Restriction map for
pFN21AF0137
(Linear) (Six-base) MAPSORT of: FLJ00137.seq Check: 5439 from: 1 to: 4524
FLJ00137.orf
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3594 4524
Size: 3594 930
AccI GT'mk_AC
Cuts at: 0 2049 3339 4524
Size: 2049 1290 1185
Fragments arranged by size:
2049 1290 1185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 829 954 1861 3085 3486 3989 3998
Size: 641 188 125 907 1224 401 503 9
Cuts at: 3998 4145 4453 4524
Size: 147 308 71
Fragments arranged by size:
1224 907 641 503 401 308 188 147
125 71 9
AfeI AGC'GCT
Cuts at: 0 1292 4524
Size: 1292 3232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 302 334 4524
Size: 302 32 4190
Fragments arranged by size:
4190 302 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2314 2866 3546 3600 3902 4524
Size: 2314 552 680 54 302 622
Fragments arranged by size:
2314 680 622 552 302 54
ApaI G_GGCC'C
Cuts at: 0 127 4524
Size: 127 4397
ApaLI G'TGCA_C
Cuts at: 0 1831 2119 4524
Size: 1831 288 2405
Fragments arranged by size:
2405 1831 288
ApoI r'AATT_y
Cuts at: 0 1257 3859 3891 4339 4524
Size: 1257 2602 32 448 185
Fragments arranged by size:
2602 1257 448 185 32
AseI AT'TA_AT
Cuts at: 0 2459 4524
Size: 2459 2065
AvaI C'yCGr_G
Cuts at: 0 70 126 356 4524
Size: 70 56 230 4168
Fragments arranged by size:
4168 230 70 56
BamHI G'GATC_C
Cuts at: 0 2079 4524
Size: 2079 2445
BanI G'GyrC_C
Cuts at: 0 1301 2856 3451 4524
Size: 1301 1555 595 1073
Fragments arranged by size:
1555 1301 1073 595
BanII G_rGCy'C
Cuts at: 0 127 1892 1945 2021 3312 4076 4466 4524
Size: 127 1765 53 76 1291 764 390 58
Fragments arranged by size:
1765 1291 764 390 127 76 58 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 99 1417 1849 4506 4524
Size: 99 1318 432 2657 18
Fragments arranged by size:
2657 1318 432 99 18
BbvCI CC'TCA_GC
Cuts at: 0 3079 4524
Size: 3079 1445
BglI GCCn_nnn'nGGC
Cuts at: 0 83 4008 4524
Size: 83 3925 516
Fragments arranged by size:
3925 516 83
BglII A'GATC_T
Cuts at: 0 2790 4524
Size: 2790 1734
BlpI GC'TnA_GC
Cuts at: 0 1323 3637 4524
Size: 1323 2314 887
Fragments arranged by size:
2314 1323 887
Bme1580I G_kGCm'C
Cuts at: 0 71 127 1835 2123 3276 3454 4524
Size: 71 56 1708 288 1153 178 1070
Fragments arranged by size:
1708 1153 1070 288 178 71 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 3052 3309 4524
Size: 3052 257 1215
Fragments arranged by size:
3052 1215 257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2549 4524
Size: 2549 1975
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 780 1856 2372 2405 2610 2791 3391
Size: 686 94 1076 516 33 205 181 600
Cuts at: 3391 3412 3861 4402 4524
Size: 21 449 541 122
Fragments arranged by size:
1076 686 600 541 516 449 205 181
122 94 33 21
Bpu10I CC'TnA_GC
Cuts at: 0 3079 3518 4524
Size: 3079 439 1006
Fragments arranged by size:
3079 1006 439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2147 2265 2977 4047 4524
Size: 2147 118 712 1070 477
Fragments arranged by size:
2147 1070 712 477 118
BsaI GGTCTCn'nnnn_
Cuts at: 0 204 356 2075 3875 4129 4524
Size: 204 152 1719 1800 254 395
Fragments arranged by size:
1800 1719 395 254 204 152
BsaAI yAC'GTr
Cuts at: 0 1830 4524
Size: 1830 2694
BsaHI Gr'CG_yC
Cuts at: 0 3849 4524
Size: 3849 675
BsaWI w'CCGG_w
Cuts at: 0 1895 3695 4215 4524
Size: 1895 1800 520 309
Fragments arranged by size:
1895 1800 520 309
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 164 194 302 332 1354 1384 3364 3394
Size: 164 30 108 30 1022 30 1980 30
Cuts at: 3394 4524
Size: 1130
Fragments arranged by size:
1980 1130 1022 164 108 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 344 833 1478 1520 2150 2362 2596 3414
Size: 344 489 645 42 630 212 234 818
Cuts at: 3414 3417 4524
Size: 3 1107
Fragments arranged by size:
1107 818 645 630 489 344 234 212
42 3
BseYI C'CCAG_C
Cuts at: 0 874 3083 4524
Size: 874 2209 1441
Fragments arranged by size:
2209 1441 874
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1131 2400 3691 4524
Size: 1131 1269 1291 833
Fragments arranged by size:
1291 1269 1131 833
BsiHKAI G_wGCw'C
Cuts at: 0 1835 2123 4076 4466 4524
Size: 1835 288 1953 390 58
Fragments arranged by size:
1953 1835 390 288 58
BsmI GAATG_Cn'
Cuts at: 0 690 4058 4524
Size: 690 3368 466
Fragments arranged by size:
3368 690 466
BsmBI CGTCTCn'nnnn_
Cuts at: 0 945 4524
Size: 945 3579
Bsp1286I G_dGCh'C
Cuts at: 0 71 127 1835 1892 1945 2021 2123 3276
Size: 71 56 1708 57 53 76 102 1153
Cuts at: 3276 3312 3454 4076 4466 4524
Size: 36 142 622 390 58
Fragments arranged by size:
1708 1153 622 390 142 102 76 71
58 57 56 53 36
BspEI T'CCGG_A
Cuts at: 0 3695 4524
Size: 3695 829
BspHI T'CATG_A
Cuts at: 0 486 1761 1950 4524
Size: 486 1275 189 2574
Fragments arranged by size:
2574 1275 486 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3594 4524
Size: 3594 930
BsrDI GCAATG_nn'
Cuts at: 0 934 1668 4524
Size: 934 734 2856
Fragments arranged by size:
2856 934 734
BsrGI T'GTAC_A
Cuts at: 0 1611 4524
Size: 1611 2913
BstBI TT'CG_AA
Cuts at: 0 753 4524
Size: 753 3771
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3174 3390 4423 4524
Size: 3174 216 1033 101
Fragments arranged by size:
3174 1033 216 101
BstYI r'GATC_y
Cuts at: 0 933 2079 2572 2790 4524
Size: 933 1146 493 218 1734
Fragments arranged by size:
1734 1146 933 493 218
Bsu36I CC'TnA_GG
Cuts at: 0 2210 2430 4524
Size: 2210 220 2094
Fragments arranged by size:
2210 2094 220
BtgI C'CryG_G
Cuts at: 0 13 4524
Size: 13 4511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 74 4524
Size: 74 4450
BtsI GCAGTG_nn'
Cuts at: 0 3491 3638 3956 4503 4524
Size: 3491 147 318 547 21
Fragments arranged by size:
3491 547 318 147 21
ClaI AT'CG_AT
Cuts at: 0 2269 4524
Size: 2269 2255
DraIII CAC_nnn'GTG
Cuts at: 0 2970 4524
Size: 2970 1554
DrdI GACnn_nn'nnGTC
Cuts at: 0 2045 4524
Size: 2045 2479
EaeI y'GGCC_r
Cuts at: 0 1184 1916 2748 3108 3176 3290 4524
Size: 1184 732 832 360 68 114 1234
Fragments arranged by size:
1234 1184 832 732 360 114 68
EarI CTCTTCn'nnn_
Cuts at: 0 805 810 973 1167 2009 2518 3487 4524
Size: 805 5 163 194 842 509 969 1037
Fragments arranged by size:
1037 969 842 805 509 194 163 5
EcoICRI GAG'CTC
Cuts at: 0 4074 4464 4524
Size: 4074 390 60
Fragments arranged by size:
4074 390 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 686 780 829 954 1856 1861 2372
Size: 641 45 94 49 125 902 5 511
Cuts at: 2372 2405 2610 2791 3085 3391 3412 3486 3861
Size: 33 205 181 294 306 21 74 375
Cuts at: 3861 3989 3998 4145 4402 4453 4524
Size: 128 9 147 257 51 71
Fragments arranged by size:
902 641 511 375 306 294 257 205
181 147 128 125 94 74 71 51
49 45 33 21 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2162 4524
Size: 2162 2362
EcoO109I rG'GnC_Cy
Cuts at: 0 75 2311 3054 3460 4412 4524
Size: 75 2236 743 406 952 112
Fragments arranged by size:
2236 952 743 406 112 75
EcoRI G'AATT_C
Cuts at: 0 3859 4524
Size: 3859 665
EcoRV GAT'ATC
Cuts at: 0 335 4524
Size: 335 4189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 868 2259 2602 3301 3516 4092 4483 4524
Size: 868 1391 343 699 215 576 391 41
Fragments arranged by size:
1391 868 699 576 391 343 215 41
HaeII r_GCGC'y
Cuts at: 0 852 1294 3679 4524
Size: 852 442 2385 845
Fragments arranged by size:
2385 852 845 442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 1162 1194 1354 1386 1932 1964
Size: 619 32 511 32 160 32 546 32
Cuts at: 1964 2557 2589 3181 3213 3232 3264 3364 3396
Size: 593 32 592 32 19 32 100 32
Cuts at: 3396 3781 3813 4524
Size: 385 32 711
Fragments arranged by size:
711 619 593 592 546 511 385 160
100 32 32 32 32 32 32 32
32 32 19
HincII GTy'rAC
Cuts at: 0 860 2050 4174 4301 4524
Size: 860 1190 2124 127 223
Fragments arranged by size:
2124 1190 860 223 127
HindIII A'AGCT_T
Cuts at: 0 1540 1735 2897 4524
Size: 1540 195 1162 1627
Fragments arranged by size:
1627 1540 1162 195
HpaI GTT'AAC
Cuts at: 0 4301 4524
Size: 4301 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1397 1609 1746 1835 4179 4524
Size: 281 1116 212 137 89 2344 345
Fragments arranged by size:
2344 1116 345 281 212 137 89
MscI TGG'CCA
Cuts at: 0 1186 2750 3110 3178 3292 4524
Size: 1186 1564 360 68 114 1232
Fragments arranged by size:
1564 1232 1186 360 114 68
MslI CAynn'nnrTG
Cuts at: 0 526 584 1766 1828 1952 2137 3724 4524
Size: 526 58 1182 62 124 185 1587 800
Fragments arranged by size:
1587 1182 800 526 185 124 62 58
MspA1I CmG'CkG
Cuts at: 0 15 53 894 1050 1115 1499 2392 2866
Size: 15 38 841 156 65 384 893 474
Cuts at: 2866 2913 3083 3327 4524
Size: 47 170 244 1197
Fragments arranged by size:
1197 893 841 474 384 244 170 156
65 47 38 15
NspI r_CATG'y
Cuts at: 0 316 4093 4524
Size: 316 3777 431
Fragments arranged by size:
3777 431 316
PflMI CCAn_nnn'nTGG
Cuts at: 0 605 1754 2866 3299 3648 3902 4524
Size: 605 1149 1112 433 349 254 622
Fragments arranged by size:
1149 1112 622 605 433 349 254
PfoI T'CCnGG_A
Cuts at: 0 2009 4524
Size: 2009 2515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 302 334 4524
Size: 302 32 4190
Fragments arranged by size:
4190 302 32
PpuMI rG'GwC_Cy
Cuts at: 0 2311 3054 4412 4524
Size: 2311 743 1358 112
Fragments arranged by size:
2311 1358 743 112
PshAI GACnn'nnGTC
Cuts at: 0 103 4524
Size: 103 4421
PspOMI G'GGCC_C
Cuts at: 0 123 4524
Size: 123 4401
PspXI vC'TCGA_Gb
Cuts at: 0 356 4524
Size: 356 4168
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3544 3576 4524
Size: 3544 32 948
Fragments arranged by size:
3544 948 32
PstI C_TGCA'G
Cuts at: 0 58 2817 3216 3326 3486 3551 4246 4524
Size: 58 2759 399 110 160 65 695 278
Fragments arranged by size:
2759 695 399 278 160 110 65 58
PvuII CAG'CTG
Cuts at: 0 894 1050 1115 1499 2866 2913 3083 3327
Size: 894 156 65 384 1367 47 170 244
Cuts at: 3327 4524
Size: 1197
Fragments arranged by size:
1367 1197 894 384 244 170 156 65
47
SacI G_AGCT'C
Cuts at: 0 4076 4466 4524
Size: 4076 390 58
Fragments arranged by size:
4076 390 58
SacII CC_GC'GG
Cuts at: 0 16 4524
Size: 16 4508
SalI G'TCGA_C
Cuts at: 0 2048 4524
Size: 2048 2476
SapI GCTCTTCn'nnn_
Cuts at: 0 2009 3487 4524
Size: 2009 1478 1037
Fragments arranged by size:
2009 1478 1037
ScaI AGT'ACT
Cuts at: 0 4062 4524
Size: 4062 462
SfcI C'TryA_G
Cuts at: 0 54 689 2813 3119 3212 3322 3340 3482
Size: 54 635 2124 306 93 110 18 142
Cuts at: 3482 3547 4242 4524
Size: 65 695 282
Fragments arranged by size:
2124 695 635 306 282 142 110 93
65 54 18
SmaI CCC'GGG
Cuts at: 0 72 128 4524
Size: 72 56 4396
Fragments arranged by size:
4396 72 56
SmlI C'TyrA_G
Cuts at: 0 356 2162 2244 2992 4026 4524
Size: 356 1806 82 748 1034 498
Fragments arranged by size:
1806 1034 748 498 356 82
SphI G_CATG'C
Cuts at: 0 4093 4524
Size: 4093 431
SrfI GCCC'GGGC
Cuts at: 0 128 4524
Size: 128 4396
SspI AAT'ATT
Cuts at: 0 1685 4524
Size: 1685 2839
StuI AGG'CCT
Cuts at: 0 3832 4011 4360 4524
Size: 3832 179 349 164
Fragments arranged by size:
3832 349 179 164
StyI C'CwwG_G
Cuts at: 0 1103 1131 1280 1310 3032 4139 4524
Size: 1103 28 149 30 1722 1107 385
Fragments arranged by size:
1722 1107 1103 385 149 30 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1319 3291 4524
Size: 1319 1972 1233
Fragments arranged by size:
1972 1319 1233
TatI w'GTAC_w
Cuts at: 0 233 1611 1714 2554 3737 4060 4524
Size: 233 1378 103 840 1183 323 464
Fragments arranged by size:
1378 1183 840 464 323 233 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 164 196 1530 1562 2941 2973 4524
Size: 164 32 1334 32 1379 32 1551
Fragments arranged by size:
1551 1379 1334 164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3058 4524
Size: 3058 1466
XbaI T'CTAG_A
Cuts at: 0 769 2398 4524
Size: 769 1629 2126
Fragments arranged by size:
2126 1629 769
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3300 4524
Size: 3300 1224
XhoI C'TCGA_G
Cuts at: 0 356 4524
Size: 356 4168
XmaI C'CCGG_G
Cuts at: 0 70 126 4524
Size: 70 56 4398
Fragments arranged by size:
4398 70 56
XmnI GAAnn'nnTTC
Cuts at: 0 560 1685 2669 3536 4524
Size: 560 1125 984 867 988
Fragments arranged by size:
1125 988 984 867 560
Enzymes that do cut:
AarI AccI AcuI AfeI AloI AlwNI ApaI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII HpaI MmeI MscI MslI MspA1I NspI PflMI
PfoI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuII SacI SacII SalI SapI ScaI SfcI SmaI
SmlI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AscI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BmgBI BmtI BsaBI BsiEI BsiWI BsrBI
BsrFI BssHII BssSI BstAPI BstEII BstZ17I CspCI DraI
EagI EciI FseI FspI FspAI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PsiI
PvuI RsrII SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SwaI ZraI