(Circular) (Six-base) MAPSORT of: pFN21AB3238.seq Check: 4779 from: 1 to: 6901 pFN21AB3238 6901 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4711 278 Size: 53 83 186 936 3175 2468 Fragments arranged by size: 3175 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4244 1937 Size: 2307 4594 Acc65I G'GTAC_C Cuts at: 4222 4222 Size: 6901 AclI AA'CG_TT Cuts at: 1563 5028 5401 1563 Size: 3465 373 3063 Fragments arranged by size: 3465 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2934 3715 3771 4964 6348 6616 1632 Size: 1302 781 56 1193 1384 268 1917 Fragments arranged by size: 1917 1384 1302 1193 781 268 56 AfeI AGC'GCT Cuts at: 3965 3965 Size: 6901 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6704 AflIII A'CryG_T Cuts at: 1203 3303 5800 6777 1203 Size: 2100 2497 977 1327 Fragments arranged by size: 2497 2100 1327 977 AgeI A'CCGG_T Cuts at: 5732 5732 Size: 6901 AhdI GACnn_n'nnGTC Cuts at: 5630 5630 Size: 6901 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6901 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6901 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3069 3101 3069 Size: 32 6869 AlwNI CAG_nnn'CTG Cuts at: 3484 4154 4644 6216 3484 Size: 670 490 1572 4169 Fragments arranged by size: 4169 1572 670 490 ApaLI G'TGCA_C Cuts at: 2240 4958 6114 2240 Size: 2718 1156 3027 Fragments arranged by size: 3027 2718 1156 ApoI r'AATT_y Cuts at: 1493 2673 3660 4210 4436 4588 6697 1493 Size: 1180 987 550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1180 987 550 226 152 AseI AT'TA_AT Cuts at: 160 1032 5455 160 Size: 872 4423 1606 Fragments arranged by size: 4423 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 AvaI C'yCGr_G Cuts at: 1870 1943 3507 4226 1870 Size: 73 1564 719 4545 Fragments arranged by size: 4545 1564 719 73 BamHI G'GATC_C Cuts at: 4231 4598 4231 Size: 367 6534 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4222 5677 618 Size: 325 217 627 2435 1455 1842 Fragments arranged by size: 2435 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3727 4220 1391 Size: 2336 493 4072 Fragments arranged by size: 4072 2336 493 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2704 3294 3416 3432 928 Size: 971 805 590 122 16 4397 Fragments arranged by size: 4397 971 805 590 122 16 BbvCI CC'TCA_GC Cuts at: 2417 3564 4098 2417 Size: 1147 534 5220 Fragments arranged by size: 5220 1147 534 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5092 5126 1903 Size: 34 3155 34 3678 Fragments arranged by size: 3678 3155 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3871 4793 6003 3871 Size: 922 1210 4769 Fragments arranged by size: 4769 1210 922 BclI T'GATC_A Cuts at: 1548 4059 1548 Size: 2511 4390 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5512 136 Size: 107 122 71 631 605 3840 1525 Fragments arranged by size: 3840 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6896 6896 Size: 6901 BlpI GC'TnA_GC Cuts at: 4318 4318 Size: 6901 Bme1580I G_kGCm'C Cuts at: 1790 2244 4962 6118 1790 Size: 454 2718 1156 2573 Fragments arranged by size: 2718 2573 1156 454 BmgBI CAC'GTC Cuts at: 1318 2050 1318 Size: 732 6169 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4632 5590 442 Size: 416 53 83 399 24 3215 958 1753 Fragments arranged by size: 3215 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6901 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4093 4093 Size: 6901 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 3724 5561 1239 Size: 1058 1427 1837 2579 Fragments arranged by size: 2579 1837 1427 1058 Bpu10I CC'TnA_GC Cuts at: 2417 2557 3564 3654 4098 2417 Size: 140 1007 90 444 5220 Fragments arranged by size: 5220 1007 444 140 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2639 3198 5026 5891 6189 2639 Size: 559 1828 865 298 3351 Fragments arranged by size: 3351 1828 865 559 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5564 882 Size: 617 4065 2219 Fragments arranged by size: 4065 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4692 493 Size: 711 3488 2702 Fragments arranged by size: 3488 2702 711 BsaBI GATnn'nnATC Cuts at: 2223 6895 2223 Size: 4672 2229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4708 5090 5725 275 Size: 53 83 186 936 3175 382 635 1451 Fragments arranged by size: 3175 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5334 5732 6006 6153 6564 5334 Size: 398 274 147 411 5671 Fragments arranged by size: 5671 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3002 3032 1178 Size: 30 165 30 1599 30 5047 Fragments arranged by size: 5047 1599 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2553 1186 Size: 1367 5534 BseYI C'CCAG_C Cuts at: 6104 6600 6104 Size: 496 6405 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3290 3473 1163 Size: 573 1554 183 4591 Fragments arranged by size: 4591 1554 573 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2015 4351 5112 6140 1540 Size: 275 186 14 2336 761 1028 2301 Fragments arranged by size: 2336 2301 1028 761 275 186 14 BsiHKAI G_wGCw'C Cuts at: 2244 4962 5047 6118 2244 Size: 2718 85 1071 3027 Fragments arranged by size: 3027 2718 1071 85 BsmI GAATG_Cn' Cuts at: 2731 3364 4412 4505 2731 Size: 633 1048 93 5127 Fragments arranged by size: 5127 1048 633 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3742 3742 Size: 6901 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 3727 4220 4962 5047 6118 1391 Size: 399 454 1483 493 742 85 1071 2174 Fragments arranged by size: 2174 1483 1071 742 493 454 399 85 BspEI T'CCGG_A Cuts at: 6564 6564 Size: 6901 BspHI T'CATG_A Cuts at: 4790 6520 4790 Size: 1730 5171 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4256 844 Size: 3412 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4788 1394 Size: 429 2965 3507 Fragments arranged by size: 3507 2965 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5396 5570 65 Size: 1183 4148 174 1396 Fragments arranged by size: 4148 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5545 5732 1700 Size: 110 3735 187 2869 Fragments arranged by size: 3735 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2128 96 Size: 2032 4869 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6901 BssSI C'ACGA_G Cuts at: 4961 5973 4961 Size: 1012 5889 BstAPI GCAn_nnn'nTGC Cuts at: 794 3915 794 Size: 3121 3780 BstBI TT'CG_AA Cuts at: 5705 5705 Size: 6901 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6901 BstXI CCAn_nnnn'nTGG Cuts at: 3391 3683 3391 Size: 292 6609 BstYI r'GATC_y Cuts at: 1972 4231 4598 4983 5000 6452 6538 6550 6896 Size: 2259 367 385 17 1452 86 12 346 Cuts at: 6896 1972 Size: 1977 Fragments arranged by size: 2259 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2566 513 Size: 552 939 562 4848 Fragments arranged by size: 4848 939 562 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6162 Fragments arranged by size: 6162 683 56 BtsI GCAGTG_nn' Cuts at: 3763 4417 5211 5231 3763 Size: 654 794 20 5433 Fragments arranged by size: 5433 794 654 20 ClaI AT'CG_AT Cuts at: 1554 2801 1554 Size: 1247 5654 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4120 4155 529 Size: 35 3556 35 3275 Fragments arranged by size: 3556 3275 35 35 DraI TTT'AAA Cuts at: 2922 4205 2922 Size: 1283 5618 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5908 809 Size: 507 4592 1802 Fragments arranged by size: 4592 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2563 3174 4348 Size: 54 1425 50 275 18 733 611 1174 Cuts at: 4348 5237 6866 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1174 889 733 611 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4348 1537 Size: 275 2536 4090 Fragments arranged by size: 4090 2536 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 3899 3951 4831 1353 Size: 1031 1515 52 880 3423 Fragments arranged by size: 3423 1515 1031 880 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5488 5862 6008 387 Size: 1064 138 3899 374 146 1280 Fragments arranged by size: 3899 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2934 3715 3724 3771 4964 5561 Size: 393 665 637 781 9 47 1193 597 Cuts at: 5561 6348 6616 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1193 787 781 665 637 597 393 268 47 9 EcoRI G'AATT_C Cuts at: 4210 4588 4210 Size: 378 6523 EcoRV GAT'ATC Cuts at: 2643 2643 Size: 6901 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2633 3844 2633 Size: 1211 5690 FspI TGC'GCA Cuts at: 5407 5407 Size: 6901 HaeII r_GCGC'y Cuts at: 1678 3967 6048 1678 Size: 2289 2081 2531 Fragments arranged by size: 2531 2289 2081 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5542 5574 5616 5648 1373 Size: 32 135 32 3970 32 42 32 2626 Fragments arranged by size: 3970 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4245 4491 669 Size: 988 281 2307 246 3079 Fragments arranged by size: 3079 2307 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6901 HpaI GTT'AAC Cuts at: 4491 4491 Size: 6901 KpnI G_GTAC'C Cuts at: 4226 4226 Size: 6901 MfeI C'AATT_G Cuts at: 4500 4500 Size: 6901 MluI A'CGCG_T Cuts at: 6777 6777 Size: 6901 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2323 2646 3461 3751 6015 6199 1305 Size: 1018 323 815 290 2264 184 2007 Fragments arranged by size: 2264 2007 1018 815 323 290 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2565 3176 10 Size: 54 1425 343 733 611 3735 Fragments arranged by size: 3735 1425 733 611 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2237 2351 3249 3681 4859 5218 5377 Size: 648 1071 114 898 432 1178 359 159 Cuts at: 5377 518 Size: 2042 Fragments arranged by size: 2042 1178 1071 898 648 432 359 159 114 MspA1I CmG'CkG Cuts at: 1606 2030 2443 3122 3274 3563 4097 4154 4317 Size: 424 413 679 152 289 534 57 163 Cuts at: 4317 4994 6142 6387 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 679 677 534 424 413 289 245 163 152 57 NcoI C'CATG_G Cuts at: 513 1065 2004 2566 513 Size: 552 939 562 4848 Fragments arranged by size: 4848 939 562 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6901 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6901 NotI GC'GGCC_GC Cuts at: 4348 4348 Size: 6901 NspI r_CATG'y Cuts at: 1132 1229 3307 4259 5804 1132 Size: 97 2078 952 1545 2229 Fragments arranged by size: 2229 2078 1545 952 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6901 PciI A'CATG_T Cuts at: 3303 5800 3303 Size: 2497 4404 PflMI CCAn_nnn'nTGG Cuts at: 1108 2742 1108 Size: 1634 5267 PfoI T'CCnGG_A Cuts at: 2325 3930 4041 2325 Size: 1605 111 5185 Fragments arranged by size: 5185 1605 111 PmeI GTTT'AAAC Cuts at: 4205 4205 Size: 6901 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4970 5002 6483 6515 4970 Size: 32 1481 32 5356 Fragments arranged by size: 5356 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6901 PsiI TTA'TAA Cuts at: 2453 4471 2453 Size: 2018 4883 PstI C_TGCA'G Cuts at: 830 3127 4253 830 Size: 2297 1126 3478 Fragments arranged by size: 3478 2297 1126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6901 PvuII CAG'CTG Cuts at: 2030 2443 3122 3274 4097 4154 2030 Size: 413 679 152 823 57 4777 Fragments arranged by size: 4777 823 679 413 152 57 SalI G'TCGA_C Cuts at: 1936 4243 1936 Size: 2307 4594 SapI GCTCTTCn'nnn_ Cuts at: 3899 3951 3899 Size: 52 6849 SbfI CC_TGCA'GG Cuts at: 4253 4253 Size: 6901 ScaI AGT'ACT Cuts at: 5149 6861 5149 Size: 1712 5189 SexAI A'CCwGG_T Cuts at: 2302 2302 Size: 6901 SfcI C'TryA_G Cuts at: 826 1045 2807 3123 3690 4249 5384 6065 6256 Size: 219 1762 316 567 559 1135 681 191 Cuts at: 6256 826 Size: 1471 Fragments arranged by size: 1762 1471 1135 681 567 559 316 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6901 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6901 SmaI CCC'GGG Cuts at: 1872 3509 4228 1872 Size: 1637 719 4545 Fragments arranged by size: 4545 1637 719 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 3213 5005 5906 6168 820 Size: 197 926 675 595 1792 901 262 1553 Fragments arranged by size: 1792 1553 926 901 675 595 262 197 SnaBI TAC'GTA Cuts at: 493 4692 493 Size: 4199 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6901 SphI G_CATG'C Cuts at: 1132 4259 1132 Size: 3127 3774 SspI AAT'ATT Cuts at: 5 52 2406 4825 5 Size: 47 2354 2419 2081 Fragments arranged by size: 2419 2354 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6901 StyI C'CwwG_G Cuts at: 513 1065 2004 2566 4340 513 Size: 552 939 562 1774 3074 Fragments arranged by size: 3074 1774 939 562 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4928 4945 5098 5283 5728 325 Size: 1255 3348 17 153 185 445 1498 Fragments arranged by size: 3348 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2128 5147 6859 Size: 274 80 33 51 1443 151 3019 1712 Cuts at: 6859 96 Size: 138 Fragments arranged by size: 3019 1712 1443 274 151 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2185 2217 3002 3034 6861 6893 2185 Size: 32 785 32 3827 32 2193 Fragments arranged by size: 3827 2193 785 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6901 XbaI T'CTAG_A Cuts at: 4237 4237 Size: 6901 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4114 4114 Size: 6901 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6901 XmaI C'CCGG_G Cuts at: 1870 3507 4226 1870 Size: 1637 719 4545 Fragments arranged by size: 4545 1637 719 XmnI GAAnn'nnTTC Cuts at: 3020 5030 3020 Size: 2010 4891 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4709 276 Size: 53 83 186 936 3175 2468 Fragments arranged by size: 3175 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI