Restriction map for
pFN21AE2418
(Linear) (Six-base) MAPSORT of: KIEE2418.seq Check: 7845 from: 1 to: 2517
KIEE2418.orf
With 182 enzymes: SgfI *
October 12, 2018 16:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 67 1177 1909 2172 2517
Size: 67 1110 732 263 345
Fragments arranged by size:
1110 732 345 263 67
AatII G_ACGT'C
Cuts at: 0 683 2517
Size: 683 1834
AccI GT'mk_AC
Cuts at: 0 1322 1457 1748 2517
Size: 1322 135 291 769
Fragments arranged by size:
1322 769 291 135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 991 1575 2211 2485 2517
Size: 580 411 584 636 274 32
Fragments arranged by size:
636 584 580 411 274 32
AflII C'TTAA_G
Cuts at: 0 2140 2517
Size: 2140 377
AgeI A'CCGG_T
Cuts at: 0 274 2216 2517
Size: 274 1942 301
Fragments arranged by size:
1942 301 274
AleI CACnn'nnGTG
Cuts at: 0 1579 1780 2266 2517
Size: 1579 201 486 251
Fragments arranged by size:
1579 486 251 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 877 2194 2517
Size: 877 1317 323
Fragments arranged by size:
1317 877 323
AlwNI CAG_nnn'CTG
Cuts at: 0 387 1560 2360 2517
Size: 387 1173 800 157
Fragments arranged by size:
1173 800 387 157
ApaI G_GGCC'C
Cuts at: 0 1130 2517
Size: 1130 1387
ApaLI G'TGCA_C
Cuts at: 0 771 1132 2517
Size: 771 361 1385
Fragments arranged by size:
1385 771 361
AvaI C'yCGr_G
Cuts at: 0 1081 2289 2405 2428 2517
Size: 1081 1208 116 23 89
Fragments arranged by size:
1208 1081 116 89 23
BanI G'GyrC_C
Cuts at: 0 277 1091 2517
Size: 277 814 1426
Fragments arranged by size:
1426 814 277
BanII G_rGCy'C
Cuts at: 0 986 1082 1130 2517
Size: 986 96 48 1387
Fragments arranged by size:
1387 986 96 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 328 472 1516 1749 1805 1968 2291 2478
Size: 328 144 1044 233 56 163 323 187
Cuts at: 2478 2517
Size: 39
Fragments arranged by size:
1044 328 323 233 187 163 144 56
39
BbvCI CC'TCA_GC
Cuts at: 0 2480 2517
Size: 2480 37
BclI T'GATC_A
Cuts at: 0 1337 2517
Size: 1337 1180
BlpI GC'TnA_GC
Cuts at: 0 2423 2517
Size: 2423 94
Bme1580I G_kGCm'C
Cuts at: 0 775 1130 1136 2517
Size: 775 355 6 1381
Fragments arranged by size:
1381 775 355 6
BmgBI CAC'GTC
Cuts at: 0 1835 2517
Size: 1835 682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 228 1197 2517
Size: 228 969 1320
Fragments arranged by size:
1320 969 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 86 409 553 997 1045 1210 1448
Size: 75 11 323 144 444 48 165 238
Cuts at: 1448 1927 1963 2517
Size: 479 36 554
Fragments arranged by size:
554 479 444 323 238 165 144 75
48 36 11
Bpu10I CC'TnA_GC
Cuts at: 0 2166 2320 2480 2517
Size: 2166 154 160 37
Fragments arranged by size:
2166 160 154 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 2517
Size: 1585 932
BsaI GGTCTCn'nnnn_
Cuts at: 0 74 1210 1718 2465 2517
Size: 74 1136 508 747 52
Fragments arranged by size:
1136 747 508 74 52
BsaBI GATnn'nnATC
Cuts at: 0 940 1687 2517
Size: 940 747 830
Fragments arranged by size:
940 830 747
BsaHI Gr'CG_yC
Cuts at: 0 680 1006 2306 2517
Size: 680 326 1300 211
Fragments arranged by size:
1300 680 326 211
BsaWI w'CCGG_w
Cuts at: 0 274 2216 2517
Size: 274 1942 301
Fragments arranged by size:
1942 301 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 665 695 1069 1099 2517
Size: 73 30 562 30 374 30 1418
Fragments arranged by size:
1418 562 374 73 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 994 1128 1466 2517
Size: 181 813 134 338 1051
Fragments arranged by size:
1051 813 338 181 134
BseYI C'CCAG_C
Cuts at: 0 113 783 1024 2517
Size: 113 670 241 1493
Fragments arranged by size:
1493 670 241 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 850 943 1107 2517
Size: 601 249 93 164 1410
Fragments arranged by size:
1410 601 249 164 93
BsiEI CG_ry'CG
Cuts at: 0 827 899 1501 2517
Size: 827 72 602 1016
Fragments arranged by size:
1016 827 602 72
BsiHKAI G_wGCw'C
Cuts at: 0 775 986 1136 2406 2517
Size: 775 211 150 1270 111
Fragments arranged by size:
1270 775 211 150 111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 257 627 2517
Size: 257 370 1890
Fragments arranged by size:
1890 370 257
Bsp1286I G_dGCh'C
Cuts at: 0 775 986 1082 1130 1136 2406 2517
Size: 775 211 96 48 6 1270 111
Fragments arranged by size:
1270 775 211 111 96 48 6
BspHI T'CATG_A
Cuts at: 0 1700 2517
Size: 1700 817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 503 1177 1909 2172 2517
Size: 67 436 674 732 263 345
Fragments arranged by size:
732 674 436 345 263 67
BsrBI CCG'CTC
Cuts at: 0 2165 2517
Size: 2165 352
BsrFI r'CCGG_y
Cuts at: 0 274 2216 2517
Size: 274 1942 301
Fragments arranged by size:
1942 301 274
BsrGI T'GTAC_A
Cuts at: 0 926 1331 2517
Size: 926 405 1186
Fragments arranged by size:
1186 926 405
BssSI C'ACGA_G
Cuts at: 0 193 2517
Size: 193 2324
BstEII G'GTnAC_C
Cuts at: 0 651 2517
Size: 651 1866
BstXI CCAn_nnnn'nTGG
Cuts at: 0 783 1782 1905 1932 2517
Size: 783 999 123 27 585
Fragments arranged by size:
999 783 585 123 27
BstYI r'GATC_y
Cuts at: 0 384 1070 1433 2517
Size: 384 686 363 1084
Fragments arranged by size:
1084 686 384 363
Bsu36I CC'TnA_GG
Cuts at: 0 328 2517
Size: 328 2189
BtgI C'CryG_G
Cuts at: 0 122 696 1349 1370 1577 1619 1668 2517
Size: 122 574 653 21 207 42 49 849
Fragments arranged by size:
849 653 574 207 122 49 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 606 2022 2517
Size: 606 1416 495
Fragments arranged by size:
1416 606 495
BtsI GCAGTG_nn'
Cuts at: 0 613 2517
Size: 613 1904
DraIII CAC_nnn'GTG
Cuts at: 0 1115 2517
Size: 1115 1402
DrdI GACnn_nn'nnGTC
Cuts at: 0 1288 2482 2517
Size: 1288 1194 35
Fragments arranged by size:
1288 1194 35
EaeI y'GGCC_r
Cuts at: 0 125 764 824 884 896 1498 1571 2517
Size: 125 639 60 60 12 602 73 946
Fragments arranged by size:
946 639 602 125 73 60 60 12
EagI C'GGCC_G
Cuts at: 0 824 896 1498 2517
Size: 824 72 602 1019
Fragments arranged by size:
1019 824 602 72
EarI CTCTTCn'nnn_
Cuts at: 0 409 2125 2517
Size: 409 1716 392
Fragments arranged by size:
1716 409 392
EcoICRI GAG'CTC
Cuts at: 0 984 2517
Size: 984 1533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 86 409 553 580 991 997 1045
Size: 75 11 323 144 27 411 6 48
Cuts at: 1045 1210 1448 1575 1927 1963 2211 2485 2517
Size: 165 238 127 352 36 248 274 32
Fragments arranged by size:
411 352 323 274 248 238 165 144
127 75 48 36 32 27 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 458 877 1362 2517
Size: 458 419 485 1155
Fragments arranged by size:
1155 485 458 419
EcoO109I rG'GnC_Cy
Cuts at: 0 1852 1865 2517
Size: 1852 13 652
Fragments arranged by size:
1852 652 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1317 1992 2517
Size: 1317 675 525
Fragments arranged by size:
1317 675 525
HaeII r_GCGC'y
Cuts at: 0 839 2517
Size: 839 1678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 665 697 1209 1241 1423 1455
Size: 73 32 560 32 512 32 182 32
Cuts at: 1455 1819 1851 2401 2433 2517
Size: 364 32 550 32 84
Fragments arranged by size:
560 550 512 364 182 84 73 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 573 2061 2517
Size: 573 1488 456
Fragments arranged by size:
1488 573 456
MscI TGG'CCA
Cuts at: 0 1573 2517
Size: 1573 944
MslI CAynn'nnrTG
Cuts at: 0 1579 1780 1930 2266 2517
Size: 1579 201 150 336 251
Fragments arranged by size:
1579 336 251 201 150
MspA1I CmG'CkG
Cuts at: 0 34 783 1011 1024 1065 1254 1670 1780
Size: 34 749 228 13 41 189 416 110
Cuts at: 1780 2517
Size: 737
Fragments arranged by size:
749 737 416 228 189 110 41 34
13
NcoI C'CATG_G
Cuts at: 0 696 1349 1619 2517
Size: 696 653 270 898
Fragments arranged by size:
898 696 653 270
NspI r_CATG'y
Cuts at: 0 927 1239 1656 2034 2517
Size: 927 312 417 378 483
Fragments arranged by size:
927 483 417 378 312
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 1683 2517
Size: 387 1296 834
Fragments arranged by size:
1296 834 387
PfoI T'CCnGG_A
Cuts at: 0 530 1073 1099 2117 2517
Size: 530 543 26 1018 400
Fragments arranged by size:
1018 543 530 400 26
PspOMI G'GGCC_C
Cuts at: 0 1126 2517
Size: 1126 1391
PstI C_TGCA'G
Cuts at: 0 413 809 1271 1493 1556 1864 2102 2183
Size: 413 396 462 222 63 308 238 81
Cuts at: 2183 2517
Size: 334
Fragments arranged by size:
462 413 396 334 308 238 222 81
63
PvuII CAG'CTG
Cuts at: 0 34 783 1024 2517
Size: 34 749 241 1493
Fragments arranged by size:
1493 749 241 34
SacI G_AGCT'C
Cuts at: 0 986 2517
Size: 986 1531
SacII CC_GC'GG
Cuts at: 0 1671 2517
Size: 1671 846
SapI GCTCTTCn'nnn_
Cuts at: 0 409 2125 2517
Size: 409 1716 392
Fragments arranged by size:
1716 409 392
SbfI CC_TGCA'GG
Cuts at: 0 1271 1864 2517
Size: 1271 593 653
Fragments arranged by size:
1271 653 593
SfcI C'TryA_G
Cuts at: 0 409 805 1203 1267 1362 1489 1530 1552
Size: 409 396 398 64 95 127 41 22
Cuts at: 1552 1860 2098 2179 2517
Size: 308 238 81 338
Fragments arranged by size:
409 398 396 338 308 238 127 95
81 64 41 22
SgrAI Cr'CCGG_yG
Cuts at: 0 274 2517
Size: 274 2243
SmaI CCC'GGG
Cuts at: 0 2291 2517
Size: 2291 226
SmlI C'TyrA_G
Cuts at: 0 1600 2140 2405 2517
Size: 1600 540 265 112
Fragments arranged by size:
1600 540 265 112
StuI AGG'CCT
Cuts at: 0 2158 2517
Size: 2158 359
StyI C'CwwG_G
Cuts at: 0 696 1349 1619 1637 2517
Size: 696 653 270 18 880
Fragments arranged by size:
880 696 653 270 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2038 2517
Size: 2038 479
TatI w'GTAC_w
Cuts at: 0 926 1331 1586 1892 2517
Size: 926 405 255 306 625
Fragments arranged by size:
926 625 405 306 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1069 1101 2517
Size: 1069 32 1416
Fragments arranged by size:
1416 1069 32
Tth111I GACn'n_nGTC
Cuts at: 0 301 2517
Size: 301 2216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 2115 2517
Size: 846 1269 402
Fragments arranged by size:
1269 846 402
XhoI C'TCGA_G
Cuts at: 0 2405 2517
Size: 2405 112
XmaI C'CCGG_G
Cuts at: 0 2289 2517
Size: 2289 228
XmnI GAAnn'nnTTC
Cuts at: 0 2134 2158 2517
Size: 2134 24 359
Fragments arranged by size:
2134 359 24
ZraI GAC'GTC
Cuts at: 0 681 2517
Size: 681 1836
Enzymes that do cut:
AarI AatII AccI AcuI AflII AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI
BclI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII MscI MslI
MspA1I NcoI NspI PflMI PfoI PspOMI PstI PvuII
SacI SacII SapI SbfI SfcI SgrAI SmaI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BfrBI BglI BglII BmrI BmtI BsaAI BsiWI BsmI
BspEI BsrDI BssHII BstAPI BstBI BstZ17I ClaI CspCI
DraI EciI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI
XbaI