Restriction map for pFN21AB4148
 (Linear) (Six-base) MAPSORT of: KIBB4148.seq  Check: 3213  from: 1  to: 1572



KIBB4148.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     357     699    1572 
   Size:        357     342     873

  Fragments arranged by size: 

                 873     357     342
 
AccI GT'mk_AC

Cuts at:      0     968    1572 
   Size:        968     604
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     184     454     755    1184    1572 
   Size:        140      44     270     301     429     388

  Fragments arranged by size: 

                 429     388     301     270     140      44
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1356    1572 
   Size:       1356     216
 
ApoI r'AATT_y

Cuts at:      0     826    1223    1572 
   Size:        826     397     349

  Fragments arranged by size: 

                 826     397     349
 
AseI AT'TA_AT

Cuts at:      0     931    1572 
   Size:        931     641
 
AvaI C'yCGr_G

Cuts at:      0      20      61    1572 
   Size:         20      41    1511

  Fragments arranged by size: 

                1511      41      20
 
BanI G'GyrC_C

Cuts at:      0     948    1572 
   Size:        948     624
 
BanII G_rGCy'C

Cuts at:      0     867    1572 
   Size:        867     705
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1065    1178    1245    1572 
   Size:       1065     113      67     327

  Fragments arranged by size: 

                1065     327     113      67
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     137    1572 
   Size:        137    1435
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1361    1572 
   Size:       1361     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     860    1279    1572 
   Size:        860     419     293

  Fragments arranged by size: 

                 860     419     293
 
BsaAI yAC'GTr

Cuts at:      0    1079    1572 
   Size:       1079     493
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1091    1121    1572 
   Size:       1091      30     451

  Fragments arranged by size: 

                1091     451      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1285    1572 
   Size:       1285     287
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     575    1572 
   Size:         11     564     997

  Fragments arranged by size: 

                 997     564      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     867    1572 
   Size:        867     705
 
BsmI GAATG_Cn'

Cuts at:      0     487    1478    1572 
   Size:        487     991      94

  Fragments arranged by size: 

                 991     487      94
 
Bsp1286I G_dGCh'C

Cuts at:      0     867    1572 
   Size:        867     705
 
BspHI T'CATG_A

Cuts at:      0     867    1572 
   Size:        867     705
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     357     699    1572 
   Size:        357     342     873

  Fragments arranged by size: 

                 873     357     342
 
BsrFI r'CCGG_y

Cuts at:      0      15    1572 
   Size:         15    1557
 
BsrGI T'GTAC_A

Cuts at:      0     896    1494    1572 
   Size:        896     598      78

  Fragments arranged by size: 

                 896     598      78
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1356    1572 
   Size:       1356     216
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1183    1572 
   Size:       1183     389
 
BtgI C'CryG_G

Cuts at:      0     300    1545    1572 
   Size:        300    1245      27

  Fragments arranged by size: 

                1245     300      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      91    1572 
   Size:         91    1481
 
BtsI GCAGTG_nn'

Cuts at:      0     824    1572 
   Size:        824     748
 
ClaI AT'CG_AT

Cuts at:      0     853    1572 
   Size:        853     719
 
DraI TTT'AAA

Cuts at:      0     599    1572 
   Size:        599     973
 
DraIII CAC_nnn'GTG

Cuts at:      0     177     287    1572 
   Size:        177     110    1285

  Fragments arranged by size: 

                1285     177     110
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      76    1572 
   Size:         76    1496
 
EaeI y'GGCC_r

Cuts at:      0       2     303    1572 
   Size:          2     301    1269

  Fragments arranged by size: 

                1269     301       2
 
EarI CTCTTCn'nnn_

Cuts at:      0    1433    1572 
   Size:       1433     139
 
EcoICRI GAG'CTC

Cuts at:      0     865    1572 
   Size:        865     707
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     184     454     755    1184    1361    1572 
   Size:        140      44     270     301     429     177     211

  Fragments arranged by size: 

                 429     301     270     211     177     140      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1299    1572 
   Size:       1299     273
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     189    1572 
   Size:        189    1383
 
HindIII A'AGCT_T

Cuts at:      0     554     566    1572 
   Size:        554      12    1006

  Fragments arranged by size: 

                1006     554      12
 
MfeI C'AATT_G

Cuts at:      0    1232    1572 
   Size:       1232     340
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1077    1572 
   Size:       1077     495
 
MscI TGG'CCA

Cuts at:      0     305    1572 
   Size:        305    1267
 
MslI CAynn'nnrTG

Cuts at:      0    1315    1572 
   Size:       1315     257
 
MspA1I CmG'CkG

Cuts at:      0    1192    1572 
   Size:       1192     380
 
NaeI GCC'GGC

Cuts at:      0      17    1572 
   Size:         17    1555
 
NcoI C'CATG_G

Cuts at:      0     300    1572 
   Size:        300    1272
 
NgoMIV G'CCGG_C

Cuts at:      0      15    1572 
   Size:         15    1557
 
NspI r_CATG'y

Cuts at:      0    1534    1572 
   Size:       1534      38
 
PasI CC'CwG_GG

Cuts at:      0     149    1572 
   Size:        149    1423
 
PmlI CAC'GTG

Cuts at:      0    1079    1572 
   Size:       1079     493
 
PsiI TTA'TAA

Cuts at:      0     806    1572 
   Size:        806     766
 
PstI C_TGCA'G

Cuts at:      0    1197    1432    1572 
   Size:       1197     235     140

  Fragments arranged by size: 

                1197     235     140
 
PvuII CAG'CTG

Cuts at:      0    1192    1572 
   Size:       1192     380
 
SacI G_AGCT'C

Cuts at:      0     867    1572 
   Size:        867     705
 
SfcI C'TryA_G

Cuts at:      0     888     914    1193    1428    1572 
   Size:        888      26     279     235     144

  Fragments arranged by size: 

                 888     279     235     144      26
 
SmaI CCC'GGG

Cuts at:      0      22      63    1572 
   Size:         22      41    1509

  Fragments arranged by size: 

                1509      41      22
 
SmlI C'TyrA_G

Cuts at:      0     875    1294    1572 
   Size:        875     419     278

  Fragments arranged by size: 

                 875     419     278
 
SrfI GCCC'GGGC

Cuts at:      0      22    1572 
   Size:         22    1550
 
SspI AAT'ATT

Cuts at:      0     502    1017    1572 
   Size:        502     515     555

  Fragments arranged by size: 

                 555     515     502
 
StyI C'CwwG_G

Cuts at:      0     300    1572 
   Size:        300    1272
 
TatI w'GTAC_w

Cuts at:      0     896    1494    1572 
   Size:        896     598      78

  Fragments arranged by size: 

                 896     598      78
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1091    1123    1572 
   Size:       1091      32     449

  Fragments arranged by size: 

                1091     449      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      73    1572 
   Size:         73    1499
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1046    1572 
   Size:       1046     526
 
XmaI C'CCGG_G

Cuts at:      0      20      61    1572 
   Size:         20      41    1511

  Fragments arranged by size: 

                1511      41      20
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AlwNI     ApoI     AseI     AvaI     BanI
    BanII     BbsI    BciVI     BpmI    BpuEI    BsaAI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrFI    BsrGI
   BstAPI    BstXI     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI  HindIII
     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NspI     PasI     PmlI     PsiI     PstI    PvuII     SacI     SfcI
     SmaI     SmlI     SrfI     SspI     StyI     TatI     TstI  Tth111I
     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BsrBI    BsrDI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    CspCI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     StuI     SwaI    TaqII     XbaI     XhoI
     XmnI     ZraI
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