Restriction map for pFN21AA0045
 (Linear) (Six-base) MAPSORT of: KIAA0045.seq  Check: 8491  from: 1  to: 5976



KIAA0045.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     174     948    5976 
   Size:        174     774    5028

  Fragments arranged by size: 

                5028     774     174
 
AccI GT'mk_AC

Cuts at:      0    1053    3071    4101    5316    5976 
   Size:       1053    2018    1030    1215     660

  Fragments arranged by size: 

                2018    1215    1053    1030     660
 
AclI AA'CG_TT

Cuts at:      0    3306    5976 
   Size:       3306    2670
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     268    1064    1088    1348    1450    1701    2641 
   Size:        146     122     796      24     260     102     251     940

Cuts at:   2641    2692    2707    2745    2858    3343    4130    5431    5545 
   Size:         51      15      38     113     485     787    1301     114

Cuts at:   5545    5976 
   Size:        431

  Fragments arranged by size: 

                1301     940     796     787     485     431     260     251
                 146     122     114     113     102      51      38      24
                  15
 
AflII C'TTAA_G

Cuts at:      0    5887    5976 
   Size:       5887      89
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2557    5976 
   Size:       2557    3419
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1776    5976 
   Size:       1776    4200
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     444     476    5976 
   Size:        444      32    5500

  Fragments arranged by size: 

                5500     444      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1075    1147    1392    2554    5025    5976 
   Size:       1075      72     245    1162    2471     951

  Fragments arranged by size: 

                2471    1162    1075     951     245      72
 
ApaI G_GGCC'C

Cuts at:      0      69    5976 
   Size:         69    5907
 
ApoI r'AATT_y

Cuts at:      0     775    1098    1711    1786    1911    2720    3295    3775 
   Size:        775     323     613      75     125     809     575     480

Cuts at:   3775    4005    4504    5976 
   Size:        230     499    1472

  Fragments arranged by size: 

                1472     809     775     613     575     499     480     323
                 230     125      75
 
AseI AT'TA_AT

Cuts at:      0    5107    5976 
   Size:       5107     869
 
AvaI C'yCGr_G

Cuts at:      0     206    2456    5976 
   Size:        206    2250    3520

  Fragments arranged by size: 

                3520    2250     206
 
BamHI G'GATC_C

Cuts at:      0     141    2478    2854    2863    5976 
   Size:        141    2337     376       9    3113

  Fragments arranged by size: 

                3113    2337     376     141       9
 
BanI G'GyrC_C

Cuts at:      0     186     655    2110    5976 
   Size:        186     469    1455    3866

  Fragments arranged by size: 

                3866    1455     469     186
 
BanII G_rGCy'C

Cuts at:      0      69     950     991    3608    4257    5976 
   Size:         69     881      41    2617     649    1719

  Fragments arranged by size: 

                2617    1719     881     649      69      41
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     608     836    1222    3950    4400    5264    5535    5976 
   Size:        608     228     386    2728     450     864     271     441

  Fragments arranged by size: 

                2728     864     608     450     441     386     271     228
            
 
BbvCI CC'TCA_GC

Cuts at:      0     552    2544    3676    5976 
   Size:        552    1992    1132    2300

  Fragments arranged by size: 

                2300    1992    1132     552
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3231    5976 
   Size:       3231    2745
 
BclI T'GATC_A

Cuts at:      0    5655    5976 
   Size:       5655     321
 
BfrBI ATG'CAT

Cuts at:      0    1741    2781    5326    5976 
   Size:       1741    1040    2545     650

  Fragments arranged by size: 

                2545    1741    1040     650
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3033    5976 
   Size:       3033    2943
 
BglII A'GATC_T

Cuts at:      0     511    1938    2964    5364    5976 
   Size:        511    1427    1026    2400     612

  Fragments arranged by size: 

                2400    1427    1026     612     511
 
BlpI GC'TnA_GC

Cuts at:      0     944    5976 
   Size:        944    5032
 
Bme1580I G_kGCm'C

Cuts at:      0      69    4330    5487    5976 
   Size:         69    4261    1157     489

  Fragments arranged by size: 

                4261    1157     489      69
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     947    1426    5221    5976 
   Size:        947     479    3795     755

  Fragments arranged by size: 

                3795     947     755     479
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4163    5976 
   Size:       4163    1813
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1903    3379    3617    4915    5002    5728    5976 
   Size:       1903    1476     238    1298      87     726     248

  Fragments arranged by size: 

                1903    1476    1298     726     248     238      87
 
Bpu10I CC'TnA_GC

Cuts at:      0     552    2544    3676    5976 
   Size:        552    1992    1132    2300

  Fragments arranged by size: 

                2300    1992    1132     552
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2147    2960    4715    5976 
   Size:       2147     813    1755    1261

  Fragments arranged by size: 

                2147    1755    1261     813
 
BsaAI yAC'GTr

Cuts at:      0    2380    5976 
   Size:       2380    3596
 
BsaBI GATnn'nnATC

Cuts at:      0    2853    5535    5976 
   Size:       2853    2682     441

  Fragments arranged by size: 

                2853    2682     441
 
BsaWI w'CCGG_w

Cuts at:      0    2481    5786    5976 
   Size:       2481    3305     190

  Fragments arranged by size: 

                3305    2481     190
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     237     267     282     312    1630    1644    1660    1674 
   Size:        237      30      15      30    1318      14      16      14

Cuts at:   1674    3377    3407    5976 
   Size:       1703      30    2569

  Fragments arranged by size: 

                2569    1703    1318     237      30      30      30      16
                  15      14      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     224     600     627    1049    1423    1921    4801    5470 
   Size:        224     376      27     422     374     498    2880     669

Cuts at:   5470    5976 
   Size:        506

  Fragments arranged by size: 

                2880     669     506     498     422     376     374     224
                  27
 
BseYI C'CCAG_C

Cuts at:      0     562     718    1316    2932    5976 
   Size:        562     156     598    1616    3044

  Fragments arranged by size: 

                3044    1616     598     562     156
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     521    5976 
   Size:        356     165    5455

  Fragments arranged by size: 

                5455     356     165
 
BsiHKAI G_wGCw'C

Cuts at:      0     950     970    1478    2463    2706    5976 
   Size:        950      20     508     985     243    3270

  Fragments arranged by size: 

                3270     985     950     508     243      20
 
BsmI GAATG_Cn'

Cuts at:      0    5647    5976 
   Size:       5647     329
 
Bsp1286I G_dGCh'C

Cuts at:      0      69     950     970     991    1478    2463    2706    3608 
   Size:         69     881      20      21     487     985     243     902

Cuts at:   3608    4257    4330    5487    5976 
   Size:        649      73    1157     489

  Fragments arranged by size: 

                1157     985     902     881     649     489     487     243
                  73      69      21      20
 
BspEI T'CCGG_A

Cuts at:      0    2481    5786    5976 
   Size:       2481    3305     190

  Fragments arranged by size: 

                3305    2481     190
 
BspHI T'CATG_A

Cuts at:      0    4565    5670    5976 
   Size:       4565    1105     306

  Fragments arranged by size: 

                4565    1105     306
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     174     948    1914    2562    4618    5976 
   Size:        174     774     966     648    2056    1358

  Fragments arranged by size: 

                2056    1358     966     774     648     174
 
BsrDI GCAATG_nn'

Cuts at:      0    1813    2078    2657    4481    4688    5976 
   Size:       1813     265     579    1824     207    1288

  Fragments arranged by size: 

                1824    1813    1288     579     265     207
 
BsrFI r'CCGG_y

Cuts at:      0       8    5976 
   Size:          8    5968
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2554    3199    3501    5976 
   Size:       2554     645     302    2475

  Fragments arranged by size: 

                2554    2475     645     302
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3146    3403    3481    5976 
   Size:       3146     257      78    2495

  Fragments arranged by size: 

                3146    2495     257      78
 
BstYI r'GATC_y

Cuts at:      0     141     511    1938    2478    2854    2863    2964    4551 
   Size:        141     370    1427     540     376       9     101    1587

Cuts at:   4551    5364    5976 
   Size:        813     612

  Fragments arranged by size: 

                1587    1427     813     612     540     376     370     141
                 101       9
 
BstZ17I GTA'TAC

Cuts at:      0    4102    5976 
   Size:       4102    1874
 
BtgI C'CryG_G

Cuts at:      0     983    2858    5976 
   Size:        983    1875    3118

  Fragments arranged by size: 

                3118    1875     983
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3965    5976 
   Size:       3965    2011
 
BtsI GCAGTG_nn'

Cuts at:      0      34      56     228     933    2296    2889    3847    5976 
   Size:         34      22     172     705    1363     593     958    2129

  Fragments arranged by size: 

                2129    1363     958     705     593     172      34      22
            
 
DraI TTT'AAA

Cuts at:      0    3773    5976 
   Size:       3773    2203
 
DraIII CAC_nnn'GTG

Cuts at:      0     655    5976 
   Size:        655    5321
 
EaeI y'GGCC_r

Cuts at:      0    5111    5976 
   Size:       5111     865
 
EarI CTCTTCn'nnn_

Cuts at:      0     331     403     500     774     839    2760    3596    3609 
   Size:        331      72      97     274      65    1921     836      13

Cuts at:   3609    3918    4245    5976 
   Size:        309     327    1731

  Fragments arranged by size: 

                1921    1731     836     331     327     309     274      97
                  72      65      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     978    4819    5976 
   Size:        978    3841    1157

  Fragments arranged by size: 

                3841    1157     978
 
EcoICRI GAG'CTC

Cuts at:      0     948    5976 
   Size:        948    5028
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     268    1064    1088    1348    1450    1701    1903 
   Size:        146     122     796      24     260     102     251     202

Cuts at:   1903    2641    2692    2707    2745    2858    3343    3379    3617 
   Size:        738      51      15      38     113     485      36     238

Cuts at:   3617    4130    4915    5002    5431    5545    5728    5976 
   Size:        513     785      87     429     114     183     248

  Fragments arranged by size: 

                 796     785     738     513     485     429     260     251
                 248     238     202     183     146     122     114     113
                 102      87      51      38      36      24      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    5043    5777    5976 
   Size:       5043     734     199

  Fragments arranged by size: 

                5043     734     199
 
EcoO109I rG'GnC_Cy

Cuts at:      0      65    2307    2551    4821    5022    5976 
   Size:         65    2242     244    2270     201     954

  Fragments arranged by size: 

                2270    2242     954     244     201      65
 
EcoRI G'AATT_C

Cuts at:      0    1711    5976 
   Size:       1711    4265
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     829    1960    4984    5109    5976 
   Size:        829    1131    3024     125     867

  Fragments arranged by size: 

                3024    1131     867     829     125
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     237     269    1162    1194    1630    1662    1834    1866 
   Size:        237      32     893      32     436      32     172      32

Cuts at:   1866    2543    2575    2911    2943    3229    3261    3445    3477 
   Size:        677      32     336      32     286      32     184      32

Cuts at:   3477    3855    3887    3946    3978    4062    4094    4161    4193 
   Size:        378      32      59      32      84      32      67      32

Cuts at:   4193    5781    5813    5976 
   Size:       1588      32     163

  Fragments arranged by size: 

                1588     893     677     436     378     336     286     237
                 184     172     163      84      67      59      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1054    1357    2819    4523    5976 
   Size:       1054     303    1462    1704    1453

  Fragments arranged by size: 

                1704    1462    1453    1054     303
 
HindIII A'AGCT_T

Cuts at:      0    1208    3129    5976 
   Size:       1208    1921    2847

  Fragments arranged by size: 

                2847    1921    1208
 
HpaI GTT'AAC

Cuts at:      0    4523    5976 
   Size:       4523    1453
 
MfeI C'AATT_G

Cuts at:      0    1289    1748    5810    5976 
   Size:       1289     459    4062     166

  Fragments arranged by size: 

                4062    1289     459     166
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      29    2063    2071    3194    3253    3498    4291    4519 
   Size:         29    2034       8    1123      59     245     793     228

Cuts at:   4519    5757    5976 
   Size:       1238     219

  Fragments arranged by size: 

                2034    1238    1123     793     245     228     219      59
                  29       8
 
MscI TGG'CCA

Cuts at:      0    5113    5976 
   Size:       5113     863
 
MslI CAynn'nnrTG

Cuts at:      0    4072    5976 
   Size:       4072    1904
 
MspA1I CmG'CkG

Cuts at:      0    1042    1108    1962    2899    2917    3498    5976 
   Size:       1042      66     854     937      18     581    2478

  Fragments arranged by size: 

                2478    1042     937     854     581      66      18
 
NcoI C'CATG_G

Cuts at:      0    2858    5976 
   Size:       2858    3118
 
NsiI A_TGCA'T

Cuts at:      0    1743    2783    5328    5976 
   Size:       1743    1040    2545     648

  Fragments arranged by size: 

                2545    1743    1040     648
 
NspI r_CATG'y

Cuts at:      0    2022    2840    4438    5976 
   Size:       2022     818    1598    1538

  Fragments arranged by size: 

                2022    1598    1538     818
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3595    5976 
   Size:       3595    2381
 
PfoI T'CCnGG_A

Cuts at:      0    3594    5976 
   Size:       3594    2382
 
PmlI CAC'GTG

Cuts at:      0    2380    5976 
   Size:       2380    3596
 
PpuMI rG'GwC_Cy

Cuts at:      0    2551    4821    5976 
   Size:       2551    2270    1155

  Fragments arranged by size: 

                2551    2270    1155
 
PshAI GACnn'nnGTC

Cuts at:      0    4940    5812    5976 
   Size:       4940     872     164

  Fragments arranged by size: 

                4940     872     164
 
PsiI TTA'TAA

Cuts at:      0    2005    5168    5976 
   Size:       2005    3163     808

  Fragments arranged by size: 

                3163    2005     808
 
PspOMI G'GGCC_C

Cuts at:      0      65    5976 
   Size:         65    5911
 
PspXI vC'TCGA_Gb

Cuts at:      0    2456    5976 
   Size:       2456    3520
 
PstI C_TGCA'G

Cuts at:      0     106    1143    2916    4034    5976 
   Size:        106    1037    1773    1118    1942

  Fragments arranged by size: 

                1942    1773    1118    1037     106
 
PvuII CAG'CTG

Cuts at:      0    1042    1108    1962    2899    2917    3498    5976 
   Size:       1042      66     854     937      18     581    2478

  Fragments arranged by size: 

                2478    1042     937     854     581      66      18
 
SacI G_AGCT'C

Cuts at:      0     950    5976 
   Size:        950    5026
 
SalI G'TCGA_C

Cuts at:      0    1052    5976 
   Size:       1052    4924
 
SapI GCTCTTCn'nnn_

Cuts at:      0     774    3596    4245    5976 
   Size:        774    2822     649    1731

  Fragments arranged by size: 

                2822    1731     774     649
 
ScaI AGT'ACT

Cuts at:      0    3043    5566    5976 
   Size:       3043    2523     410

  Fragments arranged by size: 

                3043    2523     410
 
SexAI A'CCwGG_T

Cuts at:      0     651    5976 
   Size:        651    5325
 
SfcI C'TryA_G

Cuts at:      0     102     623    1139    1672    2912    4030    4924    5317 
   Size:        102     521     516     533    1240    1118     894     393

Cuts at:   5317    5646    5976 
   Size:        329     330

  Fragments arranged by size: 

                1240    1118     894     533     521     516     393     330
                 329     102
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3033    5976 
   Size:       3033    2943
 
SmlI C'TyrA_G

Cuts at:      0     206    2162    2456    2975    4730    5887    5976 
   Size:        206    1956     294     519    1755    1157      89

  Fragments arranged by size: 

                1956    1755    1157     519     294     206      89
 
SpeI A'CTAG_T

Cuts at:      0    2422    4498    5976 
   Size:       2422    2076    1478

  Fragments arranged by size: 

                2422    2076    1478
 
StuI AGG'CCT

Cuts at:      0    1624    5652    5976 
   Size:       1624    4028     324

  Fragments arranged by size: 

                4028    1624     324
 
StyI C'CwwG_G

Cuts at:      0    1131    1634    2858    5114    5151    5976 
   Size:       1131     503    1224    2256      37     825

  Fragments arranged by size: 

                2256    1224    1131     825     503      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     554    1995    2946    4691    5976 
   Size:        554    1441     951    1745    1285

  Fragments arranged by size: 

                1745    1441    1285     951     554
 
TatI w'GTAC_w

Cuts at:      0    3041    3160    3705    5564    5976 
   Size:       3041     119     545    1859     412

  Fragments arranged by size: 

                3041    1859     545     412     119
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1262    1294    2647    2679    5976 
   Size:       1262      32    1353      32    3297

  Fragments arranged by size: 

                3297    1353    1262      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    5263    5976 
   Size:       5263     713
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1288    3403    3615    5121    5752    5976 
   Size:       1288    2115     212    1506     631     224

  Fragments arranged by size: 

                2115    1506    1288     631     224     212
 
XhoI C'TCGA_G

Cuts at:      0     206    2456    5976 
   Size:        206    2250    3520

  Fragments arranged by size: 

                3520    2250     206
 
XmnI GAAnn'nnTTC

Cuts at:      0    1532    2090    3692    4490    5976 
   Size:       1532     558    1602     798    1486

  Fragments arranged by size: 

                1602    1532    1486     798     558
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI    AflII     AhdI     AlfI     AloI
    AlwNI     ApaI     ApoI     AseI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI   BstAPI    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     DraI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
   HincII  HindIII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I
     NcoI     NsiI     NspI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI    PvuII     SacI     SalI     SapI
     ScaI    SexAI     SfcI     SfiI     SmlI     SpeI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI   AflIII     AgeI     AleI    ApaLI     AscI
    AsiSI    AvrII     BaeI     BbeI     BcgI    BmgBI     BmtI     BsaI
    BsaHI    BsiEI    BsiWI    BsmBI    BsrBI    BsrGI   BssHII    BssSI
    BstBI   BstEII   Bsu36I     ClaI    CspCI     DrdI     EagI    EcoRV
     FseI     FspI    FspAI    HaeII     KasI     KpnI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PpiI     PsrI     PvuI    RsrII    SacII    SanDI
     SbfI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SspI     SwaI     XbaI     XmaI     ZraI
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