(Circular) (Six-base) MAPSORT of: pFN21AE0378.seq Check: 2433 from: 1 to: 6448 pFN21AE0378 6448 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4258 278 Size: 53 83 186 936 2722 2468 Fragments arranged by size: 2722 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 3791 1937 Size: 1188 666 4594 Fragments arranged by size: 4594 1188 666 Acc65I G'GTAC_C Cuts at: 3769 3769 Size: 6448 AclI AA'CG_TT Cuts at: 1563 3196 4575 4948 1563 Size: 1633 1379 373 3063 Fragments arranged by size: 3063 1633 1379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2511 4511 5895 6163 1632 Size: 879 2000 1384 268 1917 Fragments arranged by size: 2000 1917 1384 879 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6251 AflIII A'CryG_T Cuts at: 1203 3140 3609 5347 6324 1203 Size: 1937 469 1738 977 1327 Fragments arranged by size: 1937 1738 1327 977 469 AgeI A'CCGG_T Cuts at: 5279 5279 Size: 6448 AhdI GACnn_n'nnGTC Cuts at: 5177 5177 Size: 6448 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6448 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2187 2190 1184 Size: 1003 3 5442 Fragments arranged by size: 5442 1003 3 AlwNI CAG_nnn'CTG Cuts at: 3341 4191 5763 3341 Size: 850 1572 4026 Fragments arranged by size: 4026 1572 850 ApaLI G'TGCA_C Cuts at: 4505 5661 4505 Size: 1156 5292 ApoI r'AATT_y Cuts at: 1493 2021 2102 2257 2342 2352 2732 3259 3566 Size: 528 81 155 85 10 380 527 307 Cuts at: 3566 3757 3983 4135 6244 1493 Size: 191 226 152 2109 1697 Fragments arranged by size: 2109 1697 528 527 380 307 226 191 155 152 85 81 10 AseI AT'TA_AT Cuts at: 160 1032 5002 160 Size: 872 3970 1606 Fragments arranged by size: 3970 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 AvaI C'yCGr_G Cuts at: 1870 1943 3773 1870 Size: 73 1830 4545 Fragments arranged by size: 4545 1830 73 BamHI G'GATC_C Cuts at: 3778 4145 3778 Size: 367 6081 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3439 3769 5224 618 Size: 325 217 627 1652 330 1455 1842 Fragments arranged by size: 1842 1652 1455 627 330 325 217 BanII G_rGCy'C Cuts at: 1391 3767 1391 Size: 2376 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5477 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4639 4673 1903 Size: 34 2702 34 3678 Fragments arranged by size: 3678 2702 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4340 5550 4340 Size: 1210 5238 BclI T'GATC_A Cuts at: 1548 2191 3598 1548 Size: 643 1407 4398 Fragments arranged by size: 4398 1407 643 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5059 136 Size: 107 122 71 631 605 3387 1525 Fragments arranged by size: 3387 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2932 6443 2932 Size: 3511 2937 BlpI GC'TnA_GC Cuts at: 3865 3865 Size: 6448 Bme1580I G_kGCm'C Cuts at: 1790 4509 5665 1790 Size: 2719 1156 2573 Fragments arranged by size: 2719 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6448 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 3516 4179 Size: 416 53 83 399 24 1973 126 663 Cuts at: 4179 5137 442 Size: 958 1753 Fragments arranged by size: 1973 1753 958 663 416 399 126 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6448 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5108 1239 Size: 3869 2579 Bpu10I CC'TnA_GC Cuts at: 2328 2328 Size: 6448 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3701 4573 5438 5736 3701 Size: 872 865 298 4413 Fragments arranged by size: 4413 872 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5111 882 Size: 617 3612 2219 Fragments arranged by size: 3612 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4239 493 Size: 711 3035 2702 Fragments arranged by size: 3035 2702 711 BsaBI GATnn'nnATC Cuts at: 6442 6442 Size: 6448 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4255 4637 5272 275 Size: 53 83 186 936 2722 382 635 1451 Fragments arranged by size: 2722 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2625 4881 5279 5553 5700 6111 2625 Size: 2256 398 274 147 411 2962 Fragments arranged by size: 2962 2256 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6223 Fragments arranged by size: 6223 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 1186 Size: 1294 5154 BseYI C'CCAG_C Cuts at: 2862 5651 6147 2862 Size: 2789 496 3163 Fragments arranged by size: 3163 2789 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2979 1163 Size: 573 1243 4632 Fragments arranged by size: 4632 1243 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3898 4659 5687 1540 Size: 275 186 1897 761 1028 2301 Fragments arranged by size: 2301 1897 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4509 4594 5665 4509 Size: 85 1071 5292 Fragments arranged by size: 5292 1071 85 BsmI GAATG_Cn' Cuts at: 2918 3959 4052 2918 Size: 1041 93 5314 Fragments arranged by size: 5314 1041 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3767 4509 4594 5665 1391 Size: 399 1977 742 85 1071 2174 Fragments arranged by size: 2174 1977 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2625 6111 2625 Size: 3486 2962 BspHI T'CATG_A Cuts at: 4337 6067 4337 Size: 1730 4718 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3803 844 Size: 2959 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4335 1394 Size: 429 2512 3507 Fragments arranged by size: 3507 2512 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2670 3512 4943 5117 65 Size: 1183 1422 842 1431 174 1396 Fragments arranged by size: 1431 1422 1396 1183 842 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5092 5279 1700 Size: 110 3282 187 2869 Fragments arranged by size: 3282 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6448 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6448 BssSI C'ACGA_G Cuts at: 4508 5520 4508 Size: 1012 5436 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6448 BstBI TT'CG_AA Cuts at: 5252 5252 Size: 6448 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6448 BstYI r'GATC_y Cuts at: 1972 2932 3337 3778 4145 4530 4547 5999 6085 Size: 960 405 441 367 385 17 1452 86 Cuts at: 6085 6097 6443 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 960 441 405 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5896 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5709 Fragments arranged by size: 5709 683 56 BtsI GCAGTG_nn' Cuts at: 2202 3964 4758 4778 2202 Size: 1762 794 20 3872 Fragments arranged by size: 3872 1762 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6448 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6413 DraI TTT'AAA Cuts at: 2421 2635 2688 3752 2421 Size: 214 53 1064 5117 Fragments arranged by size: 5117 1064 214 53 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5455 809 Size: 507 4139 1802 Fragments arranged by size: 4139 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3895 4784 6413 Size: 54 1425 50 275 18 2065 889 1629 Cuts at: 6413 8 Size: 43 Fragments arranged by size: 2065 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3895 1537 Size: 275 2083 4090 Fragments arranged by size: 4090 2083 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2466 4378 1353 Size: 1113 1912 3423 Fragments arranged by size: 3423 1912 1113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5035 5409 5555 387 Size: 1064 138 3446 374 146 1280 Fragments arranged by size: 3446 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2511 4511 5108 5895 6163 1239 Size: 393 879 2000 597 787 268 1524 Fragments arranged by size: 2000 1524 879 787 597 393 268 EcoRI G'AATT_C Cuts at: 2257 2342 3757 4135 2257 Size: 85 1415 378 4570 Fragments arranged by size: 4570 1415 378 85 FspI TGC'GCA Cuts at: 4954 4954 Size: 6448 HaeII r_GCGC'y Cuts at: 1678 5595 1678 Size: 3917 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2659 2691 5089 5121 5163 Size: 32 135 32 1087 32 2398 32 42 Cuts at: 5163 5195 1373 Size: 32 2626 Fragments arranged by size: 2626 2398 1087 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3792 4038 669 Size: 988 281 1854 246 3079 Fragments arranged by size: 3079 1854 988 281 246 HindIII A'AGCT_T Cuts at: 748 3223 748 Size: 2475 3973 HpaI GTT'AAC Cuts at: 4038 4038 Size: 6448 KpnI G_GTAC'C Cuts at: 3773 3773 Size: 6448 MfeI C'AATT_G Cuts at: 4047 4047 Size: 6448 MluI A'CGCG_T Cuts at: 6324 6324 Size: 6448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2702 5562 5746 1305 Size: 1397 2860 184 2007 Fragments arranged by size: 2860 2007 1397 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4626 Fragments arranged by size: 4626 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4406 4765 4924 518 Size: 648 3240 359 159 2042 Fragments arranged by size: 3240 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3864 4541 5689 5934 1606 Size: 2258 677 1148 245 2120 Fragments arranged by size: 2258 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5896 NdeI CA'TA_TG Cuts at: 387 3478 387 Size: 3091 3357 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6448 NotI GC'GGCC_GC Cuts at: 3895 3895 Size: 6448 NspI r_CATG'y Cuts at: 1132 1229 2051 2085 2336 2663 3144 3806 5351 Size: 97 822 34 251 327 481 662 1545 Cuts at: 5351 1132 Size: 2229 Fragments arranged by size: 2229 1545 822 662 481 327 251 97 34 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6448 PciI A'CATG_T Cuts at: 3140 5347 3140 Size: 2207 4241 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6448 PmeI GTTT'AAAC Cuts at: 3752 3752 Size: 6448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4517 4549 6030 6062 4517 Size: 32 1481 32 4903 Fragments arranged by size: 4903 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6448 PsiI TTA'TAA Cuts at: 4018 4018 Size: 6448 PstI C_TGCA'G Cuts at: 830 2179 3800 830 Size: 1349 1621 3478 Fragments arranged by size: 3478 1621 1349 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6448 SalI G'TCGA_C Cuts at: 1936 3790 1936 Size: 1854 4594 SbfI CC_TGCA'GG Cuts at: 3800 3800 Size: 6448 ScaI AGT'ACT Cuts at: 4696 6408 4696 Size: 1712 4736 SfcI C'TryA_G Cuts at: 826 1045 2175 3126 3796 4931 5612 5803 826 Size: 219 1130 951 670 1135 681 191 1471 Fragments arranged by size: 1471 1135 1130 951 681 670 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6448 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6448 SmaI CCC'GGG Cuts at: 1872 3775 1872 Size: 1903 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3680 4552 5453 5715 820 Size: 197 926 1737 872 901 262 1553 Fragments arranged by size: 1737 1553 926 901 872 262 197 SnaBI TAC'GTA Cuts at: 493 4239 493 Size: 3746 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6448 SphI G_CATG'C Cuts at: 1132 2051 3806 1132 Size: 919 1755 3774 Fragments arranged by size: 3774 1755 919 SspI AAT'ATT Cuts at: 5 52 4372 5 Size: 47 4320 2081 Fragments arranged by size: 4320 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6448 StyI C'CwwG_G Cuts at: 513 1065 3887 513 Size: 552 2822 3074 Fragments arranged by size: 3074 2822 552 SwaI ATTT'AAAT Cuts at: 2688 2688 Size: 6448 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4475 4492 4645 4830 5275 325 Size: 1255 2895 17 153 185 445 1498 Fragments arranged by size: 2895 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4694 6406 96 Size: 274 80 33 51 1443 2717 1712 138 Fragments arranged by size: 2717 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6408 6440 6408 Size: 32 6416 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6448 XbaI T'CTAG_A Cuts at: 3784 3784 Size: 6448 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6448 XmaI C'CCGG_G Cuts at: 1870 3773 1870 Size: 1903 4545 XmnI GAAnn'nnTTC Cuts at: 2934 3012 4577 2934 Size: 78 1565 4805 Fragments arranged by size: 4805 1565 78 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4256 276 Size: 53 83 186 936 2722 2468 Fragments arranged by size: 2722 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI