Restriction map for
pFN21AB8434
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AvaII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI HgaI Hin1I
HindIII HpaI KasI Ksp632I MaeIII
MamI MboII MfeI MluI MroI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 389 GGTACC G^GTACC
AfaI 390 GTAC GT^AC
AgeI 246 ACCGGT A^CCGGT
Asp718I 389 GGTACC G^GTACC
AvaI 263 CCCGGG C^[CT]CG[GA]G
BalI 53 TGGCCA TGG^CCA
BamHI 114 GGATCC G^GATCC
BanI 389 GGTACC G^G[CT][GA]CC
BanII 211 GAGCCC G[GA]GC[CT]^C
BfaI 76 CTAG C^TAG
BglI 175 GCCCGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 211 GAGCCC G[AGT]GC[ACT]^C
BsaWI 246 ACCGGT [AT]^CCGG[AT]
BsgI 295 CTGCAC GTGCAG(16/14)
BsiEI 244 CGACCG CG[GA][CT]^CG
BsoBI 263 CCCGGG C^[CT]CG[GA]G
Bsp1286I 211 GAGCCC G[AGT]GC[ACT]^C
BspHI 158 TCATGA T^CATGA
BspMI 392 ACCTGC ACCTGC(4/8)
BsrBI 364 GAGCGG CCGCTC(-3/-3)
BsrI 26 CCAGT ACTGG(1/-1)
BssHII 144 GCGCGC G^CGCGC
BstAPI 223 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 114 GGATCC [GA]^GATC[CT]
HincII 127 GTCAAC GT[CT]^[GA]AC
HindII 127 GTCAAC GT[CT]^[GA]AC
HinfI 125 GAGTC G^A[ACGT]TC
HphI 239 TCACC GGTGA(8/7)
KpnI 389 GGTACC GGTAC^C
KspI 62 CCGCGG CCGC^GG
MaeI 76 CTAG C^TAG
MflI 114 GGATCC [GA]^GATC[CT]
MluNI 53 TGGCCA TGG^CCA
MscI 53 TGGCCA TGG^CCA
MspA1I 62 CCGCGG C[AC]G^C[GT]G
NaeI 106 GCCGGC GCC^GGC
NgoMIV 106 GCCGGC G^CCGGC
NspI 20 GCATGC [GA]CATG^[CT]
PinAI 246 ACCGGT A^CCGGT
PleI 125 GAGTC GAGTC(4/5)
RcaI 158 TCATGA T^CATGA
RsaI 390 GTAC GT^AC
SacII 62 CCGCGG CCGC^GG
SmaI 263 CCCGGG CCC^GGG
SpeI 75 ACTAGT A^CTAGT
SphI 20 GCATGC GCATG^C
TspRI 27 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 114 GGATCC [GA]^GATC[CT]
XmaCI 263 CCCGGG C^CCGGG
XmaI 263 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AluI 95 AGCT AG^CT
188 AGCT
AlwNI 94 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
224 CAGACCCTG
AviII 220 TGCGCA TGC^GCA
395 TGCGCA
BseRI 7 GAGGAG GAGGAG(10/8)
361 GAGGAG
BsmFI 72 GGGAC GGGAC(10/14)
410 GGGAC
BsrFI 106 GCCGGC [GA]^CCGG[CT]
246 ACCGGT
Cfr10I 106 GCCGGC [GA]^CCGG[CT]
246 ACCGGT
Cfr13I 302 GGGCC G^G[ACGT]CC
369 GGGCC
DpnII 115 GATC ^GATC
150 GATC
EaeI 53 TGGCCA [CT]^GGCC[GA]
356 TGGCCG
FspI 220 TGCGCA TGC^GCA
395 TGCGCA
HaeII 58 AGCGCC [GA]GCGC^[CT]
172 AGCGCC
MaeII 320 ACGT A^CGT
380 ACGT
MboI 115 GATC ^GATC
150 GATC
MslI 159 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
282 CACGCTGGTG
NdeII 115 GATC ^GATC
150 GATC
Sau3AI 115 GATC ^GATC
150 GATC
Sau96I 302 GGGCC G^G[ACGT]CC
369 GGGCC
SfaNI 256 GATGC GCATC(5/9)
328 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AlwI BbvI BcnI
BsaJI BsiYI BslI BssKI BstNI
BstUI Cac8I CfoI DsaI EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV ScrFI TseI