(Linear) (Six-base) MAPSORT of: KIEE3661.seq Check: 8200 from: 1 to: 1263 KIEE3661.orf With 182 enzymes: SgfI * December 27, 2011 16:37 .. AccI GT'mk_AC Cuts at: 0 1100 1263 Size: 1100 163 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 817 847 1097 1263 Size: 306 511 30 250 166 Fragments arranged by size: 511 306 250 166 30 AlwNI CAG_nnn'CTG Cuts at: 0 555 1263 Size: 555 708 ApaI G_GGCC'C Cuts at: 0 1030 1263 Size: 1030 233 ApaLI G'TGCA_C Cuts at: 0 637 1263 Size: 637 626 AvaI C'yCGr_G Cuts at: 0 674 1263 Size: 674 589 BanI G'GyrC_C Cuts at: 0 192 976 1263 Size: 192 784 287 Fragments arranged by size: 784 287 192 BanII G_rGCy'C Cuts at: 0 185 880 970 1030 1086 1263 Size: 185 695 90 60 56 177 Fragments arranged by size: 695 185 177 90 60 56 BbsI GAAGACnn'nnnn_ Cuts at: 0 312 593 622 1263 Size: 312 281 29 641 Fragments arranged by size: 641 312 281 29 BciVI GTATCCnnnnn_n' Cuts at: 0 471 1263 Size: 471 792 BfrBI ATG'CAT Cuts at: 0 486 1263 Size: 486 777 BglII A'GATC_T Cuts at: 0 1196 1263 Size: 1196 67 BlpI GC'TnA_GC Cuts at: 0 184 1226 1263 Size: 184 1042 37 Fragments arranged by size: 1042 184 37 Bme1580I G_kGCm'C Cuts at: 0 641 1030 1263 Size: 641 389 233 Fragments arranged by size: 641 389 233 BmrI ACTGGGnnnn_n' Cuts at: 0 278 543 1263 Size: 278 265 720 Fragments arranged by size: 720 278 265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 808 1263 Size: 808 455 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1263 Size: 640 623 BsaHI Gr'CG_yC Cuts at: 0 4 1263 Size: 4 1259 BsaWI w'CCGG_w Cuts at: 0 1067 1212 1263 Size: 1067 145 51 Fragments arranged by size: 1067 145 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 901 931 936 966 1263 Size: 901 30 5 30 297 Fragments arranged by size: 901 297 30 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 784 904 1263 Size: 784 120 359 Fragments arranged by size: 784 359 120 BseYI C'CCAG_C Cuts at: 0 391 1186 1263 Size: 391 795 77 Fragments arranged by size: 795 391 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 841 1263 Size: 121 720 422 Fragments arranged by size: 720 422 121 BsiEI CG_ry'CG Cuts at: 0 131 438 1263 Size: 131 307 825 Fragments arranged by size: 825 307 131 BsiHKAI G_wGCw'C Cuts at: 0 185 641 1263 Size: 185 456 622 Fragments arranged by size: 622 456 185 Bsp1286I G_dGCh'C Cuts at: 0 185 641 880 970 1030 1086 1263 Size: 185 456 239 90 60 56 177 Fragments arranged by size: 456 239 185 177 90 60 56 BspEI T'CCGG_A Cuts at: 0 1067 1212 1263 Size: 1067 145 51 Fragments arranged by size: 1067 145 51 BsrDI GCAATG_nn' Cuts at: 0 473 1263 Size: 473 790 BstAPI GCAn_nnn'nTGC Cuts at: 0 99 1159 1263 Size: 99 1060 104 Fragments arranged by size: 1060 104 99 BstYI r'GATC_y Cuts at: 0 1196 1263 Size: 1196 67 Bsu36I CC'TnA_GG Cuts at: 0 718 1263 Size: 718 545 BtgI C'CryG_G Cuts at: 0 26 528 1263 Size: 26 502 735 Fragments arranged by size: 735 502 26 BtsI GCAGTG_nn' Cuts at: 0 414 1263 Size: 414 849 ClaI AT'CG_AT Cuts at: 0 339 1263 Size: 339 924 EaeI y'GGCC_r Cuts at: 0 128 1263 Size: 128 1135 EagI C'GGCC_G Cuts at: 0 128 1263 Size: 128 1135 EarI CTCTTCn'nnn_ Cuts at: 0 761 793 1263 Size: 761 32 470 Fragments arranged by size: 761 470 32 EcoICRI GAG'CTC Cuts at: 0 183 1263 Size: 183 1080 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 808 817 847 1097 1263 Size: 306 502 9 30 250 166 Fragments arranged by size: 502 306 250 166 30 9 EcoO109I rG'GnC_Cy Cuts at: 0 49 1263 Size: 49 1214 EcoRV GAT'ATC Cuts at: 0 1122 1263 Size: 1122 141 FspI TGC'GCA Cuts at: 0 610 1263 Size: 610 653 HaeII r_GCGC'y Cuts at: 0 901 1263 Size: 901 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 901 933 936 968 989 1021 1027 1059 Size: 901 32 3 32 21 32 6 32 Cuts at: 1059 1263 Size: 204 Fragments arranged by size: 901 204 32 32 32 32 21 6 3 HincII GTy'rAC Cuts at: 0 252 1263 Size: 252 1011 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 524 1263 Size: 524 739 MslI CAynn'nnrTG Cuts at: 0 601 1263 Size: 601 662 MspA1I CmG'CkG Cuts at: 0 138 825 1263 Size: 138 687 438 Fragments arranged by size: 687 438 138 NcoI C'CATG_G Cuts at: 0 528 1263 Size: 528 735 NsiI A_TGCA'T Cuts at: 0 488 1263 Size: 488 775 NspI r_CATG'y Cuts at: 0 486 600 1263 Size: 486 114 663 Fragments arranged by size: 663 486 114 PasI CC'CwG_GG Cuts at: 0 1023 1263 Size: 1023 240 PflMI CCAn_nnn'nTGG Cuts at: 0 555 1263 Size: 555 708 PfoI T'CCnGG_A Cuts at: 0 1235 1263 Size: 1235 28 PshAI GACnn'nnGTC Cuts at: 0 445 1263 Size: 445 818 PspOMI G'GGCC_C Cuts at: 0 1026 1263 Size: 1026 237 PstI C_TGCA'G Cuts at: 0 940 1263 Size: 940 323 PvuI CG_AT'CG Cuts at: 0 438 1263 Size: 438 825 PvuII CAG'CTG Cuts at: 0 825 1263 Size: 825 438 SacI G_AGCT'C Cuts at: 0 185 1263 Size: 185 1078 SfcI C'TryA_G Cuts at: 0 840 936 1263 Size: 840 96 327 Fragments arranged by size: 840 327 96 SmaI CCC'GGG Cuts at: 0 676 1263 Size: 676 587 SmlI C'TyrA_G Cuts at: 0 655 1263 Size: 655 608 SspI AAT'ATT Cuts at: 0 782 1263 Size: 782 481 StyI C'CwwG_G Cuts at: 0 528 1263 Size: 528 735 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3 133 1263 Size: 3 130 1130 Fragments arranged by size: 1130 130 3 TatI w'GTAC_w Cuts at: 0 512 1263 Size: 512 751 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 318 350 1263 Size: 318 32 913 Fragments arranged by size: 913 318 32 XmaI C'CCGG_G Cuts at: 0 674 1263 Size: 674 589 XmnI GAAnn'nnTTC Cuts at: 0 94 118 1263 Size: 94 24 1145 Fragments arranged by size: 1145 94 24 Enzymes that do cut: AccI AcuI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BciVI BfrBI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrDI BstAPI BstYI Bsu36I BtgI BtsI ClaI EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I HincII MmeI MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI PshAI PspOMI PstI PvuI PvuII SacI SfcI SmaI SmlI SspI StyI TaqII TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BglI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI CspCI DraI DraIII DrdI EciI EcoNI EcoRI FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI ZraI