(Circular) (Six-base) MAPSORT of: pFN21AB6277.seq Check: 5761 from: 1 to: 7048 pFN21AB6277 7048 bp With 182 enzymes: SgfI * May 6, 2010 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4858 278 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4391 1937 Size: 2454 4594 Acc65I G'GTAC_C Cuts at: 4369 4369 Size: 7048 AclI AA'CG_TT Cuts at: 1563 5175 5548 1563 Size: 3612 373 3063 Fragments arranged by size: 3612 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3056 4036 5111 6495 6763 1632 Size: 1424 980 1075 1384 268 1917 Fragments arranged by size: 1917 1424 1384 1075 980 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6851 AflIII A'CryG_T Cuts at: 1203 2453 5947 6924 1203 Size: 1250 3494 977 1327 Fragments arranged by size: 3494 1327 1250 977 AgeI A'CCGG_T Cuts at: 5879 5879 Size: 7048 AhdI GACnn_n'nnGTC Cuts at: 5777 5777 Size: 7048 AleI CACnn'nnGTG Cuts at: 1166 4167 4236 1166 Size: 3001 69 3978 Fragments arranged by size: 3978 3001 69 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7048 AlwNI CAG_nnn'CTG Cuts at: 2398 3176 3229 3974 4791 6363 2398 Size: 778 53 745 817 1572 3083 Fragments arranged by size: 3083 1572 817 778 745 53 ApaLI G'TGCA_C Cuts at: 3630 5105 6261 3630 Size: 1475 1156 4417 Fragments arranged by size: 4417 1475 1156 ApoI r'AATT_y Cuts at: 1493 2879 2910 3270 4357 4583 4735 6844 1493 Size: 1386 31 360 1087 226 152 2109 1697 Fragments arranged by size: 2109 1697 1386 1087 360 226 152 31 AseI AT'TA_AT Cuts at: 160 1032 5602 160 Size: 872 4570 1606 Fragments arranged by size: 4570 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 AvaI C'yCGr_G Cuts at: 1870 1943 2271 3747 4373 1870 Size: 73 328 1476 626 4545 Fragments arranged by size: 4545 1476 626 328 73 BamHI G'GATC_C Cuts at: 4378 4745 4378 Size: 367 6681 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 3596 4106 4369 5824 Size: 325 217 627 330 1479 510 263 1455 Cuts at: 5824 618 Size: 1842 Fragments arranged by size: 1842 1479 1455 627 510 330 325 263 217 BanII G_rGCy'C Cuts at: 1391 2278 3733 4367 1391 Size: 887 1455 634 4072 Fragments arranged by size: 4072 1455 887 634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3384 928 Size: 971 1485 4592 Fragments arranged by size: 4592 1485 971 BbvCI CC'TCA_GC Cuts at: 3903 3903 Size: 7048 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5239 5273 1903 Size: 34 3302 34 3678 Fragments arranged by size: 3678 3302 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2170 3634 4940 6150 2170 Size: 1464 1306 1210 3068 Fragments arranged by size: 3068 1464 1306 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7048 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5659 136 Size: 107 122 71 631 605 3987 1525 Fragments arranged by size: 3987 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4267 7043 4267 Size: 2776 4272 BlpI GC'TnA_GC Cuts at: 4465 4465 Size: 7048 Bme1580I G_kGCm'C Cuts at: 1790 3634 4111 5109 6265 1790 Size: 1844 477 998 1156 2573 Fragments arranged by size: 2573 1844 1156 998 477 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7048 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4779 5737 442 Size: 416 53 83 399 24 3362 958 1753 Fragments arranged by size: 3362 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7048 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2727 4290 5708 1239 Size: 1488 1563 1418 2579 Fragments arranged by size: 2579 1563 1488 1418 Bpu10I CC'TnA_GC Cuts at: 3870 3903 3870 Size: 33 7015 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1998 2214 5173 6038 6336 1998 Size: 216 2959 865 298 2710 Fragments arranged by size: 2959 2710 865 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3209 5711 882 Size: 617 1710 2502 2219 Fragments arranged by size: 2502 2219 1710 617 BsaAI yAC'GTr Cuts at: 493 1204 2456 2603 4839 493 Size: 711 1252 147 2236 2702 Fragments arranged by size: 2702 2236 1252 711 147 BsaBI GATnn'nnATC Cuts at: 7042 7042 Size: 7048 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4855 5237 5872 275 Size: 53 83 186 936 3322 382 635 1451 Fragments arranged by size: 3322 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3725 5481 5879 6153 6300 6711 3725 Size: 1756 398 274 147 411 4062 Fragments arranged by size: 4062 1756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2116 2146 1178 Size: 30 165 30 713 30 6080 Fragments arranged by size: 6080 713 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2568 3909 1186 Size: 1382 1341 4325 Fragments arranged by size: 4325 1382 1341 BseYI C'CCAG_C Cuts at: 2648 3645 6251 6747 2648 Size: 997 2606 496 2949 Fragments arranged by size: 2949 2606 997 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3282 3824 1163 Size: 573 1546 542 4387 Fragments arranged by size: 4387 1546 573 542 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4498 5259 6287 1540 Size: 275 186 2497 761 1028 2301 Fragments arranged by size: 2497 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2278 3406 3634 5109 5194 6265 2278 Size: 1128 228 1475 85 1071 3061 Fragments arranged by size: 3061 1475 1128 1071 228 85 BsiWI C'GTAC_G Cuts at: 3653 3653 Size: 7048 BsmI GAATG_Cn' Cuts at: 2712 3096 3457 4559 4652 2712 Size: 384 361 1102 93 5108 Fragments arranged by size: 5108 1102 384 361 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2278 3406 3634 3733 4111 4367 5109 Size: 399 488 1128 228 99 378 256 742 Cuts at: 5109 5194 6265 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1128 1071 742 488 399 378 256 228 99 85 BspEI T'CCGG_A Cuts at: 6711 6711 Size: 7048 BspHI T'CATG_A Cuts at: 4937 6667 4937 Size: 1730 5318 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4403 844 Size: 3559 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4935 1394 Size: 429 3112 3507 Fragments arranged by size: 3507 3112 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2271 5543 5717 65 Size: 1183 1023 3272 174 1396 Fragments arranged by size: 3272 1396 1183 1023 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3067 5692 5879 1700 Size: 110 1257 2625 187 2869 Fragments arranged by size: 2869 2625 1257 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7048 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7048 BssSI C'ACGA_G Cuts at: 5108 6120 5108 Size: 1012 6036 BstAPI GCAn_nnn'nTGC Cuts at: 794 2447 3575 794 Size: 1653 1128 4267 Fragments arranged by size: 4267 1653 1128 BstBI TT'CG_AA Cuts at: 3547 5852 3547 Size: 2305 4743 BstEII G'GTnAC_C Cuts at: 1184 3253 1184 Size: 2069 4979 BstXI CCAn_nnnn'nTGG Cuts at: 3329 3513 4071 4235 3329 Size: 184 558 164 6142 Fragments arranged by size: 6142 558 184 164 BstYI r'GATC_y Cuts at: 1972 2328 3118 4267 4378 4745 5130 5147 6599 Size: 356 790 1149 111 367 385 17 1452 Cuts at: 6599 6685 6697 7043 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1149 790 385 367 356 346 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2160 2160 Size: 7048 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 3660 513 Size: 552 939 39 1617 3901 Fragments arranged by size: 3901 1617 939 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6309 Fragments arranged by size: 6309 683 56 BtsI GCAGTG_nn' Cuts at: 2826 4564 5358 5378 2826 Size: 1738 794 20 4496 Fragments arranged by size: 4496 1738 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7048 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7013 DraI TTT'AAA Cuts at: 4352 4352 Size: 7048 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6055 809 Size: 507 4739 1802 Fragments arranged by size: 4739 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2691 4177 Size: 54 1425 50 275 18 210 651 1486 Cuts at: 4177 4225 4495 5384 7013 8 Size: 48 270 889 1629 43 Fragments arranged by size: 1629 1486 1425 889 651 275 270 210 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4495 1537 Size: 275 2683 4090 Fragments arranged by size: 4090 2683 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 3411 4055 4978 1353 Size: 807 1251 644 923 3423 Fragments arranged by size: 3423 1251 923 807 644 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5635 6009 6155 387 Size: 1064 138 4046 374 146 1280 Fragments arranged by size: 4046 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2276 2276 Size: 7048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2727 3056 4036 4290 5111 5708 6495 Size: 393 1095 329 980 254 821 597 787 Cuts at: 6495 6763 1239 Size: 268 1524 Fragments arranged by size: 1524 1095 980 821 787 597 393 329 268 254 EcoO109I rG'GnC_Cy Cuts at: 2652 2991 3853 4285 2652 Size: 339 862 432 5415 Fragments arranged by size: 5415 862 432 339 EcoRI G'AATT_C Cuts at: 3270 4357 4735 3270 Size: 1087 378 5583 Fragments arranged by size: 5583 1087 378 EcoRV GAT'ATC Cuts at: 4130 4130 Size: 7048 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3224 3224 Size: 7048 FspI TGC'GCA Cuts at: 5554 5554 Size: 7048 HaeII r_GCGC'y Cuts at: 1678 3652 6195 1678 Size: 1974 2543 2531 Fragments arranged by size: 2543 2531 1974 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2260 2292 2413 2445 2550 Size: 32 135 32 688 32 121 32 105 Cuts at: 2550 2582 3187 3219 3535 3567 4030 4062 4072 Size: 32 605 32 316 32 463 32 10 Cuts at: 4072 4104 5689 5721 5763 5795 1373 Size: 32 1585 32 42 32 2626 Fragments arranged by size: 2626 1585 688 605 463 316 135 121 105 42 32 32 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3128 4392 4638 669 Size: 988 281 1190 1264 246 3079 Fragments arranged by size: 3079 1264 1190 988 281 246 HindIII A'AGCT_T Cuts at: 748 2550 2970 3096 748 Size: 1802 420 126 4700 Fragments arranged by size: 4700 1802 420 126 HpaI GTT'AAC Cuts at: 4638 4638 Size: 7048 KpnI G_GTAC'C Cuts at: 4373 4373 Size: 7048 MfeI C'AATT_G Cuts at: 4647 4647 Size: 7048 MluI A'CGCG_T Cuts at: 6924 6924 Size: 7048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2013 3517 6162 6346 1305 Size: 708 1504 2645 184 2007 Fragments arranged by size: 2645 2007 1504 708 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 2693 4179 4227 10 Size: 54 1425 343 210 651 1486 48 2831 Fragments arranged by size: 2831 1486 1425 651 343 210 54 48 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2115 2676 4167 4236 5006 5365 Size: 648 876 73 561 1491 69 770 359 Cuts at: 5365 5524 518 Size: 159 2042 Fragments arranged by size: 2042 1491 876 770 648 561 359 159 73 69 MspA1I CmG'CkG Cuts at: 1606 2648 3266 3808 3971 4324 4464 5141 6289 Size: 1042 618 542 163 353 140 677 1148 Cuts at: 6289 6534 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1042 677 618 542 353 245 163 140 NaeI GCC'GGC Cuts at: 3069 3069 Size: 7048 NcoI C'CATG_G Cuts at: 513 1065 2004 2043 3660 513 Size: 552 939 39 1617 3901 Fragments arranged by size: 3901 1617 939 552 39 NdeI CA'TA_TG Cuts at: 387 3964 387 Size: 3577 3471 NgoMIV G'CCGG_C Cuts at: 3067 3067 Size: 7048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7048 NotI GC'GGCC_GC Cuts at: 4495 4495 Size: 7048 NspI r_CATG'y Cuts at: 1132 1229 2326 2408 4280 4406 5951 1132 Size: 97 1097 82 1872 126 1545 2229 Fragments arranged by size: 2229 1872 1545 1097 126 97 82 PasI CC'CwG_GG Cuts at: 1348 4111 1348 Size: 2763 4285 PciI A'CATG_T Cuts at: 5947 5947 Size: 7048 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2503 4256 1108 Size: 1269 126 1753 3900 Fragments arranged by size: 3900 1753 1269 126 PmeI GTTT'AAAC Cuts at: 4352 4352 Size: 7048 PmlI CAC'GTG Cuts at: 2456 2456 Size: 7048 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5117 5149 6630 6662 5117 Size: 32 1481 32 5503 Fragments arranged by size: 5503 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2652 2991 3853 4285 2652 Size: 339 862 432 5415 Fragments arranged by size: 5415 862 432 339 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7048 PsiI TTA'TAA Cuts at: 3300 4618 3300 Size: 1318 5730 PspXI vC'TCGA_Gb Cuts at: 2271 2271 Size: 7048 PstI C_TGCA'G Cuts at: 830 2521 2844 3850 3889 4400 830 Size: 1691 323 1006 39 511 3478 Fragments arranged by size: 3478 1691 1006 511 323 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7048 PvuII CAG'CTG Cuts at: 3266 3808 3971 4324 3266 Size: 542 163 353 5990 Fragments arranged by size: 5990 542 353 163 SacI G_AGCT'C Cuts at: 2278 2278 Size: 7048 SalI G'TCGA_C Cuts at: 1936 4390 1936 Size: 2454 4594 SapI GCTCTTCn'nnn_ Cuts at: 3411 3411 Size: 7048 SbfI CC_TGCA'GG Cuts at: 4400 4400 Size: 7048 ScaI AGT'ACT Cuts at: 5296 7008 5296 Size: 1712 5336 SexAI A'CCwGG_T Cuts at: 2496 4344 2496 Size: 1848 5200 SfcI C'TryA_G Cuts at: 826 1045 2517 2840 3846 3885 4396 5531 6212 Size: 219 1472 323 1006 39 511 1135 681 Cuts at: 6212 6403 826 Size: 191 1471 Fragments arranged by size: 1472 1471 1135 1006 681 511 323 219 191 39 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7048 SmaI CCC'GGG Cuts at: 1872 4375 1872 Size: 2503 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2013 2229 2271 5152 6053 6315 Size: 197 926 70 216 42 2881 901 262 Cuts at: 6315 820 Size: 1553 Fragments arranged by size: 2881 1553 926 901 262 216 197 70 42 SnaBI TAC'GTA Cuts at: 493 2603 4839 493 Size: 2110 2236 2702 Fragments arranged by size: 2702 2236 2110 SpeI A'CTAG_T Cuts at: 152 3610 152 Size: 3458 3590 SphI G_CATG'C Cuts at: 1132 2326 4280 4406 1132 Size: 1194 1954 126 3774 Fragments arranged by size: 3774 1954 1194 126 SspI AAT'ATT Cuts at: 5 52 4972 5 Size: 47 4920 2081 Fragments arranged by size: 4920 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7048 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 3208 3660 4123 4188 4487 Size: 552 939 39 1165 452 463 65 299 Cuts at: 4487 513 Size: 3074 Fragments arranged by size: 3074 1165 939 552 463 452 299 65 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3317 3516 3653 4211 5075 5092 5245 Size: 1255 1737 199 137 558 864 17 153 Cuts at: 5245 5430 5875 325 Size: 185 445 1498 Fragments arranged by size: 1737 1498 1255 864 558 445 199 185 153 137 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5294 7006 96 Size: 274 80 33 51 1443 3317 1712 138 Fragments arranged by size: 3317 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2445 2477 3491 3523 3937 3969 7008 7040 2445 Size: 32 1014 32 414 32 3039 32 2453 Fragments arranged by size: 3039 2453 1014 414 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7048 XbaI T'CTAG_A Cuts at: 4384 4384 Size: 7048 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3510 3510 Size: 7048 XhoI C'TCGA_G Cuts at: 1943 2271 1943 Size: 328 6720 XmaI C'CCGG_G Cuts at: 1870 4373 1870 Size: 2503 4545 XmnI GAAnn'nnTTC Cuts at: 2110 3274 5177 2110 Size: 1164 1903 3981 Fragments arranged by size: 3981 1903 1164 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4856 276 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI