Restriction map for
pFN21AA1594
(Linear) (Six-base) MAPSORT of: KIAA1594.seq Check: 6878 from: 1 to: 2937
KIAA1594.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AclI AA'CG_TT
Cuts at: 0 1971 2937
Size: 1971 966
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 2416 2633 2693 2937
Size: 31 2385 217 60 244
Fragments arranged by size:
2385 244 217 60 31
AhdI GACnn_n'nnGTC
Cuts at: 0 2924 2937
Size: 2924 13
AleI CACnn'nnGTG
Cuts at: 0 2908 2937
Size: 2908 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1298 1431 2937
Size: 1298 133 1506
Fragments arranged by size:
1506 1298 133
AlwNI CAG_nnn'CTG
Cuts at: 0 1017 2442 2611 2937
Size: 1017 1425 169 326
Fragments arranged by size:
1425 1017 326 169
ApoI r'AATT_y
Cuts at: 0 32 137 1133 1291 1380 1651 1866 1903
Size: 32 105 996 158 89 271 215 37
Cuts at: 1903 2937
Size: 1034
Fragments arranged by size:
1034 996 271 215 158 105 89 37
32
AvrII C'CTAG_G
Cuts at: 0 1666 2937
Size: 1666 1271
BamHI G'GATC_C
Cuts at: 0 73 823 2298 2937
Size: 73 750 1475 639
Fragments arranged by size:
1475 750 639 73
BanII G_rGCy'C
Cuts at: 0 1927 2937
Size: 1927 1010
BbsI GAAGACnn'nnnn_
Cuts at: 0 1360 2036 2512 2923 2937
Size: 1360 676 476 411 14
Fragments arranged by size:
1360 676 476 411 14
BbvCI CC'TCA_GC
Cuts at: 0 917 1984 2618 2937
Size: 917 1067 634 319
Fragments arranged by size:
1067 917 634 319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2794 2828 2937
Size: 2794 34 109
Fragments arranged by size:
2794 109 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1129 2937
Size: 1129 1808
BfrBI ATG'CAT
Cuts at: 0 2551 2937
Size: 2551 386
BglII A'GATC_T
Cuts at: 0 2855 2937
Size: 2855 82
Bme1580I G_kGCm'C
Cuts at: 0 992 2937
Size: 992 1945
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 185 2937
Size: 59 126 2752
Fragments arranged by size:
2752 126 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1278 2937
Size: 1278 1659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 690 1069 1391 1621 2342 2374 2794
Size: 152 538 379 322 230 721 32 420
Cuts at: 2794 2937
Size: 143
Fragments arranged by size:
721 538 420 379 322 230 152 143
32
Bpu10I CC'TnA_GC
Cuts at: 0 917 1984 2618 2937
Size: 917 1067 634 319
Fragments arranged by size:
1067 917 634 319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1204 2937
Size: 1204 1733
BsaI GGTCTCn'nnnn_
Cuts at: 0 1195 2937
Size: 1195 1742
BsaBI GATnn'nnATC
Cuts at: 0 2816 2937
Size: 2816 121
BsaWI w'CCGG_w
Cuts at: 0 2212 2937
Size: 2212 725
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1541 2937
Size: 1541 1396
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2824 2937
Size: 2824 113
BsiHKAI G_wGCw'C
Cuts at: 0 1638 1927 2937
Size: 1638 289 1010
Fragments arranged by size:
1638 1010 289
BsmI GAATG_Cn'
Cuts at: 0 1062 2937
Size: 1062 1875
BsmBI CGTCTCn'nnnn_
Cuts at: 0 344 2937
Size: 344 2593
Bsp1286I G_dGCh'C
Cuts at: 0 992 1638 1927 2937
Size: 992 646 289 1010
Fragments arranged by size:
1010 992 646 289
BspHI T'CATG_A
Cuts at: 0 1287 2175 2937
Size: 1287 888 762
Fragments arranged by size:
1287 888 762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1054 2937
Size: 1054 1883
BsrDI GCAATG_nn'
Cuts at: 0 1776 2937
Size: 1776 1161
BsrFI r'CCGG_y
Cuts at: 0 2666 2937
Size: 2666 271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1017 2937
Size: 1017 1920
BstBI TT'CG_AA
Cuts at: 0 36 477 2937
Size: 36 441 2460
Fragments arranged by size:
2460 441 36
BstYI r'GATC_y
Cuts at: 0 73 699 823 2298 2365 2855 2937
Size: 73 626 124 1475 67 490 82
Fragments arranged by size:
1475 626 490 124 82 73 67
Bsu36I CC'TnA_GG
Cuts at: 0 659 2937
Size: 659 2278
BtgI C'CryG_G
Cuts at: 0 1123 2937
Size: 1123 1814
ClaI AT'CG_AT
Cuts at: 0 508 2937
Size: 508 2429
EarI CTCTTCn'nnn_
Cuts at: 0 755 843 2111 2414 2937
Size: 755 88 1268 303 523
Fragments arranged by size:
1268 755 523 303 88
EcoICRI GAG'CTC
Cuts at: 0 1925 2937
Size: 1925 1012
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 152 690 1069 1391 1621 2342 2374
Size: 31 121 538 379 322 230 721 32
Cuts at: 2374 2416 2633 2693 2794 2937
Size: 42 217 60 101 143
Fragments arranged by size:
721 538 379 322 230 217 143 121
101 60 42 32 31
EcoO109I rG'GnC_Cy
Cuts at: 0 380 1671 2937
Size: 380 1291 1266
Fragments arranged by size:
1291 1266 380
EcoRI G'AATT_C
Cuts at: 0 32 137 1866 2937
Size: 32 105 1729 1071
Fragments arranged by size:
1729 1071 105 32
EcoRV GAT'ATC
Cuts at: 0 626 1188 2937
Size: 626 562 1749
Fragments arranged by size:
1749 626 562
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1588 1944 2137 2874 2937
Size: 1588 356 193 737 63
Fragments arranged by size:
1588 737 356 193 63
FspI TGC'GCA
Cuts at: 0 1823 2937
Size: 1823 1114
FspAI rTGC'GCAy
Cuts at: 0 1823 2937
Size: 1823 1114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 661 693 1469 1501 2554 2586
Size: 6 32 623 32 776 32 1053 32
Cuts at: 2586 2634 2666 2910 2937
Size: 48 32 244 27
Fragments arranged by size:
1053 776 623 244 48 32 32 32
32 32 27 6
HincII GTy'rAC
Cuts at: 0 221 2937
Size: 221 2716
HpaI GTT'AAC
Cuts at: 0 221 2937
Size: 221 2716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 2937
Size: 1795 1142
MslI CAynn'nnrTG
Cuts at: 0 2180 2908 2937
Size: 2180 728 29
Fragments arranged by size:
2180 728 29
MspA1I CmG'CkG
Cuts at: 0 741 1317 2131 2937
Size: 741 576 814 806
Fragments arranged by size:
814 806 741 576
NcoI C'CATG_G
Cuts at: 0 1123 2937
Size: 1123 1814
NdeI CA'TA_TG
Cuts at: 0 1826 2937
Size: 1826 1111
NsiI A_TGCA'T
Cuts at: 0 2553 2937
Size: 2553 384
NspI r_CATG'y
Cuts at: 0 1475 2937
Size: 1475 1462
PpuMI rG'GwC_Cy
Cuts at: 0 380 1671 2937
Size: 380 1291 1266
Fragments arranged by size:
1291 1266 380
PstI C_TGCA'G
Cuts at: 0 327 1022 2937
Size: 327 695 1915
Fragments arranged by size:
1915 695 327
PvuII CAG'CTG
Cuts at: 0 741 1317 2131 2937
Size: 741 576 814 806
Fragments arranged by size:
814 806 741 576
SacI G_AGCT'C
Cuts at: 0 1927 2937
Size: 1927 1010
SapI GCTCTTCn'nnn_
Cuts at: 0 2111 2414 2937
Size: 2111 303 523
Fragments arranged by size:
2111 523 303
SfcI C'TryA_G
Cuts at: 0 323 518 1018 1247 2937
Size: 323 195 500 229 1690
Fragments arranged by size:
1690 500 323 229 195
SmlI C'TyrA_G
Cuts at: 0 1219 2937
Size: 1219 1718
StuI AGG'CCT
Cuts at: 0 2932 2937
Size: 2932 5
StyI C'CwwG_G
Cuts at: 0 142 827 1123 1666 1809 1928 2937
Size: 142 685 296 543 143 119 1009
Fragments arranged by size:
1009 685 543 296 143 142 119
TatI w'GTAC_w
Cuts at: 0 2600 2937
Size: 2600 337
Tth111I GACn'n_nGTC
Cuts at: 0 1767 2937
Size: 1767 1170
XbaI T'CTAG_A
Cuts at: 0 294 1837 2937
Size: 294 1543 1100
Fragments arranged by size:
1543 1100 294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 900 964 1926 2937
Size: 900 64 962 1011
Fragments arranged by size:
1011 962 900 64
XmnI GAAnn'nnTTC
Cuts at: 0 2120 2937
Size: 2120 817
Enzymes that do cut:
AclI AcuI AhdI AleI AlfI AlwNI ApoI AvrII
BamHI BanII BbsI BbvCI BcgI BciVI BfrBI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaWI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BstAPI BstBI BstYI Bsu36I BtgI
ClaI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI Hin4I HincII HpaI MmeI MslI MspA1I
NcoI NdeI NsiI NspI PpuMI PstI PvuII SacI
SapI SfcI SmlI StuI StyI TatI Tth111I XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AloI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BanI BbeI BclI BglI BlpI BmgBI BmtI BsaAI
BsaHI BsaXI BseYI BsiEI BsiWI BspEI BsrBI BsrGI
BssHII BssSI BstEII BstXI BstZ17I BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoNI FseI
HaeII HindIII KasI KpnI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI XhoI
XmaI ZraI