Restriction map for
pFN21AB0470
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# NO CUT ENZYMES ( 92 enzymes) #
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AatII Acc65I AccII AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BbeI BcgI BfrI BglII
BlnI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoNI EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tsp509I TthHB8I
XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 2227 GTAGAC GT^[AC][GT]AC
AccIII 1796 TCCGGA T^CCGGA
AfaI 2436 GTAC GT^AC
Aor51HI 236 AGCGCT AGC^GCT
Asp700I 2091 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 566 TGCGCA TGC^GCA
BbrPI 1870 CACGTG CAC^GTG
BciVI 2578 GGATAC GTATCC(6/5)
BclI 1277 TGATCA T^GATCA
BsaAI 1870 CACGTG [CT]AC^GT[GA]
BsaBI 2463 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1796 TCCGGA [AT]^CCGG[AT]
BseAI 1796 TCCGGA T^CCGGA
BsiEI 1752 CGGCCG CG[GA][CT]^CG
BsmBI 478 GAGACG CGTCTC(1/5)
BsmI 449 GCATTC GAATGC(1/-1)
BspEI 1796 TCCGGA T^CCGGA
BsrDI 170 CATTGC GCAATG(2/0)
BssSI 2078 CTCGTG CACGAG(-5/-1)
BstEII 2530 GGTCACC G^GT[ACGT]ACC
BstPI 2530 GGTCACC G^GT[ACGT]ACC
DraIII 2543 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1752 CGGCCG C^GGCCG
EclXI 1752 CGGCCG C^GGCCG
Eco47III 236 AGCGCT AGC^GCT
Eco52I 1752 CGGCCG C^GGCCG
EcoO65I 2530 GGTCACC G^GT[ACGT]ACC
FbaI 1277 TGATCA T^GATCA
FspI 566 TGCGCA TGC^GCA
MamI 2463 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1796 TCCGGA T^CCGGA
MseI 1129 TTAA T^TAA
NheI 381 GCTAGC G^CTAGC
NspI 2807 GCATGC [GA]CATG^[CT]
PmaCI 1870 CACGTG CAC^GTG
PmlI 1870 CACGTG CAC^GTG
RsaI 2436 GTAC GT^AC
SphI 2807 GCATGC GCATG^C
Tru9I 1129 TTAA T^TAA
XmnI 2091 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 2665 GACGCC G[GA]^CG[CT]C
2895 GGCGTC
Alw44I 2239 GTGCAC G^TGCAC
2785 GTGCAC
ApaLI 2239 GTGCAC G^TGCAC
2785 GTGCAC
BamHI 2427 GGATCC G^GATCC
2709 GGATCC
BbsI 1336 GAAGAC GAAGAC(2/6)
1572 GTCTTC
BbvCI 103 CCTCAGC CCTCAGC(-5/-2)
2524 GCTGAGG
BlpI 504 GCTCAGC GC^T[ACGT]AGC
1896 GCTCAGC
Bpu1102I 504 GCTCAGC GC^T[ACGT]AGC
1896 GCTCAGC
BpuAI 1336 GAAGAC GAAGAC(2/6)
1572 GTCTTC
BsaHI 2665 GACGCC G[GA]^CG[CT]C
2895 GGCGTC
BsaI 691 GAGACC GGTCTC(1/5)
1645 GAGACC
BsgI 199 CTGCAC GTGCAG(16/14)
86 GTGCAG
CelII 504 GCTCAGC GC^T[ACGT]AGC
1896 GCTCAGC
Hin1I 2665 GACGCC G[GA]^CG[CT]C
2895 GGCGTC
NaeI 1750 GCCGGC GCC^GGC
2183 GCCGGC
NgoMIV 1750 GCCGGC G^CCGGC
2183 GCCGGC
PvuII 2188 CAGCTG CAG^CTG
2941 CAGCTG
SapI 1840 GAAGAGC GCTCTTC(1/4)
2689 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
ApaI AspHI AspI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TfiI TseI
Tsp45I TspRI Tth111I Van91I XcmI
XhoII XmaCI XmaI