Restriction map for pFN21AE3661
 (Linear) (Six-base) MAPSORT of: KIEE3661.seq  Check: 8200  from: 1  to: 1263



KIEE3661.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

AccI GT'mk_AC

Cuts at:      0    1100    1263 
   Size:       1100     163
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     817     847    1097    1263 
   Size:        306     511      30     250     166

  Fragments arranged by size: 

                 511     306     250     166      30
 
AlwNI CAG_nnn'CTG

Cuts at:      0     555    1263 
   Size:        555     708
 
ApaI G_GGCC'C

Cuts at:      0    1030    1263 
   Size:       1030     233
 
ApaLI G'TGCA_C

Cuts at:      0     637    1263 
   Size:        637     626
 
AvaI C'yCGr_G

Cuts at:      0     674    1263 
   Size:        674     589
 
BanI G'GyrC_C

Cuts at:      0     192     976    1263 
   Size:        192     784     287

  Fragments arranged by size: 

                 784     287     192
 
BanII G_rGCy'C

Cuts at:      0     185     880     970    1030    1086    1263 
   Size:        185     695      90      60      56     177

  Fragments arranged by size: 

                 695     185     177      90      60      56
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     312     593     622    1263 
   Size:        312     281      29     641

  Fragments arranged by size: 

                 641     312     281      29
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     471    1263 
   Size:        471     792
 
BfrBI ATG'CAT

Cuts at:      0     486    1263 
   Size:        486     777
 
BglII A'GATC_T

Cuts at:      0    1196    1263 
   Size:       1196      67
 
BlpI GC'TnA_GC

Cuts at:      0     184    1226    1263 
   Size:        184    1042      37

  Fragments arranged by size: 

                1042     184      37
 
Bme1580I G_kGCm'C

Cuts at:      0     641    1030    1263 
   Size:        641     389     233

  Fragments arranged by size: 

                 641     389     233
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     278     543    1263 
   Size:        278     265     720

  Fragments arranged by size: 

                 720     278     265
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     808    1263 
   Size:        808     455
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640    1263 
   Size:        640     623
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1263 
   Size:          4    1259
 
BsaWI w'CCGG_w

Cuts at:      0    1067    1212    1263 
   Size:       1067     145      51

  Fragments arranged by size: 

                1067     145      51
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     901     931     936     966    1263 
   Size:        901      30       5      30     297

  Fragments arranged by size: 

                 901     297      30      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     784     904    1263 
   Size:        784     120     359

  Fragments arranged by size: 

                 784     359     120
 
BseYI C'CCAG_C

Cuts at:      0     391    1186    1263 
   Size:        391     795      77

  Fragments arranged by size: 

                 795     391      77
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     841    1263 
   Size:        121     720     422

  Fragments arranged by size: 

                 720     422     121
 
BsiEI CG_ry'CG

Cuts at:      0     131     438    1263 
   Size:        131     307     825

  Fragments arranged by size: 

                 825     307     131
 
BsiHKAI G_wGCw'C

Cuts at:      0     185     641    1263 
   Size:        185     456     622

  Fragments arranged by size: 

                 622     456     185
 
Bsp1286I G_dGCh'C

Cuts at:      0     185     641     880     970    1030    1086    1263 
   Size:        185     456     239      90      60      56     177

  Fragments arranged by size: 

                 456     239     185     177      90      60      56
 
BspEI T'CCGG_A

Cuts at:      0    1067    1212    1263 
   Size:       1067     145      51

  Fragments arranged by size: 

                1067     145      51
 
BsrDI GCAATG_nn'

Cuts at:      0     473    1263 
   Size:        473     790
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99    1159    1263 
   Size:         99    1060     104

  Fragments arranged by size: 

                1060     104      99
 
BstYI r'GATC_y

Cuts at:      0    1196    1263 
   Size:       1196      67
 
Bsu36I CC'TnA_GG

Cuts at:      0     718    1263 
   Size:        718     545
 
BtgI C'CryG_G

Cuts at:      0      26     528    1263 
   Size:         26     502     735

  Fragments arranged by size: 

                 735     502      26
 
BtsI GCAGTG_nn'

Cuts at:      0     414    1263 
   Size:        414     849
 
ClaI AT'CG_AT

Cuts at:      0     339    1263 
   Size:        339     924
 
EaeI y'GGCC_r

Cuts at:      0     128    1263 
   Size:        128    1135
 
EagI C'GGCC_G

Cuts at:      0     128    1263 
   Size:        128    1135
 
EarI CTCTTCn'nnn_

Cuts at:      0     761     793    1263 
   Size:        761      32     470

  Fragments arranged by size: 

                 761     470      32
 
EcoICRI GAG'CTC

Cuts at:      0     183    1263 
   Size:        183    1080
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     808     817     847    1097    1263 
   Size:        306     502       9      30     250     166

  Fragments arranged by size: 

                 502     306     250     166      30       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      49    1263 
   Size:         49    1214
 
EcoRV GAT'ATC

Cuts at:      0    1122    1263 
   Size:       1122     141
 
FspI TGC'GCA

Cuts at:      0     610    1263 
   Size:        610     653
 
HaeII r_GCGC'y

Cuts at:      0     901    1263 
   Size:        901     362
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     901     933     936     968     989    1021    1027    1059 
   Size:        901      32       3      32      21      32       6      32

Cuts at:   1059    1263 
   Size:        204

  Fragments arranged by size: 

                 901     204      32      32      32      32      21       6
                   3
 
HincII GTy'rAC

Cuts at:      0     252    1263 
   Size:        252    1011
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     524    1263 
   Size:        524     739
 
MslI CAynn'nnrTG

Cuts at:      0     601    1263 
   Size:        601     662
 
MspA1I CmG'CkG

Cuts at:      0     138     825    1263 
   Size:        138     687     438

  Fragments arranged by size: 

                 687     438     138
 
NcoI C'CATG_G

Cuts at:      0     528    1263 
   Size:        528     735
 
NsiI A_TGCA'T

Cuts at:      0     488    1263 
   Size:        488     775
 
NspI r_CATG'y

Cuts at:      0     486     600    1263 
   Size:        486     114     663

  Fragments arranged by size: 

                 663     486     114
 
PasI CC'CwG_GG

Cuts at:      0    1023    1263 
   Size:       1023     240
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     555    1263 
   Size:        555     708
 
PfoI T'CCnGG_A

Cuts at:      0    1235    1263 
   Size:       1235      28
 
PshAI GACnn'nnGTC

Cuts at:      0     445    1263 
   Size:        445     818
 
PspOMI G'GGCC_C

Cuts at:      0    1026    1263 
   Size:       1026     237
 
PstI C_TGCA'G

Cuts at:      0     940    1263 
   Size:        940     323
 
PvuI CG_AT'CG

Cuts at:      0     438    1263 
   Size:        438     825
 
PvuII CAG'CTG

Cuts at:      0     825    1263 
   Size:        825     438
 
SacI G_AGCT'C

Cuts at:      0     185    1263 
   Size:        185    1078
 
SfcI C'TryA_G

Cuts at:      0     840     936    1263 
   Size:        840      96     327

  Fragments arranged by size: 

                 840     327      96
 
SmaI CCC'GGG

Cuts at:      0     676    1263 
   Size:        676     587
 
SmlI C'TyrA_G

Cuts at:      0     655    1263 
   Size:        655     608
 
SspI AAT'ATT

Cuts at:      0     782    1263 
   Size:        782     481
 
StyI C'CwwG_G

Cuts at:      0     528    1263 
   Size:        528     735
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       3     133    1263 
   Size:          3     130    1130

  Fragments arranged by size: 

                1130     130       3
 
TatI w'GTAC_w

Cuts at:      0     512    1263 
   Size:        512     751
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     318     350    1263 
   Size:        318      32     913

  Fragments arranged by size: 

                 913     318      32
 
XmaI C'CCGG_G

Cuts at:      0     674    1263 
   Size:        674     589
 
XmnI GAAnn'nnTTC

Cuts at:      0      94     118    1263 
   Size:         94      24    1145

  Fragments arranged by size: 

                1145      94      24
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII
     BbsI    BciVI    BfrBI    BglII     BlpI Bme1580I     BmrI     BpmI
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BsrDI   BstAPI    BstYI   Bsu36I     BtgI
     BtsI     ClaI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FspI    HaeII    Hin4I   HincII     MmeI     MslI   MspA1I
     NcoI     NsiI     NspI     PasI    PflMI     PfoI    PshAI   PspOMI
     PstI     PvuI    PvuII     SacI     SfcI     SmaI     SmlI     SspI
     StyI    TaqII     TatI     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI     BglI
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsiWI
     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I    BtgZI    CspCI     DraI
   DraIII     DrdI     EciI    EcoNI    EcoRI     FalI     FseI    FspAI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI
     PmeI     PmlI     PpiI    PpuMI     PsiI    PspXI     PsrI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI  Tth111I     XbaI     XcmI     XhoI     ZraI
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