Restriction map for
pFN21AB6277
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BcgI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsiEI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstUI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoT22I FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NciI NheI NotI
NruI NsiI PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 448 ACACGT A^C[GA][CT]GT
Alw44I 1625 GTGCAC G^TGCAC
AocI 154 CCTGAGG CC^T[ACGT]AGG
ApaLI 1625 GTGCAC G^TGCAC
BbrPI 449 CACGTG CAC^GTG
BbsI 1372 GAAGAC GAAGAC(2/6)
BbvCI 1897 GCTGAGG CCTCAGC(-5/-2)
BglII 2262 AGATCT A^GATCT
BpuAI 1372 GAAGAC GAAGAC(2/6)
BsaI 1198 GGTCTC GGTCTC(1/5)
BsaWI 1720 ACCGGA [AT]^CCGG[AT]
BsiWI 1648 CGTACG C^GTACG
BsrDI 259 GCAATG GCAATG(2/0)
BsrFI 1062 GCCGGC [GA]^CCGG[CT]
BstBI 1541 TTCGAA TT^CGAA
BstEII 1248 GGTCACC G^GT[ACGT]ACC
BstPI 1248 GGTCACC G^GT[ACGT]ACC
Bsu36I 154 CCTGAGG CC^T[ACGT]AGG
CfoI 1644 GCGC GCG^C
Cfr10I 1062 GCCGGC [GA]^CCGG[CT]
Eco81I 154 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1248 GGTCACC G^GT[ACGT]ACC
EcoRI 1265 GAATTC G^AATTC
EcoRV 2123 GATATC GAT^ATC
FauI 1561 CCCGC CCCGC(4/6)
HaeII 1643 AGCGCC [GA]GCGC^[CT]
HhaI 1644 GCGC GCG^C
HinP1I 1644 GCGC G^CGC
HincII 1121 GTCAAC GT[CT]^[GA]AC
HindII 1121 GTCAAC GT[CT]^[GA]AC
NaeI 1062 GCCGGC GCC^GGC
NdeI 1958 CATATG CA^TATG
NgoMIV 1062 GCCGGC G^CCGGC
NspV 1541 TTCGAA TT^CGAA
PaeR7I 266 CTCGAG C^TCGAG
PmaCI 449 CACGTG CAC^GTG
PmlI 449 CACGTG CAC^GTG
SacI 269 GAGCTC GAGCT^C
SapI 1399 GCTCTTC GCTCTTC(1/4)
SexAI 491 ACCAGGT A^CC[AT]GGT
SfuI 1541 TTCGAA TT^CGAA
SnaBI 596 TACGTA TAC^GTA
SpeI 1605 ACTAGT A^CTAGT
XcmI 1498 CCACCATCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 266 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 101 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1265 GAATTCTTTC
AvaI 266 CTCGAG C^[CT]CG[GA]G
1742 CTCGGG
BanII 269 GAGCTC G[GA]GC[CT]^C
1724 GAGCCC
BciVI 171 GGATAC GTATCC(6/5)
1635 GGATAC
BpmI 2300 CTCCAG CTGGAG(16/14)
701 CTGGAG
BsaAI 449 CACGTG [CT]AC^GT[GA]
596 TACGTA
BseRI 572 CTCCTC GAGGAG(10/8)
1913 CTCCTC
BsgI 1256 GTGCAG GTGCAG(16/14)
1798 GTGCAG
BsoBI 266 CTCGAG C^[CT]CG[GA]G
1742 CTCGGG
BstAPI 436 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1564 GCAAATCATGC
DsaI 38 CCATGG C^C[GA][CT]GG
1655 CCATGG
HapII 1063 CCGG C^CGG
1721 CCGG
HpaII 1063 CCGG C^CGG
1721 CCGG
MspI 1063 CCGG C^CGG
1721 CCGG
NcoI 38 CCATGG C^CATGG
1655 CCATGG
SphI 317 GCATGC GCATG^C
2271 GCATGC
TaqI 267 TCGA T^CGA
1542 TCGA
TthHB8I 267 TCGA T^CGA
1542 TCGA
XmnI 101 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1265 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BbvI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII