(Circular) (Six-base) MAPSORT of: pFN21AE1066.seq Check: 4032 from: 1 to: 6181 pFN21AE1066 6181 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3404 3404 Size: 6181 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 3524 1937 Size: 262 1325 4594 Fragments arranged by size: 4594 1325 262 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2439 2655 2739 4244 5628 5896 1632 Size: 807 216 84 1505 1384 268 1917 Fragments arranged by size: 1917 1505 1384 807 268 216 84 AfeI AGC'GCT Cuts at: 2129 2129 Size: 6181 AflII C'TTAA_G Cuts at: 820 1017 2258 2551 820 Size: 197 1241 293 4450 Fragments arranged by size: 4450 1241 293 197 AflIII A'CryG_T Cuts at: 1203 5080 6057 1203 Size: 3877 977 1327 Fragments arranged by size: 3877 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 2753 4910 2753 Size: 2157 4024 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6181 AlwNI CAG_nnn'CTG Cuts at: 3924 5496 3924 Size: 1572 4609 ApaI G_GGCC'C Cuts at: 2061 3186 2061 Size: 1125 5056 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 2276 2413 3002 3032 3097 3221 3490 3716 Size: 783 137 589 30 65 124 269 226 Cuts at: 3716 3868 5977 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 783 589 269 226 152 137 124 65 30 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2031 2073 3039 3506 1870 Size: 73 88 42 966 467 4545 Fragments arranged by size: 4545 966 467 88 73 42 AvrII C'CTAG_G Cuts at: 3169 3169 Size: 6181 BamHI G'GATC_C Cuts at: 3229 3511 3878 3229 Size: 282 367 5532 Fragments arranged by size: 5532 367 282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2475 3502 4957 618 Size: 325 217 627 688 1027 1455 1842 Fragments arranged by size: 1842 1455 1027 688 627 325 217 BanII G_rGCy'C Cuts at: 1391 2032 2061 2607 3186 3500 1391 Size: 641 29 546 579 314 4072 Fragments arranged by size: 4072 641 579 546 314 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2661 928 Size: 971 762 4448 Fragments arranged by size: 4448 971 762 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3423 3457 4372 4406 1903 Size: 34 1486 34 915 34 3678 Fragments arranged by size: 3678 1486 915 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4073 5283 4073 Size: 1210 4971 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4792 136 Size: 107 122 71 631 605 3120 1525 Fragments arranged by size: 3120 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6176 6176 Size: 6181 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 2061 3186 3302 4242 5398 1790 Size: 271 1125 116 940 1156 2573 Fragments arranged by size: 2573 1156 1125 940 271 116 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2010 3316 3343 Size: 416 53 83 399 24 593 1306 27 Cuts at: 3343 3912 4870 442 Size: 569 958 1753 Fragments arranged by size: 1753 1306 958 593 569 416 399 83 53 27 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2626 2626 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2680 3264 4841 1239 Size: 821 620 584 1577 2579 Fragments arranged by size: 2579 1577 821 620 584 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3227 4306 5171 5469 3227 Size: 1079 865 298 3939 Fragments arranged by size: 3939 1079 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2646 4844 882 Size: 617 1147 2198 2219 Fragments arranged by size: 2219 2198 1147 617 BsaAI yAC'GTr Cuts at: 493 1204 2116 3972 493 Size: 711 912 1856 2702 Fragments arranged by size: 2702 1856 912 711 BsaBI GATnn'nnATC Cuts at: 2973 6175 2973 Size: 3202 2979 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3386 3988 4370 Size: 53 83 186 936 476 1377 602 382 Cuts at: 4370 5005 275 Size: 635 1451 Fragments arranged by size: 1451 1377 936 635 602 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4614 5012 5286 5433 5844 4614 Size: 398 274 147 411 4951 Fragments arranged by size: 4951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 1178 Size: 30 165 30 693 30 5233 Fragments arranged by size: 5233 693 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2870 3224 3418 1186 Size: 1684 354 194 3949 Fragments arranged by size: 3949 1684 354 194 BseYI C'CCAG_C Cuts at: 2847 5384 5880 2847 Size: 2537 496 3148 Fragments arranged by size: 3148 2537 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3432 1163 Size: 573 1696 3912 Fragments arranged by size: 3912 1696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2066 2212 2218 3631 4392 5420 Size: 275 186 65 146 6 1413 761 1028 Cuts at: 5420 1540 Size: 2301 Fragments arranged by size: 2301 1413 1028 761 275 186 146 65 6 BsiHKAI G_wGCw'C Cuts at: 2470 2607 3380 4242 4327 5398 2470 Size: 137 773 862 85 1071 3253 Fragments arranged by size: 3253 1071 862 773 137 85 BsmI GAATG_Cn' Cuts at: 3064 3692 3785 3064 Size: 628 93 5460 Fragments arranged by size: 5460 628 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 2016 Size: 6181 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2061 2470 2607 3186 3302 3380 Size: 399 242 29 409 137 579 116 78 Cuts at: 3380 3500 4242 4327 5398 1391 Size: 120 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 409 399 242 137 120 116 85 78 29 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 4070 5800 4070 Size: 1730 4451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2174 3165 3404 3536 844 Size: 1330 991 239 132 3489 Fragments arranged by size: 3489 1330 991 239 132 BsrBI CCG'CTC Cuts at: 1394 1823 2068 2187 3261 4068 1394 Size: 429 245 119 1074 807 3507 Fragments arranged by size: 3507 1074 807 429 245 119 BsrDI GCAATG_nn' Cuts at: 65 1248 4676 4850 65 Size: 1183 3428 174 1396 Fragments arranged by size: 3428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2154 4825 5012 1700 Size: 110 344 2671 187 2869 Fragments arranged by size: 2869 2671 344 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6181 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 2522 3445 1184 Size: 1338 923 3920 Fragments arranged by size: 3920 1338 923 BstYI r'GATC_y Cuts at: 1972 3229 3511 3878 4263 4280 5732 5818 5830 Size: 1257 282 367 385 17 1452 86 12 Cuts at: 5830 6176 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1257 385 367 346 282 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3128 3128 Size: 6181 BtgI C'CryG_G Cuts at: 513 1065 2004 2091 3216 513 Size: 552 939 87 1125 3478 Fragments arranged by size: 3478 1125 939 552 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3294 3382 487 Size: 683 56 2068 88 3286 Fragments arranged by size: 3286 2068 683 88 56 BtsI GCAGTG_nn' Cuts at: 3051 3116 3415 3697 4491 4511 3051 Size: 65 299 282 794 20 4721 Fragments arranged by size: 4721 794 299 282 65 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3460 3485 3460 Size: 25 6156 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5188 809 Size: 507 3872 1802 Fragments arranged by size: 3872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 2209 3628 Size: 54 1425 50 275 18 233 146 1419 Cuts at: 3628 4517 6146 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1419 889 275 233 146 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2063 2209 3628 1537 Size: 275 251 146 1419 4090 Fragments arranged by size: 4090 1419 275 251 146 EarI CTCTTCn'nnn_ Cuts at: 1353 4111 1353 Size: 2758 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4768 5142 5288 387 Size: 1064 138 3179 374 146 1280 Fragments arranged by size: 3179 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2605 2605 Size: 6181 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2439 2655 2680 2739 3264 4244 Size: 393 428 379 216 25 59 525 980 Cuts at: 4244 4841 5628 5896 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 980 787 597 525 428 393 379 268 216 59 25 EcoO109I rG'GnC_Cy Cuts at: 2058 2077 2798 2876 3182 3183 2058 Size: 19 721 78 306 1 5056 Fragments arranged by size: 5056 721 306 78 19 1 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 EcoRV GAT'ATC Cuts at: 3340 3340 Size: 6181 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 2039 2131 5328 1678 Size: 361 92 3197 2531 Fragments arranged by size: 3197 2531 361 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2308 2340 2494 2526 2739 Size: 32 135 32 736 32 154 32 213 Cuts at: 2739 2771 4822 4854 4896 4928 1373 Size: 32 2051 32 42 32 2626 Fragments arranged by size: 2626 2051 736 213 154 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2947 3525 3771 669 Size: 988 281 1009 578 246 3079 Fragments arranged by size: 3079 1009 988 578 281 246 HindIII A'AGCT_T Cuts at: 748 2453 2711 3462 748 Size: 1705 258 751 3467 Fragments arranged by size: 3467 1705 751 258 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5295 5479 1305 Size: 3990 184 2007 Fragments arranged by size: 3990 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4359 Fragments arranged by size: 4359 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4139 4498 4657 518 Size: 648 2973 359 159 2042 Fragments arranged by size: 2973 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2847 2941 3597 4274 5422 5667 1606 Size: 531 710 94 656 677 1148 245 2120 Fragments arranged by size: 2120 1148 710 677 656 531 245 94 NaeI GCC'GGC Cuts at: 2156 2156 Size: 6181 NcoI C'CATG_G Cuts at: 513 1065 2004 3216 513 Size: 552 939 1212 3478 Fragments arranged by size: 3478 1212 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NgoMIV G'CCGG_C Cuts at: 2154 2154 Size: 6181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NruI TCG'CGA Cuts at: 3401 3401 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 3095 3539 5084 1132 Size: 97 1866 444 1545 2229 Fragments arranged by size: 2229 1866 1545 444 97 PasI CC'CwG_GG Cuts at: 1348 2872 1348 Size: 1524 4657 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 3355 1108 Size: 2247 3934 PfoI T'CCnGG_A Cuts at: 3232 3232 Size: 6181 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4282 5763 5795 4250 Size: 32 1481 32 4636 Fragments arranged by size: 4636 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2077 2077 Size: 6181 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PspOMI G'GGCC_C Cuts at: 2057 3182 2057 Size: 1125 5056 PspXI vC'TCGA_Gb Cuts at: 3039 3039 Size: 6181 PstI C_TGCA'G Cuts at: 830 3436 3533 830 Size: 2606 97 3478 Fragments arranged by size: 3478 2606 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2847 2941 2847 Size: 94 6087 SacI G_AGCT'C Cuts at: 2607 2607 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SexAI A'CCwGG_T Cuts at: 2079 2526 3144 2079 Size: 447 618 5116 Fragments arranged by size: 5116 618 447 SfcI C'TryA_G Cuts at: 826 1045 2842 3432 3529 4664 5345 5536 826 Size: 219 1797 590 97 1135 681 191 1471 Fragments arranged by size: 1797 1471 1135 681 590 219 191 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 2154 1700 Size: 454 5727 SmaI CCC'GGG Cuts at: 1872 2033 2075 3508 1872 Size: 161 42 1433 4545 Fragments arranged by size: 4545 1433 161 42 SmlI C'TyrA_G Cuts at: 820 1017 1943 2258 2551 3039 3206 4285 5186 Size: 197 926 315 293 488 167 1079 901 Cuts at: 5186 5448 820 Size: 262 1553 Fragments arranged by size: 1553 1079 926 901 488 315 293 262 197 167 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SrfI GCCC'GGGC Cuts at: 2033 2033 Size: 6181 SspI AAT'ATT Cuts at: 5 52 3067 4105 5 Size: 47 3015 1038 2081 Fragments arranged by size: 3015 2081 1038 47 StuI AGG'CCT Cuts at: 1517 2424 1517 Size: 907 5274 StyI C'CwwG_G Cuts at: 513 1065 2004 2436 2801 3169 3216 3620 513 Size: 552 939 432 365 368 47 404 3074 Fragments arranged by size: 3074 939 552 432 404 368 365 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2839 3395 3409 4208 4225 4378 4563 Size: 1255 1259 556 14 799 17 153 185 Cuts at: 4563 5008 325 Size: 445 1498 Fragments arranged by size: 1498 1259 1255 799 556 445 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4427 6139 96 Size: 274 80 33 51 1443 2450 1712 138 Fragments arranged by size: 2450 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6141 6173 6141 Size: 32 6149 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3223 3223 Size: 6181 XhoI C'TCGA_G Cuts at: 1943 3039 1943 Size: 1096 5085 XmaI C'CCGG_G Cuts at: 1870 2031 2073 3506 1870 Size: 161 42 1433 4545 Fragments arranged by size: 4545 1433 161 42 XmnI GAAnn'nnTTC Cuts at: 2277 2424 4310 2277 Size: 147 1886 4148 Fragments arranged by size: 4148 1886 147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NarI NsiI PacI PmlI PsrI RsrII SacII SanDI SapI SfiI SfoI SwaI