Restriction map for pFN21AB4148
 (Circular) (Six-base) MAPSORT of: pFN21AB4148.seq  Check: 4184  from: 1  to: 6277



pFN21AB4148  6277 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2362    2704    2362 
   Size:        342    5935
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4087     278 
   Size:         53      83     186     936    2551    2468

  Fragments arranged by size: 

                2551    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2973    3620    1937 
   Size:       1036     647    4594

  Fragments arranged by size: 

                4594    1036     647
 
Acc65I G'GTAC_C

Cuts at:   3598    3598 
   Size:       6277
 
AclI AA'CG_TT

Cuts at:   1563    4404    4777    1563 
   Size:       2841     373    3063

  Fragments arranged by size: 

                3063    2841     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2145    2189    2459    2760    3189    4340    5724    5992 
   Size:        513      44     270     301     429    1151    1384     268

Cuts at:   5992    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1384    1151     513     429     301     270     268
                  44
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6080
 
AflIII A'CryG_T

Cuts at:   1203    5176    6153    1203 
   Size:       3973     977    1327

  Fragments arranged by size: 

                3973    1327     977
 
AgeI A'CCGG_T

Cuts at:   5108    5108 
   Size:       6277
 
AhdI GACnn_n'nnGTC

Cuts at:   5006    5006 
   Size:       6277
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6277
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6277
 
AlwNI CAG_nnn'CTG

Cuts at:   3361    4020    5592    3361 
   Size:        659    1572    4046

  Fragments arranged by size: 

                4046    1572     659
 
ApaLI G'TGCA_C

Cuts at:   4334    5490    4334 
   Size:       1156    5121
 
ApoI r'AATT_y

Cuts at:   1493    2831    3228    3586    3812    3964    6073    1493 
   Size:       1338     397     358     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1338     397     358     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2936    4831     160 
   Size:        872    1904    1895    1606

  Fragments arranged by size: 

                1904    1895    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6277
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2025    2066    3602    1870 
   Size:         73      82      41    1536    4545

  Fragments arranged by size: 

                4545    1536      82      73      41
 
BamHI G'GATC_C

Cuts at:   3607    3974    3607 
   Size:        367    5910
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2953    3598    5053     618 
   Size:        325     217     627    1166     645    1455    1842

  Fragments arranged by size: 

                1842    1455    1166     645     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2872    3596    1391 
   Size:       1481     724    4072

  Fragments arranged by size: 

                4072    1481     724
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3070    3183    3250     928 
   Size:        971    1171     113      67    3955

  Fragments arranged by size: 

                3955    1171     971     113      67
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4468    4502    1903 
   Size:         34    2531      34    3678

  Fragments arranged by size: 

                3678    2531      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2142    4169    5379    2142 
   Size:       2027    1210    3040

  Fragments arranged by size: 

                3040    2027    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6277
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4888     136 
   Size:        107     122      71     631     605    3216    1525

  Fragments arranged by size: 

                3216    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6272    6272 
   Size:       6277
 
BlpI GC'TnA_GC

Cuts at:   3694    3694 
   Size:       6277
 
Bme1580I G_kGCm'C

Cuts at:   1790    4338    5494    1790 
   Size:       2548    1156    2573

  Fragments arranged by size: 

                2573    2548    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6277
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4008    4966     442 
   Size:        416      53      83     399      24    2591     958    1753

  Fragments arranged by size: 

                2591    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6277
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3366    4937    1239 
   Size:       2127    1571    2579

  Fragments arranged by size: 

                2579    2127    1571
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2865    3284    4402    5267    5565    2865 
   Size:        419    1118     865     298    3577

  Fragments arranged by size: 

                3577    1118     865     419     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4940     882 
   Size:        617    3441    2219

  Fragments arranged by size: 

                3441    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3084    4068     493 
   Size:        711    1880     984    2702

  Fragments arranged by size: 

                2702    1880     984     711
 
BsaBI GATnn'nnATC

Cuts at:   6271    6271 
   Size:       6277
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4084    4466    5101     275 
   Size:         53      83     186     936    2551     382     635    1451

  Fragments arranged by size: 

                2551    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4710    5108    5382    5529    5940    4710 
   Size:        398     274     147     411    5047

  Fragments arranged by size: 

                5047     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3096    3126    1178 
   Size:         30     165      30    1693      30    4329

  Fragments arranged by size: 

                4329    1693     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3290    1186 
   Size:       2104    4173
 
BseYI C'CCAG_C

Cuts at:   5480    5976    5480 
   Size:        496    5781
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2016    2580    1163 
   Size:        573     280     564    4860

  Fragments arranged by size: 

                4860     573     564     280
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3727    4488    5516    1540 
   Size:        275     186    1726     761    1028    2301

  Fragments arranged by size: 

                2301    1726    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2872    4338    4423    5494    2872 
   Size:       1466      85    1071    3655

  Fragments arranged by size: 

                3655    1466    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2492    3483    3788    3881    2492 
   Size:        991     305      93    4888

  Fragments arranged by size: 

                4888     991     305      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2872    3596    4338    4423    5494    1391 
   Size:        399    1082     724     742      85    1071    2174

  Fragments arranged by size: 

                2174    1082    1071     742     724     399      85
 
BspEI T'CCGG_A

Cuts at:   5940    5940 
   Size:       6277
 
BspHI T'CATG_A

Cuts at:   2872    4166    5896    2872 
   Size:       1294    1730    3253

  Fragments arranged by size: 

                3253    1730    1294
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2362    2704    3632     844 
   Size:       1518     342     928    3489

  Fragments arranged by size: 

                3489    1518     928     342
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4164    1394 
   Size:        429    2341    3507

  Fragments arranged by size: 

                3507    2341     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4772    4946      65 
   Size:       1183    3524     174    1396

  Fragments arranged by size: 

                3524    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2020    4921    5108    1700 
   Size:        110     210    2901     187    2869

  Fragments arranged by size: 

                2901    2869     210     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2901    3499      96 
   Size:       2805     598    2874

  Fragments arranged by size: 

                2874    2805     598
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6277
 
BssSI C'ACGA_G

Cuts at:   4337    5349    4337 
   Size:       1012    5265
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3361     794 
   Size:       2567    3710
 
BstBI TT'CG_AA

Cuts at:   5081    5081 
   Size:       6277
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6277
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3188    3188 
   Size:       6277
 
BstYI r'GATC_y

Cuts at:   1972    3607    3974    4359    4376    5828    5914    5926    6272 
   Size:       1635     367     385      17    1452      86      12     346

Cuts at:   6272    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1635    1452     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2305    3550     513 
   Size:        552     939     301    1245    3240

  Fragments arranged by size: 

                3240    1245     939     552     301
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2096     487 
   Size:        683      56     870    4668

  Fragments arranged by size: 

                4668     870     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2829    3793    4587    4607    2829 
   Size:        964     794      20    4499

  Fragments arranged by size: 

                4499     964     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2858    1554 
   Size:       1304    4973
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6242
 
DraI TTT'AAA

Cuts at:   2604    3581    2604 
   Size:        977    5300
 
DraIII CAC_nnn'GTG

Cuts at:   2182    2292    2182 
   Size:        110    6167
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2081    5284     809 
   Size:        507     765    3203    1802

  Fragments arranged by size: 

                3203    1802     765     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2007    2308    3724 
   Size:         54    1425      50     275      18     177     301    1416

Cuts at:   3724    4613    6242       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425    1416     889     301     275     177      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3724    1537 
   Size:        275    1912    4090

  Fragments arranged by size: 

                4090    1912     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3438    4207    1353 
   Size:       2085     769    3423

  Fragments arranged by size: 

                3423    2085     769
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4864    5238    5384     387 
   Size:       1064     138    3275     374     146    1280

  Fragments arranged by size: 

                3275    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2870    2870 
   Size:       6277
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2145    2189    2459    2760    3189    3366    4340 
   Size:        393     513      44     270     301     429     177     974

Cuts at:   4340    4937    5724    5992    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524     974     787     597     513     429     393     301
                 270     268     177      44
 
EcoO109I rG'GnC_Cy

Cuts at:   3304    3304 
   Size:       6277
 
EcoRI G'AATT_C

Cuts at:   3586    3964    3586 
   Size:        378    5899
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2194    2194 
   Size:       6277
 
FspI TGC'GCA

Cuts at:   4783    4783 
   Size:       6277
 
HaeII r_GCGC'y

Cuts at:   1678    5424    1678 
   Size:       3746    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4918    4950    4992    5024    1373 
   Size:         32     135      32    3346      32      42      32    2626

  Fragments arranged by size: 

                3346    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3621    3867     669 
   Size:        988     281    1683     246    3079

  Fragments arranged by size: 

                3079    1683     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2559    2571     748 
   Size:       1811      12    4454

  Fragments arranged by size: 

                4454    1811      12
 
HpaI GTT'AAC

Cuts at:   3867    3867 
   Size:       6277
 
KpnI G_GTAC'C

Cuts at:   3602    3602 
   Size:       6277
 
MfeI C'AATT_G

Cuts at:   3237    3876    3237 
   Size:        639    5638
 
MluI A'CGCG_T

Cuts at:   6153    6153 
   Size:       6277
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3082    5391    5575    1305 
   Size:       1777    2309     184    2007

  Fragments arranged by size: 

                2309    2007    1777     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2310      10 
   Size:         54    1425     343     478    3977

  Fragments arranged by size: 

                3977    1425     478     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3320    4235    4594    4753     518 
   Size:        648    2154     915     359     159    2042

  Fragments arranged by size: 

                2154    2042     915     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3197    3693    4370    5518    5763    1606 
   Size:       1591     496     677    1148     245    2120

  Fragments arranged by size: 

                2120    1591    1148     677     496     245
 
NaeI GCC'GGC

Cuts at:   2022    2022 
   Size:       6277
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2305     513 
   Size:        552     939     301    4485

  Fragments arranged by size: 

                4485     939     552     301
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6277
 
NgoMIV G'CCGG_C

Cuts at:   2020    2020 
   Size:       6277
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6277
 
NotI GC'GGCC_GC

Cuts at:   3724    3724 
   Size:       6277
 
NspI r_CATG'y

Cuts at:   1132    1229    3539    3635    5180    1132 
   Size:         97    2310      96    1545    2229

  Fragments arranged by size: 

                2310    2229    1545      97      96
 
PasI CC'CwG_GG

Cuts at:   1348    2154    1348 
   Size:        806    5471
 
PciI A'CATG_T

Cuts at:   5176    5176 
   Size:       6277
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6277
 
PmeI GTTT'AAAC

Cuts at:   3581    3581 
   Size:       6277
 
PmlI CAC'GTG

Cuts at:   3084    3084 
   Size:       6277
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4346    4378    5859    5891    4346 
   Size:         32    1481      32    4732

  Fragments arranged by size: 

                4732    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6277
 
PsiI TTA'TAA

Cuts at:   2811    3847    2811 
   Size:       1036    5241
 
PstI C_TGCA'G

Cuts at:    830    3202    3437    3629     830 
   Size:       2372     235     192    3478

  Fragments arranged by size: 

                3478    2372     235     192
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6277
 
PvuII CAG'CTG

Cuts at:   3197    3197 
   Size:       6277
 
SacI G_AGCT'C

Cuts at:   2872    2872 
   Size:       6277
 
SalI G'TCGA_C

Cuts at:   1936    3619    1936 
   Size:       1683    4594
 
SbfI CC_TGCA'GG

Cuts at:   3629    3629 
   Size:       6277
 
ScaI AGT'ACT

Cuts at:   4525    6237    4525 
   Size:       1712    4565
 
SfcI C'TryA_G

Cuts at:    826    1045    2893    2919    3198    3433    3625    4760    5441 
   Size:        219    1848      26     279     235     192    1135     681

Cuts at:   5441    5632     826 
   Size:        191    1471

  Fragments arranged by size: 

                1848    1471    1135     681     279     235     219     192
                 191      26
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6277
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6277
 
SmaI CCC'GGG

Cuts at:   1872    2027    2068    3604    1872 
   Size:        155      41    1536    4545

  Fragments arranged by size: 

                4545    1536     155      41
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2880    3299    4381    5282    5544     820 
   Size:        197     926     937     419    1082     901     262    1553

  Fragments arranged by size: 

                1553    1082     937     926     901     419     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4068     493 
   Size:       3575    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6277
 
SphI G_CATG'C

Cuts at:   1132    3635    1132 
   Size:       2503    3774
 
SrfI GCCC'GGGC

Cuts at:   2027    2027 
   Size:       6277
 
SspI AAT'ATT

Cuts at:      5      52    2507    3022    4201       5 
   Size:         47    2455     515    1179    2081

  Fragments arranged by size: 

                2455    2081    1179     515      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6277
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2305    3716     513 
   Size:        552     939     301    1411    3074

  Fragments arranged by size: 

                3074    1411     939     552     301
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4304    4321    4474    4659    5104     325 
   Size:       1255    2724      17     153     185     445    1498

  Fragments arranged by size: 

                2724    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2901    3499    4523 
   Size:        274      80      33      51    1443     924     598    1024

Cuts at:   4523    6235      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1024     924     598     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3096    3128    6237    6269    3096 
   Size:         32    3109      32    3104

  Fragments arranged by size: 

                3109    3104      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2078    1316 
   Size:        762    5515
 
XbaI T'CTAG_A

Cuts at:   3613    3613 
   Size:       6277
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3051    3051 
   Size:       6277
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6277
 
XmaI C'CCGG_G

Cuts at:   1870    2025    2066    3602    1870 
   Size:        155      41    1536    4545

  Fragments arranged by size: 

                4545    1536     155      41
 
XmnI GAAnn'nnTTC

Cuts at:   4406    4406 
   Size:       6277
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4085     276 
   Size:         53      83     186     936    2551    2468

  Fragments arranged by size: 

                2551    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI     PstI     PvuI    PvuII     SacI     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I    EcoNI
    EcoRV     FseI    FspAI     KasI     NarI     NruI     NsiI     PacI
     PfoI    PpuMI   PspOMI    PspXI     PsrI    RsrII    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SwaI
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