(Circular) (Six-base) MAPSORT of: pFN21AB0470.seq Check: 2945 from: 1 to: 7678 pFN21AB0470 7678 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2284 3601 2284 Size: 1317 6361 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5488 278 Size: 53 83 186 936 3952 2468 Fragments arranged by size: 3952 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4233 5021 1937 Size: 2296 788 4594 Fragments arranged by size: 4594 2296 788 Acc65I G'GTAC_C Cuts at: 4999 4999 Size: 7678 AclI AA'CG_TT Cuts at: 1563 5805 6178 1563 Size: 4242 373 3063 Fragments arranged by size: 4242 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2618 3360 3728 4947 5741 7125 7393 1632 Size: 986 742 368 1219 794 1384 268 1917 Fragments arranged by size: 1917 1384 1219 986 794 742 368 268 AfeI AGC'GCT Cuts at: 2243 2243 Size: 7678 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7481 AflIII A'CryG_T Cuts at: 1203 6577 7554 1203 Size: 5374 977 1327 Fragments arranged by size: 5374 1327 977 AgeI A'CCGG_T Cuts at: 6509 6509 Size: 7678 AhdI GACnn_n'nnGTC Cuts at: 6407 6407 Size: 7678 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7678 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2537 2761 1184 Size: 1353 224 6101 Fragments arranged by size: 6101 1353 224 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2422 2454 2422 Size: 32 7646 AlwNI CAG_nnn'CTG Cuts at: 2088 2203 2585 2818 3487 3617 4600 4822 4865 Size: 115 382 233 669 130 983 222 43 Cuts at: 4865 5421 6993 2088 Size: 556 1572 2773 Fragments arranged by size: 2773 1572 983 669 556 382 233 222 130 115 43 ApaI G_GGCC'C Cuts at: 2948 3108 3239 2948 Size: 160 131 7387 Fragments arranged by size: 7387 160 131 ApaLI G'TGCA_C Cuts at: 4244 4790 5735 6891 4244 Size: 546 945 1156 5031 Fragments arranged by size: 5031 1156 945 546 ApoI r'AATT_y Cuts at: 1493 4987 5213 5365 7474 1493 Size: 3494 226 152 2109 1697 Fragments arranged by size: 3494 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6232 160 Size: 872 5200 1606 Fragments arranged by size: 5200 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7678 AvaI C'yCGr_G Cuts at: 1870 1943 2041 2285 2949 3008 3095 3504 3567 Size: 73 98 244 664 59 87 409 63 Cuts at: 3567 3630 4336 4479 5003 1870 Size: 63 706 143 524 4545 Fragments arranged by size: 4545 706 664 524 409 244 143 98 87 73 63 63 59 BamHI G'GATC_C Cuts at: 4432 4714 5008 5375 4432 Size: 282 294 367 6735 Fragments arranged by size: 6735 367 294 282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 3020 3552 3741 3890 Size: 325 217 627 258 975 532 189 149 Cuts at: 3890 4259 4846 4999 6454 618 Size: 369 587 153 1455 1842 Fragments arranged by size: 1842 1455 975 627 587 532 369 325 258 217 189 153 149 BanII G_rGCy'C Cuts at: 1391 2042 2160 2172 2256 2478 2592 2795 2948 Size: 651 118 12 84 222 114 203 153 Cuts at: 2948 2972 3009 3108 3201 3239 3574 3801 3942 Size: 24 37 99 93 38 335 227 141 Cuts at: 3942 4997 1391 Size: 1055 4072 Fragments arranged by size: 4072 1055 651 335 227 222 203 153 141 118 114 99 93 84 38 37 24 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3348 3570 928 Size: 971 1449 222 5036 Fragments arranged by size: 5036 1449 971 222 BbvCI CC'TCA_GC Cuts at: 2109 4530 2109 Size: 2421 5257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5869 5903 1903 Size: 34 50 34 3848 34 3678 Fragments arranged by size: 3848 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4577 5570 6780 4577 Size: 993 1210 5475 Fragments arranged by size: 5475 1210 993 BclI T'GATC_A Cuts at: 1548 3282 1548 Size: 1734 5944 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2067 2260 3626 Size: 107 122 71 631 605 395 193 1366 Cuts at: 3626 4040 4679 4688 6289 136 Size: 414 639 9 1601 1525 Fragments arranged by size: 1601 1525 1366 639 631 605 414 395 193 122 107 71 9 BglII A'GATC_T Cuts at: 7673 7673 Size: 7678 BlpI GC'TnA_GC Cuts at: 2510 3902 5095 2510 Size: 1392 1193 5093 Fragments arranged by size: 5093 1392 1193 Bme1580I G_kGCm'C Cuts at: 1790 2050 2948 3023 3096 3108 3239 3555 4248 Size: 260 898 75 73 12 131 316 693 Cuts at: 4248 4794 5739 6895 1790 Size: 546 945 1156 2573 Fragments arranged by size: 2573 1156 945 898 693 546 316 260 131 75 73 12 BmgBI CAC'GTC Cuts at: 1318 3274 4243 1318 Size: 1956 969 4753 Fragments arranged by size: 4753 1956 969 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2144 2538 3121 Size: 416 53 83 399 24 727 394 583 Cuts at: 3121 3204 4246 4281 4559 4813 5409 6367 442 Size: 83 1042 35 278 254 596 958 1753 Fragments arranged by size: 1753 1042 958 727 596 583 416 399 394 278 254 83 83 53 35 24 BmtI G_CTAG'C Cuts at: 1056 2390 1056 Size: 1334 6344 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3169 3204 3169 Size: 35 7643 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2501 2648 3275 3368 3413 3431 3625 Size: 1253 9 147 627 93 45 18 194 Cuts at: 3625 4428 4476 4793 6338 1239 Size: 803 48 317 1545 2579 Fragments arranged by size: 2579 1545 1253 803 627 317 194 147 93 48 45 18 9 Bpu10I CC'TnA_GC Cuts at: 2109 4530 2109 Size: 2421 5257 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5803 6668 6966 5803 Size: 865 298 6515 Fragments arranged by size: 6515 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2690 3644 6341 882 Size: 617 1191 954 2697 2219 Fragments arranged by size: 2697 2219 1191 954 617 BsaAI yAC'GTr Cuts at: 493 1204 3877 5469 493 Size: 711 2673 1592 2702 Fragments arranged by size: 2702 2673 1592 711 BsaBI GATnn'nnATC Cuts at: 4472 7672 4472 Size: 3200 4478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4671 4901 5485 5867 Size: 53 83 186 936 3138 230 584 382 Cuts at: 5867 6502 275 Size: 635 1451 Fragments arranged by size: 3138 1451 936 635 584 382 230 186 83 53 BsaWI w'CCGG_w Cuts at: 3801 6111 6509 6783 6930 7341 3801 Size: 2310 398 274 147 411 4138 Fragments arranged by size: 4138 2310 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3704 3734 3796 3826 4608 Size: 30 165 30 2301 30 62 30 782 Cuts at: 4608 4638 1178 Size: 30 4218 Fragments arranged by size: 4218 2301 782 165 62 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2701 3041 3432 3872 4970 1186 Size: 1515 340 391 440 1098 3894 Fragments arranged by size: 3894 1515 1098 440 391 340 BseYI C'CCAG_C Cuts at: 2196 2534 2672 3014 3406 3491 3583 4038 4263 Size: 338 138 342 392 85 92 455 225 Cuts at: 4263 6881 7377 2196 Size: 2618 496 2497 Fragments arranged by size: 2618 2497 496 455 392 342 338 225 138 92 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2112 2189 1163 Size: 573 376 77 6652 Fragments arranged by size: 6652 573 376 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3760 5128 5889 6917 1540 Size: 275 186 1759 1368 761 1028 2301 Fragments arranged by size: 2301 1759 1368 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2562 2772 3399 3722 3753 3882 4248 4296 4794 Size: 210 627 323 31 129 366 48 498 Cuts at: 4794 5739 5824 6895 2562 Size: 945 85 1071 3345 Fragments arranged by size: 3345 1071 945 627 498 366 323 210 129 85 48 31 BsmI GAATG_Cn' Cuts at: 2454 5189 5282 2454 Size: 2735 93 4850 Fragments arranged by size: 4850 2735 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2477 2477 Size: 7678 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2050 2160 2172 2256 2478 2562 Size: 399 252 8 110 12 84 222 84 Cuts at: 2562 2592 2772 2795 2948 2972 3009 3023 3096 Size: 30 180 23 153 24 37 14 73 Cuts at: 3096 3108 3201 3239 3399 3555 3574 3722 3753 Size: 12 93 38 160 156 19 148 31 Cuts at: 3753 3801 3882 3942 4248 4296 4794 4997 5739 Size: 48 81 60 306 48 498 203 742 Cuts at: 5739 5824 6895 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 306 252 222 203 180 160 156 153 148 110 93 85 84 84 81 73 60 48 48 38 37 31 30 24 23 19 14 12 12 8 BspEI T'CCGG_A Cuts at: 3801 7341 3801 Size: 3540 4138 BspHI T'CATG_A Cuts at: 5567 7297 5567 Size: 1730 5948 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2284 3601 5033 844 Size: 1292 148 1317 1432 3489 Fragments arranged by size: 3489 1432 1317 1292 148 BsrBI CCG'CTC Cuts at: 1394 1823 2502 2632 3148 3221 3440 3793 3871 Size: 429 679 130 516 73 219 353 78 Cuts at: 3871 3901 4767 5565 1394 Size: 30 866 798 3507 Fragments arranged by size: 3507 866 798 679 516 429 353 219 130 78 73 30 BsrDI GCAATG_nn' Cuts at: 65 1248 2174 6173 6347 65 Size: 1183 926 3999 174 1396 Fragments arranged by size: 3999 1396 1183 926 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3555 3755 4188 6322 6509 1700 Size: 110 1745 200 433 2134 187 2869 Fragments arranged by size: 2869 2134 1745 433 200 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7678 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7678 BssSI C'ACGA_G Cuts at: 4083 5738 6750 4083 Size: 1655 1012 5011 Fragments arranged by size: 5011 1655 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7678 BstBI TT'CG_AA Cuts at: 6482 6482 Size: 7678 BstEII G'GTnAC_C Cuts at: 1184 4535 1184 Size: 3351 4327 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2371 4842 2179 Size: 192 2471 5015 Fragments arranged by size: 5015 2471 192 BstYI r'GATC_y Cuts at: 1972 4221 4432 4714 5008 5375 5760 5777 7229 Size: 2249 211 282 294 367 385 17 1452 Cuts at: 7229 7315 7327 7673 1972 Size: 86 12 346 1977 Fragments arranged by size: 2249 1977 1452 385 367 346 294 282 211 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3064 3423 3459 3064 Size: 359 36 7283 Fragments arranged by size: 7283 359 36 BtgI C'CryG_G Cuts at: 513 1065 2121 2268 2995 3412 4149 4249 4425 Size: 552 1056 147 727 417 737 100 176 Cuts at: 4425 513 Size: 3766 Fragments arranged by size: 3766 1056 737 727 552 417 176 147 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4165 4295 4459 487 Size: 683 56 2939 130 164 3706 Fragments arranged by size: 3706 2939 683 164 130 56 BtsI GCAGTG_nn' Cuts at: 2645 4057 4629 4893 5194 5988 6008 2645 Size: 1412 572 264 301 794 20 4315 Fragments arranged by size: 4315 1412 794 572 301 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7678 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7643 DraI TTT'AAA Cuts at: 4982 4982 Size: 7678 DraIII CAC_nnn'GTG Cuts at: 4553 4553 Size: 7678 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6685 809 Size: 507 5369 1802 Fragments arranged by size: 5369 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3173 3757 4176 Size: 54 1425 50 275 18 1343 584 419 Cuts at: 4176 4645 5125 6014 7643 8 Size: 469 480 889 1629 43 Fragments arranged by size: 1629 1425 1343 889 584 480 469 419 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3757 5125 1537 Size: 275 1945 1368 4090 Fragments arranged by size: 4090 1945 1368 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2990 3840 4701 5608 1353 Size: 869 768 850 861 907 3423 Fragments arranged by size: 3423 907 869 861 850 768 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6265 6639 6785 387 Size: 1064 138 4676 374 146 1280 Fragments arranged by size: 4676 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2492 2501 2618 2648 3275 3360 3368 Size: 393 860 9 117 30 627 85 8 Cuts at: 3368 3413 3431 3625 3728 4428 4476 4793 4947 Size: 45 18 194 103 700 48 317 154 Cuts at: 4947 5741 6338 7125 7393 1239 Size: 794 597 787 268 1524 Fragments arranged by size: 1524 860 794 787 700 627 597 393 317 268 194 154 117 103 85 48 45 30 18 9 8 EcoO109I rG'GnC_Cy Cuts at: 2028 2085 2105 2300 2928 2945 3105 3236 3434 Size: 57 20 195 628 17 160 131 198 Cuts at: 3434 3663 4935 2028 Size: 229 1272 4771 Fragments arranged by size: 4771 1272 628 229 198 195 160 131 57 20 17 EcoRI G'AATT_C Cuts at: 4987 5365 4987 Size: 378 7300 FspI TGC'GCA Cuts at: 2573 6184 2573 Size: 3611 4067 HaeII r_GCGC'y Cuts at: 1678 2022 2203 2245 2804 4822 6825 1678 Size: 344 181 42 559 2018 2003 2531 Fragments arranged by size: 2531 2018 2003 559 344 181 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2626 2658 3839 3871 4922 Size: 32 135 32 1054 32 1181 32 1051 Cuts at: 4922 4954 6319 6351 6393 6425 1373 Size: 32 1365 32 42 32 2626 Fragments arranged by size: 2626 1365 1181 1054 1051 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5022 5268 669 Size: 988 281 3084 246 3079 Fragments arranged by size: 3084 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7678 HpaI GTT'AAC Cuts at: 5268 5268 Size: 7678 KpnI G_GTAC'C Cuts at: 5003 5003 Size: 7678 MfeI C'AATT_G Cuts at: 5277 5277 Size: 7678 MluI A'CGCG_T Cuts at: 7554 7554 Size: 7678 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2960 6792 6976 1305 Size: 1655 3832 184 2007 Fragments arranged by size: 3832 2007 1655 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3175 4178 4647 10 Size: 54 1425 343 1343 1003 469 3041 Fragments arranged by size: 3041 1425 1343 1003 469 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2279 2743 4314 5636 5995 6154 518 Size: 648 1113 464 1571 1322 359 159 2042 Fragments arranged by size: 2042 1571 1322 1113 648 464 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2815 3082 3952 4038 4046 4195 4374 Size: 537 672 267 870 86 8 149 179 Cuts at: 4374 4948 5094 5771 6919 7164 1606 Size: 574 146 677 1148 245 2120 Fragments arranged by size: 2120 1148 870 677 672 574 537 267 245 179 149 146 86 8 NaeI GCC'GGC Cuts at: 3757 4190 3757 Size: 433 7245 NcoI C'CATG_G Cuts at: 513 1065 2121 2268 2995 3412 4149 4249 4425 Size: 552 1056 147 727 417 737 100 176 Cuts at: 4425 513 Size: 3766 Fragments arranged by size: 3766 1056 737 727 552 417 176 147 100 NdeI CA'TA_TG Cuts at: 387 387 Size: 7678 NgoMIV G'CCGG_C Cuts at: 3755 4188 3755 Size: 433 7245 NheI G'CTAG_C Cuts at: 1052 2386 1052 Size: 1334 6344 NotI GC'GGCC_GC Cuts at: 5125 5125 Size: 7678 NspI r_CATG'y Cuts at: 1132 1229 4816 5036 6581 1132 Size: 97 3587 220 1545 2229 Fragments arranged by size: 3587 2229 1545 220 97 PasI CC'CwG_GG Cuts at: 1348 3544 1348 Size: 2196 5482 PciI A'CATG_T Cuts at: 6577 6577 Size: 7678 PflMI CCAn_nnn'nTGG Cuts at: 1108 3001 3379 4553 1108 Size: 1893 378 1174 4233 Fragments arranged by size: 4233 1893 1174 378 PfoI T'CCnGG_A Cuts at: 3054 3054 Size: 7678 PmeI GTTT'AAAC Cuts at: 4982 4982 Size: 7678 PmlI CAC'GTG Cuts at: 3877 3877 Size: 7678 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5747 5779 7260 7292 5747 Size: 32 1481 32 6133 Fragments arranged by size: 6133 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2028 2300 2928 3663 2028 Size: 272 628 735 6043 Fragments arranged by size: 6043 735 628 272 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7678 PsiI TTA'TAA Cuts at: 5248 5248 Size: 7678 PspOMI G'GGCC_C Cuts at: 2944 3104 3235 2944 Size: 160 131 7387 Fragments arranged by size: 7387 160 131 PstI C_TGCA'G Cuts at: 830 2060 2190 2235 2829 3042 3789 3855 5030 Size: 1230 130 45 594 213 747 66 1175 Cuts at: 5030 830 Size: 3478 Fragments arranged by size: 3478 1230 1175 747 594 213 130 66 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7678 PvuII CAG'CTG Cuts at: 4195 4948 4195 Size: 753 6925 SalI G'TCGA_C Cuts at: 1936 5020 1936 Size: 3084 4594 SanDI GG'GwC_CC Cuts at: 2028 2300 2028 Size: 272 7406 SapI GCTCTTCn'nnn_ Cuts at: 3840 4701 3840 Size: 861 6817 SbfI CC_TGCA'GG Cuts at: 5030 5030 Size: 7678 ScaI AGT'ACT Cuts at: 5926 7638 5926 Size: 1712 5966 SfcI C'TryA_G Cuts at: 826 1045 2056 2186 2231 2358 2461 2825 3038 Size: 219 1011 130 45 127 103 364 213 Cuts at: 3038 3785 3851 5026 6161 6842 7033 826 Size: 747 66 1175 1135 681 191 1471 Fragments arranged by size: 1471 1175 1135 1011 747 681 364 219 213 191 130 127 103 66 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7678 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7678 SmaI CCC'GGG Cuts at: 1872 2043 3097 3506 3569 4338 5005 1872 Size: 171 1054 409 63 769 667 4545 Fragments arranged by size: 4545 1054 769 667 409 171 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 5782 6683 6945 820 Size: 197 926 3839 901 262 1553 Fragments arranged by size: 3839 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5469 493 Size: 4976 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7678 SphI G_CATG'C Cuts at: 1132 4816 5036 1132 Size: 3684 220 3774 Fragments arranged by size: 3774 3684 220 SrfI GCCC'GGGC Cuts at: 3097 3097 Size: 7678 SspI AAT'ATT Cuts at: 5 52 5602 5 Size: 47 5550 2081 Fragments arranged by size: 5550 2081 47 StuI AGG'CCT Cuts at: 1517 2229 3165 3836 4682 1517 Size: 712 936 671 846 4513 Fragments arranged by size: 4513 936 846 712 671 StyI C'CwwG_G Cuts at: 513 1065 2121 2268 2995 3412 4149 4249 4425 Size: 552 1056 147 727 417 737 100 176 Cuts at: 4425 4635 5117 513 Size: 210 482 3074 Fragments arranged by size: 3074 1056 737 727 552 482 417 210 176 147 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3688 3847 4277 4541 4834 5705 5722 Size: 1255 2108 159 430 264 293 871 17 Cuts at: 5722 5875 6060 6505 325 Size: 153 185 445 1498 Fragments arranged by size: 2108 1498 1255 871 445 430 293 264 185 159 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5924 7636 96 Size: 274 80 33 51 1443 3947 1712 138 Fragments arranged by size: 3947 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3704 3736 3796 3828 4608 4640 7638 7670 3704 Size: 32 60 32 780 32 2998 32 3712 Fragments arranged by size: 3712 2998 780 60 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2863 3698 4499 1316 Size: 1547 835 801 4495 Fragments arranged by size: 4495 1547 835 801 XbaI T'CTAG_A Cuts at: 5014 5014 Size: 7678 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2623 2785 3052 4768 2623 Size: 162 267 1716 5533 Fragments arranged by size: 5533 1716 267 162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7678 XmaI C'CCGG_G Cuts at: 1870 2041 3095 3504 3567 4336 5003 1870 Size: 171 1054 409 63 769 667 4545 Fragments arranged by size: 4545 1054 769 667 409 171 63 XmnI GAAnn'nnTTC Cuts at: 4100 5807 4100 Size: 1707 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5486 276 Size: 53 83 186 936 3952 2468 Fragments arranged by size: 3952 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SwaI