 2829 3793 4587 4607 2829 Size: 964 794 20 4499 Fragments arranged by size: 4499 964 794 20 ClaI AT'CG_AT Cuts at: 1554 2858 1554 Size: 1304 4973 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 2604 3581 2604 Size: 977 5300 DraIII CAC_nnn'GTG Cuts at: 2182 2292 2182 Size: 110 6167 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2081 5284 809 Size: 507 765 3203 1802 Fragments arranged by size: 3203 1802 765 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2308 3724 Size: 54 1425 50 275 18 177 301 1416 Cuts at: 3724 4613 6242 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1416 889 301 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3438 4207 1353 Size: 2085 769 3423 Fragments arranged by size: 3423 2085 769 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2870 2870 Size: 6277 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 2189 2459 2760 3189 3366 4340 Size: 393 513 44 270 301 429 177 974 Cuts at: 4340 4937 5724 5992 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 974 787 597 513 429 393 301 270 268 177 44 EcoO109I rG'GnC_Cy Cuts at: 3304 3304 Size: 6277 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 6277 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 5424 1678 Size: 3746 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4918 4950 4992 5024 1373 Size: 32 135 32 3346 32 42 32 2626 Fragments arranged by size: 3346 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3621 3867 669 Size: 988 281 1683 246 3079 Fragments arranged by size: 3079 1683 988 281 246 HindIII A'AGCT_T Cuts at: 748 2559 2571 748 Size: 1811 12 4454 Fragments arranged by size: 4454 1811 12 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3237 3876 3237 Size: 639 5638 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3082 5391 5575 1305 Size: 1777 2309 184 2007 Fragments arranged by size: 2309 2007 1777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2310 10 Size: 54 1425 343 478 3977 Fragments arranged by size: 3977 1425 478 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3320 4235 4594 4753 518 Size: 648 2154 915 359 159 2042 Fragments arranged by size: 2154 2042 915 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3197 3693 4370 5518 5763 1606 Size: 1591 496 677 1148 245 2120 Fragments arranged by size: 2120 1591 1148 677 496 245 NaeI GCC'GGC Cuts at: 2022 2022 Size: 6277 NcoI C'CATG_G Cuts at: 513 1065 2004 2305 513 Size: 552 939 301 4485 Fragments arranged by size: 4485 939 552 301 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NgoMIV G'CCGG_C Cuts at: 2020 2020 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 3539 3635 5180 1132 Size: 97 2310 96 1545 2229 Fragments arranged by size: 2310 2229 1545 97 96 PasI CC'CwG_GG Cuts at: 1348 2154 1348 Size: 806 5471 PciI A'CATG_T Cuts at: 5176 5176 Size: 6277 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6277 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PmlI CAC'GTG Cuts at: 3084 3084 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 2811 3847 2811 Size: 1036 5241 PstI C_TGCA'G Cuts at: 830 3202 3437 3629 830 Size: 2372 235 192 3478 Fragments arranged by size: 3478 2372 235 192 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 3197 3197 Size: 6277 SacI G_AGCT'C Cuts at: 2872 2872 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SfcI C'TryA_G Cuts at: 826 1045 2893 2919 3198 3433 3625 4760 5441 Size: 219 1848 26 279 235 192 1135 681 Cuts at: 5441 5632 826 Size: 191 1471 Fragments arranged by size: 1848 1471 1135 681 279 235 219 192 191 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 2027 2068 3604 1872 Size: 155 41 1536 4545 Fragments arranged by size: 4545 1536 155 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 2880 3299 4381 5282 5544 820 Size: 197 926 937 419 1082 901 262 1553 Fragments arranged by size: 1553 1082 937 926 901 419 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SrfI GCCC'GGGC Cuts at: 2027 2027 Size: 6277 SspI AAT'ATT Cuts at: 5 52 2507 3022 4201 5 Size: 47 2455 515 1179 2081 Fragments arranged by size: 2455 2081 1179 515 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6277 StyI C'CwwG_G Cuts at: 513 1065 2004 2305 3716 513 Size: 552 939 301 1411 3074 Fragments arranged by size: 3074 1411 939 552 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4304 4321 4474 4659 5104 325 Size: 1255 2724 17 153 185 445 1498 Fragments arranged by size: 2724 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2901 3499 4523 Size: 274 80 33 51 1443 924 598 1024 Cuts at: 4523 6235 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1024 924 598 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3096 3128 6237 6269 3096 Size: 32 3109 32 3104 Fragments arranged by size: 3109 3104 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2078 1316 Size: 762 5515 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3051 3051 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 2025 2066 3602 1870 Size: 155 41 1536 4545 Fragments arranged by size: 4545 1536 155 41 XmnI GAAnn'nnTTC Cuts at: 4406 4406 Size: 6277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI