(Circular) (Six-base) MAPSORT of: pFN21AB6290.seq Check: 8348 from: 1 to: 7060 pFN21AB6290 7060 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3734 3827 3939 4306 3734 Size: 93 112 367 6488 Fragments arranged by size: 6488 367 112 93 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4870 278 Size: 53 83 186 936 3334 2468 Fragments arranged by size: 3334 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4403 1937 Size: 2466 4594 Acc65I G'GTAC_C Cuts at: 4381 4381 Size: 7060 AclI AA'CG_TT Cuts at: 1563 5187 5560 1563 Size: 3624 373 3063 Fragments arranged by size: 3624 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3308 3606 4209 5123 6507 6775 1632 Size: 1676 298 603 914 1384 268 1917 Fragments arranged by size: 1917 1676 1384 914 603 298 268 AfeI AGC'GCT Cuts at: 4085 4085 Size: 7060 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6863 AflIII A'CryG_T Cuts at: 1203 4007 5959 6936 1203 Size: 2804 1952 977 1327 Fragments arranged by size: 2804 1952 1327 977 AgeI A'CCGG_T Cuts at: 5891 5891 Size: 7060 AhdI GACnn_n'nnGTC Cuts at: 4105 5789 4105 Size: 1684 5376 AleI CACnn'nnGTG Cuts at: 1166 4047 1166 Size: 2881 4179 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2221 2589 3835 1184 Size: 1037 368 1246 4409 Fragments arranged by size: 4409 1246 1037 368 AlwNI CAG_nnn'CTG Cuts at: 2432 3554 4803 6375 2432 Size: 1122 1249 1572 3117 Fragments arranged by size: 3117 1572 1249 1122 ApaI G_GGCC'C Cuts at: 2255 2587 3378 2255 Size: 332 791 5937 Fragments arranged by size: 5937 791 332 ApaLI G'TGCA_C Cuts at: 3635 5117 6273 3635 Size: 1482 1156 4422 Fragments arranged by size: 4422 1482 1156 ApoI r'AATT_y Cuts at: 1493 3198 4058 4369 4595 4747 6856 1493 Size: 1705 860 311 226 152 2109 1697 Fragments arranged by size: 2109 1705 1697 860 311 226 152 AseI AT'TA_AT Cuts at: 160 1032 4338 5614 160 Size: 872 3306 1276 1606 Fragments arranged by size: 3306 1606 1276 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 AvaI C'yCGr_G Cuts at: 1870 1943 2254 3853 4385 1870 Size: 73 311 1599 532 4545 Fragments arranged by size: 4545 1599 532 311 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2754 2787 2754 Size: 33 7027 BamHI G'GATC_C Cuts at: 3859 4390 4757 3859 Size: 531 367 6162 Fragments arranged by size: 6162 531 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2312 4381 5836 618 Size: 325 217 627 315 210 2069 1455 1842 Fragments arranged by size: 2069 1842 1455 627 325 315 217 210 BanII G_rGCy'C Cuts at: 1391 2255 2587 3378 3694 3854 4379 1391 Size: 864 332 791 316 160 525 4072 Fragments arranged by size: 4072 864 791 525 332 316 160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2403 928 Size: 971 504 5585 Fragments arranged by size: 5585 971 504 BbvCI CC'TCA_GC Cuts at: 3213 3523 3213 Size: 310 6750 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 5251 5285 1903 Size: 34 2402 34 878 34 3678 Fragments arranged by size: 3678 2402 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4952 6162 4952 Size: 1210 5850 BclI T'GATC_A Cuts at: 1548 3682 1548 Size: 2134 4926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2582 3242 4259 Size: 107 122 71 631 605 910 660 1017 Cuts at: 4259 5671 136 Size: 1412 1525 Fragments arranged by size: 1525 1412 1017 910 660 631 605 122 107 71 BglII A'GATC_T Cuts at: 4324 7055 4324 Size: 2731 4329 BlpI GC'TnA_GC Cuts at: 3693 4477 3693 Size: 784 6276 Bme1580I G_kGCm'C Cuts at: 1790 2255 2587 3378 3639 3940 4172 5121 6277 Size: 465 332 791 261 301 232 949 1156 Cuts at: 6277 1790 Size: 2573 Fragments arranged by size: 2573 1156 949 791 465 332 301 261 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7060 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2589 4791 5749 Size: 416 53 83 399 24 1172 2202 958 Cuts at: 5749 442 Size: 1753 Fragments arranged by size: 2202 1753 1172 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7060 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 7060 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 2520 2684 3153 3224 3293 3302 3575 Size: 933 348 164 469 71 69 9 273 Cuts at: 3575 5720 1239 Size: 2145 2579 Fragments arranged by size: 2579 2145 933 469 348 273 164 71 69 9 Bpu10I CC'TnA_GC Cuts at: 3213 3454 3523 4080 3213 Size: 241 69 557 6193 Fragments arranged by size: 6193 557 241 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3042 3270 5185 6050 6348 3042 Size: 228 1915 865 298 3754 Fragments arranged by size: 3754 1915 865 298 228 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3707 5723 882 Size: 617 2208 2016 2219 Fragments arranged by size: 2219 2208 2016 617 BsaAI yAC'GTr Cuts at: 493 1204 4851 493 Size: 711 3647 2702 Fragments arranged by size: 3647 2702 711 BsaBI GATnn'nnATC Cuts at: 7054 7054 Size: 7060 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4867 5249 5884 275 Size: 53 83 186 936 3334 382 635 1451 Fragments arranged by size: 3334 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3357 5493 5891 6165 6312 6723 3357 Size: 2136 398 274 147 411 3694 Fragments arranged by size: 3694 2136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2821 2851 3222 3252 4204 Size: 30 165 30 1418 30 371 30 952 Cuts at: 4204 4234 1178 Size: 30 4004 Fragments arranged by size: 4004 1418 952 371 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2169 2172 2332 3565 4108 1186 Size: 983 3 160 1233 543 4138 Fragments arranged by size: 4138 1233 983 543 160 3 BseYI C'CCAG_C Cuts at: 2172 2840 6263 6759 2172 Size: 668 3423 496 2473 Fragments arranged by size: 3423 2473 668 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2612 1163 Size: 573 876 5611 Fragments arranged by size: 5611 876 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3793 4510 5271 6299 1540 Size: 275 186 1792 717 761 1028 2301 Fragments arranged by size: 2301 1792 1028 761 717 275 186 BsiHKAI G_wGCw'C Cuts at: 3639 3694 5121 5206 6277 3639 Size: 55 1427 85 1071 4422 Fragments arranged by size: 4422 1427 1071 85 55 BsmI GAATG_Cn' Cuts at: 3565 4571 4664 3565 Size: 1006 93 5961 Fragments arranged by size: 5961 1006 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2255 2587 3378 3639 3694 3854 3940 Size: 399 465 332 791 261 55 160 86 Cuts at: 3940 4172 4379 5121 5206 6277 1391 Size: 232 207 742 85 1071 2174 Fragments arranged by size: 2174 1071 791 742 465 399 332 261 232 207 160 86 85 55 BspEI T'CCGG_A Cuts at: 3357 6723 3357 Size: 3366 3694 BspHI T'CATG_A Cuts at: 4089 4949 6679 4089 Size: 860 1730 4470 Fragments arranged by size: 4470 1730 860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3734 3827 3939 4184 4306 4415 844 Size: 2890 93 112 245 122 109 3489 Fragments arranged by size: 3489 2890 245 122 112 109 93 BsrBI CCG'CTC Cuts at: 1394 1823 2459 4947 1394 Size: 429 636 2488 3507 Fragments arranged by size: 3507 2488 636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2092 2404 2676 3140 3516 4278 4357 Size: 1183 844 312 272 464 376 762 79 Cuts at: 4357 5555 5729 65 Size: 1198 174 1396 Fragments arranged by size: 1396 1198 1183 844 762 464 376 312 272 174 79 BsrFI r'CCGG_y Cuts at: 1700 1810 3788 5704 5891 1700 Size: 110 1978 1916 187 2869 Fragments arranged by size: 2869 1978 1916 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7060 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7060 BssSI C'ACGA_G Cuts at: 5120 6132 5120 Size: 1012 6048 BstAPI GCAn_nnn'nTGC Cuts at: 794 2563 794 Size: 1769 5291 BstBI TT'CG_AA Cuts at: 5864 5864 Size: 7060 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7060 BstXI CCAn_nnnn'nTGG Cuts at: 2885 3010 3131 3713 3902 4246 2885 Size: 125 121 582 189 344 5699 Fragments arranged by size: 5699 582 344 189 125 121 BstYI r'GATC_y Cuts at: 1972 3859 4324 4390 4757 5142 5159 6611 6697 Size: 1887 465 66 367 385 17 1452 86 Cuts at: 6697 6709 7055 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1887 1452 465 385 367 346 86 66 17 12 Bsu36I CC'TnA_GG Cuts at: 2905 2905 Size: 7060 BtgI C'CryG_G Cuts at: 513 1065 2025 3706 4254 513 Size: 552 960 1681 548 3319 Fragments arranged by size: 3319 1681 960 552 548 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6321 Fragments arranged by size: 6321 683 56 BtsI GCAGTG_nn' Cuts at: 2410 4212 4213 4576 5370 5390 2410 Size: 1802 1 363 794 20 4080 Fragments arranged by size: 4080 1802 794 363 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7060 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7025 DraI TTT'AAA Cuts at: 4364 4364 Size: 7060 DraIII CAC_nnn'GTG Cuts at: 2312 2312 Size: 7060 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6067 809 Size: 507 4751 1802 Fragments arranged by size: 4751 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4507 5396 7025 Size: 54 1425 50 275 18 2677 889 1629 Cuts at: 7025 8 Size: 43 Fragments arranged by size: 2677 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4507 1537 Size: 275 2695 4090 Fragments arranged by size: 4090 2695 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2341 4990 1353 Size: 988 2649 3423 Fragments arranged by size: 3423 2649 988 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5647 6021 6167 387 Size: 1064 138 4058 374 146 1280 Fragments arranged by size: 4058 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3692 3692 Size: 7060 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2520 2684 3153 3224 3293 3302 Size: 393 540 348 164 469 71 69 9 Cuts at: 3302 3308 3575 3606 4209 5123 5720 6507 6775 Size: 6 267 31 603 914 597 787 268 Cuts at: 6775 1239 Size: 1524 Fragments arranged by size: 1524 914 787 603 597 540 469 393 348 268 267 164 71 69 31 9 6 EcoO109I rG'GnC_Cy Cuts at: 2251 2583 2874 3235 3244 3374 3798 2251 Size: 332 291 361 9 130 424 5513 Fragments arranged by size: 5513 424 361 332 291 130 9 EcoRI G'AATT_C Cuts at: 4369 4747 4369 Size: 378 6682 FspI TGC'GCA Cuts at: 5566 5566 Size: 7060 HaeII r_GCGC'y Cuts at: 1678 4087 6207 1678 Size: 2409 2120 2531 Fragments arranged by size: 2531 2409 2120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2658 2690 3040 3072 3222 Size: 32 135 32 1086 32 350 32 150 Cuts at: 3222 3254 4091 4123 4316 4348 5701 5733 5775 Size: 32 837 32 193 32 1353 32 42 Cuts at: 5775 5807 1373 Size: 32 2626 Fragments arranged by size: 2626 1353 1086 837 350 193 150 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2812 4404 4650 669 Size: 988 281 874 1592 246 3079 Fragments arranged by size: 3079 1592 988 874 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7060 HpaI GTT'AAC Cuts at: 4650 4650 Size: 7060 KpnI G_GTAC'C Cuts at: 4385 4385 Size: 7060 MfeI C'AATT_G Cuts at: 4659 4659 Size: 7060 MluI A'CGCG_T Cuts at: 6936 6936 Size: 7060 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3773 6174 6358 1305 Size: 2468 2401 184 2007 Fragments arranged by size: 2468 2401 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5238 Fragments arranged by size: 5238 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3973 4047 4244 4316 5018 5377 5536 Size: 648 2807 74 197 72 702 359 159 Cuts at: 5536 518 Size: 2042 Fragments arranged by size: 2807 2042 702 648 359 197 159 74 72 MspA1I CmG'CkG Cuts at: 1606 2042 2563 3409 3527 4476 5153 6301 6546 Size: 436 521 846 118 949 677 1148 245 Cuts at: 6546 1606 Size: 2120 Fragments arranged by size: 2120 1148 949 846 677 521 436 245 118 NcoI C'CATG_G Cuts at: 513 1065 2025 3706 4254 513 Size: 552 960 1681 548 3319 Fragments arranged by size: 3319 1681 960 552 548 NdeI CA'TA_TG Cuts at: 387 387 Size: 7060 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7060 NotI GC'GGCC_GC Cuts at: 4507 4507 Size: 7060 NspI r_CATG'y Cuts at: 1132 1229 3816 4418 5963 1132 Size: 97 2587 602 1545 2229 Fragments arranged by size: 2587 2229 1545 602 97 PasI CC'CwG_GG Cuts at: 1348 2248 1348 Size: 900 6160 PciI A'CATG_T Cuts at: 5959 5959 Size: 7060 PflMI CCAn_nnn'nTGG Cuts at: 1108 2322 2725 3341 1108 Size: 1214 403 616 4827 Fragments arranged by size: 4827 1214 616 403 PmeI GTTT'AAAC Cuts at: 4364 4364 Size: 7060 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5129 5161 6642 6674 5129 Size: 32 1481 32 5515 Fragments arranged by size: 5515 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2874 3798 2874 Size: 924 6136 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7060 PsiI TTA'TAA Cuts at: 4630 4630 Size: 7060 PspOMI G'GGCC_C Cuts at: 2251 2583 3374 2251 Size: 332 791 5937 Fragments arranged by size: 5937 791 332 PstI C_TGCA'G Cuts at: 830 2568 2827 3414 4332 4412 830 Size: 1738 259 587 918 80 3478 Fragments arranged by size: 3478 1738 918 587 259 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7060 PvuII CAG'CTG Cuts at: 2042 2563 3409 3527 2042 Size: 521 846 118 5575 Fragments arranged by size: 5575 846 521 118 SacI G_AGCT'C Cuts at: 3694 3694 Size: 7060 SalI G'TCGA_C Cuts at: 1936 4402 1936 Size: 2466 4594 SanDI GG'GwC_CC Cuts at: 2874 2874 Size: 7060 SbfI CC_TGCA'GG Cuts at: 4412 4412 Size: 7060 ScaI AGT'ACT Cuts at: 5308 7020 5308 Size: 1712 5348 SexAI A'CCwGG_T Cuts at: 2626 3980 2626 Size: 1354 5706 SfcI C'TryA_G Cuts at: 826 1045 2564 2823 3089 3404 3410 3663 4022 Size: 219 1519 259 266 315 6 253 359 Cuts at: 4022 4328 4408 5543 6224 6415 826 Size: 306 80 1135 681 191 1471 Fragments arranged by size: 1519 1471 1135 681 359 315 306 266 259 253 219 191 80 6 SfiI GGCCn_nnn'nGGCC Cuts at: 3242 3242 Size: 7060 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7060 SmaI CCC'GGG Cuts at: 1872 4387 1872 Size: 2515 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3057 3285 5164 6065 6327 820 Size: 197 926 1114 228 1879 901 262 1553 Fragments arranged by size: 1879 1553 1114 926 901 262 228 197 SnaBI TAC'GTA Cuts at: 493 4851 493 Size: 4358 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7060 SphI G_CATG'C Cuts at: 1132 3816 4418 1132 Size: 2684 602 3774 Fragments arranged by size: 3774 2684 602 SspI AAT'ATT Cuts at: 5 52 2515 4984 5 Size: 47 2463 2469 2081 Fragments arranged by size: 2469 2463 2081 47 StuI AGG'CCT Cuts at: 1517 2724 3588 3651 1517 Size: 1207 864 63 4926 Fragments arranged by size: 4926 1207 864 63 StyI C'CwwG_G Cuts at: 513 1065 2025 2719 3477 3706 3895 4254 4499 Size: 552 960 694 758 229 189 359 245 Cuts at: 4499 513 Size: 3074 Fragments arranged by size: 3074 960 758 694 552 359 245 229 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2239 3529 3654 5087 5104 5257 5442 Size: 1255 659 1290 125 1433 17 153 185 Cuts at: 5442 5887 325 Size: 445 1498 Fragments arranged by size: 1498 1433 1290 1255 659 445 185 153 125 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2737 5306 7018 Size: 274 80 33 51 1443 760 2569 1712 Cuts at: 7018 96 Size: 138 Fragments arranged by size: 2569 1712 1443 760 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3618 3650 4204 4236 7020 7052 3618 Size: 32 554 32 2784 32 3626 Fragments arranged by size: 3626 2784 554 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7060 XbaI T'CTAG_A Cuts at: 4396 4396 Size: 7060 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2549 2773 3007 3080 3713 2549 Size: 224 234 73 633 5896 Fragments arranged by size: 5896 633 234 224 73 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7060 XmaI C'CCGG_G Cuts at: 1870 4385 1870 Size: 2515 4545 XmnI GAAnn'nnTTC Cuts at: 4001 5189 4001 Size: 1188 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4868 276 Size: 53 83 186 936 3334 2468 Fragments arranged by size: 3334 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfoI SrfI SwaI