Restriction map for pFN21AB8135
 (Linear) (Six-base) MAPSORT of: KIBB8135.seq  Check: 9704  from: 1  to: 1491



KIBB8135.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AatII G_ACGT'C

Cuts at:      0     905    1491 
   Size:        905     586
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184    1225    1491 
   Size:        184    1041     266

  Fragments arranged by size: 

                1041     266     184
 
AflII C'TTAA_G

Cuts at:      0     663    1491 
   Size:        663     828
 
AflIII A'CryG_T

Cuts at:      0     174    1491 
   Size:        174    1317
 
AhdI GACnn_n'nnGTC

Cuts at:      0     849    1491 
   Size:        849     642
 
AleI CACnn'nnGTG

Cuts at:      0     145    1491 
   Size:        145    1346
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     145     177    1491 
   Size:        145      32    1314

  Fragments arranged by size: 

                1314     145      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1021    1203    1429    1491 
   Size:       1021     182     226      62

  Fragments arranged by size: 

                1021     226     182      62
 
ApaLI G'TGCA_C

Cuts at:      0    1338    1491 
   Size:       1338     153
 
AvaI C'yCGr_G

Cuts at:      0      13     807     825    1491 
   Size:         13     794      18     666

  Fragments arranged by size: 

                 794     666      18      13
 
BanI G'GyrC_C

Cuts at:      0      31     413    1104    1491 
   Size:         31     382     691     387

  Fragments arranged by size: 

                 691     387     382      31
 
BanII G_rGCy'C

Cuts at:      0      14    1491 
   Size:         14    1477
 
BbeI G_GCGC'C

Cuts at:      0      35    1491 
   Size:         35    1456
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     126     897    1213    1491 
   Size:        126     771     316     278

  Fragments arranged by size: 

                 771     316     278     126
 
BglI GCCn_nnn'nGGC

Cuts at:      0     781    1491 
   Size:        781     710
 
Bme1580I G_kGCm'C

Cuts at:      0      20     834    1342    1491 
   Size:         20     814     508     149

  Fragments arranged by size: 

                 814     508     149      20
 
BmgBI CAC'GTC

Cuts at:      0     143    1491 
   Size:        143    1348
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     384     667    1491 
   Size:        384     283     824

  Fragments arranged by size: 

                 824     384     283
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1150    1491 
   Size:       1150     341
 
Bpu10I CC'TnA_GC

Cuts at:      0     456     965    1491 
   Size:        456     509     526

  Fragments arranged by size: 

                 526     509     456
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     265    1491 
   Size:        265    1226
 
BsaAI yAC'GTr

Cuts at:      0    1458    1491 
   Size:       1458      33
 
BsaHI Gr'CG_yC

Cuts at:      0      32     902    1491 
   Size:         32     870     589

  Fragments arranged by size: 

                 870     589      32
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     481     511    1351    1381    1491 
   Size:        481      30     840      30     110

  Fragments arranged by size: 

                 840     481     110      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     314    1491 
   Size:        314    1177
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     958    1491 
   Size:        958     533
 
BsiEI CG_ry'CG

Cuts at:      0     465    1320    1491 
   Size:        465     855     171

  Fragments arranged by size: 

                 855     465     171
 
BsiHKAI G_wGCw'C

Cuts at:      0    1342    1491 
   Size:       1342     149
 
BsiWI C'GTAC_G

Cuts at:      0     408    1491 
   Size:        408    1083
 
BsmI GAATG_Cn'

Cuts at:      0     560    1491 
   Size:        560     931
 
Bsp1286I G_dGCh'C

Cuts at:      0      14      20     834    1342    1491 
   Size:         14       6     814     508     149

  Fragments arranged by size: 

                 814     508     149      14       6
 
BsrBI CCG'CTC

Cuts at:      0    1290    1491 
   Size:       1290     201
 
BsrDI GCAATG_nn'

Cuts at:      0     763    1491 
   Size:        763     728
 
BsrFI r'CCGG_y

Cuts at:      0    1342    1491 
   Size:       1342     149
 
BsrGI T'GTAC_A

Cuts at:      0     437    1491 
   Size:        437    1054
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1346    1491 
   Size:       1346     145
 
BstEII G'GTnAC_C

Cuts at:      0      87    1482    1491 
   Size:         87    1395       9

  Fragments arranged by size: 

                1395      87       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147     256    1491 
   Size:        147     109    1235

  Fragments arranged by size: 

                1235     147     109
 
BstYI r'GATC_y

Cuts at:      0     605    1271    1491 
   Size:        605     666     220

  Fragments arranged by size: 

                 666     605     220
 
BtgI C'CryG_G

Cuts at:      0     521     779     854    1144    1491 
   Size:        521     258      75     290     347

  Fragments arranged by size: 

                 521     347     290     258      75
 
BtsI GCAGTG_nn'

Cuts at:      0     592     866    1491 
   Size:        592     274     625

  Fragments arranged by size: 

                 625     592     274
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     495     530    1491 
   Size:        495      35     961

  Fragments arranged by size: 

                 961     495      35
 
EaeI y'GGCC_r

Cuts at:      0     104     345     462    1317    1416    1491 
   Size:        104     241     117     855      99      75

  Fragments arranged by size: 

                 855     241     117     104      99      75
 
EagI C'GGCC_G

Cuts at:      0     462    1317    1491 
   Size:        462     855     174

  Fragments arranged by size: 

                 855     462     174
 
EarI CTCTTCn'nnn_

Cuts at:      0     525    1160    1491 
   Size:        525     635     331

  Fragments arranged by size: 

                 635     525     331
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      22    1491 
   Size:         22    1469
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184    1150    1225    1491 
   Size:        184     966      75     266

  Fragments arranged by size: 

                 966     266     184      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     595    1491 
   Size:        595     896
 
EcoO109I rG'GnC_Cy

Cuts at:      0      39     313     378    1491 
   Size:         39     274      65    1113

  Fragments arranged by size: 

                1113     274      65      39
 
HaeII r_GCGC'y

Cuts at:      0      35    1491 
   Size:         35    1456
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     461     493     835     867    1491 
   Size:        461      32     342      32     624

  Fragments arranged by size: 

                 624     461     342      32      32
 
HincII GTy'rAC

Cuts at:      0    1055    1176    1311    1359    1491 
   Size:       1055     121     135      48     132

  Fragments arranged by size: 

                1055     135     132     121      48
 
HpaI GTT'AAC

Cuts at:      0    1311    1491 
   Size:       1311     180
 
KasI G'GCGC_C

Cuts at:      0      31    1491 
   Size:         31    1460
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    1491 
   Size:        352    1139
 
MscI TGG'CCA

Cuts at:      0     106    1491 
   Size:        106    1385
 
MslI CAynn'nnrTG

Cuts at:      0      25     145     254    1491 
   Size:         25     120     109    1237

  Fragments arranged by size: 

                1237     120     109      25
 
MspA1I CmG'CkG

Cuts at:      0      67     428     781     995    1146    1203    1429    1491 
   Size:         67     361     353     214     151      57     226      62

  Fragments arranged by size: 

                 361     353     226     214     151      67      62      57
            
 
NarI GG'CG_CC

Cuts at:      0      32    1491 
   Size:         32    1459
 
NcoI C'CATG_G

Cuts at:      0     521    1491 
   Size:        521     970
 
NdeI CA'TA_TG

Cuts at:      0    1411    1491 
   Size:       1411      80
 
NspI r_CATG'y

Cuts at:      0     178    1234    1491 
   Size:        178    1056     257

  Fragments arranged by size: 

                1056     257     178
 
PasI CC'CwG_GG

Cuts at:      0     894    1491 
   Size:        894     597
 
PciI A'CATG_T

Cuts at:      0     174    1491 
   Size:        174    1317
 
PfoI T'CCnGG_A

Cuts at:      0     596    1491 
   Size:        596     895
 
PmlI CAC'GTG

Cuts at:      0    1458    1491 
   Size:       1458      33
 
PpuMI rG'GwC_Cy

Cuts at:      0     313     378    1491 
   Size:        313      65    1113

  Fragments arranged by size: 

                1113     313      65
 
PstI C_TGCA'G

Cuts at:      0     126    1491 
   Size:        126    1365
 
PvuII CAG'CTG

Cuts at:      0     995    1203    1429    1491 
   Size:        995     208     226      62

  Fragments arranged by size: 

                 995     226     208      62
 
SacII CC_GC'GG

Cuts at:      0     782    1147    1491 
   Size:        782     365     344

  Fragments arranged by size: 

                 782     365     344
 
SanDI GG'GwC_CC

Cuts at:      0     378    1491 
   Size:        378    1113
 
SapI GCTCTTCn'nnn_

Cuts at:      0     525    1491 
   Size:        525     966
 
SfcI C'TryA_G

Cuts at:      0     122    1491 
   Size:        122    1369
 
SfoI GGC'GCC

Cuts at:      0      33    1491 
   Size:         33    1458
 
SmaI CCC'GGG

Cuts at:      0      15    1491 
   Size:         15    1476
 
SmlI C'TyrA_G

Cuts at:      0     663    1491 
   Size:        663     828
 
SphI G_CATG'C

Cuts at:      0    1234    1491 
   Size:       1234     257
 
SrfI GCCC'GGGC

Cuts at:      0      15    1491 
   Size:         15    1476
 
StyI C'CwwG_G

Cuts at:      0     521    1491 
   Size:        521     970
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     860    1491 
   Size:        860     631
 
TatI w'GTAC_w

Cuts at:      0     437    1491 
   Size:        437    1054
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     297     329     753     785    1491 
   Size:        297      32     424      32     706

  Fragments arranged by size: 

                 706     424     297      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     130    1450    1491 
   Size:        130    1320      41

  Fragments arranged by size: 

                1320     130      41
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      99     355    1491 
   Size:         99     256    1136

  Fragments arranged by size: 

                1136     256      99
 
XmaI C'CCGG_G

Cuts at:      0      13    1491 
   Size:         13    1478
 
ZraI GAC'GTC

Cuts at:      0     903    1491 
   Size:        903     588
 

 Enzymes that do cut:

    AatII     AcuI    AflII   AflIII     AhdI     AleI     AloI    AlwNI
    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BglI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BsrBI
    BsrDI    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI     BtgI
     BtsI    CspCI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII     HpaI     KasI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NspI     PasI     PciI
     PfoI     PmlI    PpuMI     PstI    PvuII    SacII    SanDI     SapI
     SfcI     SfoI     SmaI     SmlI     SphI     SrfI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI     AgeI     AlfI     ApaI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BplI
    BpuEI    BsaBI    BsaWI    BseYI    BsmBI    BspEI    BspHI    BspMI
   BssHII    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     KpnI     MfeI     MluI     NaeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SspI     StuI     SwaI     XbaI     XhoI     XmnI
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