Restriction map for
pFN21AA0045
(Circular) (Six-base) MAPSORT of: pFN21AA0045.seq Check: 2652 from: 1 to: 10681
pFN21AA0045 10681 bp
With 182 enzymes: SgfI *
March 28, 2012 12:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2179 2953 2179
Size: 774 9907
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8491 278
Size: 53 83 186 936 6955 2468
Fragments arranged by size:
6955 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3058 5076 6106 7321 8024 1937
Size: 1121 2018 1030 1215 703 4594
Fragments arranged by size:
4594 2018 1215 1121 1030 703
Acc65I G'GTAC_C
Cuts at: 8002 8002
Size: 10681
AclI AA'CG_TT
Cuts at: 1563 5311 8808 9181 1563
Size: 3748 3497 373 3063
Fragments arranged by size:
3748 3497 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 2273 3069 3093 3353 3455 3706 4646
Size: 519 122 796 24 260 102 251 940
Cuts at: 4646 4697 4712 4750 4863 5348 6135 7436 7550
Size: 51 15 38 113 485 787 1301 114
Cuts at: 7550 8744 10128 10396 1632
Size: 1194 1384 268 1917
Fragments arranged by size:
1917 1384 1301 1194 940 796 787 519
485 268 260 251 122 114 113 102
51 38 24 15
AflII C'TTAA_G
Cuts at: 820 1017 7892 820
Size: 197 6875 3609
Fragments arranged by size:
6875 3609 197
AflIII A'CryG_T
Cuts at: 1203 9580 10557 1203
Size: 8377 977 1327
Fragments arranged by size:
8377 1327 977
AgeI A'CCGG_T
Cuts at: 9512 9512
Size: 10681
AhdI GACnn_n'nnGTC
Cuts at: 4562 9410 4562
Size: 4848 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3781 1184
Size: 2597 8084
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2449 2481 2449
Size: 32 10649
AlwNI CAG_nnn'CTG
Cuts at: 3080 3152 3397 4559 7030 8424 9996 3080
Size: 72 245 1162 2471 1394 1572 3765
Fragments arranged by size:
3765 2471 1572 1394 1162 245 72
ApaI G_GGCC'C
Cuts at: 2074 2074
Size: 10681
ApaLI G'TGCA_C
Cuts at: 8738 9894 8738
Size: 1156 9525
ApoI r'AATT_y
Cuts at: 1493 2780 3103 3716 3791 3916 4725 5300 5780
Size: 1287 323 613 75 125 809 575 480
Cuts at: 5780 6010 6509 7990 8216 8368 10477 1493
Size: 230 499 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 1287 809 613 575 499
480 323 230 226 152 125 75
AseI AT'TA_AT
Cuts at: 160 1032 7112 9235 160
Size: 872 6080 2123 1606
Fragments arranged by size:
6080 2123 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10681
AvaI C'yCGr_G
Cuts at: 1870 1943 2211 4461 8006 1870
Size: 73 268 2250 3545 4545
Fragments arranged by size:
4545 3545 2250 268 73
BamHI G'GATC_C
Cuts at: 2146 4483 4859 4868 8011 8378 2146
Size: 2337 376 9 3143 367 4449
Fragments arranged by size:
4449 3143 2337 376 367 9
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 2660 4115 8002 9457
Size: 325 217 627 404 469 1455 3887 1455
Cuts at: 9457 618
Size: 1842
Fragments arranged by size:
3887 1842 1455 1455 627 469 404 325
217
BanII G_rGCy'C
Cuts at: 1391 2074 2955 2996 5613 6262 8000 1391
Size: 683 881 41 2617 649 1738 4072
Fragments arranged by size:
4072 2617 1738 881 683 649 41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2613 2841 3227 5955 6405 7269 7540
Size: 971 714 228 386 2728 450 864 271
Cuts at: 7540 928
Size: 4069
Fragments arranged by size:
4069 2728 971 864 714 450 386 271
228
BbvCI CC'TCA_GC
Cuts at: 2557 4549 5681 2557
Size: 1992 1132 7557
Fragments arranged by size:
7557 1992 1132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8872 8906 1903
Size: 34 6935 34 3678
Fragments arranged by size:
6935 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5236 8573 9783 5236
Size: 3337 1210 6134
Fragments arranged by size:
6134 3337 1210
BclI T'GATC_A
Cuts at: 1548 7660 1548
Size: 6112 4569
BfrBI ATG'CAT
Cuts at: 3746 4786 7331 3746
Size: 1040 2545 7096
Fragments arranged by size:
7096 2545 1040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5038 9292 136
Size: 107 122 71 631 605 3366 4254 1525
Fragments arranged by size:
4254 3366 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2516 3943 4969 7369 10676 2516
Size: 1427 1026 2400 3307 2521
Fragments arranged by size:
3307 2521 2400 1427 1026
BlpI GC'TnA_GC
Cuts at: 2949 8098 2949
Size: 5149 5532
Bme1580I G_kGCm'C
Cuts at: 1790 2074 6335 7492 8742 9898 1790
Size: 284 4261 1157 1250 1156 2573
Fragments arranged by size:
4261 2573 1250 1157 1156 284
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2952 3431 7226
Size: 416 53 83 399 24 1535 479 3795
Cuts at: 7226 8412 9370 442
Size: 1186 958 1753
Fragments arranged by size:
3795 1753 1535 1186 958 479 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10681
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6168 6168
Size: 10681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3908 5384 5622 6920 7007 7733 9341 1239
Size: 2669 1476 238 1298 87 726 1608 2579
Fragments arranged by size:
2669 2579 1608 1476 1298 726 238 87
Bpu10I CC'TnA_GC
Cuts at: 2557 4549 5681 2557
Size: 1992 1132 7557
Fragments arranged by size:
7557 1992 1132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4152 4965 6720 8806 9671 9969 4152
Size: 813 1755 2086 865 298 4864
Fragments arranged by size:
4864 2086 1755 865 813 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 9344 882
Size: 617 7845 2219
Fragments arranged by size:
7845 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4385 8472 493
Size: 711 3181 4087 2702
Fragments arranged by size:
4087 3181 2702 711
BsaBI GATnn'nnATC
Cuts at: 4858 7540 10675 4858
Size: 2682 3135 4864
Fragments arranged by size:
4864 3135 2682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8488 8870 9505 275
Size: 53 83 186 936 6955 382 635 1451
Fragments arranged by size:
6955 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4486 7791 9114 9512 9786 9933 10344 4486
Size: 3305 1323 398 274 147 411 4823
Fragments arranged by size:
4823 3305 1323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2242 2272 2287 2317 3635
Size: 30 165 30 839 30 15 30 1318
Cuts at: 3635 3649 3665 3679 5382 5412 1178
Size: 14 16 14 1703 30 6447
Fragments arranged by size:
6447 1703 1318 839 165 30 30 30
30 30 16 15 14 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2229 2605 2632 3054 3428 3926 6806 7475
Size: 1043 376 27 422 374 498 2880 669
Cuts at: 7475 1186
Size: 4392
Fragments arranged by size:
4392 2880 1043 669 498 422 376 374
27
BseYI C'CCAG_C
Cuts at: 2567 2723 3321 4937 9884 10380 2567
Size: 156 598 1616 4947 496 2868
Fragments arranged by size:
4947 2868 1616 598 496 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2361 2526 1163
Size: 573 625 165 9318
Fragments arranged by size:
9318 625 573 165
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8131 8892 9920 1540
Size: 275 186 6130 761 1028 2301
Fragments arranged by size:
6130 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2955 2975 3483 4468 4711 8742 8827 9898 2955
Size: 20 508 985 243 4031 85 1071 3738
Fragments arranged by size:
4031 3738 1071 985 508 243 85 20
BsmI GAATG_Cn'
Cuts at: 7652 8192 8285 7652
Size: 540 93 10048
Fragments arranged by size:
10048 540 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2955 2975 2996 3483 4468 4711
Size: 399 284 881 20 21 487 985 243
Cuts at: 4711 5613 6262 6335 7492 8000 8742 8827 9898
Size: 902 649 73 1157 508 742 85 1071
Cuts at: 9898 1391
Size: 2174
Fragments arranged by size:
2174 1157 1071 985 902 881 742 649
508 487 399 284 243 85 73 21
20
BspEI T'CCGG_A
Cuts at: 4486 7791 10344 4486
Size: 3305 2553 4823
Fragments arranged by size:
4823 3305 2553
BspHI T'CATG_A
Cuts at: 6570 7675 8570 10300 6570
Size: 1105 895 1730 6951
Fragments arranged by size:
6951 1730 1105 895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2179 2953 3919 4567 6623 8036 844
Size: 1335 774 966 648 2056 1413 3489
Fragments arranged by size:
3489 2056 1413 1335 966 774 648
BsrBI CCG'CTC
Cuts at: 1394 1823 8568 1394
Size: 429 6745 3507
Fragments arranged by size:
6745 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3818 4083 4662 6486 6693 9176 9350
Size: 1183 2570 265 579 1824 207 2483 174
Cuts at: 9350 65
Size: 1396
Fragments arranged by size:
2570 2483 1824 1396 1183 579 265 207
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 9325 9512 1700
Size: 110 203 7312 187 2869
Fragments arranged by size:
7312 2869 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 10681
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10681
BssSI C'ACGA_G
Cuts at: 8741 9753 8741
Size: 1012 9669
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4559 5204 5506 794
Size: 3765 645 302 5969
Fragments arranged by size:
5969 3765 645 302
BstBI TT'CG_AA
Cuts at: 9485 9485
Size: 10681
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10681
BstXI CCAn_nnnn'nTGG
Cuts at: 5151 5408 5486 5151
Size: 257 78 10346
Fragments arranged by size:
10346 257 78
BstYI r'GATC_y
Cuts at: 1972 2146 2516 3943 4483 4859 4868 4969 6556
Size: 174 370 1427 540 376 9 101 1587
Cuts at: 6556 7369 8011 8378 8763 8780 10232 10318 10330
Size: 813 642 367 385 17 1452 86 12
Cuts at: 10330 10676 1972
Size: 346 1977
Fragments arranged by size:
1977 1587 1452 1427 813 642 540 385
376 370 367 346 174 101 86 17
12 9
BstZ17I GTA'TAC
Cuts at: 6107 6107
Size: 10681
BtgI C'CryG_G
Cuts at: 513 1065 2988 4863 513
Size: 552 1923 1875 6331
Fragments arranged by size:
6331 1923 1875 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5970 487
Size: 683 56 4744 5198
Fragments arranged by size:
5198 4744 683 56
BtsI GCAGTG_nn'
Cuts at: 2039 2061 2233 2938 4301 4894 5852 8197 8991
Size: 22 172 705 1363 593 958 2345 794
Cuts at: 8991 9011 2039
Size: 20 3709
Fragments arranged by size:
3709 2345 1363 958 794 705 593 172
22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10681
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10646
DraI TTT'AAA
Cuts at: 5778 7985 5778
Size: 2207 8474
DraIII CAC_nnn'GTG
Cuts at: 2660 2660
Size: 10681
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9688 809
Size: 507 8372 1802
Fragments arranged by size:
8372 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 7116 8128 9017
Size: 54 1425 50 275 18 5286 1012 889
Cuts at: 9017 10646 8
Size: 1629 43
Fragments arranged by size:
5286 1629 1425 1012 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8128 1537
Size: 275 6316 4090
Fragments arranged by size:
6316 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 2408 2505 2779 2844 4765 5601 5614
Size: 983 72 97 274 65 1921 836 13
Cuts at: 5614 5923 6250 8611 1353
Size: 309 327 2361 3423
Fragments arranged by size:
3423 2361 1921 983 836 327 309 274
97 72 65 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2983 6824 9268 9642 9788 387
Size: 1064 138 1394 3841 2444 374 146 1280
Fragments arranged by size:
3841 2444 1394 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2953 2953
Size: 10681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2273 3069 3093 3353 3455 3706
Size: 393 519 122 796 24 260 102 251
Cuts at: 3706 3908 4646 4697 4712 4750 4863 5348 5384
Size: 202 738 51 15 38 113 485 36
Cuts at: 5384 5622 6135 6920 7007 7436 7550 7733 8744
Size: 238 513 785 87 429 114 183 1011
Cuts at: 8744 9341 10128 10396 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1011 796 787 785 738 597 519
513 485 429 393 268 260 251 238
202 183 122 114 113 102 87 51
38 36 24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 7048 7782 7048
Size: 734 9947
EcoO109I rG'GnC_Cy
Cuts at: 2070 4312 4556 6826 7027 2070
Size: 2242 244 2270 201 5724
Fragments arranged by size:
5724 2270 2242 244 201
EcoRI G'AATT_C
Cuts at: 3716 7990 8368 3716
Size: 4274 378 6029
Fragments arranged by size:
6029 4274 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2834 3965 6989 7114 2834
Size: 1131 3024 125 6401
Fragments arranged by size:
6401 3024 1131 125
FspI TGC'GCA
Cuts at: 9187 9187
Size: 10681
HaeII r_GCGC'y
Cuts at: 1678 9828 1678
Size: 8150 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2242 2274 3167 3199 3635
Size: 32 135 32 670 32 893 32 436
Cuts at: 3635 3667 3839 3871 4548 4580 4916 4948 5234
Size: 32 172 32 677 32 336 32 286
Cuts at: 5234 5266 5450 5482 5860 5892 5951 5983 6067
Size: 32 184 32 378 32 59 32 84
Cuts at: 6067 6099 6166 6198 7786 7818 9322 9354 9396
Size: 32 67 32 1588 32 1504 32 42
Cuts at: 9396 9428 1373
Size: 32 2626
Fragments arranged by size:
2626 1588 1504 893 677 670 436 378
336 286 184 172 135 84 67 59
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3059 3362 4824 6528 8025 8271
Size: 988 281 1121 303 1462 1704 1497 246
Cuts at: 8271 669
Size: 3079
Fragments arranged by size:
3079 1704 1497 1462 1121 988 303 281
246
HindIII A'AGCT_T
Cuts at: 748 3213 5134 748
Size: 2465 1921 6295
Fragments arranged by size:
6295 2465 1921
HpaI GTT'AAC
Cuts at: 6528 8271 6528
Size: 1743 8938
KpnI G_GTAC'C
Cuts at: 8006 8006
Size: 10681
MfeI C'AATT_G
Cuts at: 3294 3753 7815 8280 3294
Size: 459 4062 465 5695
Fragments arranged by size:
5695 4062 465 459
MluI A'CGCG_T
Cuts at: 10557 10557
Size: 10681
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 4068 4076 5199 5258 5503 6296 6524
Size: 729 2034 8 1123 59 245 793 228
Cuts at: 6524 7762 9795 9979 1305
Size: 1238 2033 184 2007
Fragments arranged by size:
2034 2033 2007 1238 1123 793 729 245
228 184 59 8
MscI TGG'CCA
Cuts at: 10 64 1489 1832 7118 10
Size: 54 1425 343 5286 3573
Fragments arranged by size:
5286 3573 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6077 8639 8998 9157 518
Size: 648 4911 2562 359 159 2042
Fragments arranged by size:
4911 2562 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3047 3113 3967 4904 4922 5503 8097 8774
Size: 1441 66 854 937 18 581 2594 677
Cuts at: 8774 9922 10167 1606
Size: 1148 245 2120
Fragments arranged by size:
2594 2120 1441 1148 937 854 677 581
245 66 18
NcoI C'CATG_G
Cuts at: 513 1065 4863 513
Size: 552 3798 6331
Fragments arranged by size:
6331 3798 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10681
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10681
NotI GC'GGCC_GC
Cuts at: 8128 8128
Size: 10681
NsiI A_TGCA'T
Cuts at: 3748 4788 7333 3748
Size: 1040 2545 7096
Fragments arranged by size:
7096 2545 1040
NspI r_CATG'y
Cuts at: 1132 1229 4027 4845 6443 8039 9584 1132
Size: 97 2798 818 1598 1596 1545 2229
Fragments arranged by size:
2798 2229 1598 1596 1545 818 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10681
PciI A'CATG_T
Cuts at: 9580 9580
Size: 10681
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5600 1108
Size: 4492 6189
PfoI T'CCnGG_A
Cuts at: 5599 5599
Size: 10681
PmeI GTTT'AAAC
Cuts at: 7985 7985
Size: 10681
PmlI CAC'GTG
Cuts at: 4385 4385
Size: 10681
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8750 8782 10263 10295 8750
Size: 32 1481 32 9136
Fragments arranged by size:
9136 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4556 6826 4556
Size: 2270 8411
PshAI GACnn'nnGTC
Cuts at: 1613 6945 7817 1613
Size: 5332 872 4477
Fragments arranged by size:
5332 4477 872
PsiI TTA'TAA
Cuts at: 4010 7173 8251 4010
Size: 3163 1078 6440
Fragments arranged by size:
6440 3163 1078
PspOMI G'GGCC_C
Cuts at: 2070 2070
Size: 10681
PspXI vC'TCGA_Gb
Cuts at: 4461 4461
Size: 10681
PstI C_TGCA'G
Cuts at: 830 2111 3148 4921 6039 8033 830
Size: 1281 1037 1773 1118 1994 3478
Fragments arranged by size:
3478 1994 1773 1281 1118 1037
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10681
PvuII CAG'CTG
Cuts at: 3047 3113 3967 4904 4922 5503 3047
Size: 66 854 937 18 581 8225
Fragments arranged by size:
8225 937 854 581 66 18
SacI G_AGCT'C
Cuts at: 2955 2955
Size: 10681
SalI G'TCGA_C
Cuts at: 1936 3057 8023 1936
Size: 1121 4966 4594
Fragments arranged by size:
4966 4594 1121
SapI GCTCTTCn'nnn_
Cuts at: 2779 5601 6250 2779
Size: 2822 649 7210
Fragments arranged by size:
7210 2822 649
SbfI CC_TGCA'GG
Cuts at: 8033 8033
Size: 10681
ScaI AGT'ACT
Cuts at: 5048 7571 8929 10641 5048
Size: 2523 1358 1712 5088
Fragments arranged by size:
5088 2523 1712 1358
SexAI A'CCwGG_T
Cuts at: 2656 2656
Size: 10681
SfcI C'TryA_G
Cuts at: 826 1045 2107 2628 3144 3677 4917 6035 6929
Size: 219 1062 521 516 533 1240 1118 894
Cuts at: 6929 7322 7651 8029 9164 9845 10036 826
Size: 393 329 378 1135 681 191 1471
Fragments arranged by size:
1471 1240 1135 1118 1062 894 681 533
521 516 393 378 329 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 5038 5038
Size: 10681
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10681
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10681
SmaI CCC'GGG
Cuts at: 1872 8008 1872
Size: 6136 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2211 4167 4461 4980 6735 7892
Size: 197 926 268 1956 294 519 1755 1157
Cuts at: 7892 8785 9686 9948 820
Size: 893 901 262 1553
Fragments arranged by size:
1956 1755 1553 1157 926 901 893 519
294 268 262 197
SnaBI TAC'GTA
Cuts at: 493 8472 493
Size: 7979 2702
SpeI A'CTAG_T
Cuts at: 152 4427 6503 152
Size: 4275 2076 4330
Fragments arranged by size:
4330 4275 2076
SphI G_CATG'C
Cuts at: 1132 8039 1132
Size: 6907 3774
SspI AAT'ATT
Cuts at: 5 52 8605 5
Size: 47 8553 2081
Fragments arranged by size:
8553 2081 47
StuI AGG'CCT
Cuts at: 1517 3629 7657 1517
Size: 2112 4028 4541
Fragments arranged by size:
4541 4028 2112
StyI C'CwwG_G
Cuts at: 513 1065 3136 3639 4863 7119 7156 8120 513
Size: 552 2071 503 1224 2256 37 964 3074
Fragments arranged by size:
3074 2256 2071 1224 964 552 503 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2559 4000 4951 6696 8708 8725 8878
Size: 1255 979 1441 951 1745 2012 17 153
Cuts at: 8878 9063 9508 325
Size: 185 445 1498
Fragments arranged by size:
2012 1745 1498 1441 1255 979 951 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5046 5165 5710
Size: 274 80 33 51 1443 3069 119 545
Cuts at: 5710 7569 8927 10639 96
Size: 1859 1358 1712 138
Fragments arranged by size:
3069 1859 1712 1443 1358 545 274 138
119 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3267 3299 4652 4684 10641 10673 3267
Size: 32 1353 32 5957 32 3275
Fragments arranged by size:
5957 3275 1353 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7268 1316
Size: 5952 4729
XbaI T'CTAG_A
Cuts at: 8017 8017
Size: 10681
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3293 5408 5620 7126 7757 3293
Size: 2115 212 1506 631 6217
Fragments arranged by size:
6217 2115 1506 631 212
XhoI C'TCGA_G
Cuts at: 1943 2211 4461 1943
Size: 268 2250 8163
Fragments arranged by size:
8163 2250 268
XmaI C'CCGG_G
Cuts at: 1870 8006 1870
Size: 6136 4545
XmnI GAAnn'nnTTC
Cuts at: 3537 4095 5697 6495 8810 3537
Size: 558 1602 798 2315 5408
Fragments arranged by size:
5408 2315 1602 798 558
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8489 276
Size: 53 83 186 936 6955 2468
Fragments arranged by size:
6955 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BsmBI Bsu36I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PsrI RsrII SacII SanDI SfoI SrfI SwaI