Restriction map for
pFN21AB7717
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MroI MseI NarI NcoI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AhdI 718 GACCGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 525 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 718 GACCGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 699 GTGCTC G[AT]GC[AT]^C
AvaI 452 CCCGGG C^[CT]CG[GA]G
BalI 253 TGGCCA TGG^CCA
BanI 1142 GGTGCC G^G[CT][GA]CC
BanII 678 GAGCCC G[GA]GC[CT]^C
BbvCI 645 CCTCAGC CCTCAGC(-5/-2)
BcgI 48 CGAAGACGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 271 CTAG C^TAG
BglII 225 AGATCT A^GATCT
BseRI 241 GAGGAG GAGGAG(10/8)
BsgI 690 GTGCAG GTGCAG(16/14)
BsiHKAI 699 GTGCTC G[AT]GC[AT]^C
BsmBI 977 CGTCTC CGTCTC(1/5)
BsoBI 452 CCCGGG C^[CT]CG[GA]G
BsrBI 1056 CCGCTC CCGCTC(-3/-3)
BsrFI 1145 GCCGGC [GA]^CCGG[CT]
BstBI 46 TTCGAA TT^CGAA
Cfr10I 1145 GCCGGC [GA]^CCGG[CT]
DraIII 1197 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 253 TGGCCA [CT]^GGCC[GA]
Eam1105I 718 GACCGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 111 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 342 CCTTGG C^C[AT][AT]GG
MaeI 271 CTAG C^TAG
MaeII 53 ACGT A^CGT
MfeI 193 CAATTG C^AATTG
MluNI 253 TGGCCA TGG^CCA
MscI 253 TGGCCA TGG^CCA
MspA1I 1138 CAGCGG C[AC]G^C[GT]G
MunI 193 CAATTG C^AATTG
NaeI 1145 GCCGGC GCC^GGC
NdeI 1103 CATATG CA^TATG
NgoMIV 1145 GCCGGC G^CCGGC
NspV 46 TTCGAA TT^CGAA
SfuI 46 TTCGAA TT^CGAA
SmaI 452 CCCGGG CCC^GGG
StyI 342 CCTTGG C^C[AT][AT]GG
XbaI 270 TCTAGA T^CTAGA
XcmI 389 CCAATGGCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 452 CCCGGG C^CCGGG
XmaI 452 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 610 CGCG CG^CG
1154 CGCG
AlwI 1083 GATCC GGATC(4/5)
325 GGATC
BbsI 49 GAAGAC GAAGAC(2/6)
537 GAAGAC
BcnI 452 CCCGG CC^[GC]GG
453 CCGGG
BmyI 678 GAGCCC G[AGT]GC[ACT]^C
699 GTGCTC
BpuAI 49 GAAGAC GAAGAC(2/6)
537 GAAGAC
Bsp1286I 678 GAGCCC G[AGT]GC[ACT]^C
699 GTGCTC
BstAPI 692 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1112 GCAGCGAATGC
BstUI 610 CGCG CG^CG
1154 CGCG
BstYI 225 AGATCT [GA]^GATC[CT]
1082 AGATCC
DraII 107 GGGACCT [GA]G^G[ACGT]CC[CT]
1010 GGGACCC
DsaI 59 CCGTGG C^C[GA][CT]GG
214 CCGTGG
EarI 76 GAAGAG CTCTTC(1/4)
198 GAAGAG
EcoO109I 107 GGGACCT [GA]G^G[ACGT]CC[CT]
1010 GGGACCC
EcoRI 746 GAATTC G^AATTC
830 GAATTC
HaeIII 160 GGCC GG^CC
254 GGCC
HapII 453 CCGG C^CGG
1146 CCGG
HindIII 959 AAGCTT A^AGCTT
1043 AAGCTT
HpaII 453 CCGG C^CGG
1146 CCGG
HphI 123 GGTGA GGTGA(8/7)
563 TCACC
Ksp632I 76 GAAGAG CTCTTC(1/4)
198 GAAGAG
MflI 225 AGATCT [GA]^GATC[CT]
1082 AGATCC
MspI 453 CCGG C^CGG
1146 CCGG
MvnI 610 CGCG CG^CG
1154 CGCG
NciI 452 CCCGG CC^[GC]GG
453 CCGGG
NlaIII 232 CATG CATG^
1003 CATG
PpuMI 107 GGGACCT [GA]G^G[AT]CC[CT]
1010 GGGACCC
TaqI 47 TCGA T^CGA
819 TCGA
TfiI 34 GAATC G^A[AT]TC
294 GATTC
TthHB8I 47 TCGA T^CGA
819 TCGA
XhoII 225 AGATCT [GA]^GATC[CT]
1082 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
AvaII BbvI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsmI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HinfI ItaI MaeIII MboI
MboII MnlI MslI MvaI MwoI
NdeII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I Tsp509I
TspRI XmnI