(Circular) (Six-base) MAPSORT of: pFN21AA1594.seq Check: 6545 from: 1 to: 7642 pFN21AA1594 7642 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5452 278 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4985 1937 Size: 3048 4594 Acc65I G'GTAC_C Cuts at: 4963 4963 Size: 7642 AclI AA'CG_TT Cuts at: 1563 3976 5769 6142 1563 Size: 2413 1793 373 3063 Fragments arranged by size: 3063 2413 1793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 4421 4638 4698 5705 7089 7357 1632 Size: 404 2385 217 60 1007 1384 268 1917 Fragments arranged by size: 2385 1917 1384 1007 404 268 217 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7445 AflIII A'CryG_T Cuts at: 1203 6541 7518 1203 Size: 5338 977 1327 Fragments arranged by size: 5338 1327 977 AgeI A'CCGG_T Cuts at: 6473 6473 Size: 7642 AhdI GACnn_n'nnGTC Cuts at: 4929 6371 4929 Size: 1442 6200 AleI CACnn'nnGTG Cuts at: 1166 4913 1166 Size: 3747 3895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3303 3436 1184 Size: 2119 133 5390 Fragments arranged by size: 5390 2119 133 AlwNI CAG_nnn'CTG Cuts at: 3022 4447 4616 5385 6957 3022 Size: 1425 169 769 1572 3707 Fragments arranged by size: 3707 1572 1425 769 169 ApaLI G'TGCA_C Cuts at: 5699 6855 5699 Size: 1156 6486 ApoI r'AATT_y Cuts at: 1493 2037 2142 3138 3296 3385 3656 3871 3908 Size: 544 105 996 158 89 271 215 37 Cuts at: 3908 4951 5177 5329 7438 1493 Size: 1043 226 152 2109 1697 Fragments arranged by size: 2109 1697 1043 996 544 271 226 215 158 152 105 89 37 AseI AT'TA_AT Cuts at: 160 1032 6196 160 Size: 872 5164 1606 Fragments arranged by size: 5164 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 AvaI C'yCGr_G Cuts at: 1870 1943 4967 1870 Size: 73 3024 4545 Fragments arranged by size: 4545 3024 73 AvrII C'CTAG_G Cuts at: 3671 3671 Size: 7642 BamHI G'GATC_C Cuts at: 2078 2828 4303 4972 5339 2078 Size: 750 1475 669 367 4381 Fragments arranged by size: 4381 1475 750 669 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4963 6418 618 Size: 325 217 627 3176 1455 1842 Fragments arranged by size: 3176 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3932 4961 1391 Size: 2541 1029 4072 Fragments arranged by size: 4072 2541 1029 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3365 4041 4517 4928 928 Size: 971 1466 676 476 411 3642 Fragments arranged by size: 3642 1466 971 676 476 411 BbvCI CC'TCA_GC Cuts at: 2922 3989 4623 2922 Size: 1067 634 5941 Fragments arranged by size: 5941 1067 634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4799 4833 5833 5867 1903 Size: 34 2862 34 1000 34 3678 Fragments arranged by size: 3678 2862 1000 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3134 5534 6744 3134 Size: 2400 1210 4032 Fragments arranged by size: 4032 2400 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7642 BfrBI ATG'CAT Cuts at: 4556 4556 Size: 7642 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6253 136 Size: 107 122 71 631 605 4581 1525 Fragments arranged by size: 4581 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4860 7637 4860 Size: 2777 4865 BlpI GC'TnA_GC Cuts at: 5059 5059 Size: 7642 Bme1580I G_kGCm'C Cuts at: 1790 2997 5703 6859 1790 Size: 1207 2706 1156 2573 Fragments arranged by size: 2706 2573 1207 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7642 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2064 2190 5373 Size: 416 53 83 399 24 647 126 3183 Cuts at: 5373 6331 442 Size: 958 1753 Fragments arranged by size: 3183 1753 958 647 416 399 126 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7642 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3283 3283 Size: 7642 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 2695 3074 3396 3626 4347 4379 4799 Size: 918 538 379 322 230 721 32 420 Cuts at: 4799 6302 1239 Size: 1503 2579 Fragments arranged by size: 2579 1503 918 721 538 420 379 322 230 32 Bpu10I CC'TnA_GC Cuts at: 2922 3989 4623 2922 Size: 1067 634 5941 Fragments arranged by size: 5941 1067 634 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3209 5767 6632 6930 3209 Size: 2558 865 298 3921 Fragments arranged by size: 3921 2558 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3200 6305 882 Size: 617 1701 3105 2219 Fragments arranged by size: 3105 2219 1701 617 BsaAI yAC'GTr Cuts at: 493 1204 5433 493 Size: 711 4229 2702 Fragments arranged by size: 4229 2702 711 BsaBI GATnn'nnATC Cuts at: 4821 7636 4821 Size: 2815 4827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5449 5831 6466 275 Size: 53 83 186 936 3916 382 635 1451 Fragments arranged by size: 3916 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4217 6075 6473 6747 6894 7305 4217 Size: 1858 398 274 147 411 4554 Fragments arranged by size: 4554 1858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7417 Fragments arranged by size: 7417 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2001 3546 1186 Size: 815 1545 5282 Fragments arranged by size: 5282 1545 815 BseYI C'CCAG_C Cuts at: 6845 7341 6845 Size: 496 7146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4829 1163 Size: 573 3093 3976 Fragments arranged by size: 3976 3093 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5092 5853 6881 1540 Size: 275 186 3091 761 1028 2301 Fragments arranged by size: 3091 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3643 3932 5703 5788 6859 3643 Size: 289 1771 85 1071 4426 Fragments arranged by size: 4426 1771 1071 289 85 BsmI GAATG_Cn' Cuts at: 3067 5153 5246 3067 Size: 2086 93 5463 Fragments arranged by size: 5463 2086 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2349 2349 Size: 7642 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2997 3643 3932 4961 5703 5788 6859 Size: 399 1207 646 289 1029 742 85 1071 Cuts at: 6859 1391 Size: 2174 Fragments arranged by size: 2174 1207 1071 1029 742 646 399 289 85 BspEI T'CCGG_A Cuts at: 7305 7305 Size: 7642 BspHI T'CATG_A Cuts at: 3292 4180 5531 7261 3292 Size: 888 1351 1730 3673 Fragments arranged by size: 3673 1730 1351 888 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3059 4997 844 Size: 2215 1938 3489 Fragments arranged by size: 3489 2215 1938 BsrBI CCG'CTC Cuts at: 1394 1823 5529 1394 Size: 429 3706 3507 Fragments arranged by size: 3706 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3781 6137 6311 65 Size: 1183 2533 2356 174 1396 Fragments arranged by size: 2533 2356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4671 6286 6473 1700 Size: 110 2861 1615 187 2869 Fragments arranged by size: 2869 2861 1615 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7642 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7642 BssSI C'ACGA_G Cuts at: 5702 6714 5702 Size: 1012 6630 BstAPI GCAn_nnn'nTGC Cuts at: 794 3022 794 Size: 2228 5414 BstBI TT'CG_AA Cuts at: 2041 2482 6446 2041 Size: 441 3964 3237 Fragments arranged by size: 3964 3237 441 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7642 BstYI r'GATC_y Cuts at: 1972 2078 2704 2828 4303 4370 4860 4972 5339 Size: 106 626 124 1475 67 490 112 367 Cuts at: 5339 5724 5741 7193 7279 7291 7637 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1475 1452 626 490 385 367 346 124 112 106 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2664 2664 Size: 7642 BtgI C'CryG_G Cuts at: 513 1065 3128 513 Size: 552 2063 5027 Fragments arranged by size: 5027 2063 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6903 Fragments arranged by size: 6903 683 56 BtsI GCAGTG_nn' Cuts at: 5158 5952 5972 5158 Size: 794 20 6828 Fragments arranged by size: 6828 794 20 ClaI AT'CG_AT Cuts at: 1554 2513 1554 Size: 959 6683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7607 DraI TTT'AAA Cuts at: 4946 4946 Size: 7642 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6649 809 Size: 507 5333 1802 Fragments arranged by size: 5333 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5089 5978 7607 Size: 54 1425 50 275 18 3259 889 1629 Cuts at: 7607 8 Size: 43 Fragments arranged by size: 3259 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5089 1537 Size: 275 3277 4090 Fragments arranged by size: 4090 3277 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2760 2848 4116 4419 5572 1353 Size: 1407 88 1268 303 1153 3423 Fragments arranged by size: 3423 1407 1268 1153 303 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6229 6603 6749 387 Size: 1064 138 4640 374 146 1280 Fragments arranged by size: 4640 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3930 3930 Size: 7642 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2157 2695 3074 3396 3626 4347 Size: 393 404 121 538 379 322 230 721 Cuts at: 4347 4379 4421 4638 4698 4799 5705 6302 7089 Size: 32 42 217 60 101 906 597 787 Cuts at: 7089 7357 1239 Size: 268 1524 Fragments arranged by size: 1524 906 787 721 597 538 404 393 379 322 268 230 217 121 101 60 42 32 EcoO109I rG'GnC_Cy Cuts at: 2385 3676 2385 Size: 1291 6351 EcoRI G'AATT_C Cuts at: 2037 2142 3871 4951 5329 2037 Size: 105 1729 1080 378 4350 Fragments arranged by size: 4350 1729 1080 378 105 EcoRV GAT'ATC Cuts at: 2631 3193 2631 Size: 562 7080 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3593 3949 4142 4879 3593 Size: 356 193 737 6356 Fragments arranged by size: 6356 737 356 193 FspI TGC'GCA Cuts at: 3828 6148 3828 Size: 2320 5322 FspAI rTGC'GCAy Cuts at: 3828 3828 Size: 7642 HaeII r_GCGC'y Cuts at: 1678 6789 1678 Size: 5111 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2011 2043 2666 2698 3474 Size: 32 135 32 439 32 623 32 776 Cuts at: 3474 3506 4559 4591 4639 4671 4915 4947 6283 Size: 32 1053 32 48 32 244 32 1336 Cuts at: 6283 6315 6357 6389 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1336 1053 776 623 439 244 135 48 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2226 4986 5232 669 Size: 988 281 288 2760 246 3079 Fragments arranged by size: 3079 2760 988 288 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7642 HpaI GTT'AAC Cuts at: 2226 5232 2226 Size: 3006 4636 KpnI G_GTAC'C Cuts at: 4967 4967 Size: 7642 MfeI C'AATT_G Cuts at: 5241 5241 Size: 7642 MluI A'CGCG_T Cuts at: 7518 7518 Size: 7642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3800 6756 6940 1305 Size: 2495 2956 184 2007 Fragments arranged by size: 2956 2495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5820 Fragments arranged by size: 5820 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4185 4913 5600 5959 6118 518 Size: 648 3019 728 687 359 159 2042 Fragments arranged by size: 3019 2042 728 687 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2746 3322 4136 5058 5735 6883 7128 1606 Size: 1140 576 814 922 677 1148 245 2120 Fragments arranged by size: 2120 1148 1140 922 814 677 576 245 NcoI C'CATG_G Cuts at: 513 1065 3128 513 Size: 552 2063 5027 Fragments arranged by size: 5027 2063 552 NdeI CA'TA_TG Cuts at: 387 3831 387 Size: 3444 4198 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7642 NotI GC'GGCC_GC Cuts at: 5089 5089 Size: 7642 NsiI A_TGCA'T Cuts at: 4558 4558 Size: 7642 NspI r_CATG'y Cuts at: 1132 1229 3480 5000 6545 1132 Size: 97 2251 1520 1545 2229 Fragments arranged by size: 2251 2229 1545 1520 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7642 PciI A'CATG_T Cuts at: 6541 6541 Size: 7642 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7642 PmeI GTTT'AAAC Cuts at: 4946 4946 Size: 7642 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5711 5743 7224 7256 5711 Size: 32 1481 32 6097 Fragments arranged by size: 6097 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2385 3676 2385 Size: 1291 6351 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7642 PsiI TTA'TAA Cuts at: 5212 5212 Size: 7642 PstI C_TGCA'G Cuts at: 830 2332 3027 4994 830 Size: 1502 695 1967 3478 Fragments arranged by size: 3478 1967 1502 695 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7642 PvuII CAG'CTG Cuts at: 2746 3322 4136 2746 Size: 576 814 6252 Fragments arranged by size: 6252 814 576 SacI G_AGCT'C Cuts at: 3932 3932 Size: 7642 SalI G'TCGA_C Cuts at: 1936 4984 1936 Size: 3048 4594 SapI GCTCTTCn'nnn_ Cuts at: 4116 4419 4116 Size: 303 7339 SbfI CC_TGCA'GG Cuts at: 4994 4994 Size: 7642 ScaI AGT'ACT Cuts at: 5890 7602 5890 Size: 1712 5930 SfcI C'TryA_G Cuts at: 826 1045 2328 2523 3023 3252 4990 6125 6806 Size: 219 1283 195 500 229 1738 1135 681 Cuts at: 6806 6997 826 Size: 191 1471 Fragments arranged by size: 1738 1471 1283 1135 681 500 229 219 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7642 SmaI CCC'GGG Cuts at: 1872 4969 1872 Size: 3097 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3224 5746 6647 6909 820 Size: 197 926 1281 2522 901 262 1553 Fragments arranged by size: 2522 1553 1281 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5433 493 Size: 4940 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7642 SphI G_CATG'C Cuts at: 1132 5000 1132 Size: 3868 3774 SspI AAT'ATT Cuts at: 5 52 5566 5 Size: 47 5514 2081 Fragments arranged by size: 5514 2081 47 StuI AGG'CCT Cuts at: 1517 4937 1517 Size: 3420 4222 StyI C'CwwG_G Cuts at: 513 1065 2147 2832 3128 3671 3814 3933 5081 Size: 552 1082 685 296 543 143 119 1148 Cuts at: 5081 513 Size: 3074 Fragments arranged by size: 3074 1148 1082 685 552 543 296 143 119 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5669 5686 5839 6024 6469 325 Size: 1255 4089 17 153 185 445 1498 Fragments arranged by size: 4089 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4605 5888 7600 Size: 274 80 33 51 1443 2628 1283 1712 Cuts at: 7600 96 Size: 138 Fragments arranged by size: 2628 1712 1443 1283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7602 7634 7602 Size: 32 7610 Tth111I GACn'n_nGTC Cuts at: 1316 3772 1316 Size: 2456 5186 XbaI T'CTAG_A Cuts at: 2299 3842 4978 2299 Size: 1543 1136 4963 Fragments arranged by size: 4963 1543 1136 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2905 2969 3931 2905 Size: 64 962 6616 Fragments arranged by size: 6616 962 64 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7642 XmaI C'CCGG_G Cuts at: 1870 4967 1870 Size: 3097 4545 XmnI GAAnn'nnTTC Cuts at: 4125 5771 4125 Size: 1646 5996 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5450 276 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BsiWI BstXI BstZ17I DraIII EcoNI FseI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI