Restriction map for pFN21AE3123
 (Circular) (Six-base) MAPSORT of: pFN21AE3123.seq  Check: 7322  from: 1  to: 5635



pFN21AE3123  5635 bp

 With 182 enzymes: SgfI * 

                        October 25, 2019 12:32  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3445     278 
   Size:         53      83     186     936    1909    2468

  Fragments arranged by size: 

                2468    1909     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2978    1937 
   Size:       1041    4594
 
Acc65I G'GTAC_C

Cuts at:   2956    2956 
   Size:       5635
 
AclI AA'CG_TT

Cuts at:   1563    3762    4135    1563 
   Size:       2199     373    3063

  Fragments arranged by size: 

                3063    2199     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2467    2681    3698    5082    5350    1632 
   Size:        835     214    1017    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1017     835     268     214
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5438
 
AflIII A'CryG_T

Cuts at:   1203    2863    4534    5511    1203 
   Size:       1660    1671     977    1327

  Fragments arranged by size: 

                1671    1660    1327     977
 
AgeI A'CCGG_T

Cuts at:   4466    4466 
   Size:       5635
 
AhdI GACnn_n'nnGTC

Cuts at:   4364    4364 
   Size:       5635
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5635
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5635
 
AlwNI CAG_nnn'CTG

Cuts at:   3378    4950    3378 
   Size:       1572    4063
 
ApaLI G'TGCA_C

Cuts at:   3692    4848    3692 
   Size:       1156    4479
 
ApoI r'AATT_y

Cuts at:   1493    2183    2659    2944    3170    3322    5431    1493 
   Size:        690     476     285     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     690     476     285     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2579    4189     160 
   Size:        872    1547    1610    1606

  Fragments arranged by size: 

                1610    1606    1547     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5635
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2960    1870 
   Size:         73    1017    4545

  Fragments arranged by size: 

                4545    1017      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2910    2943    2910 
   Size:         33    5602
 
BamHI G'GATC_C

Cuts at:   2440    2965    3332    2440 
   Size:        525     367    4743

  Fragments arranged by size: 

                4743     525     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2956    4411     618 
   Size:        325     217     627    1169    1455    1842

  Fragments arranged by size: 

                1842    1455    1169     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2954    1391 
   Size:       1563    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4664
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3826    3860    1903 
   Size:         34    1889      34    3678

  Fragments arranged by size: 

                3678    1889      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2911    3527    4737    2911 
   Size:        616    1210    3809

  Fragments arranged by size: 

                3809    1210     616
 
BclI T'GATC_A

Cuts at:   1548    2490    1548 
   Size:        942    4693
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4246     136 
   Size:        107     122      71     631     605    2574    1525

  Fragments arranged by size: 

                2574    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5630    5630 
   Size:       5635
 
BlpI GC'TnA_GC

Cuts at:   3052    3052 
   Size:       5635
 
Bme1580I G_kGCm'C

Cuts at:   1790    3696    4852    1790 
   Size:       1906    1156    2573

  Fragments arranged by size: 

                2573    1906    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5635
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2054    3366    4324 
   Size:        416      53      83     399      24     637    1312     958

Cuts at:   4324     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1312     958     637     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5635
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2166    4295    1239 
   Size:        927    2129    2579

  Fragments arranged by size: 

                2579    2129     927
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3760    4625    4923    3760 
   Size:        865     298    4472

  Fragments arranged by size: 

                4472     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4298     882 
   Size:        617    2799    2219

  Fragments arranged by size: 

                2799    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3426     493 
   Size:        711    2222    2702

  Fragments arranged by size: 

                2702    2222     711
 
BsaBI GATnn'nnATC

Cuts at:   5629    5629 
   Size:       5635
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3442    3824    4459     275 
   Size:         53      83     186     936    1909     382     635    1451

  Fragments arranged by size: 

                1909    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4068    4466    4740    4887    5298    4068 
   Size:        398     274     147     411    4405

  Fragments arranged by size: 

                4405     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2053    2083    2172    2202    1178 
   Size:         30     165      30     650      30      89      30    4611

  Fragments arranged by size: 

                4611     650     165      89      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2043    1186 
   Size:        857    4778
 
BseYI C'CCAG_C

Cuts at:   4838    5334    4838 
   Size:        496    5139
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5062
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3085    3846    4874    1540 
   Size:        275     186    1084     761    1028    2301

  Fragments arranged by size: 

                2301    1084    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3696    3781    4852    3696 
   Size:         85    1071    4479

  Fragments arranged by size: 

                4479    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3146    3239    3146 
   Size:         93    5542
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2954    3696    3781    4852    1391 
   Size:        399    1164     742      85    1071    2174

  Fragments arranged by size: 

                2174    1164    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5298    5298 
   Size:       5635
 
BspHI T'CATG_A

Cuts at:   3524    5254    3524 
   Size:       1730    3905
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2990     844 
   Size:       2146    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3522    1394 
   Size:        429    1699    3507

  Fragments arranged by size: 

                3507    1699     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4130    4304      65 
   Size:       1183    2882     174    1396

  Fragments arranged by size: 

                2882    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4279    4466    1700 
   Size:        110    2469     187    2869

  Fragments arranged by size: 

                2869    2469     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5635
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5635
 
BssSI C'ACGA_G

Cuts at:   3695    4707    3695 
   Size:       1012    4623
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5635
 
BstBI TT'CG_AA

Cuts at:   2540    4439    2540 
   Size:       1899    3736
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5635
 
BstYI r'GATC_y

Cuts at:   1972    2440    2965    3332    3717    3734    5186    5272    5284 
   Size:        468     525     367     385      17    1452      86      12

Cuts at:   5284    5630    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     525     468     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4144

  Fragments arranged by size: 

                4144     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4896

  Fragments arranged by size: 

                4896     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2082    2436    3151    3945    3965    2082 
   Size:        354     715     794      20    3752

  Fragments arranged by size: 

                3752     794     715     354      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5635
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5600
 
DraI TTT'AAA

Cuts at:   2932    2939    2932 
   Size:          7    5628
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4642     809 
   Size:        507    3326    1802

  Fragments arranged by size: 

                3326    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2137    3082    3971 
   Size:         54    1425      50     275      18     307     945     889

Cuts at:   3971    5600       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     945     889     307     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3082    1537 
   Size:        275    1270    4090

  Fragments arranged by size: 

                4090    1270     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2110    2666    3565    1353 
   Size:        757     556     899    3423

  Fragments arranged by size: 

                3423     899     757     556
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4222    4596    4742     387 
   Size:       1064     138    2633     374     146    1280

  Fragments arranged by size: 

                2633    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2166    2467    2681    3698    4295    5082    5350 
   Size:        393     534     301     214    1017     597     787     268

Cuts at:   5350    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1017     787     597     534     393     301     268
                 214
 
EcoO109I rG'GnC_Cy

Cuts at:   2839    2839 
   Size:       5635
 
EcoRI G'AATT_C

Cuts at:   2944    3322    2944 
   Size:        378    5257
 
EcoRV GAT'ATC

Cuts at:   2383    2383 
   Size:       5635
 
FspI TGC'GCA

Cuts at:   4141    4141 
   Size:       5635
 
HaeII r_GCGC'y

Cuts at:   1678    4782    1678 
   Size:       3104    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4276    4308    4350    4382    1373 
   Size:         32     135      32    2704      32      42      32    2626

  Fragments arranged by size: 

                2704    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2979    3225     669 
   Size:        988     281    1041     246    3079

  Fragments arranged by size: 

                3079    1041     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5635
 
HpaI GTT'AAC

Cuts at:   3225    3225 
   Size:       5635
 
KpnI G_GTAC'C

Cuts at:   2960    2960 
   Size:       5635
 
MfeI C'AATT_G

Cuts at:   3234    3234 
   Size:       5635
 
MluI A'CGCG_T

Cuts at:   5511    5511 
   Size:       5635
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2271    2394    2657    2730    2895    4749    4933    1305 
   Size:        966     123     263      73     165    1854     184    2007

  Fragments arranged by size: 

                2007    1854     966     263     184     165     123      73
            
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3813

  Fragments arranged by size: 

                3813    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2139    2868    3593    3952    4111     518 
   Size:        648     973     729     725     359     159    2042

  Fragments arranged by size: 

                2042     973     729     725     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2812    3051    3728    4876    5121    1606 
   Size:       1206     239     677    1148     245    2120

  Fragments arranged by size: 

                2120    1206    1148     677     245     239
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4144

  Fragments arranged by size: 

                4144     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5635
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5635
 
NotI GC'GGCC_GC

Cuts at:   3082    3082 
   Size:       5635
 
NspI r_CATG'y

Cuts at:   1132    1229    2867    2993    4538    1132 
   Size:         97    1638     126    1545    2229

  Fragments arranged by size: 

                2229    1638    1545     126      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5635
 
PciI A'CATG_T

Cuts at:   2863    4534    2863 
   Size:       1671    3964
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5635
 
PmeI GTTT'AAAC

Cuts at:   2939    2939 
   Size:       5635
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3704    3736    5217    5249    3704 
   Size:         32    1481      32    4090

  Fragments arranged by size: 

                4090    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2839    2839 
   Size:       5635
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5635
 
PsiI TTA'TAA

Cuts at:   3205    3205 
   Size:       5635
 
PstI C_TGCA'G

Cuts at:    830    2077    2987     830 
   Size:       1247     910    3478

  Fragments arranged by size: 

                3478    1247     910
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5635
 
PvuII CAG'CTG

Cuts at:   2812    2812 
   Size:       5635
 
SalI G'TCGA_C

Cuts at:   1936    2977    1936 
   Size:       1041    4594
 
SbfI CC_TGCA'GG

Cuts at:   2987    2987 
   Size:       5635
 
ScaI AGT'ACT

Cuts at:   3883    5595    3883 
   Size:       1712    3923
 
SfcI C'TryA_G

Cuts at:    826    1045    2061    2073    2704    2778    2983    4118    4799 
   Size:        219    1016      12     631      74     205    1135     681

Cuts at:   4799    4990     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1135    1016     681     631     219     205     191
                  74      12
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5635
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5635
 
SmaI CCC'GGG

Cuts at:   1872    2962    1872 
   Size:       1090    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3739    4640    4902     820 
   Size:        197     926    1796     901     262    1553

  Fragments arranged by size: 

                1796    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3426     493 
   Size:       2933    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5635
 
SphI G_CATG'C

Cuts at:   1132    2993    1132 
   Size:       1861    3774
 
SspI AAT'ATT

Cuts at:      5      52    2890    3559       5 
   Size:         47    2838     669    2081

  Fragments arranged by size: 

                2838    2081     669      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5635
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3074     513 
   Size:        552     939    1070    3074

  Fragments arranged by size: 

                3074    1070     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3662    3679    3832    4017    4462     325 
   Size:       1255    2082      17     153     185     445    1498

  Fragments arranged by size: 

                2082    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2872    3881    5593 
   Size:        274      80      33      51    1443     895    1009    1712

Cuts at:   5593      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1009     895     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5595    5627    5595 
   Size:         32    5603
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5635
 
XbaI T'CTAG_A

Cuts at:   2971    2971 
   Size:       5635
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2851    2851 
   Size:       5635
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5635
 
XmaI C'CCGG_G

Cuts at:   1870    2960    1870 
   Size:       1090    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3764    3764 
   Size:       5635
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3443     276 
   Size:         53      83     186     936    1909    2468

  Fragments arranged by size: 

                2468    1909     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI
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