Restriction map for
pFN21AB9808
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BseRI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluNI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 184 CGCG CG^CG
AccIII 668 TCCGGA T^CCGGA
AcsI 394 GAATTC [GA]^AATT[CT]
AflIII 183 ACGCGT A^C[GA][CT]GT
AlwI 303 GGATC GGATC(4/5)
AlwNI 301 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 394 GAATTC [GA]^AATT[CT]
AspHI 694 GTGCTC G[AT]GC[AT]^C
AspI 409 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 563 GGTGCC G^G[CT][GA]CC
BanII 149 GAGCCC G[GA]GC[CT]^C
BclI 23 TGATCA T^GATCA
BlpI 696 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 696 GCTCAGC GC^T[ACGT]AGC
BsaI 526 GAGACC GGTCTC(1/5)
BsaWI 668 TCCGGA [AT]^CCGG[AT]
BseAI 668 TCCGGA T^CCGGA
BsgI 179 CTGCAC GTGCAG(16/14)
BsiHKAI 694 GTGCTC G[AT]GC[AT]^C
BsmAI 526 GAGAC GTCTC(1/5)
BsmI 123 GCATTC GAATGC(1/-1)
BspEI 668 TCCGGA T^CCGGA
BsrDI 71 GCAATG GCAATG(2/0)
BstUI 184 CGCG CG^CG
BstYI 303 GGATCT [GA]^GATC[CT]
CelII 696 GCTCAGC GC^T[ACGT]AGC
DraI 229 TTTAAA TTT^AAA
DraII 539 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 251 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 310 GACTTCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 635 CCATGG C^C[GA][CT]GG
EarI 716 GAAGAG CTCTTC(1/4)
EcoNI 335 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 539 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 394 GAATTC G^AATTC
EcoT14I 635 CCATGG C^C[AT][AT]GG
FbaI 23 TGATCA T^GATCA
HaeIII 681 GGCC GG^CC
HincII 553 GTTGAC GT[CT]^[GA]AC
HindII 553 GTTGAC GT[CT]^[GA]AC
Ksp632I 716 GAAGAG CTCTTC(1/4)
MaeIII 407 GTGAC ^GT[ACGT]AC
MflI 303 GGATCT [GA]^GATC[CT]
MluI 183 ACGCGT A^CGCGT
MroI 668 TCCGGA T^CCGGA
MslI 207 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 184 CGCG CG^CG
NcoI 635 CCATGG C^CATGG
PflFI 409 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 539 GGGACCC [GA]G^G[AT]CC[CT]
SapI 716 GAAGAGC GCTCTTC(1/4)
SbfI 79 CCTGCAGG CCTGCA^GG
ScaI 515 AGTACT AGT^ACT
Sse8387I 79 CCTGCAGG CCTGCA^GG
StyI 635 CCATGG C^C[AT][AT]GG
TaqI 314 TCGA T^CGA
Tsp45I 407 GTGAC ^GT[GC]AC
Tsp509I 395 AATT ^AATT
Tth111I 409 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 314 TCGA T^CGA
XhoII 303 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 516 GTAC GT^AC
602 GTAC
AvaII 216 GGACC G^G[AT]CC
540 GGACC
BmyI 149 GAGCCC G[AGT]GC[ACT]^C
694 GTGCTC
BsmFI 539 GGGAC GGGAC(10/14)
76 GTCCC
Bsp1286I 149 GAGCCC G[AGT]GC[ACT]^C
694 GTGCTC
BsrI 107 ACTGG ACTGG(1/-1)
587 ACTGG
BstNI 221 CCAGG CC^[AT]GG
656 CCTGG
CfoI 158 GCGC GCG^C
628 GCGC
Cfr13I 216 GGACC G^G[ACGT]CC
540 GGACC
EcoRII 221 CCAGG ^CC[AT]GG
656 CCTGG
HaeII 157 AGCGCC [GA]GCGC^[CT]
627 GGCGCT
HhaI 158 GCGC GCG^C
628 GCGC
HinP1I 158 GCGC G^CGC
628 GCGC
MboII 716 GAAGA GAAGA(8/7)
380 TCTTC
MseI 230 TTAA T^TAA
494 TTAA
MspA1I 167 CAGCGG C[AC]G^C[GT]G
558 CAGCGG
MvaI 221 CCAGG CC^[AT]GG
656 CCTGG
NlaIII 6 CATG CATG^
636 CATG
PstI 80 CTGCAG CTGCA^G
290 CTGCAG
RsaI 516 GTAC GT^AC
602 GTAC
Sau96I 216 GGACC G^G[ACGT]CC
540 GGACC
SfcI 80 CTGCAG C^T[GA][CT]AG
290 CTGCAG
Tru9I 230 TTAA T^TAA
494 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI BstF5I Cac8I
DdeI DpnII FauI Fnu4HI FokI
HapII HinfI HpaII HphI ItaI
MboI MnlI MspI MwoI NciI
NdeII NlaIV PleI Sau3AI ScrFI
TseI TspRI