Restriction map for pFN21AE1066
 (Linear) (Six-base) MAPSORT of: KIEE1066.seq  Check: 2956  from: 1  to: 1476



KIEE1066.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1399    1476 
   Size:       1399      77
 
AccI GT'mk_AC

Cuts at:      0     194    1476 
   Size:        194    1282
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     434     650     734    1476 
   Size:        434     216      84     742

  Fragments arranged by size: 

                 742     434     216      84
 
AfeI AGC'GCT

Cuts at:      0     124    1476 
   Size:        124    1352
 
AflII C'TTAA_G

Cuts at:      0     253     546    1476 
   Size:        253     293     930

  Fragments arranged by size: 

                 930     293     253
 
AhdI GACnn_n'nnGTC

Cuts at:      0     748    1476 
   Size:        748     728
 
ApaI G_GGCC'C

Cuts at:      0      56    1181    1476 
   Size:         56    1125     295

  Fragments arranged by size: 

                1125     295      56
 
ApoI r'AATT_y

Cuts at:      0     271     408     997    1027    1092    1216    1476 
   Size:        271     137     589      30      65     124     260

  Fragments arranged by size: 

                 589     271     260     137     124      65      30
 
AvaI C'yCGr_G

Cuts at:      0      26      68    1034    1476 
   Size:         26      42     966     442

  Fragments arranged by size: 

                 966     442      42      26
 
AvrII C'CTAG_G

Cuts at:      0    1164    1476 
   Size:       1164     312
 
BamHI G'GATC_C

Cuts at:      0    1224    1476 
   Size:       1224     252
 
BanI G'GyrC_C

Cuts at:      0     470    1476 
   Size:        470    1006
 
BanII G_rGCy'C

Cuts at:      0      27      56     602    1181    1476 
   Size:         27      29     546     579     295

  Fragments arranged by size: 

                 579     546     295      29      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     656    1476 
   Size:        656     820
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1418    1452    1476 
   Size:       1418      34      24

  Fragments arranged by size: 

                1418      34      24
 
Bme1580I G_kGCm'C

Cuts at:      0      56    1181    1297    1476 
   Size:         56    1125     116     179

  Fragments arranged by size: 

                1125     179     116      56
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       5    1311    1338    1476 
   Size:          5    1306      27     138

  Fragments arranged by size: 

                1306     138      27       5
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     621    1476 
   Size:        621     855
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     675    1259    1476 
   Size:         55     620     584     217

  Fragments arranged by size: 

                 620     584     217      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1222    1476 
   Size:       1222     254
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     641    1476 
   Size:        641     835
 
BsaAI yAC'GTr

Cuts at:      0     111    1476 
   Size:        111    1365
 
BsaBI GATnn'nnATC

Cuts at:      0     968    1476 
   Size:        968     508
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1381    1476 
   Size:          4    1377      95

  Fragments arranged by size: 

                1377      95       4
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121    1476 
   Size:         91      30    1355

  Fragments arranged by size: 

                1355      91      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     865    1219    1413    1476 
   Size:        865     354     194      63

  Fragments arranged by size: 

                 865     354     194      63
 
BseYI C'CCAG_C

Cuts at:      0     842    1476 
   Size:        842     634
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1427    1476 
   Size:       1427      49
 
BsiEI CG_ry'CG

Cuts at:      0      61     207     213    1476 
   Size:         61     146       6    1263

  Fragments arranged by size: 

                1263     146      61       6
 
BsiHKAI G_wGCw'C

Cuts at:      0     465     602    1375    1476 
   Size:        465     137     773     101

  Fragments arranged by size: 

                 773     465     137     101
 
BsmI GAATG_Cn'

Cuts at:      0    1059    1476 
   Size:       1059     417
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      11    1476 
   Size:         11    1465
 
Bsp1286I G_dGCh'C

Cuts at:      0      27      56     465     602    1181    1297    1375    1476 
   Size:         27      29     409     137     579     116      78     101

  Fragments arranged by size: 

                 579     409     137     116     101      78      29      27
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     169    1160    1399    1476 
   Size:        169     991     239      77

  Fragments arranged by size: 

                 991     239     169      77
 
BsrBI CCG'CTC

Cuts at:      0      63     182    1256    1476 
   Size:         63     119    1074     220

  Fragments arranged by size: 

                1074     220     119      63
 
BsrFI r'CCGG_y

Cuts at:      0     149    1476 
   Size:        149    1327
 
BstEII G'GTnAC_C

Cuts at:      0     517    1440    1476 
   Size:        517     923      36

  Fragments arranged by size: 

                 923     517      36
 
BstYI r'GATC_y

Cuts at:      0    1224    1476 
   Size:       1224     252
 
Bsu36I CC'TnA_GG

Cuts at:      0    1123    1476 
   Size:       1123     353
 
BtgI C'CryG_G

Cuts at:      0      86    1211    1476 
   Size:         86    1125     265

  Fragments arranged by size: 

                1125     265      86
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1289    1377    1476 
   Size:       1289      88      99

  Fragments arranged by size: 

                1289      99      88
 
BtsI GCAGTG_nn'

Cuts at:      0    1046    1111    1410    1476 
   Size:       1046      65     299      66

  Fragments arranged by size: 

                1046     299      66      65
 
DraI TTT'AAA

Cuts at:      0    1455    1476 
   Size:       1455      21
 
EaeI y'GGCC_r

Cuts at:      0      58     204    1476 
   Size:         58     146    1272

  Fragments arranged by size: 

                1272     146      58
 
EagI C'GGCC_G

Cuts at:      0      58     204    1476 
   Size:         58     146    1272

  Fragments arranged by size: 

                1272     146      58
 
EcoICRI GAG'CTC

Cuts at:      0     600    1476 
   Size:        600     876
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     434     650     675     734    1259    1476 
   Size:         55     379     216      25      59     525     217

  Fragments arranged by size: 

                 525     379     217     216      59      55      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0      53      72     793     871    1177    1178    1476 
   Size:         53      19     721      78     306       1     298

  Fragments arranged by size: 

                 721     306     298      78      53      19       1
 
EcoRV GAT'ATC

Cuts at:      0    1335    1476 
   Size:       1335     141
 
HaeII r_GCGC'y

Cuts at:      0      34     126    1476 
   Size:         34      92    1350

  Fragments arranged by size: 

                1350      92      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     303     335     489     521     734     766    1476 
   Size:        303      32     154      32     213      32     710

  Fragments arranged by size: 

                 710     303     213     154      32      32      32
 
HincII GTy'rAC

Cuts at:      0     942    1476 
   Size:        942     534
 
HindIII A'AGCT_T

Cuts at:      0     448     706    1457    1476 
   Size:        448     258     751      19

  Fragments arranged by size: 

                 751     448     258      19
 
MspA1I CmG'CkG

Cuts at:      0     132     842     936    1476 
   Size:        132     710      94     540

  Fragments arranged by size: 

                 710     540     132      94
 
NaeI GCC'GGC

Cuts at:      0     151    1476 
   Size:        151    1325
 
NcoI C'CATG_G

Cuts at:      0    1211    1476 
   Size:       1211     265
 
NgoMIV G'CCGG_C

Cuts at:      0     149    1476 
   Size:        149    1327
 
NruI TCG'CGA

Cuts at:      0    1396    1476 
   Size:       1396      80
 
NspI r_CATG'y

Cuts at:      0    1090    1476 
   Size:       1090     386
 
PasI CC'CwG_GG

Cuts at:      0     867    1476 
   Size:        867     609
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1350    1476 
   Size:       1350     126
 
PfoI T'CCnGG_A

Cuts at:      0    1227    1476 
   Size:       1227     249
 
PpuMI rG'GwC_Cy

Cuts at:      0      72    1476 
   Size:         72    1404
 
PspOMI G'GGCC_C

Cuts at:      0      52    1177    1476 
   Size:         52    1125     299

  Fragments arranged by size: 

                1125     299      52
 
PspXI vC'TCGA_Gb

Cuts at:      0    1034    1476 
   Size:       1034     442
 
PstI C_TGCA'G

Cuts at:      0    1431    1476 
   Size:       1431      45
 
PvuII CAG'CTG

Cuts at:      0     842     936    1476 
   Size:        842      94     540

  Fragments arranged by size: 

                 842     540      94
 
SacI G_AGCT'C

Cuts at:      0     602    1476 
   Size:        602     874
 
SexAI A'CCwGG_T

Cuts at:      0      74     521    1139    1476 
   Size:         74     447     618     337

  Fragments arranged by size: 

                 618     447     337      74
 
SfcI C'TryA_G

Cuts at:      0     837    1427    1476 
   Size:        837     590      49

  Fragments arranged by size: 

                 837     590      49
 
SgrAI Cr'CCGG_yG

Cuts at:      0     149    1476 
   Size:        149    1327
 
SmaI CCC'GGG

Cuts at:      0      28      70    1476 
   Size:         28      42    1406

  Fragments arranged by size: 

                1406      42      28
 
SmlI C'TyrA_G

Cuts at:      0     253     546    1034    1201    1476 
   Size:        253     293     488     167     275

  Fragments arranged by size: 

                 488     293     275     253     167
 
SrfI GCCC'GGGC

Cuts at:      0      28    1476 
   Size:         28    1448
 
SspI AAT'ATT

Cuts at:      0    1062    1476 
   Size:       1062     414
 
StuI AGG'CCT

Cuts at:      0     419    1476 
   Size:        419    1057
 
StyI C'CwwG_G

Cuts at:      0     431     796    1164    1211    1476 
   Size:        431     365     368      47     265

  Fragments arranged by size: 

                 431     368     365     265      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     834    1390    1404    1476 
   Size:        834     556      14      72

  Fragments arranged by size: 

                 834     556      72      14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1218    1476 
   Size:       1218     258
 
XhoI C'TCGA_G

Cuts at:      0    1034    1476 
   Size:       1034     442
 
XmaI C'CCGG_G

Cuts at:      0      26      68    1476 
   Size:         26      42    1408

  Fragments arranged by size: 

                1408      42      26
 
XmnI GAAnn'nnTTC

Cuts at:      0     272     419    1476 
   Size:        272     147    1057

  Fragments arranged by size: 

                1057     272     147
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII     AhdI     ApaI     ApoI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI   BstEII    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     EaeI     EagI  EcoICRI  Eco57MI EcoO109I
    EcoRV    HaeII    Hin4I   HincII  HindIII   MspA1I     NaeI     NcoI
   NgoMIV     NruI     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI
    PspXI     PstI    PvuII     SacI    SexAI     SfcI    SgrAI     SmaI
     SmlI     SrfI     SspI     StuI     StyI    TaqII     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI   AflIII     AgeI     AleI     AlfI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI     BaeI     BbeI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
   Bpu10I    BsaWI    BsiWI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I     ClaI    CspCI   DraIII
     DrdI     EarI     EciI    EcoNI    EcoRI     FalI     FseI     FspI
    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NarI     NdeI     NheI     NotI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SnaBI     SpeI     SphI     SwaI     TatI     TstI  Tth111I
     XbaI     ZraI
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