Restriction map for pFN21AE0549
 (Circular) (Six-base) MAPSORT of: pFN21AE0549.seq  Check: 2440  from: 1  to: 5167



pFN21AE0549  5167 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:49  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2977     278 
   Size:         53      83     186     936    1441    2468

  Fragments arranged by size: 

                2468    1441     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2510    1937 
   Size:        573    4594
 
Acc65I G'GTAC_C

Cuts at:   2488    2488 
   Size:       5167
 
AclI AA'CG_TT

Cuts at:   1563    3294    3667    1563 
   Size:       1731     373    3063

  Fragments arranged by size: 

                3063    1731     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2140    2403    2445    3230    4614    4882    1632 
   Size:        508     263      42     785    1384     268    1917

  Fragments arranged by size: 

                1917    1384     785     508     268     263      42
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4970
 
AflIII A'CryG_T

Cuts at:   1203    4066    5043    1203 
   Size:       2863     977    1327

  Fragments arranged by size: 

                2863    1327     977
 
AgeI A'CCGG_T

Cuts at:   3998    3998 
   Size:       5167
 
AhdI GACnn_n'nnGTC

Cuts at:   3896    3896 
   Size:       5167
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5167
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5167
 
AlwNI CAG_nnn'CTG

Cuts at:   2910    4482    2910 
   Size:       1572    3595
 
ApaLI G'TGCA_C

Cuts at:   3224    4380    3224 
   Size:       1156    4011
 
ApoI r'AATT_y

Cuts at:   1493    2408    2476    2702    2854    4963    1493 
   Size:        915      68     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     915     226     152      68
 
AseI AT'TA_AT

Cuts at:    160    1032    3721     160 
   Size:        872    2689    1606

  Fragments arranged by size: 

                2689    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5167
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2492    1870 
   Size:         73     549    4545

  Fragments arranged by size: 

                4545     549      73
 
BamHI G'GATC_C

Cuts at:   2497    2864    2497 
   Size:        367    4800
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2488    3943     618 
   Size:        325     217     627     701    1455    1842

  Fragments arranged by size: 

                1842    1455     701     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2486    1391 
   Size:       1095    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4196
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2348    2382    3358    3392    1903 
   Size:         34     411      34     976      34    3678

  Fragments arranged by size: 

                3678     976     411      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3059    4269    3059 
   Size:       1210    3957
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5167
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    3778     136 
   Size:        107     122      71     631     605     337    1769    1525

  Fragments arranged by size: 

                1769    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2223    5162    2223 
   Size:       2939    2228
 
BlpI GC'TnA_GC

Cuts at:   2584    2584 
   Size:       5167
 
Bme1580I G_kGCm'C

Cuts at:   1790    3228    4384    1790 
   Size:       1438    1156    2573

  Fragments arranged by size: 

                2573    1438    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5167
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2898    3856     442 
   Size:        416      53      83     399      24    1481     958    1753

  Fragments arranged by size: 

                1753    1481     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5167
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3827    1239 
   Size:       2588    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2355    2355 
   Size:       5167
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3292    4157    4455    3292 
   Size:        865     298    4004

  Fragments arranged by size: 

                4004     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3830     882 
   Size:        617    2331    2219

  Fragments arranged by size: 

                2331    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2958     493 
   Size:        711    1754    2702

  Fragments arranged by size: 

                2702    1754     711
 
BsaBI GATnn'nnATC

Cuts at:   5161    5161 
   Size:       5167
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2974    3356    3991     275 
   Size:         53      83     186     936    1441     382     635    1451

  Fragments arranged by size: 

                1451    1441     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3600    3998    4272    4419    4830    3600 
   Size:        398     274     147     411    3937

  Fragments arranged by size: 

                3937     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4942

  Fragments arranged by size: 

                4942     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5167
 
BseYI C'CCAG_C

Cuts at:   2349    4370    4866    2349 
   Size:       2021     496    2650

  Fragments arranged by size: 

                2650    2021     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4594
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2617    3378    4406    1540 
   Size:        275     186     616     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     616     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2361    3228    3313    4384    2361 
   Size:        867      85    1071    3144

  Fragments arranged by size: 

                3144    1071     867      85
 
BsmI GAATG_Cn'

Cuts at:   2425    2678    2771    2425 
   Size:        253      93    4821

  Fragments arranged by size: 

                4821     253      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2361    2486    3228    3313    4384    1391 
   Size:        399     571     125     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     571     399     125      85
 
BspEI T'CCGG_A

Cuts at:   4830    4830 
   Size:       5167
 
BspHI T'CATG_A

Cuts at:   3056    4786    3056 
   Size:       1730    3437
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2245    2522     844 
   Size:       1401     277    3489

  Fragments arranged by size: 

                3489    1401     277
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3054    1394 
   Size:        429    1231    3507

  Fragments arranged by size: 

                3507    1231     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3662    3836      65 
   Size:       1183    2414     174    1396

  Fragments arranged by size: 

                2414    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3811    3998    1700 
   Size:        110    2001     187    2869

  Fragments arranged by size: 

                2869    2001     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5167
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5167
 
BssSI C'ACGA_G

Cuts at:   3227    4239    3227 
   Size:       1012    4155
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5167
 
BstBI TT'CG_AA

Cuts at:   3971    3971 
   Size:       5167
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5167
 
BstYI r'GATC_y

Cuts at:   1972    2223    2497    2864    3249    3266    4718    4804    4816 
   Size:        251     274     367     385      17    1452      86      12

Cuts at:   4816    5162    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     274     251      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3676

  Fragments arranged by size: 

                3676     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4428

  Fragments arranged by size: 

                4428     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2683    3477    3497    2683 
   Size:        794      20    4353

  Fragments arranged by size: 

                4353     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5167
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5132
 
DraI TTT'AAA

Cuts at:   2215    2471    2215 
   Size:        256    4911
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4174     809 
   Size:        507    2858    1802

  Fragments arranged by size: 

                2858    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2010    2614    3503 
   Size:         54    1425      50     275      18     180     604     889

Cuts at:   3503    5132       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     604     275     180      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2614    1537 
   Size:        275     802    4090

  Fragments arranged by size: 

                4090     802     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2421    3097    1353 
   Size:       1068     676    3423

  Fragments arranged by size: 

                3423    1068     676
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3754    4128    4274     387 
   Size:       1064     138    2165     374     146    1280

  Fragments arranged by size: 

                2165    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2140    2403    2445    3230    3827    4614    4882 
   Size:        393     508     263      42     785     597     787     268

Cuts at:   4882    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     787     785     597     508     393     268     263
                  42
 
EcoRI G'AATT_C

Cuts at:   2476    2854    2476 
   Size:        378    4789
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2170    2170 
   Size:       5167
 
FspI TGC'GCA

Cuts at:   3673    3673 
   Size:       5167
 
HaeII r_GCGC'y

Cuts at:   1678    4314    1678 
   Size:       2636    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3808    3840    3882    3914    1373 
   Size:         32     135      32    2236      32      42      32    2626

  Fragments arranged by size: 

                2626    2236     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2511    2757     669 
   Size:        988     281     573     246    3079

  Fragments arranged by size: 

                3079     988     573     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5167
 
HpaI GTT'AAC

Cuts at:   2757    2757 
   Size:       5167
 
KpnI G_GTAC'C

Cuts at:   2492    2492 
   Size:       5167
 
MfeI C'AATT_G

Cuts at:   2766    2766 
   Size:       5167
 
MluI A'CGCG_T

Cuts at:   5043    5043 
   Size:       5167
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4281    4465    1305 
   Size:       2976     184    2007

  Fragments arranged by size: 

                2976    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3345

  Fragments arranged by size: 

                3345    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3125    3484    3643     518 
   Size:        648    1959     359     159    2042

  Fragments arranged by size: 

                2042    1959     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2583    3260    4408    4653    1606 
   Size:        977     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     977     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3676

  Fragments arranged by size: 

                3676     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2140     387 
   Size:       1753    3414
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5167
 
NotI GC'GGCC_GC

Cuts at:   2614    2614 
   Size:       5167
 
NspI r_CATG'y

Cuts at:   1132    1229    2525    4070    1132 
   Size:         97    1296    1545    2229

  Fragments arranged by size: 

                2229    1545    1296      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5167
 
PciI A'CATG_T

Cuts at:   4066    4066 
   Size:       5167
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5167
 
PmeI GTTT'AAAC

Cuts at:   2471    2471 
   Size:       5167
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3236    3268    4749    4781    3236 
   Size:         32    1481      32    3622

  Fragments arranged by size: 

                3622    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5167
 
PsiI TTA'TAA

Cuts at:   2737    2737 
   Size:       5167
 
PstI C_TGCA'G

Cuts at:    830    2519     830 
   Size:       1689    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5167
 
SalI G'TCGA_C

Cuts at:   1936    2509    1936 
   Size:        573    4594
 
SbfI CC_TGCA'GG

Cuts at:   2519    2519 
   Size:       5167
 
ScaI AGT'ACT

Cuts at:   3415    5127    3415 
   Size:       1712    3455
 
SfcI C'TryA_G

Cuts at:    826    1045    2515    3650    4331    4522     826 
   Size:        219    1470    1135     681     191    1471

  Fragments arranged by size: 

                1471    1470    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5167
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5167
 
SmaI CCC'GGG

Cuts at:   1872    2494    1872 
   Size:        622    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3271    4172    4434     820 
   Size:        197     926    1328     901     262    1553

  Fragments arranged by size: 

                1553    1328     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2958     493 
   Size:       2465    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5167
 
SphI G_CATG'C

Cuts at:   1132    2525    1132 
   Size:       1393    3774
 
SspI AAT'ATT

Cuts at:      5      52    3091       5 
   Size:         47    3039    2081

  Fragments arranged by size: 

                3039    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5167
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2606     513 
   Size:        552     939     602    3074

  Fragments arranged by size: 

                3074     939     602     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2247    3194    3211    3364    3549    3994     325 
   Size:       1255     667     947      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     947     667     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3413    5125      96 
   Size:        274      80      33      51    1443    1436    1712     138

  Fragments arranged by size: 

                1712    1443    1436     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5127    5159    5127 
   Size:         32    5135
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5167
 
XbaI T'CTAG_A

Cuts at:   2503    2503 
   Size:       5167
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5167
 
XmaI C'CCGG_G

Cuts at:   1870    2492    1870 
   Size:        622    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2066    3296    2066 
   Size:       1230    3937
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2975     276 
   Size:         53      83     186     936    1441    2468

  Fragments arranged by size: 

                2468    1441     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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