(Circular) (Six-base) MAPSORT of: pFN21AB8986.seq Check: 1283 from: 1 to: 5866 pFN21AB8986 5866 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 2481 3187 2481 Size: 706 5160 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2719 3929 5313 5581 1632 Size: 1087 1210 1384 268 1917 Fragments arranged by size: 1917 1384 1210 1087 268 AflII C'TTAA_G Cuts at: 820 1017 2867 820 Size: 197 1850 3819 Fragments arranged by size: 3819 1850 197 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 3138 1166 Size: 1972 3894 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 2752 3609 5181 2752 Size: 857 1572 3437 Fragments arranged by size: 3437 1572 857 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 2245 2408 2686 3175 3401 3553 5662 1493 Size: 752 163 278 489 226 152 2109 1697 Fragments arranged by size: 2109 1697 752 489 278 226 163 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 2639 3191 1870 Size: 73 696 552 4545 Fragments arranged by size: 4545 696 552 73 AvrII C'CTAG_G Cuts at: 2551 2551 Size: 5866 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2481 3187 4642 618 Size: 325 217 627 694 706 1455 1842 Fragments arranged by size: 1842 1455 706 694 627 325 217 BanII G_rGCy'C Cuts at: 1391 2060 3185 1391 Size: 669 1125 4072 Fragments arranged by size: 4072 1125 669 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4895 BbvCI CC'TCA_GC Cuts at: 2154 2154 Size: 5866 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BfrBI ATG'CAT Cuts at: 2981 3152 2981 Size: 171 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 4477 136 Size: 107 122 71 631 605 418 2387 1525 Fragments arranged by size: 2387 1525 631 605 418 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 3927 5083 1790 Size: 2137 1156 2573 Fragments arranged by size: 2573 2137 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2584 2883 3597 Size: 416 53 83 399 24 1167 299 714 Cuts at: 3597 4555 442 Size: 958 1753 Fragments arranged by size: 1753 1167 958 714 416 399 299 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2633 2879 4526 1239 Size: 800 594 246 1647 2579 Fragments arranged by size: 2579 1647 800 594 246 Bpu10I CC'TnA_GC Cuts at: 2154 2154 Size: 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4529 882 Size: 617 3030 2219 Fragments arranged by size: 3030 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 5860 5860 Size: 5866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2538 2556 3673 4055 Size: 53 83 186 936 1005 18 1117 382 Cuts at: 4055 4690 275 Size: 635 1451 Fragments arranged by size: 1451 1117 1005 936 635 382 186 83 53 18 BsaWI w'CCGG_w Cuts at: 2520 4299 4697 4971 5118 5529 2520 Size: 1779 398 274 147 411 2857 Fragments arranged by size: 2857 1779 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5641 Fragments arranged by size: 5641 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3064 1186 Size: 1878 3988 BseYI C'CCAG_C Cuts at: 2987 5069 5565 2987 Size: 2082 496 3288 Fragments arranged by size: 3288 2082 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2549 1163 Size: 573 813 4480 Fragments arranged by size: 4480 813 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2060 2670 3927 4012 5083 2060 Size: 610 1257 85 1071 2843 Fragments arranged by size: 2843 1257 1071 610 85 BsmI GAATG_Cn' Cuts at: 2627 3377 3470 2627 Size: 750 93 5023 Fragments arranged by size: 5023 750 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2855 2855 Size: 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2670 3185 3927 4012 5083 1391 Size: 399 270 610 515 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 610 515 399 270 85 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3221 844 Size: 2377 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3753 1394 Size: 429 1930 3507 Fragments arranged by size: 3507 1930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2680 3152 4361 4535 65 Size: 1183 1432 472 1209 174 1396 Fragments arranged by size: 1432 1396 1209 1183 472 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4510 4697 1700 Size: 110 2700 187 2869 Fragments arranged by size: 2869 2700 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 3136 3926 4938 3136 Size: 790 1012 4064 Fragments arranged by size: 4064 1012 790 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5866 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstXI CCAn_nnnn'nTGG Cuts at: 2236 2497 2578 3023 2236 Size: 261 81 445 5079 Fragments arranged by size: 5079 445 261 81 BstYI r'GATC_y Cuts at: 1972 2908 3196 3563 3948 3965 5417 5503 5515 Size: 936 288 367 385 17 1452 86 12 Cuts at: 5515 5861 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 936 385 367 346 288 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2100 513 Size: 552 1035 4279 Fragments arranged by size: 4279 1035 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2180 4873 809 Size: 507 864 2693 1802 Fragments arranged by size: 2693 1802 864 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 2487 2499 Size: 54 1425 50 275 18 252 405 12 Cuts at: 2499 3313 4202 5831 8 Size: 814 889 1629 43 Fragments arranged by size: 1629 1425 889 814 405 275 252 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2831 3796 1353 Size: 695 783 965 3423 Fragments arranged by size: 3423 965 783 695 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2058 2058 Size: 5866 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2633 2719 2879 3929 4526 5313 Size: 393 407 594 86 160 1050 597 787 Cuts at: 5313 5581 1239 Size: 268 1524 Fragments arranged by size: 1524 1050 787 597 594 407 393 268 160 86 EcoO109I rG'GnC_Cy Cuts at: 2091 2386 2644 2091 Size: 295 258 5313 Fragments arranged by size: 5313 295 258 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 3055 5013 1678 Size: 1377 1958 2531 Fragments arranged by size: 2531 1958 1377 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2893 2925 3059 3091 4507 Size: 32 135 32 1321 32 134 32 1416 Cuts at: 4507 4539 4581 4613 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1416 1321 135 134 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3210 3456 669 Size: 988 281 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 2485 3191 2485 Size: 706 5160 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4980 5164 1305 Size: 3675 184 2007 Fragments arranged by size: 3675 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2084 2489 10 Size: 54 1425 343 252 405 3387 Fragments arranged by size: 3387 1425 405 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2576 3021 3105 3138 3824 4183 4342 Size: 648 1410 445 84 33 686 359 159 Cuts at: 4342 518 Size: 2042 Fragments arranged by size: 2042 1410 686 648 445 359 159 84 33 MspA1I CmG'CkG Cuts at: 1606 3282 3959 5107 5352 1606 Size: 1676 677 1148 245 2120 Fragments arranged by size: 2120 1676 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2100 513 Size: 552 1035 4279 Fragments arranged by size: 4279 1035 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NsiI A_TGCA'T Cuts at: 2983 3154 2983 Size: 171 5695 NspI r_CATG'y Cuts at: 1132 1229 3224 4769 1132 Size: 97 1995 1545 2229 Fragments arranged by size: 2229 1995 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5866 PfoI T'CCnGG_A Cuts at: 2649 2856 2649 Size: 207 5659 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2644 2644 Size: 5866 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 RsrII CG'GwC_CG Cuts at: 2523 2523 Size: 5866 SacI G_AGCT'C Cuts at: 2060 2060 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SapI GCTCTTCn'nnn_ Cuts at: 2048 2048 Size: 5866 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 3214 4349 5030 5221 826 Size: 219 2169 1135 681 191 1471 Fragments arranged by size: 2169 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2090 2090 Size: 5866 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2867 3970 4871 5133 820 Size: 197 926 924 1103 901 262 1553 Fragments arranged by size: 1553 1103 926 924 901 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 2159 1517 Size: 642 5224 StyI C'CwwG_G Cuts at: 513 1065 2100 2551 2626 3305 513 Size: 552 1035 451 75 679 3074 Fragments arranged by size: 3074 1035 679 552 451 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2061 3893 3910 4063 4248 4693 325 Size: 1255 481 1832 17 153 185 445 1498 Fragments arranged by size: 1832 1498 1255 481 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4112 5824 96 Size: 274 80 33 51 1443 2135 1712 138 Fragments arranged by size: 2135 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 3140 2236 Size: 904 4962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII SacII SanDI SexAI SfoI SrfI SwaI