(Circular) (Six-base) MAPSORT of: pFN21AE3661.seq Check: 6353 from: 1 to: 5968 pFN21AE3661 5968 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3105 3311 1937 Size: 1168 206 4594 Fragments arranged by size: 4594 1168 206 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2311 2822 2852 3102 4031 5415 5683 1632 Size: 679 511 30 250 929 1384 268 1917 Fragments arranged by size: 1917 1384 929 679 511 268 250 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 4867 5844 1203 Size: 3664 977 1327 Fragments arranged by size: 3664 1327 977 AgeI A'CCGG_T Cuts at: 4799 4799 Size: 5968 AhdI GACnn_n'nnGTC Cuts at: 4697 4697 Size: 5968 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5968 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5968 AlwNI CAG_nnn'CTG Cuts at: 2560 3711 5283 2560 Size: 1151 1572 3245 Fragments arranged by size: 3245 1572 1151 ApaI G_GGCC'C Cuts at: 3035 3035 Size: 5968 ApaLI G'TGCA_C Cuts at: 2642 4025 5181 2642 Size: 1383 1156 3429 Fragments arranged by size: 3429 1383 1156 ApoI r'AATT_y Cuts at: 1493 3277 3503 3655 5764 1493 Size: 1784 226 152 2109 1697 Fragments arranged by size: 2109 1784 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4522 160 Size: 872 3490 1606 Fragments arranged by size: 3490 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 2679 3293 1870 Size: 73 736 614 4545 Fragments arranged by size: 4545 736 614 73 BamHI G'GATC_C Cuts at: 3298 3665 3298 Size: 367 5601 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2197 2981 3289 4744 618 Size: 325 217 627 410 784 308 1455 1842 Fragments arranged by size: 1842 1455 784 627 410 325 308 217 BanII G_rGCy'C Cuts at: 1391 2190 2885 2975 3035 3091 3287 1391 Size: 799 695 90 60 56 196 4072 Fragments arranged by size: 4072 799 695 196 90 60 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2317 2598 2627 928 Size: 971 418 281 29 4269 Fragments arranged by size: 4269 971 418 281 29 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2476 3860 5070 2476 Size: 1384 1210 3374 Fragments arranged by size: 3374 1384 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BfrBI ATG'CAT Cuts at: 2491 2491 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4579 136 Size: 107 122 71 631 605 2907 1525 Fragments arranged by size: 2907 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3201 5963 3201 Size: 2762 3206 BlpI GC'TnA_GC Cuts at: 2189 3231 3385 2189 Size: 1042 154 4772 Fragments arranged by size: 4772 1042 154 Bme1580I G_kGCm'C Cuts at: 1790 2646 3035 4029 5185 1790 Size: 856 389 994 1156 2573 Fragments arranged by size: 2573 1156 994 856 389 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2283 2548 3699 Size: 416 53 83 399 24 866 265 1151 Cuts at: 3699 4657 442 Size: 958 1753 Fragments arranged by size: 1753 1151 958 866 416 399 265 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2813 4628 1239 Size: 1574 1815 2579 Fragments arranged by size: 2579 1815 1574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2645 4093 4958 5256 2645 Size: 1448 865 298 3357 Fragments arranged by size: 3357 1448 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4631 882 Size: 617 3132 2219 Fragments arranged by size: 3132 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3759 493 Size: 711 2555 2702 Fragments arranged by size: 2702 2555 711 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3775 4157 4792 Size: 53 83 186 936 476 1766 382 635 Cuts at: 4792 275 Size: 1451 Fragments arranged by size: 1766 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3072 3217 4401 4799 5073 5220 5631 3072 Size: 145 1184 398 274 147 411 3409 Fragments arranged by size: 3409 1184 411 398 274 147 145 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2906 2936 2941 2971 1178 Size: 30 165 30 1503 30 5 30 4175 Fragments arranged by size: 4175 1503 165 30 30 30 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2005 2789 2909 1186 Size: 819 784 120 4245 Fragments arranged by size: 4245 819 784 120 BseYI C'CCAG_C Cuts at: 2396 3191 5171 5667 2396 Size: 795 1980 496 2697 Fragments arranged by size: 2697 1980 795 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 2846 1163 Size: 573 390 720 4285 Fragments arranged by size: 4285 720 573 390 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2136 2443 3418 4179 5207 1540 Size: 275 186 135 307 975 761 1028 2301 Fragments arranged by size: 2301 1028 975 761 307 275 186 135 BsiHKAI G_wGCw'C Cuts at: 2190 2646 4029 4114 5185 2190 Size: 456 1383 85 1071 2973 Fragments arranged by size: 2973 1383 1071 456 85 BsmI GAATG_Cn' Cuts at: 3479 3572 3479 Size: 93 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2646 2885 2975 3035 3091 3287 Size: 399 400 456 239 90 60 56 196 Cuts at: 3287 4029 4114 5185 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 456 400 399 239 196 90 85 60 56 BspEI T'CCGG_A Cuts at: 3072 3217 5631 3072 Size: 145 2414 3409 Fragments arranged by size: 3409 2414 145 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3323 844 Size: 2479 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2478 4463 4637 65 Size: 1183 1230 1985 174 1396 Fragments arranged by size: 1985 1396 1230 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4612 4799 1700 Size: 110 2802 187 2869 Fragments arranged by size: 2869 2802 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5968 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 4028 5040 4028 Size: 1012 4956 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 3164 794 Size: 1310 1060 3598 Fragments arranged by size: 3598 1310 1060 BstBI TT'CG_AA Cuts at: 4772 4772 Size: 5968 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5968 BstYI r'GATC_y Cuts at: 1972 3201 3298 3665 4050 4067 5519 5605 5617 Size: 1229 97 367 385 17 1452 86 12 Cuts at: 5617 5963 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1229 385 367 346 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2723 2723 Size: 5968 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2533 513 Size: 552 939 27 502 3948 Fragments arranged by size: 3948 939 552 502 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5229 Fragments arranged by size: 5229 683 56 BtsI GCAGTG_nn' Cuts at: 2419 3484 4278 4298 2419 Size: 1065 794 20 4089 Fragments arranged by size: 4089 1065 794 20 ClaI AT'CG_AT Cuts at: 1554 2344 1554 Size: 790 5178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5933 DraI TTT'AAA Cuts at: 3272 3272 Size: 5968 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 3415 4304 Size: 54 1425 50 275 18 303 1282 889 Cuts at: 4304 5933 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1282 889 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2133 3415 1537 Size: 275 321 1282 4090 Fragments arranged by size: 4090 1282 321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2766 2798 3898 1353 Size: 1413 32 1100 3423 Fragments arranged by size: 3423 1413 1100 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4555 4929 5075 387 Size: 1064 138 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2188 2188 Size: 5968 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2311 2813 2822 2852 3102 4031 4628 Size: 393 679 502 9 30 250 929 597 Cuts at: 4628 5415 5683 1239 Size: 787 268 1524 Fragments arranged by size: 1524 929 787 679 597 502 393 268 250 30 9 EcoO109I rG'GnC_Cy Cuts at: 2054 2054 Size: 5968 EcoRI G'AATT_C Cuts at: 3277 3655 3277 Size: 378 5590 EcoRV GAT'ATC Cuts at: 3127 3127 Size: 5968 FspI TGC'GCA Cuts at: 2615 4474 2615 Size: 1859 4109 HaeII r_GCGC'y Cuts at: 1678 2906 5115 1678 Size: 1228 2209 2531 Fragments arranged by size: 2531 2209 1228 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2906 2938 2941 2973 2994 Size: 32 135 32 1334 32 3 32 21 Cuts at: 2994 3026 3032 3064 4609 4641 4683 4715 1373 Size: 32 6 32 1545 32 42 32 2626 Fragments arranged by size: 2626 1545 1334 135 42 32 32 32 32 32 32 32 32 21 6 3 HincII GTy'rAC Cuts at: 669 1657 1938 2257 3312 3558 669 Size: 988 281 319 1055 246 3079 Fragments arranged by size: 3079 1055 988 319 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2529 5082 5266 1305 Size: 1224 2553 184 2007 Fragments arranged by size: 2553 2007 1224 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4146 Fragments arranged by size: 4146 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2606 3926 4285 4444 518 Size: 648 1440 1320 359 159 2042 Fragments arranged by size: 2042 1440 1320 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2830 3384 4061 5209 5454 1606 Size: 537 687 554 677 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 554 537 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2533 513 Size: 552 939 529 3948 Fragments arranged by size: 3948 939 552 529 NdeI CA'TA_TG Cuts at: 387 387 Size: 5968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NsiI A_TGCA'T Cuts at: 2493 2493 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 2491 2605 3326 4871 1132 Size: 97 1262 114 721 1545 2229 Fragments arranged by size: 2229 1545 1262 721 114 97 PasI CC'CwG_GG Cuts at: 1348 3028 1348 Size: 1680 4288 PciI A'CATG_T Cuts at: 4867 4867 Size: 5968 PflMI CCAn_nnn'nTGG Cuts at: 1108 2560 1108 Size: 1452 4516 PfoI T'CCnGG_A Cuts at: 3240 3240 Size: 5968 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2450 1613 Size: 837 5131 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PspOMI G'GGCC_C Cuts at: 3031 3031 Size: 5968 PstI C_TGCA'G Cuts at: 830 2945 3320 830 Size: 2115 375 3478 Fragments arranged by size: 3478 2115 375 PvuI CG_AT'CG Cuts at: 2001 2443 2001 Size: 442 5526 PvuII CAG'CTG Cuts at: 2830 2830 Size: 5968 SacI G_AGCT'C Cuts at: 2190 2190 Size: 5968 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 4216 5928 4216 Size: 1712 4256 SfcI C'TryA_G Cuts at: 826 1045 2845 2941 3316 4451 5132 5323 826 Size: 219 1800 96 375 1135 681 191 1471 Fragments arranged by size: 1800 1471 1135 681 375 219 191 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 2681 3295 1872 Size: 809 614 4545 Fragments arranged by size: 4545 809 614 SmlI C'TyrA_G Cuts at: 820 1017 1943 2660 4072 4973 5235 820 Size: 197 926 717 1412 901 262 1553 Fragments arranged by size: 1553 1412 926 901 717 262 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 3326 1132 Size: 2194 3774 SspI AAT'ATT Cuts at: 5 52 2787 3892 5 Size: 47 2735 1105 2081 Fragments arranged by size: 2735 2081 1105 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5968 StyI C'CwwG_G Cuts at: 513 1065 2004 2533 3407 513 Size: 552 939 529 874 3074 Fragments arranged by size: 3074 939 874 552 529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2008 2138 3995 4012 4165 4350 4795 Size: 1255 428 130 1857 17 153 185 445 Cuts at: 4795 325 Size: 1498 Fragments arranged by size: 1857 1498 1255 445 428 185 153 130 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2517 4214 5926 Size: 274 80 33 51 1443 540 1697 1712 Cuts at: 5926 96 Size: 138 Fragments arranged by size: 1712 1697 1443 540 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2323 2355 5928 5960 2323 Size: 32 3573 32 2331 Fragments arranged by size: 3573 2331 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5968 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5968 XmaI C'CCGG_G Cuts at: 1870 2679 3293 1870 Size: 809 614 4545 Fragments arranged by size: 4545 809 614 XmnI GAAnn'nnTTC Cuts at: 2099 2123 4097 2099 Size: 24 1974 3970 Fragments arranged by size: 3970 1974 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI