(Circular) (Six-base) MAPSORT of: pFN21AB7660.seq Check: 214 from: 1 to: 5890 pFN21AB7660 5890 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2755 2755 Size: 5890 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2718 3233 1937 Size: 781 515 4594 Fragments arranged by size: 4594 781 515 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3056 3953 5337 5605 1632 Size: 1424 897 1384 268 1917 Fragments arranged by size: 1917 1424 1384 897 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 2276 4789 5766 1203 Size: 1073 2513 977 1327 Fragments arranged by size: 2513 1327 1073 977 AgeI A'CCGG_T Cuts at: 2603 4721 2603 Size: 2118 3772 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 2597 3633 5205 2597 Size: 1036 1572 3282 Fragments arranged by size: 3282 1572 1036 ApaI G_GGCC'C Cuts at: 2069 2121 2206 2447 3165 3183 2069 Size: 52 85 241 718 18 4776 Fragments arranged by size: 4776 718 241 85 52 18 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 2337 3199 3425 3577 5686 1493 Size: 844 862 226 152 2109 1697 Fragments arranged by size: 2109 1697 862 844 226 152 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 2625 3142 3215 1870 Size: 73 682 517 73 4545 Fragments arranged by size: 4545 682 517 73 73 BamHI G'GATC_C Cuts at: 2089 3220 3587 2089 Size: 1131 367 4392 Fragments arranged by size: 4392 1131 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2171 2357 2411 2527 2767 Size: 325 217 627 384 186 54 116 240 Cuts at: 2767 3211 4666 618 Size: 444 1455 1842 Fragments arranged by size: 1842 1455 627 444 384 325 240 217 186 116 54 BanII G_rGCy'C Cuts at: 1391 2069 2078 2121 2148 2206 2310 2447 2678 Size: 678 9 43 27 58 104 137 231 Cuts at: 2678 3165 3183 3209 1391 Size: 487 18 26 4072 Fragments arranged by size: 4072 678 487 231 137 104 58 43 27 26 18 9 BbeI G_GCGC'C Cuts at: 2361 2415 2361 Size: 54 5836 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4919 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2952 3178 4501 Size: 107 122 71 631 605 1280 226 1323 Cuts at: 4501 136 Size: 1525 Fragments arranged by size: 1525 1323 1280 631 605 226 122 107 71 BglII A'GATC_T Cuts at: 2369 5885 2369 Size: 3516 2374 BlpI GC'TnA_GC Cuts at: 3129 3307 3129 Size: 178 5712 Bme1580I G_kGCm'C Cuts at: 1790 2069 2121 2174 2206 2447 2532 2621 2772 Size: 279 52 53 32 241 85 89 151 Cuts at: 2772 3165 3183 3951 5107 1790 Size: 393 18 768 1156 2573 Fragments arranged by size: 2573 1156 768 393 279 241 151 89 85 53 52 32 18 BmgBI CAC'GTC Cuts at: 1318 2277 1318 Size: 959 4931 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2514 2522 3621 Size: 416 53 83 399 24 1097 8 1099 Cuts at: 3621 4579 442 Size: 958 1753 Fragments arranged by size: 1753 1099 1097 958 416 399 83 53 24 8 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2351 2733 2826 3098 4550 1239 Size: 1085 27 382 93 272 1452 2579 Fragments arranged by size: 2579 1452 1085 382 272 93 27 Bpu10I CC'TnA_GC Cuts at: 2677 2677 Size: 5890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4015 4880 5178 4015 Size: 865 298 4727 Fragments arranged by size: 4727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2292 2594 4553 882 Size: 617 793 302 1959 2219 Fragments arranged by size: 2219 1959 793 617 302 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 2412 3697 4079 Size: 53 83 186 936 825 54 1285 382 Cuts at: 4079 4714 275 Size: 635 1451 Fragments arranged by size: 1451 1285 936 825 635 382 186 83 54 53 BsaWI w'CCGG_w Cuts at: 2603 2939 4323 4721 4995 5142 5553 2603 Size: 336 1384 398 274 147 411 2940 Fragments arranged by size: 2940 1384 411 398 336 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5665 Fragments arranged by size: 5665 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2560 3035 3130 1186 Size: 1374 475 95 3946 Fragments arranged by size: 3946 1374 475 95 BseYI C'CCAG_C Cuts at: 2147 2531 2577 2669 2789 2797 5093 5589 2147 Size: 384 46 92 120 8 2296 496 2448 Fragments arranged by size: 2448 2296 496 384 120 92 46 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5317 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2269 3340 4101 5129 1540 Size: 275 186 268 1071 761 1028 2301 Fragments arranged by size: 2301 1071 1028 761 275 268 186 BsiHKAI G_wGCw'C Cuts at: 2749 3951 4036 5107 2749 Size: 1202 85 1071 3532 Fragments arranged by size: 3532 1202 1071 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2078 2121 2148 2174 2206 2310 Size: 399 279 9 43 27 26 32 104 Cuts at: 2310 2447 2532 2621 2678 2749 2772 3165 3183 Size: 137 85 89 57 71 23 393 18 Cuts at: 3183 3209 3951 4036 5107 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 279 137 104 89 85 85 71 57 43 32 27 26 26 23 18 9 BspEI T'CCGG_A Cuts at: 2939 5553 2939 Size: 2614 3276 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2755 3245 844 Size: 1911 490 3489 Fragments arranged by size: 3489 1911 490 BsrBI CCG'CTC Cuts at: 1394 1823 2010 2496 3064 3777 1394 Size: 429 187 486 568 713 3507 Fragments arranged by size: 3507 713 568 486 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 4385 4559 65 Size: 1183 3137 174 1396 Fragments arranged by size: 3137 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2603 4534 4721 1700 Size: 110 793 1931 187 2869 Fragments arranged by size: 2869 1931 793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5890 BssHII G'CGCG_C Cuts at: 1925 3100 1925 Size: 1175 4715 BssSI C'ACGA_G Cuts at: 3950 4962 3950 Size: 1012 4878 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5890 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 2909 1184 Size: 1725 4165 BstXI CCAn_nnnn'nTGG Cuts at: 2986 2986 Size: 5890 BstYI r'GATC_y Cuts at: 1972 2089 2369 3045 3220 3587 3972 3989 5441 Size: 117 280 676 175 367 385 17 1452 Cuts at: 5441 5527 5539 5885 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 676 385 367 346 280 175 117 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2350 2487 2350 Size: 137 5753 BtgI C'CryG_G Cuts at: 513 1065 2052 2239 2263 2979 3152 513 Size: 552 987 187 24 716 173 3251 Fragments arranged by size: 3251 987 716 552 187 173 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5151 Fragments arranged by size: 5151 683 56 BtsI GCAGTG_nn' Cuts at: 3406 4200 4220 3406 Size: 794 20 5076 Fragments arranged by size: 5076 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2394 2898 3108 Size: 54 1425 50 275 18 564 504 210 Cuts at: 3108 3337 4226 5855 8 Size: 229 889 1629 43 Fragments arranged by size: 1629 1425 889 564 504 275 229 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3337 1537 Size: 275 1525 4090 Fragments arranged by size: 4090 1525 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2748 3820 1353 Size: 665 730 1072 3423 Fragments arranged by size: 3423 1072 730 665 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2337 3074 4477 4851 4997 387 Size: 1064 138 748 737 1403 374 146 1280 Fragments arranged by size: 1403 1280 1064 748 737 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2351 2733 2826 3056 3098 3953 Size: 393 692 27 382 93 230 42 855 Cuts at: 3953 4550 5337 5605 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 855 787 692 597 393 382 268 230 93 42 27 EcoNI CCTnn'n_nnAGG Cuts at: 2438 2650 2438 Size: 212 5678 EcoO109I rG'GnC_Cy Cuts at: 2065 2066 2118 2202 2203 2346 2443 2444 2688 Size: 1 52 84 1 143 97 1 244 Cuts at: 2688 3148 3162 3180 2065 Size: 460 14 18 4775 Fragments arranged by size: 4775 460 244 143 97 84 52 18 14 1 1 1 EcoRI G'AATT_C Cuts at: 2337 3199 3577 2337 Size: 862 378 4650 Fragments arranged by size: 4650 862 378 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 2361 2415 2587 5037 1678 Size: 683 54 172 2450 2531 Fragments arranged by size: 2531 2450 683 172 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2921 2953 4531 4563 4605 Size: 32 135 32 1349 32 1578 32 42 Cuts at: 4605 4637 1373 Size: 32 2626 Fragments arranged by size: 2626 1578 1349 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3234 3480 669 Size: 988 281 1296 246 3079 Fragments arranged by size: 3079 1296 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5890 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KasI G'GCGC_C Cuts at: 2357 2411 2357 Size: 54 5836 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2367 5004 5188 1305 Size: 1062 2637 184 2007 Fragments arranged by size: 2637 2007 1062 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 2900 3110 10 Size: 54 1425 343 564 504 210 2790 Fragments arranged by size: 2790 1425 564 504 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 3848 4207 4366 518 Size: 648 2682 359 159 2042 Fragments arranged by size: 2682 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2265 2521 3124 3133 3306 3983 5131 5376 Size: 659 256 603 9 173 677 1148 245 Cuts at: 5376 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 659 603 256 245 173 9 NarI GG'CG_CC Cuts at: 2358 2412 2358 Size: 54 5836 NcoI C'CATG_G Cuts at: 513 1065 2979 513 Size: 552 1914 3424 Fragments arranged by size: 3424 1914 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 2725 3248 4793 1132 Size: 97 1496 523 1545 2229 Fragments arranged by size: 2229 1545 1496 523 97 PasI CC'CwG_GG Cuts at: 1348 2069 2078 1348 Size: 721 9 5160 Fragments arranged by size: 5160 721 9 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2198 2245 2454 2524 3158 1108 Size: 1090 47 209 70 634 3840 Fragments arranged by size: 3840 1090 634 209 70 47 PfoI T'CCnGG_A Cuts at: 2328 2479 2702 3015 3048 2328 Size: 151 223 313 33 5170 Fragments arranged by size: 5170 313 223 151 33 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2688 3148 2688 Size: 460 5430 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PspOMI G'GGCC_C Cuts at: 2065 2117 2202 2443 3161 3179 2065 Size: 52 85 241 718 18 4776 Fragments arranged by size: 4776 718 241 85 52 18 PstI C_TGCA'G Cuts at: 830 3242 830 Size: 2412 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 2521 3124 3133 2521 Size: 603 9 5278 Fragments arranged by size: 5278 603 9 RsrII CG'GwC_CG Cuts at: 3013 3013 Size: 5890 SacII CC_GC'GG Cuts at: 2266 2266 Size: 5890 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SanDI GG'GwC_CC Cuts at: 3148 3148 Size: 5890 SbfI CC_TGCA'GG Cuts at: 3242 3242 Size: 5890 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 2715 3238 4373 5054 5245 826 Size: 219 1670 523 1135 681 191 1471 Fragments arranged by size: 1670 1471 1135 681 523 219 191 SfoI GGC'GCC Cuts at: 2359 2413 2359 Size: 54 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 2627 3217 1872 Size: 755 590 4545 Fragments arranged by size: 4545 755 590 SmlI C'TyrA_G Cuts at: 820 1017 1943 3994 4895 5157 820 Size: 197 926 2051 901 262 1553 Fragments arranged by size: 2051 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 2433 1517 Size: 916 4974 StyI C'CwwG_G Cuts at: 513 1065 2541 2979 3329 513 Size: 552 1476 438 350 3074 Fragments arranged by size: 3074 1476 552 438 350 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2732 2828 3917 3934 4087 4272 4717 Size: 1255 1152 96 1089 17 153 185 445 Cuts at: 4717 325 Size: 1498 Fragments arranged by size: 1498 1255 1152 1089 445 185 153 96 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4136 5848 96 Size: 274 80 33 51 1443 2159 1712 138 Fragments arranged by size: 2159 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5850 5882 5850 Size: 32 5858 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2404 2389 Size: 15 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 2625 3215 1870 Size: 755 590 4545 Fragments arranged by size: 4545 755 590 XmnI GAAnn'nnTTC Cuts at: 4019 4019 Size: 5890 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SapI SexAI SfiI SrfI SwaI