(Circular) (Six-base) MAPSORT of: pFN21AE2418.seq Check: 1908 from: 1 to: 7222 pFN21AE2418 7222 bp With 182 enzymes: SgfI * October 12, 2018 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2072 3182 3914 4177 2072 Size: 1110 732 263 5117 Fragments arranged by size: 5117 1110 732 263 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2688 5032 278 Size: 53 83 186 936 1152 2344 2468 Fragments arranged by size: 2468 2344 1152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3327 3462 3753 4565 1937 Size: 1390 135 291 812 4594 Fragments arranged by size: 4594 1390 812 291 135 Acc65I G'GTAC_C Cuts at: 4543 4543 Size: 7222 AclI AA'CG_TT Cuts at: 1563 5349 5722 1563 Size: 3786 373 3063 Fragments arranged by size: 3786 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2585 2996 3580 4216 4490 5285 6669 6937 Size: 953 411 584 636 274 795 1384 268 Cuts at: 6937 1632 Size: 1917 Fragments arranged by size: 1917 1384 953 795 636 584 411 274 268 AflII C'TTAA_G Cuts at: 820 1017 4145 820 Size: 197 3128 3897 Fragments arranged by size: 3897 3128 197 AflIII A'CryG_T Cuts at: 1203 6121 7098 1203 Size: 4918 977 1327 Fragments arranged by size: 4918 1327 977 AgeI A'CCGG_T Cuts at: 2279 4221 6053 2279 Size: 1942 1832 3448 Fragments arranged by size: 3448 1942 1832 AhdI GACnn_n'nnGTC Cuts at: 5951 5951 Size: 7222 AleI CACnn'nnGTG Cuts at: 1166 3584 3785 4271 1166 Size: 2418 201 486 4117 Fragments arranged by size: 4117 2418 486 201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2882 4199 1184 Size: 1698 1317 4207 Fragments arranged by size: 4207 1698 1317 AlwNI CAG_nnn'CTG Cuts at: 2392 3565 4365 4965 6537 2392 Size: 1173 800 600 1572 3077 Fragments arranged by size: 3077 1572 1173 800 600 ApaI G_GGCC'C Cuts at: 3135 3135 Size: 7222 ApaLI G'TGCA_C Cuts at: 2776 3137 5279 6435 2776 Size: 361 2142 1156 3563 Fragments arranged by size: 3563 2142 1156 361 ApoI r'AATT_y Cuts at: 1493 4531 4757 4909 7018 1493 Size: 3038 226 152 2109 1697 Fragments arranged by size: 3038 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5776 160 Size: 872 4744 1606 Fragments arranged by size: 4744 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 AvaI C'yCGr_G Cuts at: 1870 1943 3086 4294 4410 4433 4547 1870 Size: 73 1143 1208 116 23 114 4545 Fragments arranged by size: 4545 1208 1143 116 114 73 23 BamHI G'GATC_C Cuts at: 4552 4919 4552 Size: 367 6855 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 3096 4543 5998 618 Size: 325 217 627 495 814 1447 1455 1842 Fragments arranged by size: 1842 1455 1447 814 627 495 325 217 BanII G_rGCy'C Cuts at: 1391 2991 3087 3135 4541 1391 Size: 1600 96 48 1406 4072 Fragments arranged by size: 4072 1600 1406 96 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2333 2477 3521 3754 3810 3973 4296 Size: 971 434 144 1044 233 56 163 323 Cuts at: 4296 4483 928 Size: 187 3667 Fragments arranged by size: 3667 1044 971 434 323 233 187 163 144 56 BbvCI CC'TCA_GC Cuts at: 4485 4485 Size: 7222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5413 5447 1903 Size: 34 3476 34 3678 Fragments arranged by size: 3678 3476 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5114 6324 5114 Size: 1210 6012 BclI T'GATC_A Cuts at: 1548 3342 1548 Size: 1794 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5833 136 Size: 107 122 71 631 605 4161 1525 Fragments arranged by size: 4161 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7217 7217 Size: 7222 BlpI GC'TnA_GC Cuts at: 4428 4639 4428 Size: 211 7011 Bme1580I G_kGCm'C Cuts at: 1790 2780 3135 3141 5283 6439 1790 Size: 990 355 6 2142 1156 2573 Fragments arranged by size: 2573 2142 1156 990 355 6 BmgBI CAC'GTC Cuts at: 1318 3840 1318 Size: 2522 4700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4953 5911 442 Size: 416 53 83 399 24 3536 958 1753 Fragments arranged by size: 3536 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7222 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2233 3202 2233 Size: 969 6253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 2091 2414 2558 3002 3050 3215 3453 Size: 841 11 323 144 444 48 165 238 Cuts at: 3453 3932 3968 5882 1239 Size: 479 36 1914 2579 Fragments arranged by size: 2579 1914 841 479 444 323 238 165 144 48 36 11 Bpu10I CC'TnA_GC Cuts at: 4171 4325 4485 4171 Size: 154 160 6908 Fragments arranged by size: 6908 160 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3590 5347 6212 6510 3590 Size: 1757 865 298 4302 Fragments arranged by size: 4302 1757 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2079 3215 3723 4470 5885 882 Size: 617 580 1136 508 747 1415 2219 Fragments arranged by size: 2219 1415 1136 747 617 580 508 BsaAI yAC'GTr Cuts at: 493 1204 5013 493 Size: 711 3809 2702 Fragments arranged by size: 3809 2702 711 BsaBI GATnn'nnATC Cuts at: 2945 3692 7216 2945 Size: 747 3524 2951 Fragments arranged by size: 3524 2951 747 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2685 3011 4311 5029 Size: 53 83 186 936 1152 326 1300 718 Cuts at: 5029 5411 6046 275 Size: 382 635 1451 Fragments arranged by size: 1451 1300 1152 936 718 635 382 326 186 83 53 BsaWI w'CCGG_w Cuts at: 2279 4221 5655 6053 6327 6474 6885 2279 Size: 1942 1434 398 274 147 411 2616 Fragments arranged by size: 2616 1942 1434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 2670 2700 3074 Size: 30 165 30 675 30 562 30 374 Cuts at: 3074 3104 1178 Size: 30 5296 Fragments arranged by size: 5296 675 562 374 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2999 3133 3471 1186 Size: 1000 813 134 338 4937 Fragments arranged by size: 4937 1000 813 338 134 BseYI C'CCAG_C Cuts at: 2118 2788 3029 6425 6921 2118 Size: 670 241 3396 496 2419 Fragments arranged by size: 3396 2419 670 496 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2606 2855 2948 3112 1163 Size: 573 870 249 93 164 5273 Fragments arranged by size: 5273 870 573 249 164 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2832 2904 3506 4672 5433 6461 Size: 275 186 831 72 602 1166 761 1028 Cuts at: 6461 1540 Size: 2301 Fragments arranged by size: 2301 1166 1028 831 761 602 275 186 72 BsiHKAI G_wGCw'C Cuts at: 2780 2991 3141 4411 5283 5368 6439 2780 Size: 211 150 1270 872 85 1071 3563 Fragments arranged by size: 3563 1270 1071 872 211 150 85 BsmI GAATG_Cn' Cuts at: 4733 4826 4733 Size: 93 7129 BsmBI CGTCTCn'nnnn_ Cuts at: 2262 2632 2262 Size: 370 6852 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2780 2991 3087 3135 3141 4411 4541 Size: 399 990 211 96 48 6 1270 130 Cuts at: 4541 5283 5368 6439 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1270 1071 990 742 399 211 130 96 85 48 6 BspEI T'CCGG_A Cuts at: 6885 6885 Size: 7222 BspHI T'CATG_A Cuts at: 3705 5111 6841 3705 Size: 1406 1730 4086 Fragments arranged by size: 4086 1730 1406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2072 2508 3182 3914 4177 4577 844 Size: 1228 436 674 732 263 400 3489 Fragments arranged by size: 3489 1228 732 674 436 400 263 BsrBI CCG'CTC Cuts at: 1394 1823 4170 5109 1394 Size: 429 2347 939 3507 Fragments arranged by size: 3507 2347 939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5717 5891 65 Size: 1183 4469 174 1396 Fragments arranged by size: 4469 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2279 4221 5866 6053 1700 Size: 110 469 1942 1645 187 2869 Fragments arranged by size: 2869 1942 1645 469 187 110 BsrGI T'GTAC_A Cuts at: 96 2931 3336 96 Size: 2835 405 3982 Fragments arranged by size: 3982 2835 405 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7222 BssSI C'ACGA_G Cuts at: 2198 5282 6294 2198 Size: 3084 1012 3126 Fragments arranged by size: 3126 3084 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7222 BstBI TT'CG_AA Cuts at: 6026 6026 Size: 7222 BstEII G'GTnAC_C Cuts at: 1184 2656 1184 Size: 1472 5750 BstXI CCAn_nnnn'nTGG Cuts at: 2788 3787 3910 3937 2788 Size: 999 123 27 6073 Fragments arranged by size: 6073 999 123 27 BstYI r'GATC_y Cuts at: 1972 2389 3075 3438 4552 4919 5304 5321 6773 Size: 417 686 363 1114 367 385 17 1452 Cuts at: 6773 6859 6871 7217 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1114 686 417 385 367 363 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2333 2333 Size: 7222 BtgI C'CryG_G Cuts at: 513 1065 2127 2701 3354 3375 3582 3624 3673 Size: 552 1062 574 653 21 207 42 49 Cuts at: 3673 513 Size: 4062 Fragments arranged by size: 4062 1062 653 574 552 207 49 42 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2611 4027 487 Size: 683 56 1385 1416 3682 Fragments arranged by size: 3682 1416 1385 683 56 BtsI GCAGTG_nn' Cuts at: 2618 4738 5532 5552 2618 Size: 2120 794 20 4288 Fragments arranged by size: 4288 2120 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7222 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7187 DraI TTT'AAA Cuts at: 4526 4526 Size: 7222 DraIII CAC_nnn'GTG Cuts at: 3120 3120 Size: 7222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3293 4487 6229 809 Size: 507 1977 1194 1742 1802 Fragments arranged by size: 1977 1802 1742 1194 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2769 2829 Size: 54 1425 50 275 18 300 639 60 Cuts at: 2829 2889 2901 3503 3576 4669 5558 7187 8 Size: 60 12 602 73 1093 889 1629 43 Fragments arranged by size: 1629 1425 1093 889 639 602 300 275 73 60 60 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2829 2901 3503 4669 1537 Size: 275 1017 72 602 1166 4090 Fragments arranged by size: 4090 1166 1017 602 275 72 EarI CTCTTCn'nnn_ Cuts at: 1353 2414 4130 5152 1353 Size: 1061 1716 1022 3423 Fragments arranged by size: 3423 1716 1061 1022 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5809 6183 6329 387 Size: 1064 138 4220 374 146 1280 Fragments arranged by size: 4220 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2989 2989 Size: 7222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2091 2414 2558 2585 2996 3002 Size: 393 448 11 323 144 27 411 6 Cuts at: 3002 3050 3215 3453 3580 3932 3968 4216 4490 Size: 48 165 238 127 352 36 248 274 Cuts at: 4490 5285 5882 6669 6937 1239 Size: 795 597 787 268 1524 Fragments arranged by size: 1524 795 787 597 448 411 393 352 323 274 268 248 238 165 144 127 48 36 27 11 6 EcoNI CCTnn'n_nnAGG Cuts at: 2463 2882 3367 2463 Size: 419 485 6318 Fragments arranged by size: 6318 485 419 EcoO109I rG'GnC_Cy Cuts at: 3857 3870 3857 Size: 13 7209 EcoRI G'AATT_C Cuts at: 4531 4909 4531 Size: 378 6844 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3322 3997 3322 Size: 675 6547 FspI TGC'GCA Cuts at: 5728 5728 Size: 7222 HaeII r_GCGC'y Cuts at: 1678 2844 6369 1678 Size: 1166 3525 2531 Fragments arranged by size: 3525 2531 1166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2670 2702 3214 Size: 32 135 32 506 32 560 32 512 Cuts at: 3214 3246 3428 3460 3824 3856 4406 4438 5863 Size: 32 182 32 364 32 550 32 1425 Cuts at: 5863 5895 5937 5969 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1425 560 550 512 506 364 182 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2578 4066 4566 4812 669 Size: 988 281 640 1488 500 246 3079 Fragments arranged by size: 3079 1488 988 640 500 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7222 HpaI GTT'AAC Cuts at: 4812 4812 Size: 7222 KpnI G_GTAC'C Cuts at: 4547 4547 Size: 7222 MfeI C'AATT_G Cuts at: 4821 4821 Size: 7222 MluI A'CGCG_T Cuts at: 7098 7098 Size: 7222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6336 6520 1305 Size: 5031 184 2007 Fragments arranged by size: 5031 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3578 10 Size: 54 1425 343 1746 3654 Fragments arranged by size: 3654 1746 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3584 3785 3935 4271 5180 5539 5698 Size: 648 2418 201 150 336 909 359 159 Cuts at: 5698 518 Size: 2042 Fragments arranged by size: 2418 2042 909 648 359 336 201 159 150 MspA1I CmG'CkG Cuts at: 1606 2039 2788 3016 3029 3070 3259 3675 3785 Size: 433 749 228 13 41 189 416 110 Cuts at: 3785 4638 5315 6463 6708 1606 Size: 853 677 1148 245 2120 Fragments arranged by size: 2120 1148 853 749 677 433 416 245 228 189 110 41 13 NcoI C'CATG_G Cuts at: 513 1065 2701 3354 3624 513 Size: 552 1636 653 270 4111 Fragments arranged by size: 4111 1636 653 552 270 NdeI CA'TA_TG Cuts at: 387 387 Size: 7222 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7222 NotI GC'GGCC_GC Cuts at: 4669 4669 Size: 7222 NspI r_CATG'y Cuts at: 1132 1229 2932 3244 3661 4039 4580 6125 1132 Size: 97 1703 312 417 378 541 1545 2229 Fragments arranged by size: 2229 1703 1545 541 417 378 312 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7222 PciI A'CATG_T Cuts at: 6121 6121 Size: 7222 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 3688 1108 Size: 1284 1296 4642 Fragments arranged by size: 4642 1296 1284 PfoI T'CCnGG_A Cuts at: 2535 3078 3104 4122 2535 Size: 543 26 1018 5635 Fragments arranged by size: 5635 1018 543 26 PmeI GTTT'AAAC Cuts at: 4526 4526 Size: 7222 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5291 5323 6804 6836 5291 Size: 32 1481 32 5677 Fragments arranged by size: 5677 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7222 PsiI TTA'TAA Cuts at: 4792 4792 Size: 7222 PspOMI G'GGCC_C Cuts at: 3131 3131 Size: 7222 PstI C_TGCA'G Cuts at: 830 2418 2814 3276 3498 3561 3869 4107 4188 Size: 1588 396 462 222 63 308 238 81 Cuts at: 4188 4574 830 Size: 386 3478 Fragments arranged by size: 3478 1588 462 396 386 308 238 222 81 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7222 PvuII CAG'CTG Cuts at: 2039 2788 3029 2039 Size: 749 241 6232 Fragments arranged by size: 6232 749 241 SacI G_AGCT'C Cuts at: 2991 2991 Size: 7222 SacII CC_GC'GG Cuts at: 3676 3676 Size: 7222 SalI G'TCGA_C Cuts at: 1936 4564 1936 Size: 2628 4594 SapI GCTCTTCn'nnn_ Cuts at: 2414 4130 2414 Size: 1716 5506 SbfI CC_TGCA'GG Cuts at: 3276 3869 4574 3276 Size: 593 705 5924 Fragments arranged by size: 5924 705 593 ScaI AGT'ACT Cuts at: 5470 7182 5470 Size: 1712 5510 SfcI C'TryA_G Cuts at: 826 1045 2414 2810 3208 3272 3367 3494 3535 Size: 219 1369 396 398 64 95 127 41 Cuts at: 3535 3557 3865 4103 4184 4570 5705 6386 6577 Size: 22 308 238 81 386 1135 681 191 Cuts at: 6577 826 Size: 1471 Fragments arranged by size: 1471 1369 1135 681 398 396 386 308 238 219 191 127 95 81 64 41 22 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 SgrAI Cr'CCGG_yG Cuts at: 1700 2279 1700 Size: 579 6643 SmaI CCC'GGG Cuts at: 1872 4296 4549 1872 Size: 2424 253 4545 Fragments arranged by size: 4545 2424 253 SmlI C'TyrA_G Cuts at: 820 1017 1943 3605 4145 4410 5326 6227 6489 Size: 197 926 1662 540 265 916 901 262 Cuts at: 6489 820 Size: 1553 Fragments arranged by size: 1662 1553 926 916 901 540 265 262 197 SnaBI TAC'GTA Cuts at: 493 5013 493 Size: 4520 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7222 SphI G_CATG'C Cuts at: 1132 4580 1132 Size: 3448 3774 SspI AAT'ATT Cuts at: 5 52 5146 5 Size: 47 5094 2081 Fragments arranged by size: 5094 2081 47 StuI AGG'CCT Cuts at: 1517 4163 1517 Size: 2646 4576 StyI C'CwwG_G Cuts at: 513 1065 2701 3354 3624 3642 4661 513 Size: 552 1636 653 270 18 1019 3074 Fragments arranged by size: 3074 1636 1019 653 552 270 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4043 5249 5266 5419 5604 6049 325 Size: 1255 2463 1206 17 153 185 445 1498 Fragments arranged by size: 2463 1498 1255 1206 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2931 3336 3591 Size: 274 80 33 51 1443 954 405 255 Cuts at: 3591 3897 5468 7180 96 Size: 306 1571 1712 138 Fragments arranged by size: 1712 1571 1443 954 405 306 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3074 3106 7182 7214 3074 Size: 32 4076 32 3082 Fragments arranged by size: 4076 3082 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2306 1316 Size: 990 6232 XbaI T'CTAG_A Cuts at: 4558 4558 Size: 7222 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2851 4120 2851 Size: 1269 5953 XhoI C'TCGA_G Cuts at: 1943 4410 1943 Size: 2467 4755 XmaI C'CCGG_G Cuts at: 1870 4294 4547 1870 Size: 2424 253 4545 Fragments arranged by size: 4545 2424 253 XmnI GAAnn'nnTTC Cuts at: 4139 4163 5351 4139 Size: 24 1188 6010 Fragments arranged by size: 6010 1188 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2686 5030 276 Size: 53 83 186 936 1152 2344 2468 Fragments arranged by size: 2468 2344 1152 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI