Restriction map for
pFN21AB9808
(Linear) (Six-base) MAPSORT of: KIBB9808.seq Check: 924 from: 1 to: 723
KIBB9808.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 723
Size: 103 620
AflIII A'CryG_T
Cuts at: 0 183 723
Size: 183 540
AlwNI CAG_nnn'CTG
Cuts at: 0 306 723
Size: 306 417
ApoI r'AATT_y
Cuts at: 0 394 723
Size: 394 329
BanI G'GyrC_C
Cuts at: 0 563 723
Size: 563 160
BanII G_rGCy'C
Cuts at: 0 153 723
Size: 153 570
BclI T'GATC_A
Cuts at: 0 23 723
Size: 23 700
BlpI GC'TnA_GC
Cuts at: 0 697 723
Size: 697 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 723
Size: 520 203
BsaWI w'CCGG_w
Cuts at: 0 668 723
Size: 668 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 261 291 723
Size: 261 30 432
Fragments arranged by size:
432 261 30
BseYI C'CCAG_C
Cuts at: 0 272 723
Size: 272 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 723
Size: 164 559
BsiHKAI G_wGCw'C
Cuts at: 0 698 723
Size: 698 25
BsmI GAATG_Cn'
Cuts at: 0 123 723
Size: 123 600
Bsp1286I G_dGCh'C
Cuts at: 0 153 698 723
Size: 153 545 25
Fragments arranged by size:
545 153 25
BspEI T'CCGG_A
Cuts at: 0 668 723
Size: 668 55
BsrDI GCAATG_nn'
Cuts at: 0 78 723
Size: 78 645
BstYI r'GATC_y
Cuts at: 0 303 723
Size: 303 420
BtgI C'CryG_G
Cuts at: 0 635 723
Size: 635 88
BtsI GCAGTG_nn'
Cuts at: 0 134 723
Size: 134 589
DraI TTT'AAA
Cuts at: 0 231 723
Size: 231 492
DraIII CAC_nnn'GTG
Cuts at: 0 256 723
Size: 256 467
DrdI GACnn_nn'nnGTC
Cuts at: 0 316 723
Size: 316 407
EarI CTCTTCn'nnn_
Cuts at: 0 711 723
Size: 711 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 723
Size: 67 656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 723
Size: 103 620
EcoNI CCTnn'n_nnAGG
Cuts at: 0 339 723
Size: 339 384
EcoO109I rG'GnC_Cy
Cuts at: 0 540 723
Size: 540 183
EcoRI G'AATT_C
Cuts at: 0 394 723
Size: 394 329
HaeII r_GCGC'y
Cuts at: 0 161 631 723
Size: 161 470 92
Fragments arranged by size:
470 161 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 77 109 723
Size: 77 32 614
Fragments arranged by size:
614 77 32
HincII GTy'rAC
Cuts at: 0 555 723
Size: 555 168
MluI A'CGCG_T
Cuts at: 0 183 723
Size: 183 540
MslI CAynn'nnrTG
Cuts at: 0 211 723
Size: 211 512
MspA1I CmG'CkG
Cuts at: 0 169 560 723
Size: 169 391 163
Fragments arranged by size:
391 169 163
NcoI C'CATG_G
Cuts at: 0 635 723
Size: 635 88
PpuMI rG'GwC_Cy
Cuts at: 0 540 723
Size: 540 183
PstI C_TGCA'G
Cuts at: 0 84 294 723
Size: 84 210 429
Fragments arranged by size:
429 210 84
SanDI GG'GwC_CC
Cuts at: 0 540 723
Size: 540 183
SapI GCTCTTCn'nnn_
Cuts at: 0 711 723
Size: 711 12
SbfI CC_TGCA'GG
Cuts at: 0 84 723
Size: 84 639
ScaI AGT'ACT
Cuts at: 0 517 723
Size: 517 206
SfcI C'TryA_G
Cuts at: 0 80 290 723
Size: 80 210 433
Fragments arranged by size:
433 210 80
StyI C'CwwG_G
Cuts at: 0 635 723
Size: 635 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 286 723
Size: 286 437
TatI w'GTAC_w
Cuts at: 0 515 723
Size: 515 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 261 293 723
Size: 261 32 430
Fragments arranged by size:
430 261 32
Tth111I GACn'n_nGTC
Cuts at: 0 412 723
Size: 412 311
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BanI BanII BclI BlpI
BsaI BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BsrDI BstYI BtgI BtsI DraI DraIII DrdI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII MluI MslI MspA1I NcoI PpuMI PstI SanDI
SapI SbfI ScaI SfcI StyI TaqII TatI TstI
Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BseRI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI EaeI EagI EcoICRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XcmI XhoI
XmaI XmnI ZraI