(Circular) (Six-base) MAPSORT of: pFN21AB9876.seq Check: 9379 from: 1 to: 6331 pFN21AB9876 6331 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3017 3017 Size: 6331 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4141 278 Size: 53 83 186 936 2605 2468 Fragments arranged by size: 2605 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3674 1937 Size: 1737 4594 Acc65I G'GTAC_C Cuts at: 2572 3652 2572 Size: 1080 5251 AclI AA'CG_TT Cuts at: 1563 4458 4831 1563 Size: 2895 373 3063 Fragments arranged by size: 3063 2895 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2157 2499 2580 3341 3477 4394 5778 6046 Size: 525 342 81 761 136 917 1384 268 Cuts at: 6046 1632 Size: 1917 Fragments arranged by size: 1917 1384 917 761 525 342 268 136 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6134 AflIII A'CryG_T Cuts at: 1203 2259 5230 6207 1203 Size: 1056 2971 977 1327 Fragments arranged by size: 2971 1327 1056 977 AgeI A'CCGG_T Cuts at: 5162 5162 Size: 6331 AhdI GACnn_n'nnGTC Cuts at: 2340 2442 3550 5060 2340 Size: 102 1108 1510 3611 Fragments arranged by size: 3611 1510 1108 102 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6331 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6331 AlwNI CAG_nnn'CTG Cuts at: 4074 5646 4074 Size: 1572 4759 ApaI G_GGCC'C Cuts at: 3090 3090 Size: 6331 ApaLI G'TGCA_C Cuts at: 4388 5544 4388 Size: 1156 5175 ApoI r'AATT_y Cuts at: 1493 2050 2164 2215 2966 3268 3640 3866 4018 Size: 557 114 51 751 302 372 226 152 Cuts at: 4018 6127 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 751 557 372 302 226 152 114 51 AseI AT'TA_AT Cuts at: 160 1032 4885 160 Size: 872 3853 1606 Fragments arranged by size: 3853 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 AvaI C'yCGr_G Cuts at: 1870 1943 3656 1870 Size: 73 1713 4545 Fragments arranged by size: 4545 1713 73 BamHI G'GATC_C Cuts at: 3661 4028 3661 Size: 367 5964 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2572 3652 5107 618 Size: 325 217 627 785 1080 1455 1842 Fragments arranged by size: 1842 1455 1080 785 627 325 217 BanII G_rGCy'C Cuts at: 1391 2390 3090 3650 1391 Size: 999 700 560 4072 Fragments arranged by size: 4072 999 700 560 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2352 2598 2888 3003 3546 3584 928 Size: 971 453 246 290 115 543 38 3675 Fragments arranged by size: 3675 971 543 453 290 246 115 38 BbvCI CC'TCA_GC Cuts at: 2906 2906 Size: 6331 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4522 4556 1903 Size: 34 2585 34 3678 Fragments arranged by size: 3678 2585 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4223 5433 4223 Size: 1210 5121 BclI T'GATC_A Cuts at: 1548 1548 Size: 6331 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4942 136 Size: 107 122 71 631 605 3270 1525 Fragments arranged by size: 3270 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2095 6326 2095 Size: 4231 2100 BlpI GC'TnA_GC Cuts at: 3748 3748 Size: 6331 Bme1580I G_kGCm'C Cuts at: 1790 3090 4392 5548 1790 Size: 1300 1302 1156 2573 Fragments arranged by size: 2573 1302 1300 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6331 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2403 4062 5020 Size: 416 53 83 399 24 986 1659 958 Cuts at: 5020 442 Size: 1753 Fragments arranged by size: 1753 1659 986 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2487 4991 1239 Size: 1248 2504 2579 Fragments arranged by size: 2579 2504 1248 Bpu10I CC'TnA_GC Cuts at: 2906 2906 Size: 6331 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4456 5321 5619 4456 Size: 865 298 5168 Fragments arranged by size: 5168 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2467 4994 882 Size: 617 968 2527 2219 Fragments arranged by size: 2527 2219 968 617 BsaAI yAC'GTr Cuts at: 493 1204 2260 4122 493 Size: 711 1056 1862 2702 Fragments arranged by size: 2702 1862 1056 711 BsaBI GATnn'nnATC Cuts at: 2088 2094 6325 2088 Size: 6 4231 2094 Fragments arranged by size: 4231 2094 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4138 4520 5155 275 Size: 53 83 186 936 2605 382 635 1451 Fragments arranged by size: 2605 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4764 5162 5436 5583 5994 4764 Size: 398 274 147 411 5101 Fragments arranged by size: 5101 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2445 2475 1178 Size: 30 165 30 1042 30 5034 Fragments arranged by size: 5034 1042 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2484 1186 Size: 904 394 5033 Fragments arranged by size: 5033 904 394 BseYI C'CCAG_C Cuts at: 2991 5534 6030 2991 Size: 2543 496 3292 Fragments arranged by size: 3292 2543 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5758 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2333 3781 4542 5570 1540 Size: 275 186 332 1448 761 1028 2301 Fragments arranged by size: 2301 1448 1028 761 332 275 186 BsiHKAI G_wGCw'C Cuts at: 2390 3411 4392 4477 5548 2390 Size: 1021 981 85 1071 3173 Fragments arranged by size: 3173 1071 1021 981 85 BsmI GAATG_Cn' Cuts at: 3842 3935 3842 Size: 93 6238 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2390 3090 3411 3650 4392 4477 5548 Size: 399 600 700 321 239 742 85 1071 Cuts at: 5548 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 700 600 399 321 239 85 BspEI T'CCGG_A Cuts at: 5994 5994 Size: 6331 BspHI T'CATG_A Cuts at: 4220 5950 4220 Size: 1730 4601 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3017 3686 844 Size: 2173 669 3489 Fragments arranged by size: 3489 2173 669 BsrBI CCG'CTC Cuts at: 1394 1823 4218 1394 Size: 429 2395 3507 Fragments arranged by size: 3507 2395 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2763 4826 5000 65 Size: 1183 1515 2063 174 1396 Fragments arranged by size: 2063 1515 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2308 3024 3372 4975 5162 1700 Size: 110 498 716 348 1603 187 2869 Fragments arranged by size: 2869 1603 716 498 348 187 110 BsrGI T'GTAC_A Cuts at: 96 2122 3070 96 Size: 2026 948 3357 Fragments arranged by size: 3357 2026 948 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6331 BssSI C'ACGA_G Cuts at: 3465 4391 5403 3465 Size: 926 1012 4393 Fragments arranged by size: 4393 1012 926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6331 BstBI TT'CG_AA Cuts at: 5135 5135 Size: 6331 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6331 BstYI r'GATC_y Cuts at: 1972 2089 2095 2640 2725 3661 4028 4413 4430 Size: 117 6 545 85 936 367 385 17 Cuts at: 4430 5882 5968 5980 6326 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 936 545 385 367 346 117 86 85 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 2004 3081 513 Size: 552 939 1077 3763 Fragments arranged by size: 3763 1077 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3061 487 Size: 683 56 1835 3757 Fragments arranged by size: 3757 1835 683 56 BtsI GCAGTG_nn' Cuts at: 3016 3847 4641 4661 3016 Size: 831 794 20 4686 Fragments arranged by size: 4686 831 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6331 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6296 DraI TTT'AAA Cuts at: 2899 3436 3635 2899 Size: 537 199 5595 Fragments arranged by size: 5595 537 199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5338 809 Size: 507 4022 1802 Fragments arranged by size: 4022 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3778 4667 6296 Size: 54 1425 50 275 18 1948 889 1629 Cuts at: 6296 8 Size: 43 Fragments arranged by size: 1948 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3778 1537 Size: 275 1966 4090 Fragments arranged by size: 4090 1966 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4261 1353 Size: 2908 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4918 5292 5438 387 Size: 1064 138 3329 374 146 1280 Fragments arranged by size: 3329 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2388 2388 Size: 6331 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 2487 2499 2580 3341 3477 4394 Size: 393 525 330 12 81 761 136 917 Cuts at: 4394 4991 5778 6046 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 917 787 761 597 525 393 330 268 136 81 12 EcoO109I rG'GnC_Cy Cuts at: 3086 3087 3136 3352 3086 Size: 1 49 216 6065 Fragments arranged by size: 6065 216 49 1 EcoRI G'AATT_C Cuts at: 3640 4018 3640 Size: 378 5953 EcoRV GAT'ATC Cuts at: 2014 2014 Size: 6331 FspI TGC'GCA Cuts at: 4837 4837 Size: 6331 HaeII r_GCGC'y Cuts at: 1678 5478 1678 Size: 3800 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2326 2347 2358 2428 Size: 32 135 32 743 11 21 11 70 Cuts at: 2428 2445 2460 2477 2785 2817 3201 3233 3536 Size: 17 15 17 308 32 384 32 303 Cuts at: 3536 3568 4972 5004 5046 5078 1373 Size: 32 1404 32 42 32 2626 Fragments arranged by size: 2626 1404 743 384 308 303 135 70 42 32 32 32 32 32 32 32 21 17 17 15 11 11 HincII GTy'rAC Cuts at: 669 1657 1938 3675 3921 669 Size: 988 281 1737 246 3079 Fragments arranged by size: 3079 1737 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6331 HpaI GTT'AAC Cuts at: 3921 3921 Size: 6331 KpnI G_GTAC'C Cuts at: 2576 3656 2576 Size: 1080 5251 MfeI C'AATT_G Cuts at: 3930 3930 Size: 6331 MluI A'CGCG_T Cuts at: 6207 6207 Size: 6331 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2431 3311 5445 5629 1305 Size: 1126 880 2134 184 2007 Fragments arranged by size: 2134 2007 1126 880 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4509 Fragments arranged by size: 4509 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2130 3080 4289 4648 4807 518 Size: 648 964 950 1209 359 159 2042 Fragments arranged by size: 2042 1209 964 950 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2910 2991 3747 4424 5572 5817 1606 Size: 1304 81 756 677 1148 245 2120 Fragments arranged by size: 2120 1304 1148 756 677 245 81 NaeI GCC'GGC Cuts at: 3374 3374 Size: 6331 NcoI C'CATG_G Cuts at: 513 1065 2004 3081 513 Size: 552 939 1077 3763 Fragments arranged by size: 3763 1077 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6331 NgoMIV G'CCGG_C Cuts at: 3372 3372 Size: 6331 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6331 NotI GC'GGCC_GC Cuts at: 3778 3778 Size: 6331 NspI r_CATG'y Cuts at: 1132 1229 2123 3689 5234 1132 Size: 97 894 1566 1545 2229 Fragments arranged by size: 2229 1566 1545 894 97 PasI CC'CwG_GG Cuts at: 1348 2432 2567 1348 Size: 1084 135 5112 Fragments arranged by size: 5112 1084 135 PciI A'CATG_T Cuts at: 5230 5230 Size: 6331 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6331 PmeI GTTT'AAAC Cuts at: 3635 3635 Size: 6331 PmlI CAC'GTG Cuts at: 2260 2260 Size: 6331 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4400 4432 5913 5945 4400 Size: 32 1481 32 4786 Fragments arranged by size: 4786 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3136 3352 3136 Size: 216 6115 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6331 PsiI TTA'TAA Cuts at: 2379 3901 2379 Size: 1522 4809 PspOMI G'GGCC_C Cuts at: 3086 3086 Size: 6331 PstI C_TGCA'G Cuts at: 830 3683 830 Size: 2853 3478 PvuI CG_AT'CG Cuts at: 2001 2333 2001 Size: 332 5999 PvuII CAG'CTG Cuts at: 2910 2991 2910 Size: 81 6250 SacI G_AGCT'C Cuts at: 2390 2390 Size: 6331 SalI G'TCGA_C Cuts at: 1936 3673 1936 Size: 1737 4594 SbfI CC_TGCA'GG Cuts at: 3683 3683 Size: 6331 ScaI AGT'ACT Cuts at: 4579 6291 4579 Size: 1712 4619 SexAI A'CCwGG_T Cuts at: 2371 2371 Size: 6331 SfcI C'TryA_G Cuts at: 826 1045 3050 3377 3679 4814 5495 5686 826 Size: 219 2005 327 302 1135 681 191 1471 Fragments arranged by size: 2005 1471 1135 681 327 302 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6331 SmaI CCC'GGG Cuts at: 1872 3658 1872 Size: 1786 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4435 5336 5598 820 Size: 197 926 2492 901 262 1553 Fragments arranged by size: 2492 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4122 493 Size: 3629 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6331 SphI G_CATG'C Cuts at: 1132 3689 1132 Size: 2557 3774 SspI AAT'ATT Cuts at: 5 52 2932 4255 5 Size: 47 2880 1323 2081 Fragments arranged by size: 2880 2081 1323 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6331 StyI C'CwwG_G Cuts at: 513 1065 2004 2684 3081 3417 3770 513 Size: 552 939 680 397 336 353 3074 Fragments arranged by size: 3074 939 680 552 397 353 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3205 4358 4375 4528 4713 5158 325 Size: 1255 1625 1153 17 153 185 445 1498 Fragments arranged by size: 1625 1498 1255 1153 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2122 3070 4577 Size: 274 80 33 51 1443 145 948 1507 Cuts at: 4577 6289 96 Size: 1712 138 Fragments arranged by size: 1712 1507 1443 948 274 145 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6291 6323 6291 Size: 32 6299 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6331 XbaI T'CTAG_A Cuts at: 3667 3667 Size: 6331 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2666 3167 2666 Size: 501 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6331 XmaI C'CCGG_G Cuts at: 1870 3656 1870 Size: 1786 4545 XmnI GAAnn'nnTTC Cuts at: 4460 4460 Size: 6331 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4139 276 Size: 53 83 186 936 2605 2468 Fragments arranged by size: 2605 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI