################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccII AcyI AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI Asp718I AviII AvrII BbeI BcgI BlnI BsaHI BsaI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI Bsu36I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI FspI HaeII Hin1I KasI KpnI KspI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PvuI RsrII SacII SbfI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AclI 3305 AACGTT AA^CGTT AflII 5887 CTTAAG C^TTAAG AhdI 2552 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 65 GGGCCC GGGCC^C AseI 5106 ATTAAT AT^TAAT AsnI 5106 ATTAAT AT^TAAT AspEI 2552 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 5260 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 5111 TGGCCA TGG^CCA BbrPI 2378 CACGTG CAC^GTG BciVI 3237 GGATAC GTATCC(6/5) BclI 5655 TGATCA T^GATCA BfrI 5887 CTTAAG C^TTAAG BglI 3027 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 943 GCTGAGC GC^T[ACGT]AGC Bpu1102I 943 GCTGAGC GC^T[ACGT]AGC BsaAI 2378 CACGTG [CT]AC^GT[GA] BsmI 5641 GAATGC GAATGC(1/-1) BsrFI 8 ACCGGC [GA]^CCGG[CT] Bst1107I 4100 GTATAC GTA^TAC BstZ17I 4100 GTATAC GTA^TAC CelII 943 GCTGAGC GC^T[ACGT]AGC Cfr10I 8 ACCGGC [GA]^CCGG[CT] DraI 3771 TTTAAA TTT^AAA DraIII 650 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 5111 TGGCCA [CT]^GGCC[GA] Eam1105I 2552 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1711 GAATTC G^AATTC FbaI 5655 TGATCA T^GATCA HgaI 709 GCGTC GACGC(5/10) HpaI 4521 GTTAAC GTT^AAC MluNI 5111 TGGCCA TGG^CCA MscI 5111 TGGCCA TGG^CCA MslI 4068 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 2858 CCATGG C^CATGG PflFI 5260 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3589 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2378 CACGTG CAC^GTG PmlI 2378 CACGTG CAC^GTG PshBI 5106 ATTAAT AT^TAAT Psp1406I 3305 AACGTT AA^CGTT SacI 946 GAGCTC GAGCT^C SalI 1052 GTCGAC G^TCGAC SexAI 651 ACCAGGT A^CC[AT]GGT SfiI 3026 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Tth111I 5260 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3589 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccIII 2481 TCCGGA T^CCGGA 5786 TCCGGA AvaI 206 CTCGAG C^[CT]CG[GA]G 2456 CTCGAG BsaBI 2849 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5531 GATTTGAATC BsaWI 2481 TCCGGA [AT]^CCGG[AT] 5786 TCCGGA BseAI 2481 TCCGGA T^CCGGA 5786 TCCGGA BsgI 371 CTGCAC GTGCAG(16/14) 500 GTGCAG BsoBI 206 CTCGAG C^[CT]CG[GA]G 2456 CTCGAG BspEI 2481 TCCGGA T^CCGGA 5786 TCCGGA BspHI 4565 TCATGA T^CATGA 5670 TCATGA DsaI 983 CCACGG C^C[GA][CT]GG 2858 CCATGG EcoNI 5039 CCTTTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5773 CCTGTTGGAGG FauI 1678 GCGGG CCCGC(4/6) 3202 GCGGG HindIII 1208 AAGCTT A^AGCTT 3129 AAGCTT MamI 2849 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5531 GATTTGAATC MroI 2481 TCCGGA T^CCGGA 5786 TCCGGA PaeR7I 206 CTCGAG C^TCGAG 2456 CTCGAG PpuMI 2550 AGGACCT [GA]G^G[AT]CC[CT] 4820 AGGACCT PshAI 4936 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5808 GACAATTGTC RcaI 4565 TCATGA T^CATGA 5670 TCATGA ScaI 3041 AGTACT AGT^ACT 5564 AGTACT SpeI 2422 ACTAGT A^CTAGT 4498 ACTAGT StuI 1622 AGGCCT AGG^CCT 5650 AGGCCT XhoI 206 CTCGAG C^TCGAG 2456 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI