Restriction map for
pFN21AB6814
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
Fnu4HI FseI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI KspI MaeII
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TaqI TseI Tth111I
TthHB8I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AlwI 126 GGATC GGATC(4/5)
AspHI 492 GAGCTC G[AT]GC[AT]^C
BanII 492 GAGCTC G[GA]GC[CT]^C
BbsI 222 GAAGAC GAAGAC(2/6)
BglII 278 AGATCT A^GATCT
BmyI 492 GAGCTC G[AGT]GC[ACT]^C
BpuAI 222 GAAGAC GAAGAC(2/6)
BsaBI 139 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 492 GAGCTC G[AT]GC[AT]^C
BsiYI 112 CCAGGATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 112 CCAGGATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 492 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 517 TGTACA T^GTACA
BsrGI 517 TGTACA T^GTACA
BstF5I 195 CATCC GGATG(2/0)
BstYI 278 AGATCT [GA]^GATC[CT]
DdeI 282 CTCAG C^T[ACGT]AG
FokI 195 CATCC GGATG(9/13)
HinfI 357 GATTC G^A[ACGT]TC
HphI 432 TCACC GGTGA(8/7)
MamI 139 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 278 AGATCT [GA]^GATC[CT]
MwoI 418 GCTGTTCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 454 CATATG CA^TATG
PflMI 112 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 492 GAGCTC GAGCT^C
SfcI 524 CTATAG C^T[GA][CT]AG
SmlI 331 CTTGAG C^T[CT][GA]AG
SspBI 517 TGTACA T^GTACA
TfiI 357 GATTC G^A[AT]TC
TspRI 218 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 112 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 147 TCTAGA T^CTAGA
XhoII 278 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 320 AAATTC [GA]^AATT[CT]
505 AAATTT
ApoI 320 AAATTC [GA]^AATT[CT]
505 AAATTT
BsmAI 446 GAGAC GTCTC(1/5)
459 GAGAC
BspHI 404 TCATGA T^CATGA
533 TCATGA
BsrI 55 ACTGG ACTGG(1/-1)
481 CCAGT
BssKI 25 CCTGG ^CC[ACGT]GG
112 CCAGG
BstNI 25 CCTGG CC^[AT]GG
112 CCAGG
BstXI 38 CCAAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
346 CCAAGTGGCTGG
DpnII 127 GATC ^GATC
279 GATC
EarI 443 GAAGAG CTCTTC(1/4)
487 GAAGAG
EcoRII 25 CCTGG ^CC[AT]GG
112 CCAGG
Ksp632I 443 GAAGAG CTCTTC(1/4)
487 GAAGAG
MboI 127 GATC ^GATC
279 GATC
MnlI 22 CCTC CCTC(7/6)
181 CCTC
MseI 370 TTAA T^TAA
467 TTAA
MslI 433 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
450 CACCCATATG
MvaI 25 CCTGG CC^[AT]GG
112 CCAGG
NdeII 127 GATC ^GATC
279 GATC
NlaIV 172 GGTTCC GG[ACGT]^[ACGT]CC
342 GGAACC
RcaI 404 TCATGA T^CATGA
533 TCATGA
Sau3AI 127 GATC ^GATC
279 GATC
ScrFI 25 CCTGG CC^[ACGT]GG
112 CCAGG
Tru9I 370 TTAA T^TAA
467 TTAA
Tsp45I 78 GTCAC ^GT[GC]AC
93 GTCAC
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AfaI AluI BfaI MaeI MaeIII
MboII NlaIII RsaI Tsp509I